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P12389

- ACHA2_RAT

UniProt

P12389 - ACHA2_RAT

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Protein
Neuronal acetylcholine receptor subunit alpha-2
Gene
Chrna2, Acra2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  1. acetylcholine binding Source: RGD
  2. acetylcholine receptor activity Source: Ensembl
  3. acetylcholine-activated cation-selective channel activity Source: RGD
  4. drug binding Source: RGD

GO - Biological processi

  1. cation transport Source: GOC
  2. protein heterooligomerization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_196477. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_196479. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-2
Gene namesi
Name:Chrna2
Synonyms:Acra2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 15

Organism-specific databases

RGDi621533. Chrna2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 241214Extracellular Reviewed prediction
Add
BLAST
Transmembranei242 – 26625Helical; Reviewed prediction
Add
BLAST
Transmembranei274 – 29219Helical; Reviewed prediction
Add
BLAST
Transmembranei308 – 32922Helical; Reviewed prediction
Add
BLAST
Topological domaini330 – 484155Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei485 – 50319Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. acetylcholine-gated channel complex Source: RGD
  2. cell junction Source: UniProtKB-KW
  3. membrane Source: RGD
  4. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction
Add
BLAST
Chaini28 – 511484Neuronal acetylcholine receptor subunit alpha-2
PRO_0000000343Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi106 – 1061N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi160 ↔ 174 By similarity
Glycosylationi212 – 2121N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi224 ↔ 225Associated with receptor activation By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP12389.

PTM databases

PhosphoSiteiP12389.

Expressioni

Gene expression databases

GenevestigatoriP12389.

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023475.

Structurei

3D structure databases

ProteinModelPortaliP12389.
SMRiP12389. Positions 242-333.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi391 – 40212Poly-Glu
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290206.
GeneTreeiENSGT00750000117277.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP12389.
KOiK04804.
OMAiACAGHVA.
OrthoDBiEOG72JWGV.
PhylomeDBiP12389.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12389-1 [UniParc]FASTAAdd to Basket

« Hide

MTLSHSALQF WTHLYLWCLL LVPAVLTQQG SHTHAEDRLF KHLFGGYNRW    50
ARPVPNTSDV VIVRFGLSIA QLIDVDEKNQ MMTTNVWLKQ EWNDYKLRWD 100
PAEFGNVTSL RVPSEMIWIP DIVLYNNADG EFAVTHMTKA HLFFTGTVHW 150
VPPAIYKSSC SIDVTFFPFD QQNCKMKFGS WTYDKAKIDL EQMERTVDLK 200
DYWESGEWAI INATGTYNSK KYDCCAEIYP DVTYYFVIRR LPLFYTINLI 250
IPCLLISCLT VLVFYLPSEC GEKITLCISV LLSLTVFLLL ITEIIPSTSL 300
VIPLIGEYLL FTMIFVTLSI VITVFVLNVH HRSPSTHNMP NWVRVALLGR 350
VPRWLMMNRP LPPMELHGSP DLKLSPSYHW LETNMDAGER EETEEEEEEE 400
DENICVCAGL PDSSMGVLYG HGGLHLRAME PETKTPSQAS EILLSPQIQK 450
ALEGVHYIAD RLRSEDADSS VKEDWKYVAM VVDRIFLWLF IIVCFLGTIG 500
LFLPPFLAGM I 511
Length:511
Mass (Da):58,611
Last modified:July 15, 1998 - v2
Checksum:i3824E83BB01D613B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti494 – 4941C → S in AAA40664. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10077 mRNA. Translation: AAB60900.1.
M20297
, M20292, M20293, M20294, M20295, M20296 Genomic DNA. Translation: AAA40664.1.
AY574253 mRNA. Translation: AAS90349.1.
PIRiA40110.
RefSeqiNP_596911.1. NM_133420.1.
UniGeneiRn.9713.

Genome annotation databases

EnsembliENSRNOT00000023475; ENSRNOP00000023475; ENSRNOG00000017424.
GeneIDi170945.
KEGGirno:170945.
UCSCiRGD:621533. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L10077 mRNA. Translation: AAB60900.1 .
M20297
, M20292 , M20293 , M20294 , M20295 , M20296 Genomic DNA. Translation: AAA40664.1 .
AY574253 mRNA. Translation: AAS90349.1 .
PIRi A40110.
RefSeqi NP_596911.1. NM_133420.1.
UniGenei Rn.9713.

3D structure databases

ProteinModelPortali P12389.
SMRi P12389. Positions 242-333.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000023475.

Chemistry

BindingDBi P12389.
ChEMBLi CHEMBL1907590.
GuidetoPHARMACOLOGYi 463.

PTM databases

PhosphoSitei P12389.

Proteomic databases

PaxDbi P12389.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000023475 ; ENSRNOP00000023475 ; ENSRNOG00000017424 .
GeneIDi 170945.
KEGGi rno:170945.
UCSCi RGD:621533. rat.

Organism-specific databases

CTDi 1135.
RGDi 621533. Chrna2.

Phylogenomic databases

eggNOGi NOG290206.
GeneTreei ENSGT00750000117277.
HOGENOMi HOG000006756.
HOVERGENi HBG003756.
InParanoidi P12389.
KOi K04804.
OMAi ACAGHVA.
OrthoDBi EOG72JWGV.
PhylomeDBi P12389.
TreeFami TF315605.

Enzyme and pathway databases

Reactomei REACT_196477. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_196479. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

NextBioi 621469.
PROi P12389.

Gene expression databases

Genevestigatori P12389.

Family and domain databases

Gene3Di 1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProi IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view ]
PANTHERi PTHR18945. PTHR18945. 1 hit.
Pfami PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view ]
PRINTSi PR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMi SSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsi TIGR00860. LIC. 1 hit.
PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor."
    Wada K., Ballivet M., Boulter J., Connolly J.G., Wada E., Deneris E.S., Swanson L.W., Heinemann S.F., Patrick J.
    Science 240:330-334(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Boulter J.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Groot-Kormelink P.J.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiACHA2_RAT
AccessioniPrimary (citable) accession number: P12389
Secondary accession number(s): O08952, Q53YK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: September 3, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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