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Protein

cAMP-dependent protein kinase catalytic subunit

Gene

Pka-C1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by cAMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751ATPPROSITE-ProRule annotation
Active sitei169 – 1691Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi52 – 609ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase activity Source: FlyBase
  • protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  • anesthesia-resistant memory Source: FlyBase
  • anterior/posterior pattern specification, imaginal disc Source: FlyBase
  • behavioral response to ethanol Source: FlyBase
  • cAMP-mediated signaling Source: FlyBase
  • compound eye development Source: FlyBase
  • imaginal disc-derived leg morphogenesis Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • learning Source: FlyBase
  • learning or memory Source: FlyBase
  • locomotor rhythm Source: FlyBase
  • memory Source: FlyBase
  • modulation of synaptic transmission Source: FlyBase
  • negative regulation of smoothened signaling pathway Source: FlyBase
  • olfactory learning Source: FlyBase
  • oocyte anterior/posterior axis specification Source: FlyBase
  • oocyte microtubule cytoskeleton polarization Source: FlyBase
  • oogenesis Source: FlyBase
  • positive regulation of hh target transcription factor activity Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of apoptotic process Source: FlyBase
  • regulation of bicoid mRNA localization Source: FlyBase
  • regulation of circadian sleep/wake cycle, sleep Source: FlyBase
  • regulation of pole plasm oskar mRNA localization Source: FlyBase
  • rhythmic behavior Source: FlyBase
  • thermosensory behavior Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, cAMP, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.11. 1994.
ReactomeiR-DME-163615. PKA activation.
R-DME-164378. PKA activation in glucagon signalling.
R-DME-180024. DARPP-32 events.
R-DME-194223. HDL-mediated lipid transport.
R-DME-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-DME-392517. Rap1 signalling.
R-DME-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5610787. Hedgehog 'off' state.
R-DME-5621575. CD209 (DC-SIGN) signaling.
R-DME-983231. Factors involved in megakaryocyte development and platelet production.
SignaLinkiP12370.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase catalytic subunit (EC:2.7.11.11)
Short name:
PKA C
Gene namesi
Name:Pka-C1
Synonyms:CdkA, DC0
ORF Names:CG4379
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000273. Pka-C1.

Subcellular locationi

GO - Cellular componenti

  • cAMP-dependent protein kinase complex Source: FlyBase
  • cell body Source: FlyBase
  • cytoplasm Source: FlyBase
  • dendrite Source: FlyBase
  • plasma membrane Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 353352cAMP-dependent protein kinase catalytic subunitPRO_0000086069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP12370.
PRIDEiP12370.

Expressioni

Tissue specificityi

More abundant in adult head than adult body.

Gene expression databases

BgeeiFBgn0000273.
GenevisibleiP12370. DM.

Interactioni

Subunit structurei

Composed of two regulatory chains and two catalytic chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
CalpBQ9VT652EBI-82224,EBI-132069
sloQ037205EBI-82224,EBI-426805

Protein-protein interaction databases

BioGridi60383. 13 interactions.
DIPiDIP-23727N.
IntActiP12370. 7 interactions.
MINTiMINT-1019424.
STRINGi7227.FBpp0079448.

Structurei

3D structure databases

ProteinModelPortaliP12370.
SMRiP12370. Positions 18-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 300255Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini301 – 35353AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0616. Eukaryota.
ENOG410XPQQ. LUCA.
GeneTreeiENSGT00810000125385.
InParanoidiP12370.
KOiK04345.
OMAiNAATANK.
OrthoDBiEOG091G10O8.
PhylomeDBiP12370.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNNATTSNK KVDAAETVKE FLEQAKEEFE DKWRRNPTNT AALDDFERIK
60 70 80 90 100
TLGTGSFGRV MIVQHKPTKD YYAMKILDKQ KVVKLKQVEH TLNEKRILQA
110 120 130 140 150
IQFPFLVSLR YHFKDNSNLY MVLEYVPGGE MFSHLRKVGR FSEPHSRFYA
160 170 180 190 200
AQIVLAFEYL HYLDLIYRDL KPENLLIDSQ GYLKVTDFGF AKRVKGRTWT
210 220 230 240 250
LCGTPEYLAP EIILSKGYNK AVDWWALGVL VYEMAAGYPP FFADQPIQIY
260 270 280 290 300
EKIVSGKVRF PSHFGSDLKD LLRNLLQVDL TKRYGNLKAG VNDIKNQKWF
310 320 330 340 350
ASTDWIAIFQ KKIEAPFIPR CKGPGDTSNF DDYEEEALRI SSTEKCAKEF

