Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P12397

- MU1_REOVJ

UniProt

P12397 - MU1_REOVJ

Protein

Outer capsid protein mu-1

Gene

M2

Organism
Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Major outer capsid protein involved in host cell membrane penetration. In the endocytic compartment, outer-capsid protein sigma-3 is removed by cathepsin proteases, which exposes the viral membrane-penetration protein mu-1. Both myristoylated peptides mu-1N and phi are released during infectious subvirion particles (ISVP) formation in the endosome. They associate with host membranes and mu-1N induces permeabilization and delivery of transcriptionally active viral particles into the host cell cytoplasm. Seems to induce apoptosis in the host cell By similarity.By similarity
    The viral outer shell polypeptides, of which mu-1 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei42 – 432CleavageBy similarity

    GO - Molecular functioni

    1. host cell surface binding Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. pathogenesis Source: InterPro
    3. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Apoptosis, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Protein family/group databases

    TCDBi1.A.60.1.1. the mammalian reovirus pre-forming peptide, mu-1 (mu-1) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein mu-1
    Short name:
    Mu1
    Cleaved into the following 2 chains:
    Gene namesi
    Name:M2
    OrganismiReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
    Taxonomic identifieri10885 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000006370: Genome

    Subcellular locationi

    Chain Outer capsid protein mu-1 : Virion By similarity. Host cell membrane By similarity; Lipid-anchor By similarity. Host endoplasmic reticulum By similarity. Host mitochondrion By similarity
    Note: Found in the outer capsid. Seems to associate with cell membranes. This association is enhanced by myristoylation By similarity.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum Source: UniProtKB-SubCell
    2. host cell mitochondrion Source: UniProtKB-SubCell
    3. host cell plasma membrane Source: UniProtKB-SubCell
    4. membrane Source: UniProtKB-KW
    5. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cell membrane, Host endoplasmic reticulum, Host membrane, Host mitochondrion, Membrane, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by hostBy similarity
    Chaini2 – 708707Outer capsid protein mu-1PRO_0000040659Add
    BLAST
    Chaini2 – 4241Outer capsid protein mu-1NPRO_0000344979Add
    BLAST
    Chaini43 – 708666Outer capsid protein mu-1CPRO_0000040661Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity
    Glycosylationi3 – 31N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi12 – 121N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi81 – 811N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi110 – 1101N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi458 – 4581N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi482 – 4821N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi528 – 5281N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    Cleaved during the endosomal proteolytic disassembly of the outer capsid. Mu-1 is proteolytically cleaved into mu-1N and mu-1C during the maturation step to generate the ISVP. Cleavage of mu-1 to mu-1C is dependent on myristoylation and binding to sigma-3 protein. Mu-1C is further cleaved into delta (59 kDa), and phi (13 kDa) segments during entry into the host cell cytoplasm.1 Publication
    Mu-1 and mu-1N are N-terminally myristoylated. This acylation is essential for the membrane fusion activity By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Myristate

    Interactioni

    Subunit structurei

    Heterohexamer of three sigma-3 and three Mu-1 proteins.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP12397.
    SMRiP12397. Positions 10-675.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The C-terminal region, phi, determines both targeting to intracellular membranes and induction of apoptosis.By similarity

    Sequence similaritiesi

    Belongs to the orthoreovirus mu-1 protein family.Curated

    Family and domain databases

    Gene3Di1.10.2040.10. 2 hits.
    2.60.120.420. 1 hit.
    3.90.1370.10. 1 hit.
    InterProiIPR009113. Mu1/VP4.
    IPR015962. Mu1_membr_pen_a-bundle_sub2.
    IPR015961. Mu1_membr_pen_a/b_sub.
    IPR015960. Mu1_membr_pen_b-sand_sub.
    [Graphical view]
    PfamiPF05993. Reovirus_M2. 1 hit.
    [Graphical view]
    SUPFAMiSSF69908. SSF69908. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12397-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNASSIVQT INVTGDGNVF KPSAETSSTA VPSLSLSPGM LNPGGVPWIA    50
    IGDETSVTSP GALRRMTSKD IPETAIINTD NSSGAVPSES ALVPYNDEPL 100
    VVVTEHAIAN FTKAEMALEF NREFLDKLRV LSVSPKYSDL LTYVDCYVGV 150
    SARQALNNFQ KQVPVITPTR QTMYVDSIQA ALKALEKWEI DLRVAQTLLP 200
    TNVPIGEVSC PMQSVVKLLD DQLPDDSLIR RYPKEAAVAL AKRNGGIQWM 250
    DVSEGTVMNE AVNAVAASAL APSASAPPLE EKSKLTEQAM DLVTAAEPEI 300
    IASLVPVPAP VFAIPPKPAD YNVRTLKIDE ATWLRMIPKT MNTPFQIQVT 350
    DNTGTSWHMN LRGGTRVVNL DQIAPMRFVL DLGGKSYKET SWDPNGKKVG 400
    FIVFQSKIPF ELWTAASQIG QATVVNYVQL YAEDSSFTAQ SIIATTSLAY 450
    NYEPEQLNKT DPEMNYYLLA AFIDSAAIST SNMTQPDVWD ALLTMSPLSA 500
    GEVTVKGAVV SEVIPADLVG SYTPESLNAS LPNDAARCMI DRASKIAEAI 550
    KIDDDAGPDE YSPNSVPIQG QLAISQLETG YGVRIFNPKG ILSKIASRAM 600
    QAFIGDPSTI ITQAAPVLSD KNNWIALAQG VKTSLRTKSL SAGVKTAVSK 650
    LSSSESIQSW TQGFLDKVST HFPAPKPDCP QSGDSGDGSA RRLKRDSYAG 700
    VVKRGYTR 708
    Length:708
    Mass (Da):76,146
    Last modified:January 23, 2007 - v3
    Checksum:i503DEE3053DEF422
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19355 mRNA. Translation: AAA47246.1.
    PIRiB28607. M2XR2J.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19355 mRNA. Translation: AAA47246.1 .
    PIRi B28607. M2XR2J.

    3D structure databases

    ProteinModelPortali P12397.
    SMRi P12397. Positions 10-675.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    TCDBi 1.A.60.1.1. the mammalian reovirus pre-forming peptide, mu-1 (mu-1) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.2040.10. 2 hits.
    2.60.120.420. 1 hit.
    3.90.1370.10. 1 hit.
    InterProi IPR009113. Mu1/VP4.
    IPR015962. Mu1_membr_pen_a-bundle_sub2.
    IPR015961. Mu1_membr_pen_a/b_sub.
    IPR015960. Mu1_membr_pen_b-sand_sub.
    [Graphical view ]
    Pfami PF05993. Reovirus_M2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69908. SSF69908. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of reovirus genes: a comparison of serotype 1, 2, and 3 M2 genome segments, which encode the major structural capsid protein mu 1C."
      Wiener J.R., Joklik W.K.
      Virology 163:603-613(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "A carboxy-terminal fragment of protein mu 1/mu 1C is present in infectious subvirion particles of mammalian reoviruses and is proposed to have a role in penetration."
      Nibert M.L., Fields B.N.
      J. Virol. 66:6408-6418(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEOLYTIC PROCESSING.

    Entry informationi

    Entry nameiMU1_REOVJ
    AccessioniPrimary (citable) accession number: P12397
    Secondary accession number(s): Q85659, Q89834
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 88 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3