Reviewed, UniProtKB/Swiss-Prot P12365 (CATA2_MAIZE)

Names and origin

Protein names

Recommended name:
    Catalase isozyme 2
    EC=1.11.1.6

Gene names

Name:

CAT2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.
Catalytic activity	2 H2O2 = O2 + 2 H2O.
Cofactor	Heme group.
Subunit structure	Homotetramer.
Subcellular location	Peroxisome. Cytoplasm.
Sequence similarities	Belongs to the catalase family.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Cytoplasm Peroxisome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Catalase isozyme 2		PRO_0000084947
Sites
	Active site	64	1	By similarity
	Active site	137	1	By similarity
	Metal binding	347	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	296	1	T → R
Experimental info
	Sequence conflict	14	1	A → P in AAA33440. Ref.3
	Sequence conflict	124	1	K → N in AAA33440. Ref.3
	Sequence conflict	241 – 242	2	RC → AVG Ref.2
	Sequence conflict	296	1	T → A Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12365-1 [UniParc]. Last modified April 1, 1993. Version 3. Checksum: 6B686B6A94EA8598	FASTA	491	56,466
	10 20 30 40 50 60 MDPYKHRPSS AFNAPYWTTN SGAPVWNNDS SLTVGARGPI LLEDYHCEKL ANFDRERIPE 70 80 90 100 110 120 RVVHARGASA KGFFEVTHDI THLTCADFLR APGVQTPVIV RFSTVIHERG SPETLRDPRG 130 140 150 160 170 180 FAVKFYTREG NWDLVGNNFP VFFIRDGIKF PDMVHALKPN PRTHIQDNWR ILDFFSHHPE 190 200 210 220 230 240 SLHMFSFLFD DVGIPADYRH MDGSGVHTYT LVSRAGTVTY VKFHWRPTCG VRSLMDDEAV 250 260 270 280 290 300 RCGANHSHAT KDLTDAIAAG NFPEWTLYIQ TMDPEMEDRL DDLDPLDVTK TWPEDTFPLQ 310 320 330 340 350 360 PVGRLVLNRN IDNFFAENEQ LAFCPGLIVP GIYYSDDKLL QTRIFSYSDT QRHRLGPNYL 370 380 390 400 410 420 LLPANAPKCA HHNNHYDGSM NFMHRHEEVD YFPSRYDAVR NAPRYPIPTA HIAGRREKTV 430 440 450 460 470 480 ISKENNFKQP GERYRAMDPA RQERFITRWV DALSDPRLTH EIRTIWLSNW SQADRSLGQK 490 LASRLSAKPS M

References

[1]	"Comparison of the cat2 complementary DNA sequences of a normal catalase activity line (W64A) and a high catalase activity line (R6-67) of maize." Guan L., Ruzsa S., Skadsen R.W., Scandalios J.G. Plant Physiol. 96:1379-1381(1991) [PubMed: 16668346] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Wisconsin 64A.
[2]	"Isolation, characterization and expression of the maize Cat2 catalase gene." Guan L., Polidoros A.N., Scandalios J.G. Plant Mol. Biol. 30:913-924(1996) [PubMed: 8639750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wisconsin 64A. Tissue: Leaf.
[3]	"Isolation and characterization of a cDNA clone for the Cat2 gene in maize and its homology with other catalases." Bethards L.A., Skadsen R.W., Scandalios J.G. Proc. Natl. Acad. Sci. U.S.A. 84:6830-6834(1987) [PubMed: 2821546] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. R6-67.
[4]	"Isolation and characterization of a cDNA clone for the Cat2 gene in maize and its homology with other catalases." Bethards L.A., Skadsen R.W., Scandalios J.G. Proc. Natl. Acad. Sci. U.S.A. 87:6927-6927(1990) [PubMed: 2395887] [Abstract] Cited for: SEQUENCE REVISION TO C-TERMINUS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54819 mRNA. Translation: CAA38588.1. Z54358 Genomic DNA. No translation available. J02976 mRNA. Translation: AAA33440.1.
PIR	S71455.
RefSeq	NP_001105310.1.
UniGene	Zm.157
3D structure databases
HSSP	HSSP built from PDB template 1M7S based on UniProtKB P46206.
ModBase	Search...
Protein family/group databases
PeroxiBase	6438. ZmKat2.
Genome annotation databases
GeneID	542230.
Organism-specific databases
Gramene	P12365.
MaizeGDB	13855.
Enzyme and pathway databases
BRENDA	1.11.1.6. 289.
Family and domain databases
InterPro	IPR002226. Catalase. IPR010582. Catalase-rel_immune_responsive. IPR011614. Catalase_N. IPR018028. Catalase_rel_subgroup. [Graphical view]
Gene3D	G3DSA:2.40.180.10. Catalase_N. 1 hit.
PANTHER	PTHR11465. Catalase. 1 hit.
Pfam	PF00199. Catalase. 1 hit. PF06628. Catalase-rel. 1 hit. [Graphical view]
PRINTS	PR00067. CATALASE.
ProDom	PD000510. Catalase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00437. CATALASE_1. 1 hit. PS00438. CATALASE_2. 1 hit. PS51402. CATALASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATA2_MAIZE

Accession

Primary (citable) accession number: P12365

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)