P12273 (PIP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prolactin-inducible protein

Alternative name(s):
Gross cystic disease fluid protein 15
Short name=GCDFP-15
Prolactin-induced protein
Secretory actin-binding protein
Short name=SABP
gp17

Gene names

Name:	PIP
Synonyms:	GCDFP15, GPIP4

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Monomer. Interacts with AZGP1.
Subcellular location	Secreted.
Tissue specificity	Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
Induction	By prolactin and androgen; inhibited by estrogen.
Sequence similarities	Belongs to the PIP family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Actin-binding
PTM	Disulfide bond Glycoprotein Pyrrolidone carboxylic acid
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	extracellular region Non-traceable author statement. Source: UniProtKB
Molecular function	actin binding Non-traceable author statement Ref.8. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
WBP5	Q9UHQ7	1	EBI-1049746,EBI-1051372

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

28

Ref.8

Chain

29 – 146

118

Prolactin-inducible protein Ref.8

PRO_0000024288

Amino acid modifications

Modified residue

29

1

Pyrrolidone carboxylic acid Ref.8

Glycosylation

105

1

N-linked (GlcNAc...) Ref.8 Ref.10 Ref.11 Ref.12

Disulfide bond

65 ↔ 91

Ref.8 Ref.12

Disulfide bond

89 ↔ 123

Ref.8 Ref.12

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

146

Helix Strand Turn

Details...

