P12353 (PSAD_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Photosystem I reaction center subunit II, chloroplastic

Alternative name(s):
Photosystem I 20 kDa subunit
Short name=PSI-D

Gene names

Name:

psaD

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PsaD can form complexes with ferredoxin and ferredoxin-oxidoreductase in photosystem I (PS I) reaction center. PSAD may encode the ferredoxin-docking protein.
Subcellular location	Plastid › chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side Ref.6.
Induction	By light. Ref.3
Sequence similarities	Belongs to the PsaD family.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem I Plastid Thylakoid
Domain	Transit peptide
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell photosystem I reaction center Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PETF	P00221	2	EBI-864919,EBI-864933

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 50

50

Chloroplast Ref.5

Chain

51 – 212

162

Photosystem I reaction center subunit II, chloroplastic

PRO_0000029377

Regions

Region

145 – 153

9

Ferredoxin and ferredoxin-oxidoreductase binding Potential

Experimental info

Sequence conflict

4 – 6

3

ATQ → GTP in CAA54744. Ref.3

Sequence conflict

12

1

P → R in CAA54744. Ref.3

Sequence conflict

22

1

D → E in CAA54744. Ref.3

Sequence conflict

35 – 36

2

VT → LS in CAA32182. Ref.1

Sequence conflict

45 – 47

3

HHS → LHT in CAA54744. Ref.3

Sequence conflict

58

1

A → R in CAA68728. Ref.2

Sequence conflict

60 – 61

2

AA → TP Ref.1

Sequence conflict

60 – 61

2

AA → TP Ref.5

Sequence conflict

68 – 70

3

PKG → TKA in CAA54744. Ref.3

Sequence conflict

91

1

Missing in CAA54744. Ref.3

Secondary structure

1

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212

Helix Strand Turn

Details...

Beta strand

89 – 91

3

Beta strand

97 – 99

3

Beta strand

103 – 105

3

Beta strand

109 – 111

3

Turn

116 – 119

4

Beta strand

130 – 132

3

Turn

136 – 139

4

Turn

141 – 147

7

Beta strand

148 – 150

3

Beta strand

155 – 157

3

Turn

168 – 170

3

Beta strand

171 – 173

3

Beta strand

175 – 177

3

Beta strand

189 – 191

3

Turn

194 – 197

4

Beta strand

206 – 208

3

Sequences

	Sequence		Length	Mass (Da)
	P12353 [UniParc]. Last modified September 26, 2001. Version 2. Checksum: BC5FF64D97A6570E	FASTA	212	23,103
	10 20 30 40 50 60 MAMATQATLF SPSSLSSAKP IDTRLTTSFK QPSAVTFASK PASRHHSIRA AAAAEGKAAA 70 80 90 100 110 120 ATETKEAPKG FTPPELDPNT PSPIFAGSTG GLLRKAQVEE FYVITWESPK EQIFEMPTGG 130 140 150 160 170 180 AAIMREGPNL LKLARKEQCL ALGTRLRSKY KIKYQFYRVF PSGEVQYLHP KDGVYPEKVN 190 200 210 PGRQGVGLNM RSIGKNVSPI EVKFTGKQPY DL

References

[1]	"Nucleotide sequences of cDNAs encoding the entire precursor polypeptides for subunits II and III of the photosystem I reaction center from spinach." Muench S., Ljungberg U., Steppuhn J., Schneiderbauer A., Nechushtai R., Beyreuther K., Herrmann R.G. Curr. Genet. 14:511-518(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Seedling.
[2]	"Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I polypeptide." Lagoutte B. FEBS Lett. 232:275-280(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[3]	"Promoter and leader sequences of the spinach PsaD and PsaF genes direct an opposite light response in tobacco cotyledons: PsaD sequences downstream of the ATG codon are required for a positive light response." Flieger K., Wicke A., Herrmann R.G., Oelmueller R. Plant J. 6:359-368(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: cv. Monatol. Tissue: Seedling.
[4]	Oelmueller R. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[5]	"The protein responsible for center A/B in spinach photosystem I: isolation with iron-sulfur cluster(s) and complete sequence analysis." Oh-oka H., Takahashi Y., Kuriyama K., Saeki K., Matsubara H. J. Biochem. 103:962-968(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 51-63.
[6]	"Purification and membrane topology of PSI-D and PSI-E, two subunits of the photosystem I reaction center." Lagoutte B., Vallon O. Eur. J. Biochem. 205:1175-1185(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X14017 mRNA. Translation: CAA32182.1.
Y00759 mRNA. Translation: CAA68728.1.
X77674 Genomic DNA. Translation: CAA54744.1.

PIR

A1SP2. S03016.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2O01	X-ray	3.40	D	75-212	[»]
2WSC	X-ray	3.30	D	1-212	[»]
2WSE	X-ray	3.49	D	1-212	[»]
2WSF	X-ray	3.48	D	1-212	[»]

ProteinModelPortal

P12353.

SMR

P12353. Positions 75-212.

ModBase

Search...

Protein-protein interaction databases

IntAct

P12353. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.1470.10. 1 hit.

InterPro

IPR003685. PSI_PsaD.
[Graphical view]

Pfam

PF02531. PsaD. 1 hit.
[Graphical view]

SUPFAM

SSF64234. PsaD. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P12353.

Entry information

Entry name

PSAD_SPIOL

Accession

Primary (citable) accession number: P12353
Secondary accession number(s): Q43642, Q9S8Z2, Q9S8Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	September 26, 2001
Last modified:	April 3, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program