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Protein

Creatine kinase B-type

Gene

CKB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72Substrate; via amide nitrogen1 Publication1
Binding sitei130ATP1 Publication1
Binding sitei132ATP1 Publication1
Binding sitei191ATP1 Publication1
Binding sitei232Substrate1 Publication1
Binding sitei236ATP1 Publication1
Binding sitei285Substrate1 Publication1
Binding sitei292ATP1 Publication1
Binding sitei320ATP1 Publication1
Binding sitei335ATP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 132ATP1 Publication5
Nucleotide bindingi320 – 325ATP1 Publication6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • creatine kinase activity Source: AgBase
  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS09344-MONOMER
BRENDAi2.7.3.2 2681
ReactomeiR-HSA-71288 Creatine metabolism
SABIO-RKiP12277

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase B-typeCurated (EC:2.7.3.2)
Alternative name(s):
Brain creatine kinase1 Publication
Short name:
B-CK
Creatine kinase B chain1 Publication
Creatine phosphokinase B-type
Short name:
CPK-B
Gene namesi
Name:CKB
Synonyms:CKBB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000166165.12
HGNCiHGNC:1991 CKB
MIMi123280 gene
neXtProtiNX_P12277

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi283C → S or Y: Complete loss of activity. 1 Publication1
Mutagenesisi292R → H, L or Q: Complete loss of activity. 1 Publication1
Mutagenesisi292R → K: 42% of wild-type activity. 1 Publication1
Mutagenesisi340D → E: No change in activity. 1 Publication1

Organism-specific databases

DisGeNETi1152
OpenTargetsiENSG00000166165
PharmGKBiPA26528

Chemistry databases

ChEMBLiCHEMBL6049
DrugBankiDB00148 Creatine

Polymorphism and mutation databases

BioMutaiCKB
DMDMi125294

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00002119662 – 381Creatine kinase B-typeAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4PhosphoserineCombined sources1
Modified residuei35PhosphothreonineCombined sources1
Modified residuei125PhosphotyrosineBy similarity1
Modified residuei199PhosphoserineCombined sources1
Modified residuei269Nitrated tyrosineBy similarity1
Modified residuei309PhosphoserineBy similarity1
Modified residuei322PhosphothreonineBy similarity1

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

EPDiP12277
PaxDbiP12277
PeptideAtlasiP12277
PRIDEiP12277
ProteomicsDBi52846
TopDownProteomicsiP12277

2D gel databases

REPRODUCTION-2DPAGEiIPI00022977
P12277
UCD-2DPAGEiP12277

PTM databases

CarbonylDBiP12277
iPTMnetiP12277
PhosphoSitePlusiP12277

Expressioni

Gene expression databases

BgeeiENSG00000166165
CleanExiHS_CKB
ExpressionAtlasiP12277 baseline and differential
GenevisibleiP12277 HS

Organism-specific databases

HPAiCAB047313
HPA001254

Interactioni

Subunit structurei

Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: ParkinsonsUK-UCL

Protein-protein interaction databases

BioGridi107572, 84 interactors
CORUMiP12277
DIPiDIP-52968N
IntActiP12277, 45 interactors
MINTiP12277
STRINGi9606.ENSP00000299198

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 11Combined sources5
Helixi16 – 19Combined sources4
Helixi29 – 33Combined sources5
Helixi36 – 42Combined sources7
Helixi53 – 62Combined sources10
Beta strandi67 – 69Combined sources3
Helixi81 – 84Combined sources4
Helixi86 – 96Combined sources11
Helixi112 – 114Combined sources3
Turni123 – 125Combined sources3
Beta strandi126 – 135Combined sources10
Turni143 – 145Combined sources3
Helixi148 – 162Combined sources15
Helixi167 – 169Combined sources3
Beta strandi171 – 176Combined sources6
Helixi181 – 189Combined sources9
Helixi200 – 203Combined sources4
Turni204 – 214Combined sources11
Beta strandi216 – 220Combined sources5
Beta strandi223 – 244Combined sources22
Helixi246 – 266Combined sources21
Turni275 – 277Combined sources3
Helixi284 – 286Combined sources3
Beta strandi292 – 298Combined sources7
Helixi300 – 303Combined sources4
Helixi308 – 314Combined sources7
Beta strandi317 – 321Combined sources5
Helixi325 – 330Combined sources6
Beta strandi333 – 338Combined sources6
Beta strandi342 – 344Combined sources3
Helixi346 – 368Combined sources23
Helixi374 – 376Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B6RX-ray2.00A/B1-381[»]
3DRBX-ray2.00A/B1-381[»]
3DREX-ray2.20A/B1-381[»]
ProteinModelPortaliP12277
SMRiP12277
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12277

