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P12353

- PSAD_SPIOL

UniProt

P12353 - PSAD_SPIOL

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Protein
Photosystem I reaction center subunit II, chloroplastic
Gene
psaD
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

PsaD can form complexes with ferredoxin and ferredoxin-oxidoreductase in photosystem I (PS I) reaction center. PSAD may encode the ferredoxin-docking protein.

GO - Molecular functioni

  1. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem I reaction center subunit II, chloroplastic
Alternative name(s):
Photosystem I 20 kDa subunit
Short name:
PSI-D
Gene namesi
Name:psaD
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Plastidchloroplast thylakoid membrane; Peripheral membrane protein; Stromal side 1 Publication

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
  2. photosystem I reaction center Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050Chloroplast1 Publication
Add
BLAST
Chaini51 – 212162Photosystem I reaction center subunit II, chloroplastic
PRO_0000029377Add
BLAST

Expressioni

Inductioni

By light.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PETFP002212EBI-864919,EBI-864933

Protein-protein interaction databases

IntActiP12353. 1 interaction.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi89 – 913
Beta strandi97 – 993
Beta strandi103 – 1053
Beta strandi109 – 1113
Turni116 – 1194
Beta strandi130 – 1323
Turni136 – 1394
Turni141 – 1477
Beta strandi148 – 1503
Beta strandi155 – 1573
Turni168 – 1703
Beta strandi171 – 1733
Beta strandi175 – 1773
Beta strandi189 – 1913
Turni194 – 1974
Beta strandi206 – 2083

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O01X-ray3.40D75-212[»]
2WSCX-ray3.30D1-212[»]
2WSEX-ray3.49D1-212[»]
2WSFX-ray3.48D1-212[»]
ProteinModelPortaliP12353.
SMRiP12353. Positions 75-212.

Miscellaneous databases

EvolutionaryTraceiP12353.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni145 – 1539Ferredoxin and ferredoxin-oxidoreductase binding Reviewed prediction

Sequence similaritiesi

Belongs to the PsaD family.

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.1470.10. 1 hit.
InterProiIPR003685. PSI_PsaD.
[Graphical view]
PfamiPF02531. PsaD. 1 hit.
[Graphical view]
SUPFAMiSSF64234. SSF64234. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12353-1 [UniParc]FASTAAdd to Basket

« Hide

MAMATQATLF SPSSLSSAKP IDTRLTTSFK QPSAVTFASK PASRHHSIRA    50
AAAAEGKAAA ATETKEAPKG FTPPELDPNT PSPIFAGSTG GLLRKAQVEE 100
FYVITWESPK EQIFEMPTGG AAIMREGPNL LKLARKEQCL ALGTRLRSKY 150
KIKYQFYRVF PSGEVQYLHP KDGVYPEKVN PGRQGVGLNM RSIGKNVSPI 200
EVKFTGKQPY DL 212
Length:212
Mass (Da):23,103
Last modified:September 26, 2001 - v2
Checksum:iBC5FF64D97A6570E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 63ATQ → GTP in CAA54744. 1 Publication
Sequence conflicti12 – 121P → R in CAA54744. 1 Publication
Sequence conflicti22 – 221D → E in CAA54744. 1 Publication
Sequence conflicti35 – 362VT → LS in CAA32182. 1 Publication
Sequence conflicti45 – 473HHS → LHT in CAA54744. 1 Publication
Sequence conflicti58 – 581A → R in CAA68728. 1 Publication
Sequence conflicti60 – 612AA → TP1 Publication
Sequence conflicti60 – 612AA → TP1 Publication
Sequence conflicti68 – 703PKG → TKA in CAA54744. 1 Publication
Sequence conflicti91 – 911Missing in CAA54744. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14017 mRNA. Translation: CAA32182.1.
Y00759 mRNA. Translation: CAA68728.1.
X77674 Genomic DNA. Translation: CAA54744.1.
PIRiS03016. A1SP2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14017 mRNA. Translation: CAA32182.1 .
Y00759 mRNA. Translation: CAA68728.1 .
X77674 Genomic DNA. Translation: CAA54744.1 .
PIRi S03016. A1SP2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2O01 X-ray 3.40 D 75-212 [» ]
2WSC X-ray 3.30 D 1-212 [» ]
2WSE X-ray 3.49 D 1-212 [» ]
2WSF X-ray 3.48 D 1-212 [» ]
ProteinModelPortali P12353.
SMRi P12353. Positions 75-212.
ModBasei Search...

Protein-protein interaction databases

IntActi P12353. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P12353.

Family and domain databases

Gene3Di 3.30.1470.10. 1 hit.
InterProi IPR003685. PSI_PsaD.
[Graphical view ]
Pfami PF02531. PsaD. 1 hit.
[Graphical view ]
SUPFAMi SSF64234. SSF64234. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of cDNAs encoding the entire precursor polypeptides for subunits II and III of the photosystem I reaction center from spinach."
    Muench S., Ljungberg U., Steppuhn J., Schneiderbauer A., Nechushtai R., Beyreuther K., Herrmann R.G.
    Curr. Genet. 14:511-518(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Seedling.
  2. "Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide."
    Lagoutte B.
    FEBS Lett. 232:275-280(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  3. "Promoter and leader sequences of the spinach PsaD and PsaF genes direct an opposite light response in tobacco cotyledons: PsaD sequences downstream of the ATG codon are required for a positive light response."
    Flieger K., Wicke A., Herrmann R.G., Oelmueller R.
    Plant J. 6:359-368(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: cv. Monatol.
    Tissue: Seedling.
  4. Oelmueller R.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. "The protein responsible for center A/B in spinach photosystem I: isolation with iron-sulfur cluster(s) and complete sequence analysis."
    Oh-oka H., Takahashi Y., Kuriyama K., Saeki K., Matsubara H.
    J. Biochem. 103:962-968(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 51-63.
  6. "Purification and membrane topology of PSI-D and PSI-E, two subunits of the photosystem I reaction center."
    Lagoutte B., Vallon O.
    Eur. J. Biochem. 205:1175-1185(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPSAD_SPIOL
AccessioniPrimary (citable) accession number: P12353
Secondary accession number(s): Q43642, Q9S8Z2, Q9S8Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: September 26, 2001
Last modified: June 11, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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