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Protein

Neuronal acetylcholine receptor subunit beta-4

Gene

Chrnb4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: RGD
  • acetylcholine binding Source: RGD
  • acetylcholine receptor activity Source: Ensembl
  • drug binding Source: RGD
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-RNO-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-RNO-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit beta-4
Alternative name(s):
Neuronal acetylcholine receptor non-alpha-2 chain
Short name:
N-alpha 2
Gene namesi
Name:Chrnb4
Synonyms:Acrb4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi2351. Chrnb4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 235215ExtracellularSequence analysisAdd
BLAST
Transmembranei236 – 25621HelicalSequence analysisAdd
BLAST
Topological domaini257 – 2648CytoplasmicSequence analysis
Transmembranei265 – 28521HelicalSequence analysisAdd
BLAST
Topological domaini286 – 29712ExtracellularSequence analysisAdd
BLAST
Transmembranei298 – 31821HelicalSequence analysisAdd
BLAST
Topological domaini319 – 463145CytoplasmicSequence analysisAdd
BLAST
Transmembranei464 – 48421HelicalSequence analysisAdd
BLAST
Topological domaini485 – 49511ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: RGD
  • cell junction Source: UniProtKB-KW
  • membrane Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: MGI
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3137276.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 495475Neuronal acetylcholine receptor subunit beta-4PRO_0000000391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence analysis
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence analysis
Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence analysis
Disulfide bondi152 ↔ 166By similarity
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP12392.

PTM databases

PhosphoSiteiP12392.

Expressioni

Tissue specificityi

In the brain, it is detected in the medial habenula. In the peripheral nervous system, it is found at least in the adrenal gland.

Gene expression databases

GenevisibleiP12392. RN.

Interactioni

Subunit structurei

Neuronal AChR is composed of two different types of subunits: alpha and beta. Beta-4 subunit can be combined to alpha-2, alpha-3 or alpha-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Chrna4P094834EBI-9008856,EBI-7842410

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiP12392. 4 interactions.
STRINGi10116.ENSRNOP00000051052.

Chemistry

BindingDBiP12392.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OLJmodel-B/D/E25-231[»]
ProteinModelPortaliP12392.
SMRiP12392. Positions 231-322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP12392.
KOiK04815.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Short (identifier: P12392-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGTPLLLVS LFSLLQDGDC RLANAEEKLM DDLLNKTRYN NLIRPATSSS
60 70 80 90 100
QLISIRLELS LSQLISVNER EQIMTTSIWL KQEWTDYRLA WNSSCYEGVN
110 120 130 140 150
ILRIPAKRVW LPDIVLYNNA DGTYEVSVYT NVIVRSNGSI QWLPPAIYKS
160 170 180 190 200
ACKIEVKHFP FDQQNCTLKF RSWTYDHTEI DMVLKSPTAI MDDFTPSGEW
210 220 230 240 250
DIVALPGRRT VNPQDPSYVD VTYDFIIKRK PLFYTINLII PCVLITSLAI
260 270 280 290 300
LVFYLPSDCG EKMTLCISVL LALTFFLLLI SKIVPPTSLD IPLIGKYLLF
310 320 330 340 350
TMVLVTFSIV TTVCVLNVHH RSPSTHTMAS WVKECFLHKL PTFLFMKRPG
360 370 380 390 400
LEVSLVRVPH PSQLHLATAD TAATSALGPT SPSNLYGSSM YFVNPVPAAP
410 420 430 440 450
KSAVSSHTAG LPRDARLRSS GRFREDLQEA LEGVSFIAQH LESDDRDQSV
460 470 480 490
IEDWKFVAMV VDRLFLWVFV FVCILGTMGL FLPPLFQIHA PSKDS
Length:495
Mass (Da):55,861
Last modified:October 1, 1989 - v1
Checksum:i688F37AB80448F7F
GO
Isoform Long (identifier: P12392-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     17-17: D → DARLFDCSGVLPDKGPAGLTVRFP

Show »
Length:518
Mass (Da):58,260
Checksum:i61946BD129580D29
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91V → VV in CAA33839 (PubMed:2918319).Curated
Sequence conflicti164 – 1641Missing in AAA41668 (PubMed:1689727).Curated
Sequence conflicti230 – 2312KP → NA in AAA41668 (PubMed:1689727).Curated
Sequence conflicti230 – 2312KP → NA in CAA33839 (PubMed:2918319).Curated
Sequence conflicti287 – 2882Missing in CAA33839 (PubMed:2918319).Curated
Sequence conflicti290 – 2901D → N in CAA33839 (PubMed:2918319).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei17 – 171D → DARLFDCSGVLPDKGPAGLT VRFP in isoform Long. 1 PublicationVSP_000076

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42976 mRNA. Translation: AAA85212.1.
M33953
, J05232, M89971, M33951, M89989, M33952 Genomic DNA. Translation: AAA41668.1.
X15834 mRNA. Translation: CAA33839.1.
AY574260 mRNA. Translation: AAS90356.1.
PIRiA30992.
B35721.
RefSeqiNP_434693.1. NM_052806.2. [P12392-1]
UniGeneiRn.9695.

Genome annotation databases

EnsembliENSRNOT00000019458; ENSRNOP00000019458; ENSRNOG00000014427. [P12392-1]
GeneIDi25103.
KEGGirno:25103.
UCSCiRGD:2351. rat. [P12392-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42976 mRNA. Translation: AAA85212.1.
M33953
, J05232, M89971, M33951, M89989, M33952 Genomic DNA. Translation: AAA41668.1.
X15834 mRNA. Translation: CAA33839.1.
AY574260 mRNA. Translation: AAS90356.1.
PIRiA30992.
B35721.
RefSeqiNP_434693.1. NM_052806.2. [P12392-1]
UniGeneiRn.9695.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OLJmodel-B/D/E25-231[»]
ProteinModelPortaliP12392.
SMRiP12392. Positions 231-322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP12392. 4 interactions.
STRINGi10116.ENSRNOP00000051052.

Chemistry

BindingDBiP12392.
ChEMBLiCHEMBL3137276.

PTM databases

PhosphoSiteiP12392.

Proteomic databases

PaxDbiP12392.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019458; ENSRNOP00000019458; ENSRNOG00000014427. [P12392-1]
GeneIDi25103.
KEGGirno:25103.
UCSCiRGD:2351. rat. [P12392-1]

Organism-specific databases

CTDi1143.
RGDi2351. Chrnb4.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP12392.
KOiK04815.

Enzyme and pathway databases

ReactomeiR-RNO-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-RNO-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-RNO-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

NextBioi605427.
PROiP12392.

Gene expression databases

GenevisibleiP12392. RN.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: beta 4."
    Duvoisin R.M., Deneris E.S., Patrick J., Heinemann S.F.
    Neuron 3:487-496(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
  2. "Alpha 3, alpha 5, and beta 4: three members of the rat neuronal nicotinic acetylcholine receptor-related gene family form a gene cluster."
    Boulter J., O'Shea-Greenfield A., Duvoisin R.M., Connolly J.G., Wada E., Jensen A., Gardner P.D., Ballivet M., Deneris E.S., McKinnon D., Heinemann S.F., Patrick J.
    J. Biol. Chem. 265:4472-4482(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
  3. "Cloning of a putative neuronal nicotinic acetylcholine receptor subunit."
    Isenberg K.E., Meyer G.E.
    J. Neurochem. 52:988-991(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Superior cervical ganglion.
  4. Groot-Kormelink P.J.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiACHB4_RAT
AccessioniPrimary (citable) accession number: P12392
Secondary accession number(s): Q63361
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 11, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.