P12323 (KLK2_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glandular kallikrein, prostatic EC=3.4.21.35 Alternative name(s): Prostate esterase Tissue kallikrein
Organism	Cavia porcellus (Guinea pig)
Taxonomic identifier	10141 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Glandular kallikrein, prostatic		PRO_0000088701
Regions
	Domain	1 – 236	236	Peptidase S1
Sites
	Active site	41	1	Charge relay system
	Active site	96	1	Charge relay system
	Active site	191	1	Charge relay system
Amino acid modifications
	Glycosylation	78	1	N-linked (GlcNAc...)
	Glycosylation	169	1	N-linked (GlcNAc...)
	Disulfide bond	7 ↔ 151		By similarity
	Disulfide bond	26 ↔ 42		By similarity
	Disulfide bond	128 ↔ 197		By similarity
	Disulfide bond	162 ↔ 176		By similarity
	Disulfide bond	187 ↔ 212		By similarity
Natural variations
	Natural variant	50	1	K → W.

Sequences

	Sequence		Length	Mass (Da)
	P12323 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 56DC81BC10D49A64	FASTA	239	25,989
	10 20 30 40 50 60 VIGGQECARD SHPWQAAVYY YSDIKCGGVL VDPQWVLTAA HCINDSNQVK LGRHNLFEDE 70 80 90 100 110 120 DTAQHFLVSQ SVPHPDFNMS LLEPHNVLPN EDYSHDLMLL RLNQPAQITD SVQVMPLPTQ 130 140 150 160 170 180 EVQVGTTCRA LGWGSIDPDP AHPVFPDELQ CVGLEILPSK NCDDAHIANV TGTMLCAGDL 190 200 210 220 230 AGGKDTCVGD SGGPLICDGV LQGLTSWGDS PCGVAHSPSL YTKVIEYREW IERTMADNP

References

[1]	"Amino acid sequence of guinea pig prostate kallikrein." Dunbar J.C., Bradshaw R.A. Biochemistry 26:3471-3478(1987) [PubMed: 3307909] [Abstract] Cited for: PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	A27207.
3D structure databases
ProteinModelPortal	P12323.
SMR	P12323. Positions 1-238.
ModBase	Search...
Protein family/group databases
MEROPS	S01.160.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000005699; ENSCPOP00000005082; ENSCPOG00000005638.
Phylogenomic databases
eggNOG	roNOG16199.
GeneTree	ENSGT00590000082811.
HOVERGEN	HBG013304.
Family and domain databases
InterPro	IPR009003. Pept_cys/ser_Trypsin-like. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KLK2_CAVPO

Accession

Primary (citable) accession number: P12323

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 16, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program