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Protein

Creatine kinase B-type

Gene

CKB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72Substrate; via amide nitrogen1
Binding sitei130ATP1
Binding sitei132ATP1
Binding sitei191ATP1
Binding sitei232Substrate1
Binding sitei236ATP1
Binding sitei285Substrate1
Binding sitei292ATP1
Binding sitei320ATP1
Binding sitei335ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATP5
Nucleotide bindingi320 – 325ATP6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • creatine kinase activity Source: AgBase
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS09344-MONOMER.
ZFISH:HS09344-MONOMER.
BRENDAi2.7.3.2. 2681.
ReactomeiR-HSA-71288. Creatine metabolism.
SABIO-RKP12277.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-type (EC:2.7.3.2)
Alternative name(s):
B-CK
Creatine kinase B chain
Gene namesi
Name:CKB
Synonyms:CKBB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:1991. CKB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi283C → S or Y: Complete loss of activity. 1 Publication1
Mutagenesisi292R → H, L or Q: Complete loss of activity. 1 Publication1
Mutagenesisi292R → K: 42% of wild-type activity. 1 Publication1
Mutagenesisi340D → E: No change in activity. 1 Publication1

Organism-specific databases

DisGeNETi1152.
OpenTargetsiENSG00000166165.
PharmGKBiPA26528.

Chemistry databases

ChEMBLiCHEMBL6049.
DrugBankiDB00148. Creatine.

Polymorphism and mutation databases

BioMutaiCKB.
DMDMi125294.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00002119662 – 381Creatine kinase B-typeAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineCombined sources1
Modified residuei35PhosphothreonineCombined sources1
Modified residuei125PhosphotyrosineBy similarity1
Modified residuei199PhosphoserineCombined sources1
Modified residuei269Nitrated tyrosineBy similarity1
Modified residuei309PhosphoserineBy similarity1
Modified residuei322PhosphothreonineBy similarity1

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

EPDiP12277.
PaxDbiP12277.
PeptideAtlasiP12277.
PRIDEiP12277.
TopDownProteomicsiP12277.

2D gel databases

REPRODUCTION-2DPAGEIPI00022977.
P12277.
UCD-2DPAGEP12277.

PTM databases

iPTMnetiP12277.
PhosphoSitePlusiP12277.

Expressioni

Gene expression databases

BgeeiENSG00000166165.
CleanExiHS_CKB.
ExpressionAtlasiP12277. baseline and differential.
GenevisibleiP12277. HS.

Organism-specific databases

HPAiCAB047313.
HPA001254.

Interactioni

Subunit structurei

Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Q034633EBI-357706,EBI-9081620From a different organism.
ASB9Q96DX58EBI-357706,EBI-745641

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi107572. 78 interactors.
IntActiP12277. 40 interactors.
MINTiMINT-1484821.
STRINGi9606.ENSP00000299198.

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 11Combined sources5
Helixi16 – 19Combined sources4
Helixi29 – 33Combined sources5
Helixi36 – 42Combined sources7
Helixi53 – 62Combined sources10
Beta strandi67 – 69Combined sources3
Helixi81 – 84Combined sources4
Helixi86 – 96Combined sources11
Helixi112 – 114Combined sources3
Turni123 – 125Combined sources3
Beta strandi126 – 135Combined sources10
Turni143 – 145Combined sources3
Helixi148 – 162Combined sources15
Helixi167 – 169Combined sources3
Beta strandi171 – 176Combined sources6
Helixi181 – 189Combined sources9
Helixi200 – 203Combined sources4
Turni204 – 214Combined sources11
Beta strandi216 – 220Combined sources5
Beta strandi223 – 244Combined sources22
Helixi246 – 266Combined sources21
Turni275 – 277Combined sources3
Helixi284 – 286Combined sources3
Beta strandi292 – 298Combined sources7
Helixi300 – 303Combined sources4
Helixi308 – 314Combined sources7
Beta strandi317 – 321Combined sources5
Helixi325 – 330Combined sources6
Beta strandi333 – 338Combined sources6
Beta strandi342 – 344Combined sources3
Helixi346 – 368Combined sources23
Helixi374 – 376Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B6RX-ray2.00A/B1-381[»]
3DRBX-ray2.00A/B1-381[»]
3DREX-ray2.20A/B1-381[»]
ProteinModelPortaliP12277.
SMRiP12277.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12277.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP12277.
KOiK00933.
OMAiRGHEFMW.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP12277.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG
60 70 80 90 100
FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY
110 120 130 140 150
KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Length:381
Mass (Da):42,644
Last modified:October 1, 1989 - v1
Checksum:i637AA67A86AE3059
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41 – 42EL → DV in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti78D → G in AAA76850 (PubMed:2883200).Curated1
Sequence conflicti78D → G in AAA52024 (PubMed:2828370).Curated1
Sequence conflicti98 – 99GG → RR in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti105 – 106EH → DD in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti130R → G in AAA76850 (PubMed:2883200).Curated1
Sequence conflicti132R → A in AAA76851 (PubMed:3034271).Curated1
Sequence conflicti144P → Q in CAG47064 (Ref. 6) Curated1
Sequence conflicti203L → S in AAC31758 (PubMed:3692484).Curated1
Sequence conflicti215 – 216RG → AR in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti296H → D in AAA76851 (PubMed:3034271).Curated1
Sequence conflicti358K → R in BAG35211 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025838177K → R.1 PublicationCorresponds to variant rs36002620dbSNPEnsembl.1
Natural variantiVAR_025839309S → L.1 PublicationCorresponds to variant rs35156510dbSNPEnsembl.1
Natural variantiVAR_049674360L → F.Corresponds to variant rs12505dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16451 mRNA. Translation: AAA76851.1.
M21243
, M21237, M21238, M21239, M21240, M21241, M21242 Genomic DNA. Translation: AAC31758.1.
L47647 mRNA. Translation: AAA76852.1.
M16364 mRNA. Translation: AAA76850.1.
X15334 Genomic DNA. Translation: CAA33389.1.
AK290101 mRNA. Translation: BAF82790.1.
AK312282 mRNA. Translation: BAG35211.1.
CR542268 mRNA. Translation: CAG47064.1.
DQ333313 Genomic DNA. Translation: ABC67465.1.
CH471061 Genomic DNA. Translation: EAW81822.1.
BC001190 mRNA. Translation: AAH01190.1.
BC004914 mRNA. Translation: AAH04914.1.
BC008323 mRNA. Translation: AAH08323.1.
BC010002 mRNA. Translation: AAH10002.1.
BC019259 mRNA. Translation: AAH19259.1.
BC019281 mRNA. Translation: AAH19281.1.
M22356, M22355 Genomic DNA. Translation: AAA52024.1.
CCDSiCCDS9981.1.
PIRiS15935. KIHUCB.
RefSeqiNP_001814.2. NM_001823.4.
UniGeneiHs.173724.

