P12388 (PAI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plasminogen activator inhibitor 2, macrophage
Short name=PAI-2

Alternative name(s):
Serpin B2

Gene names

Name:	Serpinb2
Synonyms:	Pai2, Planh2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1. Not required for normal murine development or survival.
Subunit structure	Interacts with PSMB1 By similarity.
Subcellular location	Cytoplasm. Secreted › extracellular space.
Post-translational modification	The signal sequence is not cleaved.
Sequence similarities	Belongs to the serpin family. Ov-serpin subfamily.

Ontologies

Keywords
Biological process	Plasminogen activation
Cellular component	Cytoplasm Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of proteolysis Inferred from Biological aspect of Ancestor. Source: RefGenome wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Plasminogen activator inhibitor 2, macrophage		PRO_0000223297
	Signal peptide	1 – ?		Not cleaved
Sites
	Site	380 – 381	2	Reactive bond
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	261	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	239	1	A → E. Ref.3
	Natural variant	258	1	H → Y. Ref.3
	Natural variant	259	1	T → I. Ref.3
	Natural variant	284	1	S → R. Ref.3
Experimental info
	Sequence conflict	55	1	A → T Ref.3
	Sequence conflict	55	1	A → T Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P12388 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: C4FC78C5C3CA7D8B	FASTA	415	46,292
	10 20 30 40 50 60 MEELSMANTM FALNLLKQIE KSNSTQNIFI SPWSISSTLA IVLLGAGGNT EQQMAKVLQF 70 80 90 100 110 120 NEIGSYGITT RNPENFSGCD FAQQIQKENY PSAILQAQAG DKIHSAFSSL SSTINTPQGD 130 140 150 160 170 180 YLLESANKLF GEKSARFKEE YIQLSKKYYS TEPEAVDFLE CAEEAREKIN SWVKTQTKGE 190 200 210 220 230 240 IPNLLPEGSV DEDTKMVLVN AVYFKGKWKT PFEKKLNGLY PFRVNSHESI PVQMMFLHAK 250 260 270 280 290 300 LNIGYIKDLK TQILELPHTG NISMLLLLPD EIEDASTGLE LLESEINFAN FNKWISKDTL 310 320 330 340 350 360 DEDDVVVYIP KFKLAQSYEL KSILQSMGME DAFNKGKANF SGMSERNDLF LSEVFHQASV 370 380 390 400 410 DVTEEGTVAA GGTGAVMTGR TGHGGPQFVA DHPFLFFIMD KITHTILFVG RFSSP

References

[1]	"Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2." Belin D., Wohlwend A., Schleuning W.-D., Kruithof E.K.O., Vassalli J.-D. EMBO J. 8:3287-3294(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-27. Strain: AKR. Tissue: Myeloid.
[2]	Belin D. Submitted (SEP-1989) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AKR. Tissue: Myeloid.
[3]	"Sequence and tissue-specific expression of the murine PAI-2 gene." Belin D., Tapparel C., Sappino N., Silva F. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS GLU-239; TYR-258; ILE-259 AND ARG-284. Strain: 129. Tissue: Liver.
[4]	"The plasminogen activator inhibitor-2 gene is not required for normal murine development or survival." Dougherty K.M., Pearson J.M., Yang A.Y., Westrick R.J., Baker M.S., Ginsburg D. Proc. Natl. Acad. Sci. U.S.A. 96:686-691(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34; 54-74; 89-115; 124-156; 163-237 AND 265-302. Strain: 129/Sv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16490 mRNA. Translation: CAA34507.1. AJ000386, AJ000384, AJ000385 Genomic DNA. Translation: CAA04047.1. AF069683 Genomic DNA. Translation: AAD08915.1. AF069685, AF069684 Genomic DNA. Translation: AAD08916.1. AF069687, AF069686 Genomic DNA. Translation: AAD08917.1. AF069689, AF069688 Genomic DNA. Translation: AAD08918.1. AF069690 Genomic DNA. Translation: AAD08919.1. AF069692, AF069691 Genomic DNA. Translation: AAD08920.1. AF069694, AF069693 Genomic DNA. Translation: AAD08921.1.
IPI	IPI00116606.
PIR	S20047.
RefSeq	NP_001167641.1. NM_001174170.1. NP_035241.1. NM_011111.4.
UniGene	Mm.271870.
3D structure databases
ProteinModelPortal	P12388.
SMR	P12388. Positions 3-415.
ModBase	Search...
Protein family/group databases
MEROPS	I04.007.
PTM databases
PhosphoSite	P12388.
Proteomic databases
PaxDb	P12388.
PRIDE	P12388.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000009356; ENSMUSP00000009356; ENSMUSG00000062345. ENSMUST00000064916; ENSMUSP00000065277; ENSMUSG00000062345.
GeneID	18788.
KEGG	mmu:18788.
UCSC	uc007chn.2. mouse.
Organism-specific databases
CTD	5055.
MGI	MGI:97609. Serpinb2.
Phylogenomic databases
eggNOG	COG4826.
HOVERGEN	HBG005957.
InParanoid	P12388.
KO	K13963.
OMA	LFLIMHK.
OrthoDB	EOG4PC9S5.
Gene expression databases
ArrayExpress	P12388.
Bgee	P12388.
Genevestigator	P12388.
GermOnline	ENSMUSG00000062345. Mus musculus.
Family and domain databases
InterPro	IPR023795. Protease_inhib_I4_serpin_CS. IPR015556. Protease_inhib_serine_PAI-2. IPR023796. Serpin_dom. IPR000215. Serpin_fam. [Graphical view]
PANTHER	PTHR11461. PTHR11461. 1 hit. PTHR11461:SF61. PTHR11461:SF61. 1 hit.
Pfam	PF00079. Serpin. 1 hit. [Graphical view]
SMART	SM00093. SERPIN. 1 hit. [Graphical view]
SUPFAM	SSF56574. Prot_inh_serpin. 1 hit.
PROSITE	PS00284. SERPIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SERPINB2. mouse.
NextBio	295060.
SOURCE	Search...

Entry information

Entry name

PAI2_MOUSE

Accession

Primary (citable) accession number: P12388
Secondary accession number(s): O35687 , Q9QWP6, Q9QWP7, Q9QWP8, Q9QWP9, Q9QWQ0, Q9QWZ5, Q9QWZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	May 29, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program