P12364 (T3MO_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type III restriction-modification system EcoP15I enzyme mod
Short name=M.EcoP15I
EC=2.1.1.72

Alternative name(s):
EcoP15I methyltransferase

Gene names

Name:

mod

Encoded on

Plasmid p15B

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Binds the system-specific DNA recognition site 5'-CAGCAC-3'. Necessary for both restriction and methylation (of one of the two A's).
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Contains two different subunits: res and mod. Mod is a homotetramer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	DNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Type III restriction-modification system EcoP15I enzyme mod		PRO_0000088031
Regions
	Region	123 – 126	4	Binding of S-adenosyl methionine Potential

Sequences

	Sequence		Length	Mass (Da)
	P12364 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 0EFE6653BAA3A705	FASTA	645	74,222
	10 20 30 40 50 60 MKKETIFSEV ETANSKQLAV LKANFPQCFD KNGAFIQEKL LEIIRASEVE LSKESYSLNW 70 80 90 100 110 120 LGKSYARLLA NLPPKTLLAE DKTHNQQEEN KNSQHLLIKG DNLEVLKHMV NAYAEKVKMI 130 140 150 160 170 180 YIDPPYNTGK DGFVYNDDRK FTPEQLSELA GIDLDQAKRI LEFTTKGSSS HSAWLTFIYP 190 200 210 220 230 240 RLYIARELMR EDGTIFISID HNEFSQLKLV CDEIFGEQNH VGDLVWKNAT DNNPSNIAVE 250 260 270 280 290 300 HEYIIVYTKN KEQLISEWKS NISDVKNLLV NIGEEFASKY TGNELQEKYT QWFREHRSEL 310 320 330 340 350 360 WPLDRYKYID KDGIYTGSQS VHNPGKEGYR YDIIHPKTKK PCKQQPLMGY RFPLDTMDRL 370 380 390 400 410 420 LSEEKIIFGD DENKIIELKV YAKDYKQKLS SVIHLDGRVA TNELKELFPE MTQPFTNAKT 430 440 450 460 470 480 IKLVEDLISF ACDGEGIVLD FFAGSGTTAH TVFNLNNKNK TSYQFITVQL DEPKKDKSDA 490 500 510 520 530 540 MKHGYNTIFD LTKERLIRAS KKNRDQGFKV YQLMADFRAK DESELTLSNH TFFDDVVLTP 550 560 570 580 590 600 EQYDTLLTTW CLYDGSLLTT PIEDVDLGGY KAHLCDGRLY LIAPNFTSEA LKALLQKVDS 610 620 630 640 DKDFAPNKVV FYGSNFESAK QMELNEALKS YANKKSIELD LVVRN

References

[1]

"Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants." Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A. J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06288 Genomic DNA. Translation: CAA29616.1.
PIR	S03208.
3D structure databases
ProteinModelPortal	P12364.
ModBase	Search...
Protein family/group databases
REBASE	3389. M.EcoP15I.
Proteomic databases
PRIDE	P12364.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PIRSF	PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T3MO_ECOLX

Accession

Primary (citable) accession number: P12364

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program