Reviewed, UniProtKB/Swiss-Prot P12394 (CP17A_CHICK)

Names and origin

Protein names

Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase
    EC=1.14.99.9
Alternative name(s):
    Cytochrome P450 17A1
    CYPXVII
    P450-C17
      Short name=P450c17

Gene names

Name:	CYP17A1
Synonyms:	CYP17

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.
Catalytic activity	A steroid + AH2 + O2 = a 17-alpha-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051943
Sites
	Metal binding	445	1	Iron (heme axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	P12394-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 52BE915B46E237AF	FASTA	508	56,984
	10 20 30 40 50 60 MPPLAVLLLA LALLCAWRLS YSQGPTGTGT GRPRSLPALP LVGSLLQLAG HPQLHLRLWR 70 80 90 100 110 120 LQGRYGSLYG LWMGSHYVVV VNSYQHAREV LLKKGKAFAG RPRTVTTDLL SRGGKDIAFA 130 140 150 160 170 180 SYGPLWKFQR KLVHAALSMF GEGSVALEKI ICREAASLCE TLGAAQDMAL DMAPELTRAV 190 200 210 220 230 240 TNVVCSLCFN SSYRRGDPEF EAMLEYSQGI VDTVAKESLV DIFPWLQIFP NRDLALLKRC 250 260 270 280 290 300 LKVRDQLLQQ KFTEHKEAFC GDTVRDLMDA LLQVRLNAEN NSPLEPGLEL TDDHLLMTVG 310 320 330 340 350 360 DIFGAGVETT TTVLKWAVLY LLHYPEVQKK IQEEMDQKIG LARHPHLSDR PLLPYLEATI 370 380 390 400 410 420 SEGLRIRPVS PLLIPHVSLA DTSIGEYSIP KGARVVINLW SVHHDEKEWD KPEEFNPGRF 430 440 450 460 470 480 LDEQGQHIHS PSPSYLPFGA GIRVCLGEVL AKMELFLFLA WVLQRFTLEC PQDQPLPSLE 490 500 GKFGVVLQVQ KFRVKARLRE AWRGEMVR

References

[1]	"cDNA cloning and sequence analysis of a chicken gene expressed during the gonadal development and homologous to mammalian cytochrome P-450c17." Ono H., Iwasaki M., Sakamoto N., Mizuno S. Gene 66:77-85(1988) [PubMed: 3047010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M21406 mRNA. Translation: AAA48997.1.
IPI	IPI00602444.
PIR	O4CHC7. JT0318.
RefSeq	NP_001001901.1.
UniGene	Gga.834
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALG00000008121. Gallus gallus. [Contig view]
GeneID	425056.
KEGG	gga:425056.
Phylogenomic databases
HOVERGEN	P12394.
Enzyme and pathway databases
BRENDA	1.14.99.9. 4.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_CHICK

Accession

Primary (citable) accession number: P12394

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)