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Protein

Molybdopterin-synthase adenylyltransferase

Gene

moeB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein MoaD.2 Publications

Catalytic activityi

ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Pathwayi: molybdopterin biosynthesis

This protein is involved in the pathway molybdopterin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway molybdopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATP; via amide nitrogen1
Binding sitei62ATP1
Binding sitei86ATP1
Metal bindingi172Zinc1
Metal bindingi175Zinc1
Metal bindingi244Zinc1
Metal bindingi247Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 73ATP5
Nucleotide bindingi130 – 131ATP2

GO - Molecular functioni

GO - Biological processi

  • cellular protein modification process Source: GO_Central
  • Mo-molybdopterin cofactor biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Molybdenum cofactor biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10154-MONOMER.
ECOL316407:JW0810-MONOMER.
MetaCyc:EG10154-MONOMER.
UniPathwayiUPA00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Molybdopterin-synthase adenylyltransferase (EC:2.7.7.80)
Alternative name(s):
MoaD protein adenylase
Molybdopterin-converting factor subunit 1 adenylase
Sulfur carrier protein MoaD adenylyltransferase
Gene namesi
Name:moeB
Synonyms:chlN
Ordered Locus Names:b0826, JW0810
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10154. moeB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14R → A or K: No effect. 1 Publication1
Mutagenesisi14R → A: No activity; when associated with A-73. 1 Publication1
Mutagenesisi44C → A: No effect. 1 Publication1
Mutagenesisi73R → A: No effect. No activity; when associated with A-14. 1 Publication1
Mutagenesisi73R → K: Substantially reduced activity. 1 Publication1
Mutagenesisi128C → A: No effect. 1 Publication1
Mutagenesisi128C → Y: No activity. 1 Publication1
Mutagenesisi130D → A: No activity. 1 Publication1
Mutagenesisi130D → E: Substantially reduced activity. 1 Publication1
Mutagenesisi142C → A: No effect. 1 Publication1
Mutagenesisi172C → A: No zinc bound and no enzyme activity. 1 Publication1
Mutagenesisi175C → A: No zinc bound and no enzyme activity. 1 Publication1
Mutagenesisi187C → A: No effect. 1 Publication1
Mutagenesisi231C → A: No effect. 1 Publication1
Mutagenesisi244C → A: No zinc bound and almost no enzyme activity. 1 Publication1
Mutagenesisi247C → A: No zinc bound and almost no enzyme activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001205751 – 249Molybdopterin-synthase adenylyltransferaseAdd BLAST249

Proteomic databases

PaxDbiP12282.
PRIDEiP12282.

Interactioni

Subunit structurei

Homodimer. Forms a stable heterotetrameric complex of 2 MoeB and 2 MoaD during adenylation of MoaD.1 Publication

Protein-protein interaction databases

BioGridi4261831. 9 interactors.
DIPiDIP-10241N.
IntActiP12282. 15 interactors.
MINTiMINT-222244.
STRINGi511145.b0826.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 11Combined sources6
Helixi13 – 16Combined sources4
Turni19 – 21Combined sources3
Helixi22 – 31Combined sources10
Beta strandi33 – 37Combined sources5
Helixi41 – 53Combined sources13
Beta strandi56 – 61Combined sources6
Helixi68 – 72Combined sources5
Helixi79 – 81Combined sources3
Helixi86 – 97Combined sources12
Beta strandi101 – 106Combined sources6
Helixi112 – 120Combined sources9
Beta strandi122 – 127Combined sources6
Helixi132 – 145Combined sources14
Beta strandi149 – 156Combined sources8
Beta strandi158 – 164Combined sources7
Helixi173 – 177Combined sources5
Helixi194 – 213Combined sources20
Beta strandi220 – 227Combined sources8
Turni228 – 231Combined sources4
Beta strandi232 – 237Combined sources6
Turni245 – 247Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JW9X-ray1.70B1-249[»]
1JWAX-ray2.90B1-249[»]
1JWBX-ray2.10B1-249[»]
ProteinModelPortaliP12282.
SMRiP12282.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12282.

