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Protein

Neuronal acetylcholine receptor subunit alpha-2

Gene

Chrna2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  1. acetylcholine-activated cation-selective channel activity Source: RGD
  2. acetylcholine binding Source: RGD
  3. acetylcholine receptor activity Source: Ensembl
  4. drug binding Source: RGD

GO - Biological processi

  1. cation transmembrane transport Source: GOC
  2. protein heterooligomerization Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_345035. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_353330. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-2
Gene namesi
Name:Chrna2
Synonyms:Acra2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi621533. Chrna2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 241214ExtracellularSequence AnalysisAdd
BLAST
Transmembranei242 – 26625HelicalSequence AnalysisAdd
BLAST
Transmembranei274 – 29219HelicalSequence AnalysisAdd
BLAST
Transmembranei308 – 32922HelicalSequence AnalysisAdd
BLAST
Topological domaini330 – 484155CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei485 – 50319HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. acetylcholine-gated channel complex Source: RGD
  2. cell junction Source: UniProtKB-KW
  3. membrane Source: RGD
  4. postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Chaini28 – 511484Neuronal acetylcholine receptor subunit alpha-2PRO_0000000343Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi160 ↔ 174By similarity
Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi224 ↔ 225Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP12389.

PTM databases

PhosphoSiteiP12389.

Expressioni

Gene expression databases

GenevestigatoriP12389.

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023475.

Structurei

3D structure databases

ProteinModelPortaliP12389.
SMRiP12389. Positions 242-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi391 – 40212Poly-GluAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290206.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP12389.
KOiK04804.
OMAiACAGHVA.
OrthoDBiEOG72JWGV.
PhylomeDBiP12389.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12389-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSHSALQF WTHLYLWCLL LVPAVLTQQG SHTHAEDRLF KHLFGGYNRW
60 70 80 90 100
ARPVPNTSDV VIVRFGLSIA QLIDVDEKNQ MMTTNVWLKQ EWNDYKLRWD
110 120 130 140 150
PAEFGNVTSL RVPSEMIWIP DIVLYNNADG EFAVTHMTKA HLFFTGTVHW
160 170 180 190 200
VPPAIYKSSC SIDVTFFPFD QQNCKMKFGS WTYDKAKIDL EQMERTVDLK
210 220 230 240 250
DYWESGEWAI INATGTYNSK KYDCCAEIYP DVTYYFVIRR LPLFYTINLI
260 270 280 290 300
IPCLLISCLT VLVFYLPSEC GEKITLCISV LLSLTVFLLL ITEIIPSTSL
310 320 330 340 350
VIPLIGEYLL FTMIFVTLSI VITVFVLNVH HRSPSTHNMP NWVRVALLGR
360 370 380 390 400
VPRWLMMNRP LPPMELHGSP DLKLSPSYHW LETNMDAGER EETEEEEEEE
410 420 430 440 450
DENICVCAGL PDSSMGVLYG HGGLHLRAME PETKTPSQAS EILLSPQIQK
460 470 480 490 500
ALEGVHYIAD RLRSEDADSS VKEDWKYVAM VVDRIFLWLF IIVCFLGTIG
510
LFLPPFLAGM I
Length:511
Mass (Da):58,611
Last modified:July 15, 1998 - v2
Checksum:i3824E83BB01D613B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti494 – 4941C → S in AAA40664 (PubMed:2832952).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10077 mRNA. Translation: AAB60900.1.
M20297
, M20292, M20293, M20294, M20295, M20296 Genomic DNA. Translation: AAA40664.1.
AY574253 mRNA. Translation: AAS90349.1.
PIRiA40110.
RefSeqiNP_596911.1. NM_133420.1.
UniGeneiRn.9713.

Genome annotation databases

EnsembliENSRNOT00000023475; ENSRNOP00000023475; ENSRNOG00000017424.
GeneIDi170945.
KEGGirno:170945.
UCSCiRGD:621533. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10077 mRNA. Translation: AAB60900.1.
M20297
, M20292, M20293, M20294, M20295, M20296 Genomic DNA. Translation: AAA40664.1.
AY574253 mRNA. Translation: AAS90349.1.
PIRiA40110.
RefSeqiNP_596911.1. NM_133420.1.
UniGeneiRn.9713.

3D structure databases

ProteinModelPortaliP12389.
SMRiP12389. Positions 242-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023475.

Chemistry

BindingDBiP12389.
ChEMBLiCHEMBL2094110.
GuidetoPHARMACOLOGYi463.

PTM databases

PhosphoSiteiP12389.

Proteomic databases

PaxDbiP12389.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023475; ENSRNOP00000023475; ENSRNOG00000017424.
GeneIDi170945.
KEGGirno:170945.
UCSCiRGD:621533. rat.

Organism-specific databases

CTDi1135.
RGDi621533. Chrna2.

Phylogenomic databases

eggNOGiNOG290206.
GeneTreeiENSGT00760000118930.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP12389.
KOiK04804.
OMAiACAGHVA.
OrthoDBiEOG72JWGV.
PhylomeDBiP12389.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiREACT_345035. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
REACT_353330. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

NextBioi621469.
PROiP12389.

Gene expression databases

GenevestigatoriP12389.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor."
    Wada K., Ballivet M., Boulter J., Connolly J.G., Wada E., Deneris E.S., Swanson L.W., Heinemann S.F., Patrick J.
    Science 240:330-334(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Boulter J.
    Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Groot-Kormelink P.J.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiACHA2_RAT
AccessioniPrimary (citable) accession number: P12389
Secondary accession number(s): O08952, Q53YK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 15, 1998
Last modified: April 1, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.