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P12364

- T3MO_ECOLX

UniProt

P12364 - T3MO_ECOLX

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Protein
Type III restriction-modification system EcoP15I enzyme mod
Gene
mod
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Binds the system-specific DNA recognition site 5'-CAGCAC-3'. Necessary for both restriction and methylation (of one of the two A's).

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

DNA-binding, S-adenosyl-L-methionine

Protein family/group databases

REBASEi3389. M.EcoP15I.

Names & Taxonomyi

Protein namesi
Recommended name:
Type III restriction-modification system EcoP15I enzyme mod (EC:2.1.1.72)
Short name:
M.EcoP15I
Alternative name(s):
EcoP15I methyltransferase
Gene namesi
Name:mod
Encoded oniPlasmid p15B0 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Type III restriction-modification system EcoP15I enzyme mod
PRO_0000088031Add
BLAST

Proteomic databases

PRIDEiP12364.

Interactioni

Subunit structurei

Contains two different subunits: res and mod. Mod is a homotetramer.

Structurei

3D structure databases

ProteinModelPortaliP12364.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1264Binding of S-adenosyl methionine Reviewed prediction

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PIRSFiPIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12364-1 [UniParc]FASTAAdd to Basket

« Hide

MKKETIFSEV ETANSKQLAV LKANFPQCFD KNGAFIQEKL LEIIRASEVE    50
LSKESYSLNW LGKSYARLLA NLPPKTLLAE DKTHNQQEEN KNSQHLLIKG 100
DNLEVLKHMV NAYAEKVKMI YIDPPYNTGK DGFVYNDDRK FTPEQLSELA 150
GIDLDQAKRI LEFTTKGSSS HSAWLTFIYP RLYIARELMR EDGTIFISID 200
HNEFSQLKLV CDEIFGEQNH VGDLVWKNAT DNNPSNIAVE HEYIIVYTKN 250
KEQLISEWKS NISDVKNLLV NIGEEFASKY TGNELQEKYT QWFREHRSEL 300
WPLDRYKYID KDGIYTGSQS VHNPGKEGYR YDIIHPKTKK PCKQQPLMGY 350
RFPLDTMDRL LSEEKIIFGD DENKIIELKV YAKDYKQKLS SVIHLDGRVA 400
TNELKELFPE MTQPFTNAKT IKLVEDLISF ACDGEGIVLD FFAGSGTTAH 450
TVFNLNNKNK TSYQFITVQL DEPKKDKSDA MKHGYNTIFD LTKERLIRAS 500
KKNRDQGFKV YQLMADFRAK DESELTLSNH TFFDDVVLTP EQYDTLLTTW 550
CLYDGSLLTT PIEDVDLGGY KAHLCDGRLY LIAPNFTSEA LKALLQKVDS 600
DKDFAPNKVV FYGSNFESAK QMELNEALKS YANKKSIELD LVVRN 645
Length:645
Mass (Da):74,222
Last modified:October 1, 1989 - v1
Checksum:i0EFE6653BAA3A705
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06288 Genomic DNA. Translation: CAA29616.1.
PIRiS03208.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X06288 Genomic DNA. Translation: CAA29616.1 .
PIRi S03208.

3D structure databases

ProteinModelPortali P12364.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3389. M.EcoP15I.

Proteomic databases

PRIDEi P12364.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PIRSFi PIRSF015855. TypeIII_Mtase_mKpnI. 1 hit.
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 3 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Type III DNA restriction and modification systems EcoP1 and EcoP15. Nucleotide sequence of the EcoP1 operon, the EcoP15 mod gene and some EcoP1 mod mutants."
    Huembelin M., Suri B., Rao D.N., Hornby D.P., Eberle H., Pripfl T., Kenel S., Bickle T.A.
    J. Mol. Biol. 200:23-29(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT3MO_ECOLX
AccessioniPrimary (citable) accession number: P12364
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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