Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P12418

- MU2_REOVD

UniProt

P12418 - MU2_REOVD

Protein

Microtubule-associated protein mu-2

Gene

M1

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions By similarity.By similarity

    Cofactori

    Divalent cations.By similarity

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. suppression by virus of host IRF9 activity Source: UniProtKB-KW
    2. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF9 by virus, Viral immunoevasion

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Microtubule-associated protein mu-2
    Short name:
    Mu2
    Gene namesi
    Name:M1
    OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
    Taxonomic identifieri10886 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000006373: Genome

    Subcellular locationi

    Virion Curated. Host cytoplasmhost cytoskeleton 1 Publication
    Note: Found in the inner capsid (12 copies).

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-KW
    2. host cytoskeleton Source: UniProtKB-SubCell
    3. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cytoplasm, Host cytoskeleton, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi208 – 2081P → S: Loss of filamentous subcellular location. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 736736Microtubule-associated protein mu-2PRO_0000222746Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP12418. 9 interactions.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the orthoreovirus mu-2 protein family.Curated

    Family and domain databases

    InterProiIPR012494. Reovirus_Mu2.
    [Graphical view]
    PfamiPF07781. Reovirus_Mu2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12418-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM    50
    LDGYEAGDVI DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSAIPDNVDR 100
    RLRKRLMLKK DLRKDDEYNQ LARAFKISDV YAPLISSTTS PMTMIQNLNR 150
    GEIVYTTTDR VIGARILLYA PRKYYASTLS FTMTKCIIPF GKEVGRVPHS 200
    RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS VHANILNDIS 250
    PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDM 300
    LFEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMLTDW 350
    FLLLTMLSDG LTDRRTHCQY LMNPSSVPPD VILNISITGF INRHTIDVMP 400
    DIYDFVKPIG AVLPKGSFKS TIMRVLDSIS ILGIQIMPRA HVVDSDEVGE 450
    QMEPTFEQAV MEIYKGIAGV DSLDDLIKWV LNSDLIPHDD RLGQLFQAFL 500
    PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF GHLLRLKIPY 550
    ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL 600
    HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL 650
    VIEELMEKNW GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF 700
    ACFTLTAKYE MRLSCGHSTG RGAAYSARLA FRSDLA 736
    Length:736
    Mass (Da):83,276
    Last modified:October 1, 1989 - v1
    Checksum:i2CF0D88BA16EF564
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti150 – 1501R → Q in AAL99937. (PubMed:11932414)Curated
    Sequence conflicti150 – 1501R → Q in AAS55892. (PubMed:15507160)Curated
    Sequence conflicti150 – 1501R → Q in ABP48916. (PubMed:18005692)Curated
    Sequence conflicti208 – 2081P → S in AAL99937. (PubMed:11932414)Curated
    Sequence conflicti208 – 2081P → S in AAS55892. (PubMed:15507160)Curated
    Sequence conflicti208 – 2081P → S in ABP48916. (PubMed:18005692)Curated
    Sequence conflicti342 – 3421Q → R in ABP48916. (PubMed:18005692)Curated
    Sequence conflicti372 – 3721M → I in AAL99937. (PubMed:11932414)Curated
    Sequence conflicti372 – 3721M → I in AAS55892. (PubMed:15507160)Curated
    Sequence conflicti372 – 3721M → I in ABP48916. (PubMed:18005692)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27261 Genomic RNA. Translation: AAA47256.1.
    AF461683 mRNA. Translation: AAL99937.1.
    AF461684 mRNA. Translation: AAL99938.1.
    AY551083 mRNA. Translation: AAS55892.1.
    EF494438 Genomic RNA. Translation: ABP48916.1.
    PIRiA30179. M4XR3D.
    RefSeqiNP_694685.1. NC_004280.1.

    Genome annotation databases

    GeneIDi956474.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27261 Genomic RNA. Translation: AAA47256.1 .
    AF461683 mRNA. Translation: AAL99937.1 .
    AF461684 mRNA. Translation: AAL99938.1 .
    AY551083 mRNA. Translation: AAS55892.1 .
    EF494438 Genomic RNA. Translation: ABP48916.1 .
    PIRi A30179. M4XR3D.
    RefSeqi NP_694685.1. NC_004280.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P12418. 9 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956474.

    Family and domain databases

    InterProi IPR012494. Reovirus_Mu2.
    [Graphical view ]
    Pfami PF07781. Reovirus_Mu2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively."
      Wiener J.R., Bartlett J.A., Joklik W.K.
      Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules."
      Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L.
      J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-208.
      Strain: Isolate T3/Human/Ohio/1955.
    3. "Comparisons of the M1 genome segments and encoded mu2 proteins of different reovirus isolates."
      Yin P., Keirstead N.D., Broering T.J., Arnold M.M., Parker J.S.L., Nibert M.L., Coombs K.M.
      Virol. J. 1:6-6(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Isolate T3/Human/Washington.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Infectious clone.

    Entry informationi

    Entry nameiMU2_REOVD
    AccessioniPrimary (citable) accession number: P12418
    Secondary accession number(s): A4ZY23, Q71M35, Q8QT10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3