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P12389

- ACHA2_RAT

UniProt

P12389 - ACHA2_RAT

Protein

Neuronal acetylcholine receptor subunit alpha-2

Gene

Chrna2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

    GO - Molecular functioni

    1. acetylcholine-activated cation-selective channel activity Source: RGD
    2. acetylcholine binding Source: RGD
    3. acetylcholine receptor activity Source: Ensembl
    4. drug binding Source: RGD

    GO - Biological processi

    1. cation transport Source: GOC
    2. protein heterooligomerization Source: RGD

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196477. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_196479. Highly calcium permeable nicotinic acetylcholine receptors.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuronal acetylcholine receptor subunit alpha-2
    Gene namesi
    Name:Chrna2
    Synonyms:Acra2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 15

    Organism-specific databases

    RGDi621533. Chrna2.

    Subcellular locationi

    GO - Cellular componenti

    1. acetylcholine-gated channel complex Source: RGD
    2. cell junction Source: UniProtKB-KW
    3. membrane Source: RGD
    4. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 511484Neuronal acetylcholine receptor subunit alpha-2PRO_0000000343Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi106 – 1061N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi160 ↔ 174By similarity
    Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi224 ↔ 225Associated with receptor activationBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP12389.

    PTM databases

    PhosphoSiteiP12389.

    Expressioni

    Gene expression databases

    GenevestigatoriP12389.

    Interactioni

    Subunit structurei

    Neuronal AChR seems to be composed of two different types of subunits: alpha and non-alpha (beta). Alpha-2 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000023475.

    Structurei

    3D structure databases

    ProteinModelPortaliP12389.
    SMRiP12389. Positions 242-333.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 241214ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini330 – 484155CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei242 – 26625HelicalSequence AnalysisAdd
    BLAST
    Transmembranei274 – 29219HelicalSequence AnalysisAdd
    BLAST
    Transmembranei308 – 32922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei485 – 50319HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi391 – 40212Poly-GluAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG290206.
    GeneTreeiENSGT00750000117277.
    HOGENOMiHOG000006756.
    HOVERGENiHBG003756.
    InParanoidiP12389.
    KOiK04804.
    OMAiACAGHVA.
    OrthoDBiEOG72JWGV.
    PhylomeDBiP12389.
    TreeFamiTF315605.

    Family and domain databases

    Gene3Di1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProiIPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 2 hits.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12389-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLSHSALQF WTHLYLWCLL LVPAVLTQQG SHTHAEDRLF KHLFGGYNRW    50
    ARPVPNTSDV VIVRFGLSIA QLIDVDEKNQ MMTTNVWLKQ EWNDYKLRWD 100
    PAEFGNVTSL RVPSEMIWIP DIVLYNNADG EFAVTHMTKA HLFFTGTVHW 150
    VPPAIYKSSC SIDVTFFPFD QQNCKMKFGS WTYDKAKIDL EQMERTVDLK 200
    DYWESGEWAI INATGTYNSK KYDCCAEIYP DVTYYFVIRR LPLFYTINLI 250
    IPCLLISCLT VLVFYLPSEC GEKITLCISV LLSLTVFLLL ITEIIPSTSL 300
    VIPLIGEYLL FTMIFVTLSI VITVFVLNVH HRSPSTHNMP NWVRVALLGR 350
    VPRWLMMNRP LPPMELHGSP DLKLSPSYHW LETNMDAGER EETEEEEEEE 400
    DENICVCAGL PDSSMGVLYG HGGLHLRAME PETKTPSQAS EILLSPQIQK 450
    ALEGVHYIAD RLRSEDADSS VKEDWKYVAM VVDRIFLWLF IIVCFLGTIG 500
    LFLPPFLAGM I 511
    Length:511
    Mass (Da):58,611
    Last modified:July 15, 1998 - v2
    Checksum:i3824E83BB01D613B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti494 – 4941C → S in AAA40664. (PubMed:2832952)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10077 mRNA. Translation: AAB60900.1.
    M20297
    , M20292, M20293, M20294, M20295, M20296 Genomic DNA. Translation: AAA40664.1.
    AY574253 mRNA. Translation: AAS90349.1.
    PIRiA40110.
    RefSeqiNP_596911.1. NM_133420.1.
    UniGeneiRn.9713.

    Genome annotation databases

    EnsembliENSRNOT00000023475; ENSRNOP00000023475; ENSRNOG00000017424.
    GeneIDi170945.
    KEGGirno:170945.
    UCSCiRGD:621533. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L10077 mRNA. Translation: AAB60900.1 .
    M20297
    , M20292 , M20293 , M20294 , M20295 , M20296 Genomic DNA. Translation: AAA40664.1 .
    AY574253 mRNA. Translation: AAS90349.1 .
    PIRi A40110.
    RefSeqi NP_596911.1. NM_133420.1.
    UniGenei Rn.9713.

    3D structure databases

    ProteinModelPortali P12389.
    SMRi P12389. Positions 242-333.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000023475.

    Chemistry

    BindingDBi P12389.
    ChEMBLi CHEMBL1907590.
    GuidetoPHARMACOLOGYi 463.

    PTM databases

    PhosphoSitei P12389.

    Proteomic databases

    PaxDbi P12389.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023475 ; ENSRNOP00000023475 ; ENSRNOG00000017424 .
    GeneIDi 170945.
    KEGGi rno:170945.
    UCSCi RGD:621533. rat.

    Organism-specific databases

    CTDi 1135.
    RGDi 621533. Chrna2.

    Phylogenomic databases

    eggNOGi NOG290206.
    GeneTreei ENSGT00750000117277.
    HOGENOMi HOG000006756.
    HOVERGENi HBG003756.
    InParanoidi P12389.
    KOi K04804.
    OMAi ACAGHVA.
    OrthoDBi EOG72JWGV.
    PhylomeDBi P12389.
    TreeFami TF315605.

    Enzyme and pathway databases

    Reactomei REACT_196477. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
    REACT_196479. Highly calcium permeable nicotinic acetylcholine receptors.

    Miscellaneous databases

    NextBioi 621469.
    PROi P12389.

    Gene expression databases

    Genevestigatori P12389.

    Family and domain databases

    Gene3Di 1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProi IPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    IPR002394. Nicotinic_acetylcholine_rcpt.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR00254. NICOTINICR.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 2 hits.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Functional expression of a new pharmacological subtype of brain nicotinic acetylcholine receptor."
      Wada K., Ballivet M., Boulter J., Connolly J.G., Wada E., Deneris E.S., Swanson L.W., Heinemann S.F., Patrick J.
      Science 240:330-334(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. Boulter J.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. Groot-Kormelink P.J.
      Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiACHA2_RAT
    AccessioniPrimary (citable) accession number: P12389
    Secondary accession number(s): O08952, Q53YK3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3