P12355 (PSAF_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photosystem I reaction center subunit III, chloroplastic

Alternative name(s):
Light-harvesting complex I 17 kDa protein
PSI-F

Gene names

Name:

PSAF

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably participates in efficiency of electron transfer from plastocyanin to P700 (or cytochrome c553 in algae and cyanobacteria). This plastocyanin-docking protein contributes to the specific association of plastocyanin to PSI.
Subcellular location	Plastid › chloroplast thylakoid lumen.
Sequence similarities	Belongs to the PsaF family.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Photosystem I Plastid Thylakoid
Domain	Transit peptide
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell photosystem I reaction center Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 77

77

Chloroplast Ref.2

Chain

78 – 231

154

Photosystem I reaction center subunit III, chloroplastic

PRO_0000029346

Secondary structure

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231

Helix Strand Turn

Details...

Beta strand

85 – 87

3

Helix

89 – 96

8

Helix

99 – 101

3

Helix

105 – 107

3

Turn

119 – 122

4

Helix

124 – 127

4

Helix

130 – 133

4

Beta strand

142 – 144

3

Beta strand

151 – 155

5

Turn

158 – 160

3

Helix

162 – 170

9

Turn

171 – 184

14

Beta strand

187 – 189

3

Turn

194 – 196

3

Helix

201 – 203

3

Helix

207 – 210

4

Helix

217 – 220

4

Beta strand

223 – 226

4

Helix

228 – 230

3

Sequences

	Sequence		Length	Mass (Da)
	P12355 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 270E1BAC159D79B4	FASTA	231	25,412
	10 20 30 40 50 60 MSFTIPTNLY KPLATKPKHL SSSSFAPRSK IVCQQENDQQ QPKKLELAKV GANAAAALAL 70 80 90 100 110 120 SSVLLSSWSV APDAAMADIA GLTPCKESKQ FAKREKQALK KLQASLKLYA DDSAPALAIK 130 140 150 160 170 180 ATMEKTKKRF DNYGKYGLLC GSDGLPHLIV SGDQRHWGEF ITPGILFLYI AGWIGWVGRS 190 200 210 220 230 YLIAIRDEKK PTQKEIIIDV PLASSLLFRG FSWPVAAYRE LLNGELVDNN F

References

[1]	"Nucleotide sequence of cDNA clones encoding the entire precursor polypeptides for subunits IV and V of the photosystem I reaction center from spinach." Steppuhn J., Hermans J., Nechushtai R., Ljungberg U., Thuemmler F., Lottspeich F., Herrmann R.G. FEBS Lett. 237:218-224(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of the plastocyanin binding subunit of photosystem I." Hippler M., Ratajczak R., Haehnel W. FEBS Lett. 250:280-284(1989) Cited for: PROTEIN SEQUENCE OF 78-91.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X13133 mRNA. Translation: CAA31523.1.

PIR

F1SP3. S00451.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O01	X-ray	3.40	F	78-231	[»]
2WSC	X-ray	3.30	F	1-231	[»]
2WSE	X-ray	3.49	F	1-231	[»]
2WSF	X-ray	3.48	F	1-231	[»]
3LW5	X-ray	3.30	F	78-231	[»]

ProteinModelPortal

P12355.

SMR

P12355. Positions 78-231.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.8.110. 1 hit.

InterPro

IPR003666. PSI_PsaF.
[Graphical view]

Pfam

PF02507. PSI_PsaF. 1 hit.
[Graphical view]

SUPFAM

SSF81536. PSI_PsaF. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P12355.

Entry information

Entry name

PSAF_SPIOL

Accession

Primary (citable) accession number: P12355

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program