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P12397 (MU1_REOVJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein mu-1

Short name=Mu1

Cleaved into the following 2 chains:

  1. Outer capsid protein mu-1N
  2. Outer capsid protein mu-1C
Gene names
Name:M2
OrganismReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2) [Complete proteome]
Taxonomic identifier10885 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length708 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major outer capsid protein involved in host cell membrane penetration. In the endocytic compartment, outer-capsid protein sigma-3 is removed by cathepsin proteases, which exposes the viral membrane-penetration protein mu-1. Both myristoylated peptides mu-1N and phi are released during infectious subvirion particles (ISVP) formation in the endosome. They associate with host membranes and mu-1N induces permeabilization and delivery of transcriptionally active viral particles into the host cell cytoplasm. Seems to induce apoptosis in the host cell By similarity.

The viral outer shell polypeptides, of which mu-1 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3 By similarity.

Subunit structure

Heterohexamer of three sigma-3 and three Mu-1 proteins By similarity.

Subcellular location

Outer capsid protein mu-1: Virion By similarity. Host cell membrane; Lipid-anchor By similarity. Host endoplasmic reticulum By similarity. Host mitochondrion By similarity. Note: Found in the outer capsid. Seems to associate with cell membranes. This association is enhanced by myristoylation By similarity.

Domain

The C-terminal region, phi, determines both targeting to intracellular membranes and induction of apoptosis By similarity.

Post-translational modification

Cleaved during the endosomal proteolytic disassembly of the outer capsid. Mu-1 is proteolytically cleaved into mu-1N and mu-1C during the maturation step to generate the ISVP. Cleavage of mu-1 to mu-1C is dependent on myristoylation and binding to sigma-3 protein. Mu-1C is further cleaved into delta (59 kDa), and phi (13 kDa) segments during entry into the host cell cytoplasm. Ref.2

Mu-1 and mu-1N are N-terminally myristoylated. This acylation is essential for the membrane fusion activity By similarity.

Sequence similarities

Belongs to the orthoreovirus mu-1 protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host By similarity
Chain2 – 708707Outer capsid protein mu-1
PRO_0000040659
Chain2 – 4241Outer capsid protein mu-1N
PRO_0000344979
Chain43 – 708666Outer capsid protein mu-1C
PRO_0000040661

Sites

Site42 – 432Cleavage By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine; by host By similarity
Glycosylation31N-linked (GlcNAc...); by host Potential
Glycosylation121N-linked (GlcNAc...); by host Potential
Glycosylation811N-linked (GlcNAc...); by host Potential
Glycosylation1101N-linked (GlcNAc...); by host Potential
Glycosylation4581N-linked (GlcNAc...); by host Potential
Glycosylation4821N-linked (GlcNAc...); by host Potential
Glycosylation5281N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P12397 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 503DEE3053DEF422

FASTA70876,146
        10         20         30         40         50         60 
MGNASSIVQT INVTGDGNVF KPSAETSSTA VPSLSLSPGM LNPGGVPWIA IGDETSVTSP 

        70         80         90        100        110        120 
GALRRMTSKD IPETAIINTD NSSGAVPSES ALVPYNDEPL VVVTEHAIAN FTKAEMALEF 

       130        140        150        160        170        180 
NREFLDKLRV LSVSPKYSDL LTYVDCYVGV SARQALNNFQ KQVPVITPTR QTMYVDSIQA 

       190        200        210        220        230        240 
ALKALEKWEI DLRVAQTLLP TNVPIGEVSC PMQSVVKLLD DQLPDDSLIR RYPKEAAVAL 

       250        260        270        280        290        300 
AKRNGGIQWM DVSEGTVMNE AVNAVAASAL APSASAPPLE EKSKLTEQAM DLVTAAEPEI 

       310        320        330        340        350        360 
IASLVPVPAP VFAIPPKPAD YNVRTLKIDE ATWLRMIPKT MNTPFQIQVT DNTGTSWHMN 

       370        380        390        400        410        420 
LRGGTRVVNL DQIAPMRFVL DLGGKSYKET SWDPNGKKVG FIVFQSKIPF ELWTAASQIG 

       430        440        450        460        470        480 
QATVVNYVQL YAEDSSFTAQ SIIATTSLAY NYEPEQLNKT DPEMNYYLLA AFIDSAAIST 

       490        500        510        520        530        540 
SNMTQPDVWD ALLTMSPLSA GEVTVKGAVV SEVIPADLVG SYTPESLNAS LPNDAARCMI 

       550        560        570        580        590        600 
DRASKIAEAI KIDDDAGPDE YSPNSVPIQG QLAISQLETG YGVRIFNPKG ILSKIASRAM 

       610        620        630        640        650        660 
QAFIGDPSTI ITQAAPVLSD KNNWIALAQG VKTSLRTKSL SAGVKTAVSK LSSSESIQSW 

       670        680        690        700 
TQGFLDKVST HFPAPKPDCP QSGDSGDGSA RRLKRDSYAG VVKRGYTR 

« Hide

References

[1]"Evolution of reovirus genes: a comparison of serotype 1, 2, and 3 M2 genome segments, which encode the major structural capsid protein mu 1C."
Wiener J.R., Joklik W.K.
Virology 163:603-613(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A carboxy-terminal fragment of protein mu 1/mu 1C is present in infectious subvirion particles of mammalian reoviruses and is proposed to have a role in penetration."
Nibert M.L., Fields B.N.
J. Virol. 66:6408-6418(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19355 mRNA. Translation: AAA47246.1.
PIRM2XR2J. B28607.

3D structure databases

ProteinModelPortalP12397.
SMRP12397. Positions 10-675.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB1.A.60.1.1. the mammalian reovirus pre-forming peptide, mu-1 (mu-1) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.2040.10. 2 hits.
2.60.120.420. 1 hit.
3.90.1370.10. 1 hit.
InterProIPR009113. Mu1/VP4.
IPR015962. Mu1_membr_pen_a-bundle_sub2.
IPR015961. Mu1_membr_pen_a/b_sub.
IPR015960. Mu1_membr_pen_b-sand_sub.
[Graphical view]
PfamPF05993. Reovirus_M2. 1 hit.
[Graphical view]
SUPFAMSSF69908. SSF69908. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMU1_REOVJ
AccessionPrimary (citable) accession number: P12397
Secondary accession number(s): Q85659, Q89834
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families