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P12368

- KAP2_RAT

UniProt

P12368 - KAP2_RAT

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Protein

cAMP-dependent protein kinase type II-alpha regulatory subunit

Gene

Prkar2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei205 – 2051cAMP 1By similarity
Binding sitei214 – 2141cAMP 1By similarity
Binding sitei335 – 3351cAMP 2By similarity
Binding sitei344 – 3441cAMP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi136 – 257122cAMP 1By similarityAdd
BLAST
Nucleotide bindingi258 – 401144cAMP 2By similarityAdd
BLAST

GO - Molecular functioni

  1. cAMP binding Source: RGD
  2. cAMP-dependent protein kinase regulator activity Source: RGD
  3. protein domain specific binding Source: RGD
  4. protein homodimerization activity Source: RGD
  5. protein kinase A catalytic subunit binding Source: RGD
  6. small molecule binding Source: RGD

GO - Biological processi

  1. protein heterooligomerization Source: RGD
  2. regulation of protein kinase activity Source: RGD
  3. response to clozapine Source: RGD
Complete GO annotation...

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_231505. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type II-alpha regulatory subunit
Gene namesi
Name:Prkar2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3393. Prkar2a.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity
Note: Colocalizes with PJA2 in the cytoplasm and the cell membrane.By similarity

GO - Cellular componenti

  1. cAMP-dependent protein kinase complex Source: RGD
  2. cytoplasm Source: RGD
  3. perinuclear region of cytoplasm Source: RGD
  4. protein complex Source: RGD
  5. T-tubule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 401400cAMP-dependent protein kinase type II-alpha regulatory subunitPRO_0000205388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei48 – 481PhosphoserineBy similarity
Modified residuei75 – 751PhosphoserineBy similarity
Modified residuei77 – 771PhosphoserineBy similarity
Modified residuei97 – 971Phosphoserine1 Publication
Modified residuei212 – 2121Phosphothreonine; by PDPK1By similarity

Post-translational modificationi

Phosphorylated by the activated catalytic chain.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP12368.
PRIDEiP12368.

PTM databases

PhosphoSiteiP12368.

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

GenevestigatoriP12368.

Interactioni

Subunit structurei

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3 (By similarity). Interacts with the phosphorylated form of PJA2 (By similarity). Interacts with MYRIP; his interaction may link PKA to components of the exocytosis machinery, thus facilitating exocytosis, including insulin release.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-555N.
IntActiP12368. 4 interactions.
MINTiMINT-4564663.
STRINGi10116.ENSRNOP00000027552.

Structurei

Secondary structure

1
401
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 2415Combined sources
Helixi29 – 4315Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DRNNMR-A/B1-45[»]
2H9RNMR-A/B1-45[»]
2HWNX-ray1.60A/B/C/D1-45[»]
3TMHX-ray3.80B/C/F/G1-45[»]
ProteinModelPortaliP12368.
SMRiP12368. Positions 1-45, 93-393.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12368.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 135134Dimerization and phosphorylationAdd
BLAST

Sequence similaritiesi

Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000196668.
HOVERGENiHBG002025.
InParanoidiP12368.
KOiK04739.
PhylomeDBiP12368.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR002373. cAMP/cGMP_kin.
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
PRINTSiPR00103. CAMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12368-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHIQIPPGL TELLQGYTVE VLRQQPPDLV DFAVEYFTRL REARRQESDS
60 70 80 90 100
FIAPPTTFHA QESSGVPVIE EDGESESDSD DEDLEVPIPS KFTRRVSVCA
110 120 130 140 150
ETFNPDEEED NDPRVVHPKT DEQRYRLQEA CKDILLFKNL DQEQLSQVLD
160 170 180 190 200
AMFEKIVKTD EHVIDQGDDG DNFYVIERGT YDILVTKDNQ TRSVGQYDNR
210 220 230 240 250
GSFGELALMY NTPRAATIVA TSDGSLWGLD RVTFRRIIVK NNAKKRKMFE
260 270 280 290 300
SFIESVPLFK SLEMSERMKI VDVIGEKIYK DGERIITQGE KADSFYIIES
310 320 330 340 350
GEVSILIRSK TKTNKNGGNQ EVEIAHCHKG QYFGELALVT NKPRAASAYA
360 370 380 390 400
VGDVKCLVMD VQAFERLLGP CMDIMKRNIS HYEEQLVKMF GSNLDLLDPG

Q
Length:401
Mass (Da):45,540
Last modified:January 23, 2007 - v3
Checksum:i026FEBFCFACA9C7D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741E → Q in AAA41856. (PubMed:3037538)Curated
Sequence conflicti90 – 901S → A in AAA41856. (PubMed:3037538)Curated
Sequence conflicti125 – 1251Y → C in AAA41856. (PubMed:3037538)Curated
Sequence conflicti154 – 1552EK → KR in AAA41856. (PubMed:3037538)Curated
Sequence conflicti198 – 1981D → A in AAA41856. (PubMed:3037538)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533978 mRNA. Translation: AAM97689.1.
J02934 mRNA. Translation: AAA41856.1.
PIRiA28325. OKRT2R.
RefSeqiNP_062137.1. NM_019264.2.
UniGeneiRn.9742.

Genome annotation databases

GeneIDi29699.
KEGGirno:29699.
UCSCiRGD:3393. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF533978 mRNA. Translation: AAM97689.1 .
J02934 mRNA. Translation: AAA41856.1 .
PIRi A28325. OKRT2R.
RefSeqi NP_062137.1. NM_019264.2.
UniGenei Rn.9742.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DRN NMR - A/B 1-45 [» ]
2H9R NMR - A/B 1-45 [» ]
2HWN X-ray 1.60 A/B/C/D 1-45 [» ]
3TMH X-ray 3.80 B/C/F/G 1-45 [» ]
ProteinModelPortali P12368.
SMRi P12368. Positions 1-45, 93-393.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-555N.
IntActi P12368. 4 interactions.
MINTi MINT-4564663.
STRINGi 10116.ENSRNOP00000027552.

PTM databases

PhosphoSitei P12368.

Proteomic databases

PaxDbi P12368.
PRIDEi P12368.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29699.
KEGGi rno:29699.
UCSCi RGD:3393. rat.

Organism-specific databases

CTDi 5576.
RGDi 3393. Prkar2a.

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000196668.
HOVERGENi HBG002025.
InParanoidi P12368.
KOi K04739.
PhylomeDBi P12368.

Enzyme and pathway databases

Reactomei REACT_231505. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.

Miscellaneous databases

EvolutionaryTracei P12368.
NextBioi 610099.
PROi P12368.

Gene expression databases

Genevestigatori P12368.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
InterProi IPR002373. cAMP/cGMP_kin.
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view ]
PIRSFi PIRSF000548. PK_regulatory. 1 hit.
PRINTSi PR00103. CAMPKINASE.
SMARTi SM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view ]
SUPFAMi SSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEi PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence and complete coding region of cAMP-dependent protein kinase type II regulatory subunit from a rat testicular germ cell cDNA library."
    Ziemba A.J., Collard M.W.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain."
    Scott J.D., Glaccum M.B., Zoller M.J., Uhler M.D., Helfman D.M., McKnight G.S., Krebs E.G.
    Proc. Natl. Acad. Sci. U.S.A. 84:5192-5196(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 32-401.
    Tissue: Skeletal muscle.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. Cited for: INTERACTION WITH MYRIP.

Entry informationi

Entry nameiKAP2_RAT
AccessioniPrimary (citable) accession number: P12368
Secondary accession number(s): Q8K1M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3