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Protein

Regulatory protein BlaR1

Gene

blaR1

Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

BlaR1 is a potential penicillin-binding protein required for induction of beta-lactamase in Bacillus licheniformis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei402 – 4021Acyl-ester intermediateBy similarity

GO - Molecular functioni

Complete GO annotation...

Protein family/group databases

MEROPSiM56.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein BlaR1
Gene namesi
Name:blaR1
Synonyms:penR1
OrganismiBacillus licheniformis
Taxonomic identifieri1402 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44CytoplasmicSequence analysis
Transmembranei5 – 2420HelicalSequence analysisAdd
BLAST
Topological domaini25 – 4016ExtracellularSequence analysisAdd
BLAST
Transmembranei41 – 6020HelicalSequence analysisAdd
BLAST
Topological domaini61 – 11555CytoplasmicSequence analysisAdd
BLAST
Transmembranei116 – 13419HelicalSequence analysisAdd
BLAST
Topological domaini135 – 22591ExtracellularSequence analysisAdd
BLAST
Transmembranei226 – 24520HelicalSequence analysisAdd
BLAST
Topological domaini246 – 32479CytoplasmicSequence analysisAdd
BLAST
Transmembranei325 – 34420HelicalSequence analysisAdd
BLAST
Topological domaini345 – 601257ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1744487.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Regulatory protein BlaR1PRO_0000195469Add
BLAST

Structurei

Secondary structure

1
601
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi357 – 3604Combined sources
Helixi364 – 3685Combined sources
Turni369 – 3713Combined sources
Beta strandi372 – 3809Combined sources
Turni381 – 3844Combined sources
Beta strandi385 – 3895Combined sources
Helixi391 – 3955Combined sources
Helixi401 – 4044Combined sources
Helixi405 – 41410Combined sources
Turni435 – 4373Combined sources
Helixi443 – 4497Combined sources
Helixi452 – 46211Combined sources
Helixi464 – 47310Combined sources
Turni484 – 4896Combined sources
Beta strandi490 – 4934Combined sources
Helixi497 – 50812Combined sources
Turni509 – 5124Combined sources
Helixi516 – 52611Combined sources
Beta strandi527 – 5315Combined sources
Beta strandi534 – 54411Combined sources
Beta strandi546 – 5483Combined sources
Beta strandi550 – 55910Combined sources
Beta strandi564 – 57411Combined sources
Helixi578 – 59215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NRFX-ray2.50A346-601[»]
ProteinModelPortaliP12287.
SMRiP12287. Positions 352-597.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12287.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni354 – 601248Penicillin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase M56 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001460. PCN-bd_Tpept.
IPR008756. Peptidase_M56.
[Graphical view]
PfamiPF05569. Peptidase_M56. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.

Sequencei

Sequence statusi: Complete.

P12287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSFFIPFL VSQILLSLFF SIIILIKKLL RTQITVGTHY YISVISLLAL
60 70 80 90 100
IAPFIPFHFL KSHHFDWILN LGGAQSALSQ THSTDKTTEA IGQHVNWVQD
110 120 130 140 150
FSLSIEQSSS KMIDSAFFAV WILGVAVMLL ATLYSNLKIG KIKKNLQIVN
160 170 180 190 200
NKELLSLFHT CKEEIRFHQK VILSRSPLIK SPITFGVIRP YIILPKDISM
210 220 230 240 250
FSADEMKCVL LHELYHCKRK DMLINYFLCL LKIVYWFNPL VWYLSKEAKT
260 270 280 290 300
EMEISCDFAV LKTLDKKLHL KYGEVILKFT SIKQRTSSLL AASEFSSSYK
310 320 330 340 350
HIKRRIVTVV NFQTASPLLK AKSALVFTLV LGAILAGTPS VSILAMQKET
360 370 380 390 400
RFLPGTNVEY EDYSTFFDKF SASGGFVLFN SNRKKYTIYN RKESTSRFAP
410 420 430 440 450
ASTYKVFSAL LALESGIITK NDSHMTWDGT QYPYKEWNQD QDLFSAMSSS
460 470 480 490 500
TTWYFQKLDR QIGEDHLRHY LKSIHYGNED FSVPADYWLD GSLQISPLEQ
510 520 530 540 550
VNILKKFYDN EFDFKQSNIE TVKDSIRLEE SNGRVLSGKT GTSVINGELH
560 570 580 590 600
AGWFIGYVET ADNTFFFAVH IQGEKRAAGS SAAEIALSIL DKKGIYPSVS

R
Length:601
Mass (Da):68,417
Last modified:October 1, 1989 - v1
Checksum:i84677A8DA448518E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17368 Genomic DNA. Translation: AAA22273.1.
M21337 Genomic DNA. Translation: AAA22651.1.
PIRiI39790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17368 Genomic DNA. Translation: AAA22273.1.
M21337 Genomic DNA. Translation: AAA22651.1.
PIRiI39790.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NRFX-ray2.50A346-601[»]
ProteinModelPortaliP12287.
SMRiP12287. Positions 352-597.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL1744487.

Protein family/group databases

MEROPSiM56.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12287.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001460. PCN-bd_Tpept.
IPR008756. Peptidase_M56.
[Graphical view]
PfamiPF05569. Peptidase_M56. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "A second regulatory gene, blaR1, encoding a potential penicillin-binding protein required for induction of beta-lactamase in Bacillus licheniformis."
    Kobayashi T., Zhu Y.F., Nicholls N.J., Lampen J.O.
    J. Bacteriol. 169:3873-3878(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25972 / 749/C / DSM 8782 / NCIMB 11109 / IMET 10723.
  2. "Regulation of the penicillinase genes of Bacillus licheniformis: interaction of the pen repressor with its operators."
    Wittman V., Wong H.C.
    J. Bacteriol. 170:3206-3212(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.
    Strain: ATCC 25972 / 749/C / DSM 8782 / NCIMB 11109 / IMET 10723.
  3. "Identification of BlaR, the signal transducer for beta-lactamase production in Bacillus licheniformis, as a penicillin-binding protein with strong homology to the OXA-2 beta-lactamase (class D) of Salmonella typhimurium."
    Zhu Y.F., Curran I.H.A., Joris B., Ghuysen J.-M., Lampen J.O.
    J. Bacteriol. 172:1137-1141(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISCUSSION OF SEQUENCE.

Entry informationi

Entry nameiBLAR_BACLI
AccessioniPrimary (citable) accession number: P12287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 14, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.