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Protein

Aspartate aminotransferase, mitochondrial

Gene

GOT2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids (By similarity).By similarity

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processLipid transport, Transport
LigandPyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, mitochondrial (EC:2.6.1.1)
Short name:
mAspAT
Alternative name(s):
Fatty acid-binding protein
Short name:
FABP-1
Glutamate oxaloacetate transaminase 2
Kynurenine aminotransferase 4
Kynurenine aminotransferase IV
Kynurenine--oxoglutarate transaminase 4
Kynurenine--oxoglutarate transaminase IV
Plasma membrane-associated fatty acid-binding protein
Short name:
FABPpm
Transaminase A
Gene namesi
Name:GOT2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Organism-specific databases

VGNCiVGNC:29503 GOT2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29Mitochondrion1 PublicationAdd BLAST29
ChainiPRO_000000121430 – 430Aspartate aminotransferase, mitochondrialAdd BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphothreonineBy similarity1
Modified residuei59N6-acetyllysineBy similarity1
Modified residuei73N6-acetyllysine; alternateBy similarity1
Modified residuei73N6-succinyllysine; alternateBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei90N6-acetyllysine; alternateBy similarity1
Modified residuei90N6-succinyllysine; alternateBy similarity1
Modified residuei96Nitrated tyrosine; alternateBy similarity1
Modified residuei96Phosphotyrosine; alternateBy similarity1
Modified residuei107N6-acetyllysine; alternateBy similarity1
Modified residuei107N6-succinyllysine; alternateBy similarity1
Modified residuei122N6-acetyllysine; alternateBy similarity1
Modified residuei122N6-succinyllysine; alternateBy similarity1
Modified residuei143PhosphoserineBy similarity1
Modified residuei159N6-acetyllysine; alternateBy similarity1
Modified residuei159N6-succinyllysine; alternateBy similarity1
Modified residuei185N6-acetyllysine; alternateBy similarity1
Modified residuei185N6-succinyllysine; alternateBy similarity1
Modified residuei227N6-succinyllysineBy similarity1
Modified residuei234N6-acetyllysineBy similarity1
Modified residuei279N6-(pyridoxal phosphate)lysine; alternate1
Modified residuei279N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-succinyllysine; alternateBy similarity1
Modified residuei302N6-acetyllysineBy similarity1
Modified residuei309N6-acetyllysine; alternateBy similarity1
Modified residuei309N6-succinyllysine; alternate1 Publication1
Modified residuei313Asymmetric dimethylarginineBy similarity1
Modified residuei338N6-acetyllysine; alternateBy similarity1
Modified residuei338N6-succinyllysine; alternateBy similarity1
Modified residuei345N6-acetyllysineBy similarity1
Modified residuei363N6-acetyllysine; alternateBy similarity1
Modified residuei363N6-succinyllysine; alternateBy similarity1
Modified residuei364N6-acetyllysineBy similarity1
Modified residuei387N6-acetyllysineBy similarity1
Modified residuei396N6-acetyllysine; alternateBy similarity1
Modified residuei396N6-succinyllysine; alternateBy similarity1
Modified residuei404N6-acetyllysine; alternateBy similarity1
Modified residuei404N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiP12344
PeptideAtlasiP12344
PRIDEiP12344

Expressioni

Gene expression databases

BgeeiENSBTAG00000007172

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP12344, 1 interactor
STRINGi9913.ENSBTAP00000009440

Structurei

3D structure databases

ProteinModelPortaliP12344
SMRiP12344
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1411 Eukaryota
COG1448 LUCA
GeneTreeiENSGT00390000014081
HOGENOMiHOG000185898
HOVERGENiHBG000951
InParanoidiP12344
KOiK14455
OMAiRVGACTI
OrthoDBiEOG091G06G3
TreeFamiTF300641

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR000796 Asp_trans
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11879 PTHR11879, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PRINTSiPR00799 TRANSAMINASE
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLHSGRFL SGVAAAFHPG LAAAASARAS SWWAHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK NLDKEYLPIA
110 120 130 140 150
GLAEFCKASA ELALGENNEV LKSGRYVTVQ TISGTGALRI GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP TWGNHTPIFR DAGMQLQSYR YYDPKTCGFD FTGAIEDISK
210 220 230 240 250
IPAQSVILLH ACAHNPTGVD PRPEQWKEMA TVVKKNNLFA FFDMAYQGFA
260 270 280 290 300
SGDGNKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE
310 320 330 340 350
AKRVESQLKI LIRPMYSNPP INGARIASTI LTSPDLRKQW LHEVKGMADR
360 370 380 390 400
IISMRTQLVS NLKKEGSSHN WQHIIDQIGM FCYTGLKPEQ VERLTKEFSI
410 420 430
YMTKDGRISV AGVTSGNVAY LAHAIHQVTK
Length:430
Mass (Da):47,514
Last modified:November 1, 1995 - v2
Checksum:i16DDF475382035AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25466 mRNA Translation: CAA80960.1
BT030486 mRNA Translation: ABQ12926.1
BC102303 mRNA Translation: AAI02304.1
PIRiS35960
RefSeqiNP_777231.1, NM_174806.2
UniGeneiBt.1316

Genome annotation databases

EnsembliENSBTAT00000009440; ENSBTAP00000009440; ENSBTAG00000007172
GeneIDi286886
KEGGibta:286886

Similar proteinsi

Entry informationi

Entry nameiAATM_BOVIN
AccessioniPrimary (citable) accession number: P12344
Secondary accession number(s): A5D967, Q3T0Q1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health