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P12418 (MU2_REOVD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Microtubule-associated protein mu-2

Short name=Mu2
Gene names
Name:M1
OrganismReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]
Taxonomic identifier10886 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Minor inner capsid (core) component. Displays NTPase and RNA 5'-triphosphatase (RTPase) activities. ATP is the preferred substrate for hydrolysis. May function as a cofactor of polymerase lambda-3. Associates with microtubules and plays a role in the formation, structural organization and morphology of viral inclusions, where the assembly of cores and the replication of viral RNA occur. Together with mu-NS, recruits the other core proteins to these inclusions By similarity.

Cofactor

Divalent cations By similarity.

Subunit structure

Interacts with protein mu-NS; in viral inclusions. Interacts with polymerase lambda-3; this interaction stimulates the ATPase activity of mu-2 By similarity.

Subcellular location

Virion Potential. Host cytoplasmhost cytoskeleton. Note: Found in the inner capsid (12 copies). Ref.2

Sequence similarities

Belongs to the orthoreovirus mu-2 protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 736736Microtubule-associated protein mu-2
PRO_0000222746

Experimental info

Mutagenesis2081P → S: Loss of filamentous subcellular location. Ref.2
Sequence conflict1501R → Q in AAL99937. Ref.2
Sequence conflict1501R → Q in AAS55892. Ref.3
Sequence conflict1501R → Q in ABP48916. Ref.4
Sequence conflict2081P → S in AAL99937. Ref.2
Sequence conflict2081P → S in AAS55892. Ref.3
Sequence conflict2081P → S in ABP48916. Ref.4
Sequence conflict3421Q → R in ABP48916. Ref.4
Sequence conflict3721M → I in AAL99937. Ref.2
Sequence conflict3721M → I in AAS55892. Ref.3
Sequence conflict3721M → I in ABP48916. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P12418 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 2CF0D88BA16EF564

FASTA73683,276
        10         20         30         40         50         60 
MAYIAVPAVV DSRSSEAIGL LESFGVDAGA DANDVSYQDH DYVLDQLQYM LDGYEAGDVI 

        70         80         90        100        110        120 
DALVHKNWLH HSVYCLLPPK SQLLEYWKSN PSAIPDNVDR RLRKRLMLKK DLRKDDEYNQ 

       130        140        150        160        170        180 
LARAFKISDV YAPLISSTTS PMTMIQNLNR GEIVYTTTDR VIGARILLYA PRKYYASTLS 

       190        200        210        220        230        240 
FTMTKCIIPF GKEVGRVPHS RFNVGTFPSI ATPKCFVMSG VDIESIPNEF IKLFYQRVKS 

       250        260        270        280        290        300 
VHANILNDIS PQIVSDMINR KRLRVHTPSD RRAAQLMHLP YHVKRGASHV DVYKVDVVDM 

       310        320        330        340        350        360 
LFEVVDVADG LRNVSRKLTM HTVPVCILEM LGIEIADYCI RQEDGMLTDW FLLLTMLSDG 

       370        380        390        400        410        420 
LTDRRTHCQY LMNPSSVPPD VILNISITGF INRHTIDVMP DIYDFVKPIG AVLPKGSFKS 

       430        440        450        460        470        480 
TIMRVLDSIS ILGIQIMPRA HVVDSDEVGE QMEPTFEQAV MEIYKGIAGV DSLDDLIKWV 

       490        500        510        520        530        540 
LNSDLIPHDD RLGQLFQAFL PLAKDLLAPM ARKFYDNSMS EGRLLTFAHA DSELLNANYF 

       550        560        570        580        590        600 
GHLLRLKIPY ITEVNLMIRK NREGGELFQL VLSYLYKMYA TSAQPKWFGS LLRLLICPWL 

       610        620        630        640        650        660 
HMEKLIGEAD PASTSAEIGW HIPREQLMQD GWCGCEDGFI PYVSIRAPRL VIEELMEKNW 

       670        680        690        700        710        720 
GQYHAQVIVT DQLVVGEPRR VSAKAVIKGN HLPVKLVSRF ACFTLTAKYE MRLSCGHSTG 

       730 
RGAAYSARLA FRSDLA 

« Hide

References

[1]"The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively."
Wiener J.R., Bartlett J.A., Joklik W.K.
Virology 169:293-304(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Reovirus core protein mu2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules."
Parker J.S.L., Broering T.J., Kim J., Higgins D.E., Nibert M.L.
J. Virol. 76:4483-4496(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, MUTAGENESIS OF PRO-208.
Strain: Isolate T3/Human/Ohio/1955.
[3]"Comparisons of the M1 genome segments and encoded mu2 proteins of different reovirus isolates."
Yin P., Keirstead N.D., Broering T.J., Arnold M.M., Parker J.S.L., Nibert M.L., Coombs K.M.
Virol. J. 1:6-6(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Isolate T3/Human/Washington.
[4]"A plasmid-based reverse genetics system for animal double-stranded RNA viruses."
Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S.
Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Infectious clone.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27261 Genomic RNA. Translation: AAA47256.1.
AF461683 mRNA. Translation: AAL99937.1.
AF461684 mRNA. Translation: AAL99938.1.
AY551083 mRNA. Translation: AAS55892.1.
EF494438 Genomic RNA. Translation: ABP48916.1.
PIRM4XR3D. A30179.
RefSeqNP_694685.1. NC_004280.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP12418. 9 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956474.

Family and domain databases

InterProIPR012494. Reovirus_Mu2.
[Graphical view]
PfamPF07781. Reovirus_Mu2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMU2_REOVD
AccessionPrimary (citable) accession number: P12418
Secondary accession number(s): A4ZY23, Q71M35, Q8QT10
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families