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P12369

- KAP3_RAT

UniProt

P12369 - KAP3_RAT

Protein

cAMP-dependent protein kinase type II-beta regulatory subunit

Gene

Prkar2b

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei221 – 2211cAMP 1
    Binding sitei230 – 2301cAMP 1
    Binding sitei350 – 3501cAMP 2
    Binding sitei359 – 3591cAMP 2

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi152 – 273122cAMP 1Add
    BLAST
    Nucleotide bindingi274 – 416143cAMP 2Add
    BLAST

    GO - Molecular functioni

    1. cAMP binding Source: RGD
    2. cAMP-dependent protein kinase regulator activity Source: RGD
    3. protein binding Source: IntAct
    4. protein domain specific binding Source: RGD
    5. protein kinase A catalytic subunit binding Source: RGD

    GO - Biological processi

    1. regulation of protein kinase activity Source: RGD
    2. response to antipsychotic drug Source: RGD
    3. response to clozapine Source: RGD

    Keywords - Ligandi

    cAMP, cAMP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196232. Factors involved in megakaryocyte development and platelet production.
    REACT_198294. Regulation of PLK1 Activity at G2/M Transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-dependent protein kinase type II-beta regulatory subunit
    Gene namesi
    Name:Prkar2b
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3394. Prkar2b.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity
    Note: Colocalizes with PJA2 in the cytoplasm and at the cell membrane.By similarity

    GO - Cellular componenti

    1. cAMP-dependent protein kinase complex Source: RGD
    2. cytoplasm Source: RGD
    3. membrane raft Source: RGD
    4. mitochondrial inner membrane Source: RGD
    5. perinuclear region of cytoplasm Source: RGD
    6. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedCurated
    Chaini2 – 416415cAMP-dependent protein kinase type II-beta regulatory subunitPRO_0000205392Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineCurated
    Modified residuei83 – 831PhosphoserineBy similarity
    Modified residuei85 – 851PhosphoserineBy similarity
    Modified residuei112 – 1121Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by the activated catalytic chain.1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP12369.
    PRIDEiP12369.

    PTM databases

    PhosphoSiteiP12369.

    Expressioni

    Tissue specificityi

    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Brain. Present in a few pyramidal neurons and mostly in mossy fibers. Colocalizes with PJA2 in dentate granule cells and at postsynaptic sites of primary hippocampal neurons.1 Publication

    Gene expression databases

    GenevestigatoriP12369.

    Interactioni

    Subunit structurei

    The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with the phosphorylated form of PJA2 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LRRK2Q5S0073EBI-6096160,EBI-5323863From a different organism.
    PRKACAP176122EBI-6096160,EBI-476586From a different organism.

    Protein-protein interaction databases

    BioGridi246811. 1 interaction.
    IntActiP12369. 2 interactions.
    STRINGi10116.ENSRNOP00000012415.

    Structurei

    Secondary structure

    1
    416
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi137 – 14711
    Helixi151 – 1544
    Helixi158 – 16710
    Beta strandi169 – 1735
    Beta strandi178 – 1803
    Beta strandi188 – 20316
    Beta strandi206 – 21510
    Helixi221 – 2244
    Beta strandi231 – 24616
    Helixi247 – 25913
    Helixi273 – 2753
    Beta strandi276 – 2783
    Helixi280 – 28910
    Beta strandi291 – 2955
    Beta strandi300 – 3023
    Beta strandi310 – 32415
    Beta strandi336 – 3427
    Beta strandi347 – 3493
    Helixi351 – 3555
    Beta strandi360 – 37516
    Helixi376 – 3827
    Helixi384 – 3863
    Helixi387 – 40519

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CX4X-ray2.45A112-416[»]
    3IDBX-ray1.62B108-268[»]
    3IDCX-ray2.70B102-265[»]
    4JVAX-ray2.50A112-416[»]
    ProteinModelPortaliP12369.
    SMRiP12369. Positions 3-41, 130-412.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP12369.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 151150Dimerization and phosphorylationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0664.
    HOGENOMiHOG000196668.
    HOVERGENiHBG002025.
    InParanoidiP12369.
    KOiK04739.
    OrthoDBiEOG76T9RR.
    PhylomeDBiP12369.
    TreeFamiTF314920.

