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Reviewed, UniProtKB/Swiss-Prot P12378 (UGDH_BOVIN)

Last modified November 25, 2008. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    UDP-glucose 6-dehydrogenase
      Short name=UDP-Glc dehydrogenase
      Short name=UDP-GlcDH
      Short name=UDPGDH
    EC=1.1.1.22
Gene names
Name: UGDH
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Catalytic activity

UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH.

Pathway

Nucleotide-sugar biosynthesis; UDP-glucuronate biosynthesis; UDP-glucuronate from UDP-glucose: step 1/1.

Subunit structure

Homohexamer.

Sequence similarities

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494UDP-glucose 6-dehydrogenase
PRO_0000074059

Regions

Nucleotide binding6 – 2318NAD By similarity
Region162 – 1654Substrate binding By similarity

Sites

Active site2761Nucleophile By similarity
Binding site361NAD By similarity
Binding site411NAD By similarity
Binding site931NAD By similarity
Binding site1311NAD; via amide nitrogen By similarity
Binding site1651NAD By similarity
Binding site2201Substrate By similarity
Binding site2731Substrate; via amide nitrogen By similarity
Binding site2791NAD By similarity
Binding site3391Substrate By similarity
Binding site3461NAD By similarity

Amino acid modifications

Modified residue4731Phosphotyrosine By similarity

Experimental info

Sequence conflict1571Missing AA sequence Ref.3
Sequence conflict278 – 2814QKDV → ZZGK AA sequence Ref.4

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Sequences

Sequence LengthMass (Da)Tools
P12378-1 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 8560E27089C4D03E

FASTA49455,136
        10         20         30         40         50         60 
MFEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDINES RINAWNSPTL PIYEPGLKEV 

        70         80         90        100        110        120 
VESCRGKNLF FSTNIDDAIK EADLVFISVN TPTKTYGMGK GRAADLKYIE ACARRIVQNS 

       130        140        150        160        170        180 
HGYKIVTEKS TVPVRAAESI RRIFDANTKP NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI 

       190        200        210        220        230        240 
GGDETPEGQR AVQALCAVYE HWVPREKILT TNTWSSELSK LTANAFLAQR ISSINSISAL 

       250        260        270        280        290        300 
CEATGADVEE VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW 

       310        320        330        340        350        360 
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS IYISKYLMDE 

       370        380        390        400        410        420 
GAHLHIYDPK VPREQIVVDL SHPGVSKDDQ VARLVTISKD PYEACDGAHA VVICTEWDMF 

       430        440        450        460        470        480 
KELDYERIHK KMLKPAFIFD GRRVLDGLHN ELQTIGFQIE TIGKKVSSKR IPYAPSGEIP 

       490 
KFSLQDMPNK KPRV

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References

« Hide 'large scale' references
[1]"cDNA cloning and expression of UDP-glucose dehydrogenase from bovine kidney."
Lind T., Falk E., Hjertson E., Kusche-Gullberg M., Lidholt K.
Glycobiology 9:595-600(1999) [PubMed: 10336992] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.
[3]"UDP-glucose dehydrogenase from bovine liver: primary structure and relationship to other dehydrogenases."
Hempel J., Perozich J., Romovacek H., Hinich A., Kuo I., Feingold D.S.
Protein Sci. 3:1074-1080(1994) [PubMed: 7920253] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-469.
Tissue: Liver.
[4]"Amino acid sequence of the tryptic peptide containing the catalytic-site thiol group of bovine liver uridine diphosphate glucose dehydrogenase."
Franzen B., Carrubba C., Feingold D.S., Ashcom J., Franzen J.S.
Biochem. J. 199:599-602(1981) [PubMed: 6896145] [Abstract]
Cited for: PROTEIN SEQUENCE OF 268-281.
Tissue: Liver.

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Cross-references

Sequence databases

AF095792 mRNA. Translation: AAC64183.1.
BC150068 mRNA. Translation: AAI50069.1.
PIRA17150.
RefSeqNP_776636.1.
UniGeneBt.5538

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSBTAG00000014521. Bos taurus. [Contig view]
GeneID281564.
KEGGbta:281564.

Phylogenomic databases

HOVERGENP12378.

Family and domain databases

InterProIPR013328. DHase_multihelical.
IPR016040. NAD(P)-bd.
IPR017476. Nucleotide_sugar_DH.
IPR014027. UDP-Glc/GDP-Man_DHase_C.
IPR014026. UDP-Glc/GDP-Man_DHase_dimer.
IPR014028. UDP-Glc/GDP-Man_DHase_dimer-bd.
IPR001732. UDP-Glc/GDP-Man_DHase_N.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.
G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHERPTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
PfamPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameUGDH_BOVIN
AccessionPrimary (citable) accession number: P12378
Secondary accession number(s): A6QR10, O77806
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 15, 1998
Last modified: November 25, 2008
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents