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P12353

- PSAD_SPIOL

UniProt

P12353 - PSAD_SPIOL

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Protein

Photosystem I reaction center subunit II, chloroplastic

Gene

psaD

Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

PsaD can form complexes with ferredoxin and ferredoxin-oxidoreductase in photosystem I (PS I) reaction center. PSAD may encode the ferredoxin-docking protein.

GO - Biological processi

  1. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem I reaction center subunit II, chloroplastic
Alternative name(s):
Photosystem I 20 kDa subunit
Short name:
PSI-D
Gene namesi
Name:psaD
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Plastidchloroplast thylakoid membrane 1 Publication; Peripheral membrane protein 1 Publication; Stromal side 1 Publication

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
  2. photosystem I reaction center Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050Chloroplast1 PublicationAdd
BLAST
Chaini51 – 212162Photosystem I reaction center subunit II, chloroplasticPRO_0000029377Add
BLAST

Expressioni

Inductioni

By light.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PETFP002212EBI-864919,EBI-864933

Protein-protein interaction databases

IntActiP12353. 1 interaction.

Structurei

Secondary structure

1
212
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi89 – 913Combined sources
Beta strandi97 – 993Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi109 – 1113Combined sources
Turni116 – 1194Combined sources
Beta strandi130 – 1323Combined sources
Turni136 – 1394Combined sources
Turni141 – 1477Combined sources
Beta strandi148 – 1503Combined sources
Beta strandi155 – 1573Combined sources
Turni168 – 1703Combined sources
Beta strandi171 – 1733Combined sources
Beta strandi175 – 1773Combined sources
Beta strandi189 – 1913Combined sources
Turni194 – 1974Combined sources
Beta strandi206 – 2083Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2O01X-ray3.40D75-212[»]
2WSCX-ray3.30D1-212[»]
2WSEX-ray3.49D1-212[»]
2WSFX-ray3.48D1-212[»]
ProteinModelPortaliP12353.
SMRiP12353. Positions 75-212.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12353.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni145 – 1539Ferredoxin and ferredoxin-oxidoreductase bindingSequence Analysis

Sequence similaritiesi

Belongs to the PsaD family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.1470.10. 1 hit.
InterProiIPR003685. PSI_PsaD.
[Graphical view]
PfamiPF02531. PsaD. 1 hit.
[Graphical view]
SUPFAMiSSF64234. SSF64234. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12353-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMATQATLF SPSSLSSAKP IDTRLTTSFK QPSAVTFASK PASRHHSIRA
60 70 80 90 100
AAAAEGKAAA ATETKEAPKG FTPPELDPNT PSPIFAGSTG GLLRKAQVEE
110 120 130 140 150
FYVITWESPK EQIFEMPTGG AAIMREGPNL LKLARKEQCL ALGTRLRSKY
160 170 180 190 200
KIKYQFYRVF PSGEVQYLHP KDGVYPEKVN PGRQGVGLNM RSIGKNVSPI
210
EVKFTGKQPY DL
Length:212
Mass (Da):23,103
Last modified:September 26, 2001 - v2
Checksum:iBC5FF64D97A6570E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 63ATQ → GTP in CAA54744. (PubMed:7920722)Curated
Sequence conflicti12 – 121P → R in CAA54744. (PubMed:7920722)Curated
Sequence conflicti22 – 221D → E in CAA54744. (PubMed:7920722)Curated
Sequence conflicti35 – 362VT → LS in CAA32182. (PubMed:3066511)Curated
Sequence conflicti45 – 473HHS → LHT in CAA54744. (PubMed:7920722)Curated
Sequence conflicti58 – 581A → R in CAA68728. (PubMed:3288500)Curated
Sequence conflicti60 – 612AA → TP(PubMed:3066511)Curated
Sequence conflicti60 – 612AA → TP(PubMed:3049567)Curated
Sequence conflicti68 – 703PKG → TKA in CAA54744. (PubMed:7920722)Curated
Sequence conflicti91 – 911Missing in CAA54744. (PubMed:7920722)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14017 mRNA. Translation: CAA32182.1.
Y00759 mRNA. Translation: CAA68728.1.
X77674 Genomic DNA. Translation: CAA54744.1.
PIRiS03016. A1SP2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14017 mRNA. Translation: CAA32182.1 .
Y00759 mRNA. Translation: CAA68728.1 .
X77674 Genomic DNA. Translation: CAA54744.1 .
PIRi S03016. A1SP2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2O01 X-ray 3.40 D 75-212 [» ]
2WSC X-ray 3.30 D 1-212 [» ]
2WSE X-ray 3.49 D 1-212 [» ]
2WSF X-ray 3.48 D 1-212 [» ]
ProteinModelPortali P12353.
SMRi P12353. Positions 75-212.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P12353. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P12353.

Family and domain databases

Gene3Di 3.30.1470.10. 1 hit.
InterProi IPR003685. PSI_PsaD.
[Graphical view ]
Pfami PF02531. PsaD. 1 hit.
[Graphical view ]
SUPFAMi SSF64234. SSF64234. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of cDNAs encoding the entire precursor polypeptides for subunits II and III of the photosystem I reaction center from spinach."
    Muench S., Ljungberg U., Steppuhn J., Schneiderbauer A., Nechushtai R., Beyreuther K., Herrmann R.G.
    Curr. Genet. 14:511-518(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Seedling.
  2. "Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide."
    Lagoutte B.
    FEBS Lett. 232:275-280(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Leaf.
  3. "Promoter and leader sequences of the spinach PsaD and PsaF genes direct an opposite light response in tobacco cotyledons: PsaD sequences downstream of the ATG codon are required for a positive light response."
    Flieger K., Wicke A., Herrmann R.G., Oelmueller R.
    Plant J. 6:359-368(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
    Strain: cv. Monatol.
    Tissue: Seedling.
  4. Oelmueller R.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  5. "The protein responsible for center A/B in spinach photosystem I: isolation with iron-sulfur cluster(s) and complete sequence analysis."
    Oh-oka H., Takahashi Y., Kuriyama K., Saeki K., Matsubara H.
    J. Biochem. 103:962-968(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 51-63.
  6. "Purification and membrane topology of PSI-D and PSI-E, two subunits of the photosystem I reaction center."
    Lagoutte B., Vallon O.
    Eur. J. Biochem. 205:1175-1185(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPSAD_SPIOL
AccessioniPrimary (citable) accession number: P12353
Secondary accession number(s): Q43642, Q9S8Z2, Q9S8Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: September 26, 2001
Last modified: November 26, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3