Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chlorophyll a-b binding protein, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi59 – 591Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygen2 Publications
Binding sitei81 – 811Chlorophyll-a 1; via amide nitrogen
Binding sitei87 – 871Chlorophyll-a 1
Metal bindingi100 – 1001Magnesium (chlorophyll-a 1 axial ligand)2 Publications
Metal bindingi103 – 1031Magnesium (chlorophyll-a 2 axial ligand)2 Publications
Binding sitei105 – 1051Chlorophyll-b 2
Binding sitei138 – 1381Chlorophyll-a 3
Binding sitei148 – 1481Chlorophyll-a 3; via amide nitrogen
Metal bindingi154 – 1541Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygen2 Publications
Binding sitei158 – 1581Chlorophyll-b 3
Binding sitei166 – 1661Chlorophyll-b 4 or chlorophyll-b 5
Metal bindingi174 – 1741Magnesium (chlorophyll-b 3 axial ligand)2 Publications
Binding sitei177 – 1771Chlorophyll-b 4
Binding sitei183 – 1831Chlorophyll-b 2; via amide nitrogen
Binding sitei214 – 2141Chlorophyll-a 5
Metal bindingi215 – 2151Magnesium (chlorophyll-a 3 axial ligand)2 Publications
Metal bindingi218 – 2181Magnesium (chlorophyll-a 4 axial ligand)2 Publications
Binding sitei220 – 2201Chlorophyll-a 1
Metal bindingi232 – 2321Magnesium (chlorophyll-a 5 axial ligand)2 Publications
Metal bindingi247 – 2471Magnesium (chlorophyll-a 6 axial ligand)2 Publications
Binding sitei256 – 2561Chlorophyll-a 6; via amide nitrogen
Binding sitei263 – 2631Chlorophyll-b 5; via carbonyl oxygen

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Chlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein, chloroplastic
Alternative name(s):
LHCII type I CAB
Short name:
LHCP
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei101 – 12121HelicalSequence analysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Transmembranei221 – 24121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Photosystem II, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535Chloroplast1 PublicationAdd
BLAST
Chaini36 – 267232Chlorophyll a-b binding protein, chloroplasticPRO_0000003700Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361N2-acetylarginine1 Publication
Modified residuei38 – 381Phosphothreonine1 Publication

Post-translational modificationi

Photoregulated by reversible phosphorylation of its threonine residues.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP12333.

PTM databases

iPTMnetiP12333.

Interactioni

Subunit structurei

The LHC complex consists of chlorophyll a-b binding proteins.1 Publication

Structurei

Secondary structure

1
267
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi51 – 533Combined sources
Helixi61 – 633Combined sources
Helixi90 – 12132Combined sources
Turni122 – 1243Combined sources
Turni132 – 1343Combined sources
Helixi135 – 1406Combined sources
Helixi147 – 1493Combined sources
Helixi159 – 17921Combined sources
Beta strandi187 – 1904Combined sources
Helixi194 – 1963Combined sources
Helixi205 – 23632Combined sources
Helixi240 – 24910Combined sources
Turni251 – 2533Combined sources
Helixi256 – 2594Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RWTX-ray2.72A/B/C/D/E/F/G/H/I/J36-267[»]
3JCUelectron microscopy3.20G/N/Y/g/n/y1-267[»]
4LCZX-ray2.60A/B/C44-267[»]
ProteinModelPortaliP12333.
SMRiP12333. Positions 49-267.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12333.

Family & Domainsi

Domaini

The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSTMALSS PSLAGKAVKL GPTASEIIGE GRITMRKTAG KPKTVQSSSP
60 70 80 90 100
WYGPDRVKYL GPFSGESPSY LTGEFPGDYG WDTAGLSADP ETFAKNRELE
110 120 130 140 150
VIHCRWAMLG ALGCVFPELL ARNGVKFGEA VWFKAGSQIF SEGGLDYLGN
160 170 180 190 200
PSLVHAQSIL AIWACQVILM GAVEGYRIAG GPLGEVVDPL YPGGSFDPLG
210 220 230 240 250
LADDPEAFAE LKVKEIKNGR LAMFSMFGFF VQAIVTGKGP LENLADHLAD
260
PVNNNAWNFA TNFVPGK
Length:267
Mass (Da):28,424
Last modified:October 1, 1989 - v1
Checksum:i23474EBDA23F6545
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14341 mRNA. Translation: CAA32526.1.
PIRiJQ0020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14341 mRNA. Translation: CAA32526.1.
PIRiJQ0020.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RWTX-ray2.72A/B/C/D/E/F/G/H/I/J36-267[»]
3JCUelectron microscopy3.20G/N/Y/g/n/y1-267[»]
4LCZX-ray2.60A/B/C44-267[»]
ProteinModelPortaliP12333.
SMRiP12333. Positions 49-267.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP12333.

Proteomic databases

PRIDEiP12333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12333.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCB2A_SPIOL
AccessioniPrimary (citable) accession number: P12333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: July 6, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.