P12417 (ANP2_ANALU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Type-3 ice-structuring protein 1.5 Alternative name(s): Antifreeze protein type III ISP 3
Organism	Anarhichas lupus (Atlantic wolffish)
Taxonomic identifier	8204 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Perciformes › Zoarcoidei › Anarhichadidae › Anarhichas

Protein attributes

Sequence length	88 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Antifreeze proteins lower the blood freezing point by absorbing ice and inhibiting its growth.
Subcellular location	Secreted.
Sequence similarities	Belongs to the type-III AFP family. Contains 1 AFP-like domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antifreeze protein
Gene Ontology (GO)
Biological_process	response to freezing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 88	65	Type-3 ice-structuring protein 1.5		PRO_0000001697
Regions
	Domain	25 – 84	60	AFP-like
Sites
	Site	30	1	Important for ice-binding By similarity
	Site	35	1	Important for ice-binding By similarity
	Site	39	1	Important for ice-binding By similarity
	Site	65	1	Important for ice-binding By similarity
Experimental info
	Sequence conflict	24	1	Q → QQ in AAA74891. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12417 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 72CAFCFA474E85CC	FASTA	88	9,430
	10 20 30 40 50 60 MKSAILTGLL FVLLCVDHMS SASQSVVATQ LIPINTALTP IMMKGQVVNP AGIPFAEMSQ 70 80 IVGKQVNRAV AKDETLMPNM VKTYRAAK

References

[1]	"Wolffish antifreeze protein genes are primarily organized as tandem repeats that each contain two genes in inverted orientation." Scott G.K., Hayes P.H., Fletcher G.L., Davies P.L. Mol. Cell. Biol. 8:3670-3675(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22125 Genomic DNA. Translation: AAA74891.1.
3D structure databases
ProteinModelPortal	P12417.
SMR	P12417. Positions 23-86.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG004237.
Family and domain databases
Gene3D	3.90.1210.10. 1 hit.
InterPro	IPR006190. AFP_Neu5c_C. IPR006013. Antifreeze_III. IPR013974. SAF. [Graphical view]
Pfam	PF08666. SAF. 1 hit. [Graphical view]
PRINTS	PR00357. ANTIFREEZIII.
SMART	SM00858. SAF. 1 hit. [Graphical view]
SUPFAM	SSF51269. AFP_Neu5c_C. 1 hit.
PROSITE	PS50844. AFP_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANP2_ANALU

Accession

Primary (citable) accession number: P12417

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program