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Protein

UDP-glucose 6-dehydrogenase

Gene

UGDH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

Enzyme regulationi

UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch.By similarity

Pathwayi: UDP-alpha-D-glucuronate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose.
Proteins known to be involved in this subpathway in this organism are:
  1. UDP-glucose 6-dehydrogenase (UGDH)
This subpathway is part of the pathway UDP-alpha-D-glucuronate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose, the pathway UDP-alpha-D-glucuronate biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NADBy similarity1
Binding sitei41NADBy similarity1
Binding sitei165NADBy similarity1
Binding sitei260SubstrateBy similarity1
Active sitei276NucleophileBy similarity1
Binding sitei346NADBy similarity1
Binding sitei442SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 16NADBy similarity6
Nucleotide bindingi89 – 93NADBy similarity5
Nucleotide bindingi130 – 131NADBy similarity2
Nucleotide bindingi276 – 279NADBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-BTA-173599. Formation of the active cofactor, UDP-glucuronate.
UniPathwayiUPA00038; UER00491.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
Short name:
UDP-Glc dehydrogenase
Short name:
UDP-GlcDH
Short name:
UDPGDH
Gene namesi
Name:UGDH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000740591 – 494UDP-glucose 6-dehydrogenaseAdd BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei107N6-acetyllysineBy similarity1
Modified residuei476PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP12378.
PeptideAtlasiP12378.
PRIDEiP12378.

Expressioni

Gene expression databases

BgeeiENSBTAG00000014521.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019302.

Structurei

3D structure databases

ProteinModelPortaliP12378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 165Substrate bindingBy similarity5
Regioni220 – 224Substrate bindingBy similarity5
Regioni267 – 273Substrate bindingBy similarity7
Regioni338 – 339Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2666. Eukaryota.
COG1004. LUCA.
GeneTreeiENSGT00390000015355.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiP12378.
KOiK00012.
OMAiVIIMNDY.
OrthoDBiEOG091G05RK.
TreeFamiTF105671.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

P12378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDINES RINAWNSPTL
60 70 80 90 100
PIYEPGLKEV VESCRGKNLF FSTNIDDAIK EADLVFISVN TPTKTYGMGK
110 120 130 140 150
GRAADLKYIE ACARRIVQNS HGYKIVTEKS TVPVRAAESI RRIFDANTKP
160 170 180 190 200
NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI GGDETPEGQR AVQALCAVYE
210 220 230 240 250
HWVPREKILT TNTWSSELSK LTANAFLAQR ISSINSISAL CEATGADVEE
260 270 280 290 300
VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW
310 320 330 340 350
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS
360 370 380 390 400
IYISKYLMDE GAHLHIYDPK VPREQIVVDL SHPGVSKDDQ VARLVTISKD
410 420 430 440 450
PYEACDGAHA VVICTEWDMF KELDYERIHK KMLKPAFIFD GRRVLDGLHN
460 470 480 490
ELQTIGFQIE TIGKKVSSKR IPYAPSGEIP KFSLQDMPNK KPRV
Length:494
Mass (Da):55,136
Last modified:December 15, 1998 - v2
Checksum:i8560E27089C4D03E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157Missing AA sequence (PubMed:7920253).Curated1
Sequence conflicti278 – 281QKDV → ZZGK AA sequence (PubMed:6896145).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095792 mRNA. Translation: AAC64183.1.
BC150068 mRNA. Translation: AAI50069.1.
PIRiA17150.
RefSeqiNP_776636.1. NM_174211.3.
XP_005207890.1. XM_005207833.2.
UniGeneiBt.5538.

Genome annotation databases

EnsembliENSBTAT00000019302; ENSBTAP00000019302; ENSBTAG00000014521.
GeneIDi281564.
KEGGibta:281564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095792 mRNA. Translation: AAC64183.1.
BC150068 mRNA. Translation: AAI50069.1.
PIRiA17150.
RefSeqiNP_776636.1. NM_174211.3.
XP_005207890.1. XM_005207833.2.
UniGeneiBt.5538.

3D structure databases

ProteinModelPortaliP12378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019302.

Proteomic databases

PaxDbiP12378.
PeptideAtlasiP12378.
PRIDEiP12378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000019302; ENSBTAP00000019302; ENSBTAG00000014521.
GeneIDi281564.
KEGGibta:281564.

Organism-specific databases

CTDi7358.

Phylogenomic databases

eggNOGiKOG2666. Eukaryota.
COG1004. LUCA.
GeneTreeiENSGT00390000015355.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiP12378.
KOiK00012.
OMAiVIIMNDY.
OrthoDBiEOG091G05RK.
TreeFamiTF105671.

Enzyme and pathway databases

UniPathwayiUPA00038; UER00491.
ReactomeiR-BTA-173599. Formation of the active cofactor, UDP-glucuronate.

Gene expression databases

BgeeiENSBTAG00000014521.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUGDH_BOVIN
AccessioniPrimary (citable) accession number: P12378
Secondary accession number(s): A6QR10, O77806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 15, 1998
Last modified: October 5, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.