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P12389 (ACHA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit alpha-2
Gene names
Name:Chrna2
Synonyms:Acra2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length511 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure

Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-2/CHRNA2 sub-subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 511484Neuronal acetylcholine receptor subunit alpha-2
PRO_0000000343

Regions

Topological domain28 – 241214Extracellular Potential
Transmembrane242 – 26625Helical; Potential
Transmembrane274 – 29219Helical; Potential
Transmembrane308 – 32922Helical; Potential
Topological domain330 – 484155Cytoplasmic Potential
Transmembrane485 – 50319Helical; Potential
Compositional bias391 – 40212Poly-Glu

Amino acid modifications

Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation2121N-linked (GlcNAc...) Potential
Disulfide bond160 ↔ 174 By similarity
Disulfide bond224 ↔ 225Associated with receptor activation By similarity

Experimental info

Sequence conflict4941C → S in AAA40664. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P12389 [UniParc].

Last modified July 15, 1998. Version 2.
Checksum: 3824E83BB01D613B

FASTA51158,611
        10         20         30         40         50         60 
MTLSHSALQF WTHLYLWCLL LVPAVLTQQG SHTHAEDRLF KHLFGGYNRW ARPVPNTSDV 

        70         80         90        100        110        120 
VIVRFGLSIA QLIDVDEKNQ MMTTNVWLKQ EWNDYKLRWD PAEFGNVTSL RVPSEMIWIP 

       130        140        150        160        170        180 
DIVLYNNADG EFAVTHMTKA HLFFTGTVHW VPPAIYKSSC SIDVTFFPFD QQNCKMKFGS 

       190        200        210        220        230        240 
WTYDKAKIDL EQMERTVDLK DYWESGEWAI INATGTYNSK KYDCCAEIYP DVTYYFVIRR 

       250        260        270        280        290        300 
LPLFYTINLI IPCLLISCLT VLVFYLPSEC GEKITLCISV LLSLTVFLLL ITEIIPSTSL 

       310        320        330        340        350        360 
VIPLIGEYLL FTMIFVTLSI VITVFVLNVH HRSPSTHNMP NWVRVALLGR VPRWLMMNRP 

       370        380        390        400        410        420 
LPPMELHGSP DLKLSPSYHW LETNMDAGER EETEEEEEEE DENICVCAGL PDSSMGVLYG 

       430        440        450        460        470        480 
HGGLHLRAME PETKTPSQAS EILLSPQIQK ALEGVHYIAD RLRSEDADSS VKEDWKYVAM 

       490        500        510 
VVDRIFLWLF IIVCFLGTIG LFLPPFLAGM I 

« Hide

References

[1]"Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor."
Wada K., Ballivet M., Boulter J., Connolly J.G., Wada E., Deneris E.S., Swanson L.W., Heinemann S.F., Patrick J.
Science 240:330-334(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]Boulter J.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]Groot-Kormelink P.J.
Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L10077 mRNA. Translation: AAB60900.1.
M20297 expand/collapse EMBL AC list , M20292, M20293, M20294, M20295, M20296 Genomic DNA. Translation: AAA40664.1.
AY574253 mRNA. Translation: AAS90349.1.
PIRA40110.
RefSeqNP_596911.1. NM_133420.1.
UniGeneRn.9713.

3D structure databases

ProteinModelPortalP12389.
SMRP12389. Positions 242-333.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000023475.

Chemistry

BindingDBP12389.
ChEMBLCHEMBL1907590.
GuidetoPHARMACOLOGY463.

PTM databases

PhosphoSiteP12389.

Proteomic databases

PaxDbP12389.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000023475; ENSRNOP00000023475; ENSRNOG00000017424.
GeneID170945.
KEGGrno:170945.
UCSCRGD:621533. rat.

Organism-specific databases

CTD1135.
RGD621533. Chrna2.

Phylogenomic databases

eggNOGNOG290206.
GeneTreeENSGT00750000117277.
HOGENOMHOG000006756.
HOVERGENHBG003756.
InParanoidP12389.
KOK04804.
OMAACAGHVA.
OrthoDBEOG72JWGV.
PhylomeDBP12389.
TreeFamTF315605.

Gene expression databases

GenevestigatorP12389.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio621469.
PROP12389.

Entry information

Entry nameACHA2_RAT
AccessionPrimary (citable) accession number: P12389
Secondary accession number(s): O08952, Q53YK3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: June 11, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families