Family & Domainsi

Sequence similaritiesi

Belongs to the HesA/MoeB/ThiF family.Curated

Phylogenomic databases

eggNOGiENOG4105D06. Bacteria.
COG0476. LUCA.
HOGENOMiHOG000281217.
InParanoidiP12282.
KOiK11996.
OMAiKDNNPHY.
PhylomeDBiP12282.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR012730. Mopterin_Synthase_Sase_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
TIGRFAMsiTIGR02355. moeB. 1 hit.

Sequencei

Sequence statusi: Complete.

P12282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELSDQEML RYNRQIILRG FDFDGQEALK DSRVLIVGLG GLGCAASQYL
60 70 80 90 100
ASAGVGNLTL LDFDTVSLSN LQRQTLHSDA TVGQPKVESA RDALTRINPH
110 120 130 140 150
IAITPVNALL DDAELAALIA EHDLVLDCTD NVAVRNQLNA GCFAAKVPLV
160 170 180 190 200
SGAAIRMEGQ ITVFTYQDGE PCYRCLSRLF GENALTCVEA GVMAPLIGVI
210 220 230 240
GSLQAMEAIK MLAGYGKPAS GKIVMYDAMT CQFREMKLMR NPGCEVCGQ
Length:249
Mass (Da):26,719
Last modified:October 1, 1989 - v1
Checksum:i12C77082B3F39D7D
GO

Mass spectrometryi

Molecular mass is 26563 Da from positions 1 - 249. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21151 Genomic DNA. Translation: AAA23580.1.
U00096 Genomic DNA. Translation: AAC73913.1.
AP009048 Genomic DNA. Translation: BAA35514.1.
PIRiB32352.
RefSeqiNP_415347.1. NC_000913.3.
WP_000829217.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73913; AAC73913; b0826.
BAA35514; BAA35514; BAA35514.
GeneIDi945452.
KEGGiecj:JW0810.
eco:b0826.
PATRICi32116855. VBIEscCol129921_0853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21151 Genomic DNA. Translation: AAA23580.1.
U00096 Genomic DNA. Translation: AAC73913.1.
AP009048 Genomic DNA. Translation: BAA35514.1.
PIRiB32352.
RefSeqiNP_415347.1. NC_000913.3.
WP_000829217.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JW9X-ray1.70B1-249[»]
1JWAX-ray2.90B1-249[»]
1JWBX-ray2.10B1-249[»]
ProteinModelPortaliP12282.
SMRiP12282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261831. 9 interactors.
DIPiDIP-10241N.
IntActiP12282. 15 interactors.
MINTiMINT-222244.
STRINGi511145.b0826.

Proteomic databases

PaxDbiP12282.
PRIDEiP12282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73913; AAC73913; b0826.
BAA35514; BAA35514; BAA35514.
GeneIDi945452.
KEGGiecj:JW0810.
eco:b0826.
PATRICi32116855. VBIEscCol129921_0853.

Organism-specific databases

EchoBASEiEB0152.
EcoGeneiEG10154. moeB.

Phylogenomic databases

eggNOGiENOG4105D06. Bacteria.
COG0476. LUCA.
HOGENOMiHOG000281217.
InParanoidiP12282.
KOiK11996.
OMAiKDNNPHY.
PhylomeDBiP12282.

Enzyme and pathway databases

UniPathwayiUPA00344.
BioCyciEcoCyc:EG10154-MONOMER.
ECOL316407:JW0810-MONOMER.
MetaCyc:EG10154-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP12282.
PROiP12282.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR012730. Mopterin_Synthase_Sase_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
TIGRFAMsiTIGR02355. moeB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMOEB_ECOLI
AccessioniPrimary (citable) accession number: P12282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.