Reviewed, UniProtKB/Swiss-Prot P12282 (MOEB_ECOLI)

Names and origin

Protein names

Recommended name:
    Molybdopterin biosynthesis protein moeB

Gene names

Name:	moeB
Synonyms:	chlN
Ordered Locus Names:	b0826, JW0810

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of a demolybdo cofactor (molybdopterin), necessary for molybdoenzymes. Plays a role in the activation of the small subunit of the molybdopterin converting factor (moaD).
Pathway	Cofactor biosynthesis; molybdopterin biosynthesis.
Sequence similarities	Belongs to the hesA/moeB/thiF family.

Ontologies

Keywords
Biological process	Molybdenum cofactor biosynthesis
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
dnaK	P0A6Y8	1	EBI-554435,EBI-542092
moaD	P30748	1	EBI-554435,EBI-554366

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 249

249

Molybdopterin biosynthesis protein moeB

PRO_0000120575

Secondary structure

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249

Helix Strand Turn

Details...

Helix

6 – 11

6

Helix

13 – 16

4

Turn

19 – 21

3

Helix

22 – 31

10

Beta strand

33 – 37

5

Helix

41 – 53

13

Beta strand

56 – 61

6

Helix

68 – 72

5

Helix

79 – 81

3

Helix

86 – 97

12

Beta strand

101 – 106

6

Helix

112 – 120

9

Beta strand

122 – 127

6

Helix

132 – 145

14

Beta strand

149 – 156

8

Beta strand

158 – 164

7

Helix

173 – 177

5

Helix

194 – 213

20

Beta strand

220 – 227

8

Turn

228 – 231

4

Beta strand

232 – 237

6

Turn

245 – 247

3

Sequences

	Sequence		Length	Mass (Da)
	P12282-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 12C77082B3F39D7D	FASTA	249	26,719
	10 20 30 40 50 60 MAELSDQEML RYNRQIILRG FDFDGQEALK DSRVLIVGLG GLGCAASQYL ASAGVGNLTL 70 80 90 100 110 120 LDFDTVSLSN LQRQTLHSDA TVGQPKVESA RDALTRINPH IAITPVNALL DDAELAALIA 130 140 150 160 170 180 EHDLVLDCTD NVAVRNQLNA GCFAAKVPLV SGAAIRMEGQ ITVFTYQDGE PCYRCLSRLF 190 200 210 220 230 240 GENALTCVEA GVMAPLIGVI GSLQAMEAIK MLAGYGKPAS GKIVMYDAMT CQFREMKLMR NPGCEVCGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the Escherichia coli chlEN operon involved in molybdopterin biosynthesis." Nohno T., Kasai Y., Saito T. J. Bacteriol. 170:4097-4102(1988) [PubMed: 3045084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M21151 Genomic DNA. Translation: AAA23580.1.
U00096 Genomic DNA. Translation: AAC73913.1.
AP009048 Genomic DNA. Translation: BAA35514.1.

PIR

B32352.

RefSeq

AP_001457.1.
NP_415347.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JW9	X-ray	1.70	B	1-249	[»]
1JWA	X-ray	2.90	B	1-249	[»]
1JWB	X-ray	2.10	B	1-249	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-10241N.

IntAct

P12282. 15 interactions.

STRING

P12282.

Genome annotation databases

GeneID

945452.

GenomeReviews

Gene locus JW0810 in contig AP009048_GR.
Gene locus b0826 in contig U00096_GR.

KEGG

ecj:JW0810.
eco:b0826.

Organism-specific databases

EchoBASE

EB0152.

EcoGene

EG10154. moeB.

CMR

Search...

Phylogenomic databases

eggNOG

COG0476.

HOGENOM

HBG535305.

OMA

SRLFSEN.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10154-MONOMER.
ECOL168927:B0826-MONOMER.
MetaCyc:EG10154-MONOMER.

Gene expression databases

Genevestigator

P12282.

Family and domain databases

InterPro

IPR007901. MoeZ_MoeB.
IPR009036. Molybdenum_cofac_synth_MoeB.
IPR012730. Mopterin_Synthase_Sase_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD_bd.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF05237. MoeZ_MoeB. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02355. moeB. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MOEB_ECOLI

Accession

Primary (citable) accession number: P12282

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	February 9, 2010
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)