    Family and domain databases

    Gene3Di2.60.120.10. 2 hits.
    InterProiIPR002373. cAMP/cGMP_kin.
    IPR012198. cAMP_dep_PK_reg_su.
    IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
    IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 2 hits.
    PF02197. RIIa. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000548. PK_regulatory. 1 hit.
    PRINTSiPR00103. CAMPKINASE.
    SMARTiSM00100. cNMP. 2 hits.
    SM00394. RIIa. 1 hit.
    [Graphical view]
    SUPFAMiSSF47391. SSF47391. 1 hit.
    SSF51206. SSF51206. 2 hits.
    PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
    PS00889. CNMP_BINDING_2. 2 hits.
    PS50042. CNMP_BINDING_3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12369-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIEIPAGLT ELLQGFTVEV LRHQPADLLE FALQHFTRLQ QENERKGAAR    50
    FGHEGRTWGD AGAAAGGGTP SKGVNFAEEP MRSDSENGEE EEAAEAGAFN 100
    APVINRFTRR ASVCAEAYNP DEEEDDAESR IIHPKTDDQR NRLQEACKDI 150
    LLFKNLDPEQ MSQVLDAMFE KLVKEGEHVI DQGDDGDNFY VIDRGTFDIY 200
    VKCDGVGRCV GNYDNRGSFG ELALMYNTPR AATITATSPG ALWGLDRVTF 250
    RRIIVKNNAK KRKMYESFIE SLPFLKSLEV SERLKVVDVI GTKVYNDGEQ 300
    IIAQGDSADS FFIVESGEVR ITMKRKGKSD IEENGAVEIA RCLRGQYFGE 350
    LALVTNKPRA ASAHAIGTVK CLAMDVQAFE RLLGPCMEIM KRNIATYEEQ 400
    LVALFGTNMD IVEPTA 416
    Length:416
    Mass (Da):46,123
    Last modified:January 23, 2007 - v3
    Checksum:i8BC984E325FA3612
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12492 mRNA. Translation: AAA42047.1.
    PIRiA28893. OKRTR2.
    RefSeqiNP_001025191.1. NM_001030020.1.
    UniGeneiRn.164375.
    Rn.4075.

    Genome annotation databases

    GeneIDi24679.
    KEGGirno:24679.
    UCSCiRGD:3394. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M12492 mRNA. Translation: AAA42047.1 .
    PIRi A28893. OKRTR2.
    RefSeqi NP_001025191.1. NM_001030020.1.
    UniGenei Rn.164375.
    Rn.4075.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CX4 X-ray 2.45 A 112-416 [» ]
    3IDB X-ray 1.62 B 108-268 [» ]
    3IDC X-ray 2.70 B 102-265 [» ]
    4JVA X-ray 2.50 A 112-416 [» ]
    ProteinModelPortali P12369.
    SMRi P12369. Positions 3-41, 130-412.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246811. 1 interaction.
    IntActi P12369. 2 interactions.
    STRINGi 10116.ENSRNOP00000012415.

    PTM databases

    PhosphoSitei P12369.

    Proteomic databases

    PaxDbi P12369.
    PRIDEi P12369.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24679.
    KEGGi rno:24679.
    UCSCi RGD:3394. rat.

    Organism-specific databases

    CTDi 5577.
    RGDi 3394. Prkar2b.

    Phylogenomic databases

    eggNOGi COG0664.
    HOGENOMi HOG000196668.
    HOVERGENi HBG002025.
    InParanoidi P12369.
    KOi K04739.
    OrthoDBi EOG76T9RR.
    PhylomeDBi P12369.
    TreeFami TF314920.

    Enzyme and pathway databases

    Reactomei REACT_196232. Factors involved in megakaryocyte development and platelet production.
    REACT_198294. Regulation of PLK1 Activity at G2/M Transition.

    Miscellaneous databases

    EvolutionaryTracei P12369.
    NextBioi 604075.
    PROi P12369.

    Gene expression databases

    Genevestigatori P12369.

    Family and domain databases

    Gene3Di 2.60.120.10. 2 hits.
    InterProi IPR002373. cAMP/cGMP_kin.
    IPR012198. cAMP_dep_PK_reg_su.
    IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
    IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 2 hits.
    PF02197. RIIa. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000548. PK_regulatory. 1 hit.
    PRINTSi PR00103. CAMPKINASE.
    SMARTi SM00100. cNMP. 2 hits.
    SM00394. RIIa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47391. SSF47391. 1 hit.
    SSF51206. SSF51206. 2 hits.
    PROSITEi PS00888. CNMP_BINDING_1. 2 hits.
    PS00889. CNMP_BINDING_2. 2 hits.
    PS50042. CNMP_BINDING_3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, cDNA structure and deduced amino acid sequence for the hormone-induced regulatory subunit (RII beta) of cAMP-dependent protein kinase from rat ovarian granulosa cells."
      Sandberg M., Levy F.O., Oeyen O., Hansson V., Jahnsen T.
      Biochem. Biophys. Res. Commun. 154:705-711(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning, cDNA structure, and regulation of the regulatory subunit of type II cAMP-dependent protein kinase from rat ovarian granulosa cells."
      Jahnsen T., Hedin L., Kidd V.J., Beattie W.G., Lohmann S.M., Walter U., Durica J., Schulz T.Z., Schiltz E., Browner M., Lawrence C.B., Goldman D., Ratoosh S.L., Richards J.S.
      J. Biol. Chem. 261:12352-12361(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE OF 16-416.
      Tissue: Ovarian granulosa cell.
    3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
      Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
      Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    4. Cited for: TISSUE SPECIFICITY.
    5. "Molecular basis for regulatory subunit diversity in cAMP-dependent protein kinase: crystal structure of the type II beta regulatory subunit."
      Diller T.C., Madhusudan X., Xuong N.H., Taylor S.S.
      Structure 9:73-82(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.45 ANGSTROMS) OF 112-416.

    Entry informationi

    Entry nameiKAP3_RAT
    AccessioniPrimary (citable) accession number: P12369
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3