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Protein

UDP-glucose 6-dehydrogenase

Gene

UGDH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

Enzyme regulationi

UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch.By similarity

Pathway:iUDP-alpha-D-glucuronate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose.
Proteins known to be involved in this subpathway in this organism are:
  1. UDP-glucose 6-dehydrogenase (UGDH)
This subpathway is part of the pathway UDP-alpha-D-glucuronate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose, the pathway UDP-alpha-D-glucuronate biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361NADBy similarity
Binding sitei41 – 411NADBy similarity
Binding sitei165 – 1651NADBy similarity
Active sitei276 – 2761NucleophileBy similarity
Binding sitei346 – 3461NADBy similarity
Binding sitei442 – 4421SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166NADBy similarity
Nucleotide bindingi89 – 935NADBy similarity
Nucleotide bindingi130 – 1312NADBy similarity
Nucleotide bindingi276 – 2794NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_305023. Formation of the active cofactor, UDP-glucuronate.
UniPathwayiUPA00038; UER00491.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
Short name:
UDP-Glc dehydrogenase
Short name:
UDP-GlcDH
Short name:
UDPGDH
Gene namesi
Name:UGDH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494UDP-glucose 6-dehydrogenasePRO_0000074059Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei107 – 1071N6-acetyllysineBy similarity
Modified residuei476 – 4761PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP12378.
PRIDEiP12378.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019302.

Structurei

3D structure databases

ProteinModelPortaliP12378.
SMRiP12378. Positions 1-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 1655Substrate bindingBy similarity
Regioni220 – 2278Substrate bindingBy similarity
Regioni260 – 27314Substrate bindingBy similarityAdd
BLAST
Regioni338 – 3392Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1004.
GeneTreeiENSGT00390000015355.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiP12378.
KOiK00012.
OMAiVIIMNDY.
OrthoDBiEOG7034GP.
TreeFamiTF105671.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PTHR11374:SF3. PTHR11374:SF3. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

P12378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDINES RINAWNSPTL
60 70 80 90 100
PIYEPGLKEV VESCRGKNLF FSTNIDDAIK EADLVFISVN TPTKTYGMGK
110 120 130 140 150
GRAADLKYIE ACARRIVQNS HGYKIVTEKS TVPVRAAESI RRIFDANTKP
160 170 180 190 200
NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI GGDETPEGQR AVQALCAVYE
210 220 230 240 250
HWVPREKILT TNTWSSELSK LTANAFLAQR ISSINSISAL CEATGADVEE
260 270 280 290 300
VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW
310 320 330 340 350
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS
360 370 380 390 400
IYISKYLMDE GAHLHIYDPK VPREQIVVDL SHPGVSKDDQ VARLVTISKD
410 420 430 440 450
PYEACDGAHA VVICTEWDMF KELDYERIHK KMLKPAFIFD GRRVLDGLHN
460 470 480 490
ELQTIGFQIE TIGKKVSSKR IPYAPSGEIP KFSLQDMPNK KPRV
Length:494
Mass (Da):55,136
Last modified:December 15, 1998 - v2
Checksum:i8560E27089C4D03E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti157 – 1571Missing AA sequence (PubMed:7920253).Curated
Sequence conflicti278 – 2814QKDV → ZZGK AA sequence (PubMed:6896145).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095792 mRNA. Translation: AAC64183.1.
BC150068 mRNA. Translation: AAI50069.1.
PIRiA17150.
RefSeqiNP_776636.1. NM_174211.3.
XP_005207890.1. XM_005207833.2.
UniGeneiBt.5538.

Genome annotation databases

EnsembliENSBTAT00000019302; ENSBTAP00000019302; ENSBTAG00000014521.
GeneIDi281564.
KEGGibta:281564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095792 mRNA. Translation: AAC64183.1.
BC150068 mRNA. Translation: AAI50069.1.
PIRiA17150.
RefSeqiNP_776636.1. NM_174211.3.
XP_005207890.1. XM_005207833.2.
UniGeneiBt.5538.

3D structure databases

ProteinModelPortaliP12378.
SMRiP12378. Positions 1-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019302.

Proteomic databases

PaxDbiP12378.
PRIDEiP12378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000019302; ENSBTAP00000019302; ENSBTAG00000014521.
GeneIDi281564.
KEGGibta:281564.

Organism-specific databases

CTDi7358.

Phylogenomic databases

eggNOGiCOG1004.
GeneTreeiENSGT00390000015355.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiP12378.
KOiK00012.
OMAiVIIMNDY.
OrthoDBiEOG7034GP.
TreeFamiTF105671.

Enzyme and pathway databases

UniPathwayiUPA00038; UER00491.
ReactomeiREACT_305023. Formation of the active cofactor, UDP-glucuronate.

Miscellaneous databases

NextBioi20805514.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PTHR11374:SF3. PTHR11374:SF3. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression of UDP-glucose dehydrogenase from bovine kidney."
    Lind T., Falk E., Hjertson E., Kusche-Gullberg M., Lidholt K.
    Glycobiology 9:595-600(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.
  3. "UDP-glucose dehydrogenase from bovine liver: primary structure and relationship to other dehydrogenases."
    Hempel J., Perozich J., Romovacek H., Hinich A., Kuo I., Feingold D.S.
    Protein Sci. 3:1074-1080(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-469, SUBUNIT.
    Tissue: Liver.
  4. "Amino acid sequence of the tryptic peptide containing the catalytic-site thiol group of bovine liver uridine diphosphate glucose dehydrogenase."
    Franzen B., Carrubba C., Feingold D.S., Ashcom J., Franzen J.S.
    Biochem. J. 199:599-602(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 268-281.
    Tissue: Liver.

Entry informationi

Entry nameiUGDH_BOVIN
AccessioniPrimary (citable) accession number: P12378
Secondary accession number(s): A6QR10, O77806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 15, 1998
Last modified: June 24, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.