Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P12378

- UGDH_BOVIN

UniProt

P12378 - UGDH_BOVIN

Protein

UDP-glucose 6-dehydrogenase

Gene

UGDH

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

    Catalytic activityi

    UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

    Enzyme regulationi

    UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361NADBy similarity
    Binding sitei41 – 411NADBy similarity
    Binding sitei165 – 1651NADBy similarity
    Active sitei276 – 2761NucleophileBy similarity
    Binding sitei346 – 3461NADBy similarity
    Binding sitei442 – 4421SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 166NADBy similarity
    Nucleotide bindingi89 – 935NADBy similarity
    Nucleotide bindingi130 – 1312NADBy similarity
    Nucleotide bindingi276 – 2794NADBy similarity

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. UDP-glucose 6-dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. gastrulation with mouth forming second Source: AgBase
    2. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    ReactomeiREACT_214113. Formation of the active cofactor, UDP-glucuronate.
    UniPathwayiUPA00038; UER00491.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
    Short name:
    UDP-Glc dehydrogenase
    Short name:
    UDP-GlcDH
    Short name:
    UDPGDH
    Gene namesi
    Name:UGDH
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 6

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494UDP-glucose 6-dehydrogenasePRO_0000074059Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei107 – 1071N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP12378.
    PRIDEiP12378.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP12378.
    SMRiP12378. Positions 1-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni161 – 1655Substrate bindingBy similarity
    Regioni220 – 2278Substrate bindingBy similarity
    Regioni260 – 27314Substrate bindingBy similarityAdd
    BLAST
    Regioni338 – 3392Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1004.
    GeneTreeiENSGT00390000015355.
    HOGENOMiHOG000153773.
    HOVERGENiHBG003512.
    InParanoidiP12378.
    KOiK00012.
    OMAiAIGRSTQ.
    OrthoDBiEOG7034GP.
    TreeFamiTF105671.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028356. UDPglc_DH_euk.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PTHR11374:SF3. PTHR11374:SF3. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P12378-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDINES RINAWNSPTL    50
    PIYEPGLKEV VESCRGKNLF FSTNIDDAIK EADLVFISVN TPTKTYGMGK 100
    GRAADLKYIE ACARRIVQNS HGYKIVTEKS TVPVRAAESI RRIFDANTKP 150
    NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI GGDETPEGQR AVQALCAVYE 200
    HWVPREKILT TNTWSSELSK LTANAFLAQR ISSINSISAL CEATGADVEE 250
    VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW 300
    QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS 350
    IYISKYLMDE GAHLHIYDPK VPREQIVVDL SHPGVSKDDQ VARLVTISKD 400
    PYEACDGAHA VVICTEWDMF KELDYERIHK KMLKPAFIFD GRRVLDGLHN 450
    ELQTIGFQIE TIGKKVSSKR IPYAPSGEIP KFSLQDMPNK KPRV 494
    Length:494
    Mass (Da):55,136
    Last modified:December 15, 1998 - v2
    Checksum:i8560E27089C4D03E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti157 – 1571Missing AA sequence (PubMed:7920253)Curated
    Sequence conflicti278 – 2814QKDV → ZZGK AA sequence (PubMed:6896145)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095792 mRNA. Translation: AAC64183.1.
    BC150068 mRNA. Translation: AAI50069.1.
    PIRiA17150.
    RefSeqiNP_776636.1. NM_174211.3.
    XP_005207890.1. XM_005207833.1.
    UniGeneiBt.5538.

    Genome annotation databases

    EnsembliENSBTAT00000019302; ENSBTAP00000019302; ENSBTAG00000014521.
    GeneIDi281564.
    KEGGibta:281564.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF095792 mRNA. Translation: AAC64183.1 .
    BC150068 mRNA. Translation: AAI50069.1 .
    PIRi A17150.
    RefSeqi NP_776636.1. NM_174211.3.
    XP_005207890.1. XM_005207833.1.
    UniGenei Bt.5538.

    3D structure databases

    ProteinModelPortali P12378.
    SMRi P12378. Positions 1-466.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P12378.
    PRIDEi P12378.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000019302 ; ENSBTAP00000019302 ; ENSBTAG00000014521 .
    GeneIDi 281564.
    KEGGi bta:281564.

    Organism-specific databases

    CTDi 7358.

    Phylogenomic databases

    eggNOGi COG1004.
    GeneTreei ENSGT00390000015355.
    HOGENOMi HOG000153773.
    HOVERGENi HBG003512.
    InParanoidi P12378.
    KOi K00012.
    OMAi AIGRSTQ.
    OrthoDBi EOG7034GP.
    TreeFami TF105671.

    Enzyme and pathway databases

    UniPathwayi UPA00038 ; UER00491 .
    Reactomei REACT_214113. Formation of the active cofactor, UDP-glucuronate.

    Miscellaneous databases

    NextBioi 20805514.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028356. UDPglc_DH_euk.
    [Graphical view ]
    PANTHERi PTHR11374. PTHR11374. 1 hit.
    PTHR11374:SF3. PTHR11374:SF3. 1 hit.
    Pfami PF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500133. UDPglc_DH_euk. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of UDP-glucose dehydrogenase from bovine kidney."
      Lind T., Falk E., Hjertson E., Kusche-Gullberg M., Lidholt K.
      Glycobiology 9:595-600(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Ascending colon.
    3. "UDP-glucose dehydrogenase from bovine liver: primary structure and relationship to other dehydrogenases."
      Hempel J., Perozich J., Romovacek H., Hinich A., Kuo I., Feingold D.S.
      Protein Sci. 3:1074-1080(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-469, SUBUNIT.
      Tissue: Liver.
    4. "Amino acid sequence of the tryptic peptide containing the catalytic-site thiol group of bovine liver uridine diphosphate glucose dehydrogenase."
      Franzen B., Carrubba C., Feingold D.S., Ashcom J., Franzen J.S.
      Biochem. J. 199:599-602(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 268-281.
      Tissue: Liver.

    Entry informationi

    Entry nameiUGDH_BOVIN
    AccessioniPrimary (citable) accession number: P12378
    Secondary accession number(s): A6QR10, O77806
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3