Genome annotation databases

EnsembliENST00000348956; ENSP00000299198; ENSG00000166165.
GeneIDi1152.
KEGGihsa:1152.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs
Wikipedia

Creatine kinase entry

Wikipedia

CKB entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16451 mRNA. Translation: AAA76851.1.
M21243
, M21237, M21238, M21239, M21240, M21241, M21242 Genomic DNA. Translation: AAC31758.1.
L47647 mRNA. Translation: AAA76852.1.
M16364 mRNA. Translation: AAA76850.1.
X15334 Genomic DNA. Translation: CAA33389.1.
AK290101 mRNA. Translation: BAF82790.1.
AK312282 mRNA. Translation: BAG35211.1.
CR542268 mRNA. Translation: CAG47064.1.
DQ333313 Genomic DNA. Translation: ABC67465.1.
CH471061 Genomic DNA. Translation: EAW81822.1.
BC001190 mRNA. Translation: AAH01190.1.
BC004914 mRNA. Translation: AAH04914.1.
BC008323 mRNA. Translation: AAH08323.1.
BC010002 mRNA. Translation: AAH10002.1.
BC019259 mRNA. Translation: AAH19259.1.
BC019281 mRNA. Translation: AAH19281.1.
M22356, M22355 Genomic DNA. Translation: AAA52024.1.
CCDSiCCDS9981.1.
PIRiS15935. KIHUCB.
RefSeqiNP_001814.2. NM_001823.4.
UniGeneiHs.173724.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B6RX-ray2.00A/B1-381[»]
3DRBX-ray2.00A/B1-381[»]
3DREX-ray2.20A/B1-381[»]
ProteinModelPortaliP12277.
SMRiP12277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107572. 78 interactors.
IntActiP12277. 40 interactors.
MINTiMINT-1484821.
STRINGi9606.ENSP00000299198.

Chemistry databases

ChEMBLiCHEMBL6049.
DrugBankiDB00148. Creatine.

PTM databases

iPTMnetiP12277.
PhosphoSitePlusiP12277.

Polymorphism and mutation databases

BioMutaiCKB.
DMDMi125294.

2D gel databases

REPRODUCTION-2DPAGEIPI00022977.
P12277.
UCD-2DPAGEP12277.

Proteomic databases

EPDiP12277.
PaxDbiP12277.
PeptideAtlasiP12277.
PRIDEiP12277.
TopDownProteomicsiP12277.

Protocols and materials databases

DNASUi1152.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000348956; ENSP00000299198; ENSG00000166165.
GeneIDi1152.
KEGGihsa:1152.

Organism-specific databases

CTDi1152.
DisGeNETi1152.
GeneCardsiCKB.
HGNCiHGNC:1991. CKB.
HPAiCAB047313.
HPA001254.
MIMi123280. gene.
neXtProtiNX_P12277.
OpenTargetsiENSG00000166165.
PharmGKBiPA26528.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP12277.
KOiK00933.
OMAiRGHEFMW.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP12277.
TreeFamiTF314214.

Enzyme and pathway databases

BioCyciMetaCyc:HS09344-MONOMER.
ZFISH:HS09344-MONOMER.
BRENDAi2.7.3.2. 2681.
ReactomeiR-HSA-71288. Creatine metabolism.
SABIO-RKP12277.

Miscellaneous databases

ChiTaRSiCKB. human.
EvolutionaryTraceiP12277.
GeneWikiiCKB_(gene).
GenomeRNAii1152.
PROiP12277.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000166165.
CleanExiHS_CKB.
ExpressionAtlasiP12277. baseline and differential.
GenevisibleiP12277. HS.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCRB_HUMAN
AccessioniPrimary (citable) accession number: P12277
Secondary accession number(s): A8K236
, B2R5R4, Q2LE07, Q6FG40, Q9UC66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 30, 2016
This is version 179 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.