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P12378 (UGDH_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glucose 6-dehydrogenase

Short name=UDP-Glc dehydrogenase
Short name=UDP-GlcDH
Short name=UDPGDH
EC=1.1.1.22
Gene names
Name:UGDH
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.

Catalytic activity

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

Enzyme regulation

UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch By similarity.

Pathway

Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.

Subunit structure

Homohexamer. Ref.3

Sequence similarities

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494UDP-glucose 6-dehydrogenase
PRO_0000074059

Regions

Nucleotide binding11 – 166NAD By similarity
Nucleotide binding89 – 935NAD By similarity
Nucleotide binding130 – 1312NAD By similarity
Nucleotide binding276 – 2794NAD By similarity
Region161 – 1655Substrate binding By similarity
Region220 – 2278Substrate binding By similarity
Region260 – 27314Substrate binding By similarity
Region338 – 3392Substrate binding By similarity

Sites

Active site2761Nucleophile By similarity
Binding site361NAD By similarity
Binding site411NAD By similarity
Binding site1651NAD By similarity
Binding site3461NAD By similarity
Binding site4421Substrate By similarity

Amino acid modifications

Modified residue1071N6-acetyllysine By similarity

Experimental info

Sequence conflict1571Missing AA sequence Ref.3
Sequence conflict278 – 2814QKDV → ZZGK AA sequence Ref.4

Sequences

Sequence LengthMass (Da)Tools
P12378 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 8560E27089C4D03E

FASTA49455,136
        10         20         30         40         50         60 
MFEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDINES RINAWNSPTL PIYEPGLKEV 

        70         80         90        100        110        120 
VESCRGKNLF FSTNIDDAIK EADLVFISVN TPTKTYGMGK GRAADLKYIE ACARRIVQNS 

       130        140        150        160        170        180 
HGYKIVTEKS TVPVRAAESI RRIFDANTKP NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI 

       190        200        210        220        230        240 
GGDETPEGQR AVQALCAVYE HWVPREKILT TNTWSSELSK LTANAFLAQR ISSINSISAL 

       250        260        270        280        290        300 
CEATGADVEE VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW 

       310        320        330        340        350        360 
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS IYISKYLMDE 

       370        380        390        400        410        420 
GAHLHIYDPK VPREQIVVDL SHPGVSKDDQ VARLVTISKD PYEACDGAHA VVICTEWDMF 

       430        440        450        460        470        480 
KELDYERIHK KMLKPAFIFD GRRVLDGLHN ELQTIGFQIE TIGKKVSSKR IPYAPSGEIP 

       490 
KFSLQDMPNK KPRV 

« Hide

References

« Hide 'large scale' references
[1]"cDNA cloning and expression of UDP-glucose dehydrogenase from bovine kidney."
Lind T., Falk E., Hjertson E., Kusche-Gullberg M., Lidholt K.
Glycobiology 9:595-600(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Ascending colon.
[3]"UDP-glucose dehydrogenase from bovine liver: primary structure and relationship to other dehydrogenases."
Hempel J., Perozich J., Romovacek H., Hinich A., Kuo I., Feingold D.S.
Protein Sci. 3:1074-1080(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-469, SUBUNIT.
Tissue: Liver.
[4]"Amino acid sequence of the tryptic peptide containing the catalytic-site thiol group of bovine liver uridine diphosphate glucose dehydrogenase."
Franzen B., Carrubba C., Feingold D.S., Ashcom J., Franzen J.S.
Biochem. J. 199:599-602(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 268-281.
Tissue: Liver.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF095792 mRNA. Translation: AAC64183.1.
BC150068 mRNA. Translation: AAI50069.1.
PIRA17150.
RefSeqNP_776636.1. NM_174211.3.
XP_005207890.1. XM_005207833.1.
UniGeneBt.5538.

3D structure databases

ProteinModelPortalP12378.
SMRP12378. Positions 1-466.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP12378.
PRIDEP12378.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000019302; ENSBTAP00000019302; ENSBTAG00000014521.
GeneID281564.
KEGGbta:281564.

Organism-specific databases

CTD7358.

Phylogenomic databases

eggNOGCOG1004.
GeneTreeENSGT00390000015355.
HOGENOMHOG000153773.
HOVERGENHBG003512.
InParanoidP12378.
KOK00012.
OMAAIGRSTQ.
OrthoDBEOG7034GP.
TreeFamTF105671.

Enzyme and pathway databases

UniPathwayUPA00038; UER00491.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERPTHR11374. PTHR11374. 1 hit.
PTHR11374:SF3. PTHR11374:SF3. 1 hit.
PfamPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetSearch...

Other

NextBio20805514.

Entry information

Entry nameUGDH_BOVIN
AccessionPrimary (citable) accession number: P12378
Secondary accession number(s): A6QR10, O77806
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 15, 1998
Last modified: July 9, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways