P12418 (MU2_REOVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Microtubule-associated protein mu-2
Short name=Mu2

Gene names

Name:

M1

Organism

Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]

Taxonomic identifier

10886 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 3

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	736 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions By similarity.
Cofactor	Divalent cations By similarity.
Subunit structure	Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2 By similarity.
Subcellular location	Virion Potential. Host cytoplasm › host cytoskeleton. Note: Found in the inner capsid (12 copies). Ref.2
Sequence similarities	Belongs to the orthoreovirus mu-2 protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host IRF9 by virus Inhibition of host innate immune response by virus Inhibition of host interferon signaling pathway by virus Viral immunoevasion
Cellular component	Host cytoplasm Host cytoskeleton Virion
Molecular function	Capsid protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host IRF9 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 736	736	Microtubule-associated protein mu-2		PRO_0000222746
Experimental info
	Mutagenesis	208	1	P → S: Loss of filamentous subcellular location. Ref.2
	Sequence conflict	150	1	R → Q in AAL99937. Ref.2
	Sequence conflict	150	1	R → Q in AAS55892. Ref.3
	Sequence conflict	150	1	R → Q in ABP48916. Ref.4
	Sequence conflict	208	1	P → S in AAL99937. Ref.2
	Sequence conflict	208	1	P → S in AAS55892. Ref.3
	Sequence conflict	208	1	P → S in ABP48916. Ref.4
	Sequence conflict	342	1	Q → R in ABP48916. Ref.4
	Sequence conflict	372	1	M → I in AAL99937. Ref.2
	Sequence conflict	372	1	M → I in AAS55892. Ref.3
	Sequence conflict	372	1	M → I in ABP48916. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P12418 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 2CF0D88BA16EF564	FASTA	736	83,276
	10 20 30 40 50 60 MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM LDGYEAGDVI 70 80 90 100 110 120 DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSAIPDNVDR RLRKRLMLKK DLRKDDEYNQ 130 140 150 160 170 180 LARAFKISDV YAPLISSTTS PMTMIQNLNR GEIVYTTTDR VIGARILLYA PRKYYASTLS 190 200 210 220 230 240 FTMTKCIIPF GKEVGRVPHS RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS 250 260 270 280 290 300 VHANILNDIS PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDM 310 320 330 340 350 360 LFEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMLTDW FLLLTMLSDG 370 380 390 400 410 420 LTDRRTHCQY LMNPSSVPPD VILNISITGF INRHTIDVMP DIYDFVKPIG AVLPKGSFKS 430 440 450 460 470 480 TIMRVLDSIS ILGIQIMPRA HVVDSDEVGE QMEPTFEQAV MEIYKGIAGV DSLDDLIKWV 490 500 510 520 530 540 LNSDLIPHDD RLGQLFQAFL PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF 550 560 570 580 590 600 GHLLRLKIPY ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL 610 620 630 640 650 660 HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL VIEELMEKNW 670 680 690 700 710 720 GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF ACFTLTAKYE MRLSCGHSTG 730 RGAAYSARLA FRSDLA

References

[1]	"The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively." Wiener J.R., Bartlett J.A., Joklik W.K. Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules." Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L. J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-208. Strain: Isolate T3/Human/Ohio/1955.
[3]	"Comparisons of the M1 genome segments and encoded mu2 proteins of different reovirus isolates." Yin P., Keirstead N.D., Broering T.J., Arnold M.M., Parker J.S.L., Nibert M.L., Coombs K.M. Virol. J. 1:6-6(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Isolate T3/Human/Washington.
[4]	"A plasmid-based reverse genetics system for animal double-stranded RNA viruses." Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S. Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Infectious clone.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27261 Genomic RNA. Translation: AAA47256.1. AF461683 mRNA. Translation: AAL99937.1. AF461684 mRNA. Translation: AAL99938.1. AY551083 mRNA. Translation: AAS55892.1. EF494438 Genomic RNA. Translation: ABP48916.1.
PIR	M4XR3D. A30179.
RefSeq	NP_694685.1. NC_004280.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P12418. 9 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	956474.
Family and domain databases
InterPro	IPR012494. Reovirus_Mu2. [Graphical view]
Pfam	PF07781. Reovirus_Mu2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MU2_REOVD

Accession

Primary (citable) accession number: P12418
Secondary accession number(s): A4ZY23, Q71M35, Q8QT10

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program