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P12418

- MU2_REOVD

UniProt

P12418 - MU2_REOVD

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Protein

Microtubule-associated protein mu-2

Gene

M1

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions (By similarity).By similarity

Cofactori

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. suppression by virus of host IRF9 activity Source: UniProtKB-KW
  2. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF9 by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein mu-2
Short name:
Mu2
Gene namesi
Name:M1
OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Taxonomic identifieri10886 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006373: Genome

Subcellular locationi

Virion Curated. Host cytoplasmhost cytoskeleton 1 Publication
Note: Found in the inner capsid (12 copies).

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-KW
  3. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Host cytoskeleton, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi208 – 2081P → S: Loss of filamentous subcellular location. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736Microtubule-associated protein mu-2PRO_0000222746Add
BLAST

Interactioni

Subunit structurei

Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2 (By similarity).By similarity

Protein-protein interaction databases

IntActiP12418. 9 interactions.

Family & Domainsi

Sequence similaritiesi

Belongs to the orthoreovirus mu-2 protein family.Curated

Family and domain databases

InterProiIPR012494. Reovirus_Mu2.
[Graphical view]
PfamiPF07781. Reovirus_Mu2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12418-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM
60 70 80 90 100
LDGYEAGDVI DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSAIPDNVDR
110 120 130 140 150
RLRKRLMLKK DLRKDDEYNQ LARAFKISDV YAPLISSTTS PMTMIQNLNR
160 170 180 190 200
GEIVYTTTDR VIGARILLYA PRKYYASTLS FTMTKCIIPF GKEVGRVPHS
210 220 230 240 250
RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS VHANILNDIS
260 270 280 290 300
PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDM
310 320 330 340 350
LFEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMLTDW
360 370 380 390 400
FLLLTMLSDG LTDRRTHCQY LMNPSSVPPD VILNISITGF INRHTIDVMP
410 420 430 440 450
DIYDFVKPIG AVLPKGSFKS TIMRVLDSIS ILGIQIMPRA HVVDSDEVGE
460 470 480 490 500
QMEPTFEQAV MEIYKGIAGV DSLDDLIKWV LNSDLIPHDD RLGQLFQAFL
510 520 530 540 550
PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF GHLLRLKIPY
560 570 580 590 600
ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL
610 620 630 640 650
HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL
660 670 680 690 700
VIEELMEKNW GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF
710 720 730
ACFTLTAKYE MRLSCGHSTG RGAAYSARLA FRSDLA
Length:736
Mass (Da):83,276
Last modified:October 1, 1989 - v1
Checksum:i2CF0D88BA16EF564
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501R → Q in AAL99937. (PubMed:11932414)Curated
Sequence conflicti150 – 1501R → Q in AAS55892. (PubMed:15507160)Curated
Sequence conflicti150 – 1501R → Q in ABP48916. (PubMed:18005692)Curated
Sequence conflicti208 – 2081P → S in AAL99937. (PubMed:11932414)Curated
Sequence conflicti208 – 2081P → S in AAS55892. (PubMed:15507160)Curated
Sequence conflicti208 – 2081P → S in ABP48916. (PubMed:18005692)Curated
Sequence conflicti342 – 3421Q → R in ABP48916. (PubMed:18005692)Curated
Sequence conflicti372 – 3721M → I in AAL99937. (PubMed:11932414)Curated
Sequence conflicti372 – 3721M → I in AAS55892. (PubMed:15507160)Curated
Sequence conflicti372 – 3721M → I in ABP48916. (PubMed:18005692)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27261 Genomic RNA. Translation: AAA47256.1.
AF461683 mRNA. Translation: AAL99937.1.
AF461684 mRNA. Translation: AAL99938.1.
AY551083 mRNA. Translation: AAS55892.1.
EF494438 Genomic RNA. Translation: ABP48916.1.
PIRiA30179. M4XR3D.
RefSeqiNP_694685.1. NC_004280.1.

Genome annotation databases

GeneIDi956474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27261 Genomic RNA. Translation: AAA47256.1 .
AF461683 mRNA. Translation: AAL99937.1 .
AF461684 mRNA. Translation: AAL99938.1 .
AY551083 mRNA. Translation: AAS55892.1 .
EF494438 Genomic RNA. Translation: ABP48916.1 .
PIRi A30179. M4XR3D.
RefSeqi NP_694685.1. NC_004280.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P12418. 9 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956474.

Family and domain databases

InterProi IPR012494. Reovirus_Mu2.
[Graphical view ]
Pfami PF07781. Reovirus_Mu2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively."
    Wiener J.R., Bartlett J.A., Joklik W.K.
    Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules."
    Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L.
    J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-208.
    Strain: Isolate T3/Human/Ohio/1955.
  3. "Comparisons of the M1 genome segments and encoded mu2 proteins of different reovirus isolates."
    Yin P., Keirstead N.D., Broering T.J., Arnold M.M., Parker J.S.L., Nibert M.L., Coombs K.M.
    Virol. J. 1:6-6(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Isolate T3/Human/Washington.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Infectious clone.

Entry informationi

Entry nameiMU2_REOVD
AccessioniPrimary (citable) accession number: P12418
Secondary accession number(s): A4ZY23, Q71M35, Q8QT10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3