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Protein

Uracil-DNA glycosylase

Gene

ung

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Catalytic activityi

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Proton acceptor

GO - Molecular functioni

  • uracil DNA N-glycosylase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciEcoCyc:EG11058-MONOMER.
ECOL316407:JW2564-MONOMER.
MetaCyc:EG11058-MONOMER.
BRENDAi3.2.2.27. 2026.
SABIO-RKP12295.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil-DNA glycosylase (EC:3.2.2.27)
Short name:
UDG
Gene namesi
Name:ung
Ordered Locus Names:b2580, JW2564
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11058. ung.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 229228Uracil-DNA glycosylasePRO_0000176091Add
BLAST

Proteomic databases

PaxDbiP12295.
PRIDEiP12295.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
ndkP0A7633EBI-559403,EBI-370139
UGIP147394EBI-559403,EBI-1025973From a different organism.

Protein-protein interaction databases

BioGridi4263474. 132 interactions.
DIPiDIP-11092N.
IntActiP12295. 10 interactions.
MINTiMINT-194643.
STRINGi511145.b2580.

Structurei

Secondary structure

1
229
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 115Combined sources
Helixi14 – 163Combined sources
Helixi18 – 3316Combined sources
Beta strandi37 – 393Combined sources
Helixi41 – 433Combined sources
Helixi46 – 505Combined sources
Helixi53 – 553Combined sources
Beta strandi58 – 647Combined sources
Turni69 – 713Combined sources
Beta strandi74 – 774Combined sources
Beta strandi81 – 833Combined sources
Helixi87 – 9913Combined sources
Helixi112 – 1154Combined sources
Turni116 – 1183Combined sources
Beta strandi119 – 1257Combined sources
Turni133 – 1386Combined sources
Helixi141 – 15515Combined sources
Beta strandi160 – 1656Combined sources
Helixi166 – 1716Combined sources
Turni172 – 1743Combined sources
Turni177 – 1793Combined sources
Beta strandi180 – 1856Combined sources
Turni190 – 19910Combined sources
Helixi202 – 21211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUGX-ray1.60A1-229[»]
1EUIX-ray3.20A/B2-229[»]
1FLZX-ray2.30A2-229[»]
1LQGX-ray2.90A/B2-229[»]
1LQJX-ray3.35A/B/C/D2-229[»]
1LQMX-ray3.20A/C/E/G2-229[»]
1UUGX-ray2.40A/C1-229[»]
2EUGX-ray1.50A1-229[»]
2UUGX-ray2.60A/B1-229[»]
3EUGX-ray1.43A1-229[»]
3UF7X-ray1.20A1-229[»]
4EUGX-ray1.40A1-229[»]
5EUGX-ray1.60A1-229[»]
ProteinModelPortaliP12295.
SMRiP12295. Positions 3-225.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12295.

Family & Domainsi

Sequence similaritiesi

Belongs to the uracil-DNA glycosylase family.Curated

Phylogenomic databases

eggNOGiENOG4105D5S. Bacteria.
COG0692. LUCA.
HOGENOMiHOG000229528.
InParanoidiP12295.
KOiK03648.
OMAiIALIPKN.
PhylomeDBiP12295.

