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P12353 (PSAD_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Photosystem I reaction center subunit II, chloroplastic
Alternative name(s):
Photosystem I 20 kDa subunit
Short name=PSI-D
Gene names
Name:psaD
OrganismSpinacia oleracea (Spinach)
Taxonomic identifier3562 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

PsaD can form complexes with ferredoxin and ferredoxin-oxidoreductase in photosystem I (PS I) reaction center. PSAD may encode the ferredoxin-docking protein.

Subcellular location

Plastidchloroplast thylakoid membrane; Peripheral membrane protein; Stromal side Ref.6.

Induction

By light. Ref.3

Sequence similarities

Belongs to the PsaD family.

Ontologies

Keywords
   Biological processPhotosynthesis
   Cellular componentChloroplast
Membrane
Photosystem I
Plastid
Thylakoid
   DomainTransit peptide
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processphotosynthesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentchloroplast thylakoid membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

photosystem I reaction center

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PETFP002212EBI-864919,EBI-864933

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Chloroplast Ref.5
Chain51 – 212162Photosystem I reaction center subunit II, chloroplastic
PRO_0000029377

Regions

Region145 – 1539Ferredoxin and ferredoxin-oxidoreductase binding Potential

Experimental info

Sequence conflict4 – 63ATQ → GTP in CAA54744. Ref.3
Sequence conflict121P → R in CAA54744. Ref.3
Sequence conflict221D → E in CAA54744. Ref.3
Sequence conflict35 – 362VT → LS in CAA32182. Ref.1
Sequence conflict45 – 473HHS → LHT in CAA54744. Ref.3
Sequence conflict581A → R in CAA68728. Ref.2
Sequence conflict60 – 612AA → TP Ref.1
Sequence conflict60 – 612AA → TP Ref.5
Sequence conflict68 – 703PKG → TKA in CAA54744. Ref.3
Sequence conflict911Missing in CAA54744. Ref.3

Secondary structure

............................... 212
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P12353 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: BC5FF64D97A6570E

FASTA21223,103
        10         20         30         40         50         60 
MAMATQATLF SPSSLSSAKP IDTRLTTSFK QPSAVTFASK PASRHHSIRA AAAAEGKAAA 

        70         80         90        100        110        120 
ATETKEAPKG FTPPELDPNT PSPIFAGSTG GLLRKAQVEE FYVITWESPK EQIFEMPTGG 

       130        140        150        160        170        180 
AAIMREGPNL LKLARKEQCL ALGTRLRSKY KIKYQFYRVF PSGEVQYLHP KDGVYPEKVN 

       190        200        210 
PGRQGVGLNM RSIGKNVSPI EVKFTGKQPY DL 

« Hide

References

[1]"Nucleotide sequences of cDNAs encoding the entire precursor polypeptides for subunits II and III of the photosystem I reaction center from spinach."
Muench S., Ljungberg U., Steppuhn J., Schneiderbauer A., Nechushtai R., Beyreuther K., Herrmann R.G.
Curr. Genet. 14:511-518(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Seedling.
[2]"Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide."
Lagoutte B.
FEBS Lett. 232:275-280(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
[3]"Promoter and leader sequences of the spinach PsaD and PsaF genes direct an opposite light response in tobacco cotyledons: PsaD sequences downstream of the ATG codon are required for a positive light response."
Flieger K., Wicke A., Herrmann R.G., Oelmueller R.
Plant J. 6:359-368(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: cv. Monatol.
Tissue: Seedling.
[4]Oelmueller R.
Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[5]"The protein responsible for center A/B in spinach photosystem I: isolation with iron-sulfur cluster(s) and complete sequence analysis."
Oh-oka H., Takahashi Y., Kuriyama K., Saeki K., Matsubara H.
J. Biochem. 103:962-968(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 51-63.
[6]"Purification and membrane topology of PSI-D and PSI-E, two subunits of the photosystem I reaction center."
Lagoutte B., Vallon O.
Eur. J. Biochem. 205:1175-1185(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14017 mRNA. Translation: CAA32182.1.
Y00759 mRNA. Translation: CAA68728.1.
X77674 Genomic DNA. Translation: CAA54744.1.
PIRA1SP2. S03016.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2O01X-ray3.40D75-212[»]
2WSCX-ray3.30D1-212[»]
2WSEX-ray3.49D1-212[»]
2WSFX-ray3.48D1-212[»]
ProteinModelPortalP12353.
SMRP12353. Positions 75-212.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP12353. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.1470.10. 1 hit.
InterProIPR003685. PSI_PsaD.
[Graphical view]
PfamPF02531. PsaD. 1 hit.
[Graphical view]
SUPFAMSSF64234. SSF64234. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP12353.

Entry information

Entry namePSAD_SPIOL
AccessionPrimary (citable) accession number: P12353
Secondary accession number(s): Q43642, Q9S8Z2, Q9S8Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: September 26, 2001
Last modified: February 19, 2014
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references