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 98Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST88
Domaini125 – 367Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3581 Eukaryota
COG3869 LUCA
GeneTreeiENSGT00550000074561
HOGENOMiHOG000232165
HOVERGENiHBG001339
InParanoidiP12277
KOiK00933
OMAiRGHEFMW
OrthoDBiEOG091G0HZ0
PhylomeDBiP12277
TreeFamiTF314214

Family and domain databases

Gene3Di1.10.135.10, 1 hit
InterProiView protein in InterPro
IPR000749 ATP-guanido_PTrfase
IPR022415 ATP-guanido_PTrfase_AS
IPR022414 ATP-guanido_PTrfase_cat
IPR022413 ATP-guanido_PTrfase_N
IPR036802 ATP-guanido_PTrfase_N_sf
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11547 PTHR11547, 1 hit
PfamiView protein in Pfam
PF00217 ATP-gua_Ptrans, 1 hit
PF02807 ATP-gua_PtransN, 1 hit
SUPFAMiSSF48034 SSF48034, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00112 PHOSPHAGEN_KINASE, 1 hit
PS51510 PHOSPHAGEN_KINASE_C, 1 hit
PS51509 PHOSPHAGEN_KINASE_N, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG
60 70 80 90 100
FTLDDVIQTG VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY
110 120 130 140 150
KPSDEHKTDL NPDNLQGGDD LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE
160 170 180 190 200
RRAIEKLAVE ALSSLDGDLA GRYYALKSMT EAEQQQLIDD HFLFDKPVSP
210 220 230 240 250
LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM QKGGNMKEVF
260 270 280 290 300
TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
310 320 330 340 350
NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV
360 370 380
QMVVDGVKLL IEMEQRLEQG QAIDDLMPAQ K
Length:381
Mass (Da):42,644
Last modified:October 1, 1989 - v1
Checksum:i637AA67A86AE3059
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41 – 42EL → DV in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti78D → G in AAA76850 (PubMed:2883200).Curated1
Sequence conflicti78D → G in AAA52024 (PubMed:2828370).Curated1
Sequence conflicti98 – 99GG → RR in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti105 – 106EH → DD in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti130R → G in AAA76850 (PubMed:2883200).Curated1
Sequence conflicti132R → A in AAA76851 (PubMed:3034271).Curated1
Sequence conflicti144P → Q in CAG47064 (Ref. 6) Curated1
Sequence conflicti203L → S in AAC31758 (PubMed:3692484).Curated1
Sequence conflicti215 – 216RG → AR in AAA76851 (PubMed:3034271).Curated2
Sequence conflicti296H → D in AAA76851 (PubMed:3034271).Curated1
Sequence conflicti358K → R in BAG35211 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_025838177K → R1 PublicationCorresponds to variant dbSNP:rs36002620Ensembl.1
Natural variantiVAR_025839309S → L1 PublicationCorresponds to variant dbSNP:rs35156510Ensembl.1
Natural variantiVAR_049674360L → F. Corresponds to variant dbSNP:rs12505Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16451 mRNA Translation: AAA76851.1
M21243
, M21237, M21238, M21239, M21240, M21241, M21242 Genomic DNA Translation: AAC31758.1
L47647 mRNA Translation: AAA76852.1
M16364 mRNA Translation: AAA76850.1
X15334 Genomic DNA Translation: CAA33389.1
AK290101 mRNA Translation: BAF82790.1
AK312282 mRNA Translation: BAG35211.1
CR542268 mRNA Translation: CAG47064.1
DQ333313 Genomic DNA Translation: ABC67465.1
CH471061 Genomic DNA Translation: EAW81822.1
BC001190 mRNA Translation: AAH01190.1
BC004914 mRNA Translation: AAH04914.1
BC008323 mRNA Translation: AAH08323.1
BC010002 mRNA Translation: AAH10002.1
BC019259 mRNA Translation: AAH19259.1
BC019281 mRNA Translation: AAH19281.1
M22356, M22355 Genomic DNA Translation: AAA52024.1
CCDSiCCDS9981.1
PIRiS15935 KIHUCB
RefSeqiNP_001814.2, NM_001823.4
UniGeneiHs.173724

Genome annotation databases

EnsembliENST00000348956; ENSP00000299198; ENSG00000166165
GeneIDi1152
KEGGihsa:1152

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKCRB_HUMAN
AccessioniPrimary (citable) accession number: P12277
Secondary accession number(s): A8K236
, B2R5R4, Q2LE07, Q6FG40, Q9UC66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 20, 2018
This is version 191 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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