Family and domain databases

CDDicd10027. UDG_F1. 1 hit.
Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_00148. UDG. 1 hit.
InterProiIPR002043. UDG_fam1.
IPR018085. Ura-DNA_Glyclase_AS.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERiPTHR11264. PTHR11264. 1 hit.
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SMARTiSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
TIGRFAMsiTIGR00628. ung. 1 hit.
PROSITEiPS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANELTWHDV LAEEKQQPYF LNTLQTVASE RQSGVTIYPP QKDVFNAFRF
60 70 80 90 100
TELGDVKVVI LGQDPYHGPG QAHGLAFSVR PGIAIPPSLL NMYKELENTI
110 120 130 140 150
PGFTRPNHGY LESWARQGVL LLNTVLTVRA GQAHSHASLG WETFTDKVIS
160 170 180 190 200
LINQHREGVV FLLWGSHAQK KGAIIDKQRH HVLKAPHPSP LSAHRGFFGC
210 220
NHFVLANQWL EQRGETPIDW MPVLPAESE
Length:229
Mass (Da):25,693
Last modified:January 23, 2007 - v2
Checksum:iCD44F1E214FE74ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03725 Genomic DNA. Translation: AAA24743.1.
D13169 Genomic DNA. Translation: BAA02448.1.
D64044 Genomic DNA. Translation: BAA10923.1.
U00096 Genomic DNA. Translation: AAC75633.1.
AP009048 Genomic DNA. Translation: BAA16466.1.
PIRiA28175. DGECU.
RefSeqiNP_417075.1. NC_000913.3.
WP_001262716.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75633; AAC75633; b2580.
BAA16466; BAA16466; BAA16466.
GeneIDi947067.
KEGGiecj:JW2564.
eco:b2580.
PATRICi32120561. VBIEscCol129921_2683.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03725 Genomic DNA. Translation: AAA24743.1.
D13169 Genomic DNA. Translation: BAA02448.1.
D64044 Genomic DNA. Translation: BAA10923.1.
U00096 Genomic DNA. Translation: AAC75633.1.
AP009048 Genomic DNA. Translation: BAA16466.1.
PIRiA28175. DGECU.
RefSeqiNP_417075.1. NC_000913.3.
WP_001262716.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUGX-ray1.60A1-229[»]
1EUIX-ray3.20A/B2-229[»]
1FLZX-ray2.30A2-229[»]
1LQGX-ray2.90A/B2-229[»]
1LQJX-ray3.35A/B/C/D2-229[»]
1LQMX-ray3.20A/C/E/G2-229[»]
1UUGX-ray2.40A/C1-229[»]
2EUGX-ray1.50A1-229[»]
2UUGX-ray2.60A/B1-229[»]
3EUGX-ray1.43A1-229[»]
3UF7X-ray1.20A1-229[»]
4EUGX-ray1.40A1-229[»]
5EUGX-ray1.60A1-229[»]
ProteinModelPortaliP12295.
SMRiP12295. Positions 3-225.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263474. 132 interactions.
DIPiDIP-11092N.
IntActiP12295. 10 interactions.
MINTiMINT-194643.
STRINGi511145.b2580.

Proteomic databases

PaxDbiP12295.
PRIDEiP12295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75633; AAC75633; b2580.
BAA16466; BAA16466; BAA16466.
GeneIDi947067.
KEGGiecj:JW2564.
eco:b2580.
PATRICi32120561. VBIEscCol129921_2683.

Organism-specific databases

EchoBASEiEB1051.
EcoGeneiEG11058. ung.

Phylogenomic databases

eggNOGiENOG4105D5S. Bacteria.
COG0692. LUCA.
HOGENOMiHOG000229528.
InParanoidiP12295.
KOiK03648.
OMAiIALIPKN.
PhylomeDBiP12295.

Enzyme and pathway databases

BioCyciEcoCyc:EG11058-MONOMER.
ECOL316407:JW2564-MONOMER.
MetaCyc:EG11058-MONOMER.
BRENDAi3.2.2.27. 2026.
SABIO-RKP12295.

Miscellaneous databases

EvolutionaryTraceiP12295.
PROiP12295.

Family and domain databases

CDDicd10027. UDG_F1. 1 hit.
Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_00148. UDG. 1 hit.
InterProiIPR002043. UDG_fam1.
IPR018085. Ura-DNA_Glyclase_AS.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERiPTHR11264. PTHR11264. 1 hit.
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SMARTiSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
TIGRFAMsiTIGR00628. ung. 1 hit.
PROSITEiPS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUNG_ECOLI
AccessioniPrimary (citable) accession number: P12295
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.