AEF
Length:353
Mass (Da):40,839
Last modified:January 23, 2007 - v3
Checksum:i9611D1001C791874
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18655 Genomic DNA. Translation: AAA28412.1.
X16969 Genomic DNA. Translation: CAA34840.1.
AE014134 Genomic DNA. Translation: AAF52797.1.
AE014134 Genomic DNA. Translation: AAN10703.1.
AE014134 Genomic DNA. Translation: AAS64669.1.
AY069425 mRNA. Translation: AAL39570.1.
PIRiC31751.
RefSeqiNP_476977.1. NM_057629.4.
NP_723479.1. NM_164866.3.
NP_995672.1. NM_205950.3.
UniGeneiDm.2136.

Genome annotation databases

EnsemblMetazoaiFBtr0079851; FBpp0079448; FBgn0000273.
FBtr0079852; FBpp0089382; FBgn0000273.
FBtr0335495; FBpp0307466; FBgn0000273.
GeneIDi34284.
KEGGidme:Dmel_CG4379.
UCSCiCG4379-RB. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18655 Genomic DNA. Translation: AAA28412.1.
X16969 Genomic DNA. Translation: CAA34840.1.
AE014134 Genomic DNA. Translation: AAF52797.1.
AE014134 Genomic DNA. Translation: AAN10703.1.
AE014134 Genomic DNA. Translation: AAS64669.1.
AY069425 mRNA. Translation: AAL39570.1.
PIRiC31751.
RefSeqiNP_476977.1. NM_057629.4.
NP_723479.1. NM_164866.3.
NP_995672.1. NM_205950.3.
UniGeneiDm.2136.

3D structure databases

ProteinModelPortaliP12370.
SMRiP12370. Positions 18-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60383. 13 interactions.
DIPiDIP-23727N.
IntActiP12370. 7 interactions.
MINTiMINT-1019424.
STRINGi7227.FBpp0079448.

Proteomic databases

PaxDbiP12370.
PRIDEiP12370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079851; FBpp0079448; FBgn0000273.
FBtr0079852; FBpp0089382; FBgn0000273.
FBtr0335495; FBpp0307466; FBgn0000273.
GeneIDi34284.
KEGGidme:Dmel_CG4379.
UCSCiCG4379-RB. d. melanogaster.

Organism-specific databases

CTDi34284.
FlyBaseiFBgn0000273. Pka-C1.

Phylogenomic databases

eggNOGiKOG0616. Eukaryota.
ENOG410XPQQ. LUCA.
GeneTreeiENSGT00810000125385.
InParanoidiP12370.
KOiK04345.
OMAiNAATANK.
OrthoDBiEOG091G10O8.
PhylomeDBiP12370.

Enzyme and pathway databases

BRENDAi2.7.11.11. 1994.
ReactomeiR-DME-163615. PKA activation.
R-DME-164378. PKA activation in glucagon signalling.
R-DME-180024. DARPP-32 events.
R-DME-194223. HDL-mediated lipid transport.
R-DME-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-DME-392517. Rap1 signalling.
R-DME-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5610787. Hedgehog 'off' state.
R-DME-5621575. CD209 (DC-SIGN) signaling.
R-DME-983231. Factors involved in megakaryocyte development and platelet production.
SignaLinkiP12370.

Miscellaneous databases

GenomeRNAii34284.
PROiP12370.

Gene expression databases

BgeeiFBgn0000273.
GenevisibleiP12370. DM.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAPC_DROME
AccessioniPrimary (citable) accession number: P12370
Secondary accession number(s): A4V0I0, Q9VL99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 168 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.