Beta strand

36 – 38

3

Beta strand

44 – 46

3

Beta strand

52 – 60

9

Beta strand

66 – 76

11

Helix

80 – 83

4

Beta strand

86 – 90

5

Beta strand

92 – 94

3

Beta strand

96 – 102

7

Beta strand

108 – 116

9

Beta strand

120 – 123

4

Helix

124 – 126

3

Beta strand

129 – 133

5

Beta strand

140 – 145

6

Sequences

	Sequence		Length	Mass (Da)
	P12273 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 93F3DA201133F03C	FASTA	146	16,572
	10 20 30 40 50 60 MRLLQLLFRA SPATLLLVLC LQLGANKAQD NTRKIIIKNF DIPKSVRPND EVTAVLAVQT 70 80 90 100 110 120 ELKECMVVKT YLISSIPLQG AFNYKYTACL CDDNPKTFYW DFYTNRTVQI AAVVDVIREL 130 140 GICPDDAAVI PIKNNRFYTI EILKVE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and sequencing of a cDNA clone for a prolactin-inducible protein (PIP). Regulation of PIP gene expression in the human breast cancer cell line, T-47D." Murphy L.C., Tsuyuki D., Myal Y., Shiu R.P.C. J. Biol. Chem. 262:15236-15241(1987) [PubMed: 3667631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The prolactin-inducible protein (PIP/GCDFP-15) gene: cloning, structure and regulation." Myal Y., Iwasiow B., Tsuyuki D., Wong P., Shiu R.P.C. Mol. Cell. Endocrinol. 80:165-175(1991) [PubMed: 1955075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Isolation from a human seminal vesicle library of the cDNA for gp17, a CD4 binding factor." Autiero M., Bouchier C., Basmaciogullari S., Zaborski P., el Marhomy S., Martin M., Guardiola J., Piatier-Tonneau D. Immunogenetics 46:345-348(1997) [PubMed: 9218538] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seminal vesicle.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Trachea.
[5]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed: 12690205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[8]	"Primary structure of a new actin-binding protein from human seminal plasma." Schaller J., Akiyama K., Kimura H., Hess D., Affolter M., Rickli E.E. Eur. J. Biochem. 196:743-750(1991) [PubMed: 2013294] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-146, DISULFIDE BONDS, GLYCOSYLATION AT ASN-105.
[9]	"Origin and evolution of gene for prolactin-induced protein." Kitano T., Tian W., Umetsu K., Yuasa I., Yamazaki K., Saitou N., Osawa M. Gene 383:64-70(2006) [PubMed: 16949771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-146.
[10]	"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry." Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A. J. Proteome Res. 5:1493-1503(2006) [PubMed: 16740002] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-105, MASS SPECTROMETRY. Tissue: Saliva.
[11]	"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry." Picariello G., Ferranti P., Mamone G., Roepstorff P., Addeo F. Proteomics 8:3833-3847(2008) [PubMed: 18780401] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-105, MASS SPECTROMETRY. Tissue: Milk.
[12]	"Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma." Hassan M.I., Bilgrami S., Kumar V., Singh N., Yadav S., Kaur P., Singh T.P. J. Mol. Biol. 384:663-672(2008) [PubMed: 18930737] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 29-146 IN COMPLEX WITH AZGP1, DISULFIDE BONDS, GLYCOSYLATION AT ASN-105.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J03460 mRNA. Translation: AAA60091.1.
X51501, X51502, X51504 Genomic DNA. Translation: CAA35870.1.
Y10179 mRNA. Translation: CAA71252.1.
AK312227 mRNA. Translation: BAG35160.1.
CH236959 Genomic DNA. Translation: EAL23781.1.
CH471198 Genomic DNA. Translation: EAW51887.1.
BC010950 mRNA. Translation: AAH10950.1.
BC010951 mRNA. Translation: AAH10951.1.
AB267097 Genomic DNA. Translation: BAF35627.1.
AB267098 Genomic DNA. Translation: BAF35628.1.
AB267099 Genomic DNA. Translation: BAF35629.1.
AB267100 Genomic DNA. Translation: BAF35630.1.
AB267101 Genomic DNA. Translation: BAF35631.1.
AB267102 Genomic DNA. Translation: BAF35632.1.
AB267103 Genomic DNA. Translation: BAF35633.1.
AB267104 Genomic DNA. Translation: BAF35634.1.
AB267105 Genomic DNA. Translation: BAF35635.1.
AB267106 Genomic DNA. Translation: BAF35636.1.
AB267107 Genomic DNA. Translation: BAF35637.1.
AB267108 Genomic DNA. Translation: BAF35638.1.
AB267109 Genomic DNA. Translation: BAF35639.1.
AB267110 Genomic DNA. Translation: BAF35640.1.
AB267111 Genomic DNA. Translation: BAF35641.1.
AB267112 Genomic DNA. Translation: BAF35642.1.
AB267113 Genomic DNA. Translation: BAF35643.1.
AB267114 Genomic DNA. Translation: BAF35644.1.
AB267115 Genomic DNA. Translation: BAF35645.1.
AB267116 Genomic DNA. Translation: BAF35646.1.
AB267117 Genomic DNA. Translation: BAF35647.1.
AB267118 Genomic DNA. Translation: BAF35648.1.
AB267119 Genomic DNA. Translation: BAF35649.1.
AB267120 Genomic DNA. Translation: BAF35650.1.
AB267121 Genomic DNA. Translation: BAF35651.1.
AB267122 Genomic DNA. Translation: BAF35652.1.
AB267123 Genomic DNA. Translation: BAF35653.1.
AB267124 Genomic DNA. Translation: BAF35654.1.
AB267125 Genomic DNA. Translation: BAF35655.1.
AB267126 Genomic DNA. Translation: BAF35656.1.
AB267127 Genomic DNA. Translation: BAF35657.1.
AB267128 Genomic DNA. Translation: BAF35658.1.
AB267129 Genomic DNA. Translation: BAF35659.1.
AB267130 Genomic DNA. Translation: BAF35660.1.
AB267131 Genomic DNA. Translation: BAF35661.1.
AB267132 Genomic DNA. Translation: BAF35662.1.
AB267133 Genomic DNA. Translation: BAF35663.1.
AB267134 Genomic DNA. Translation: BAF35664.1.
AB267135 Genomic DNA. Translation: BAF35665.1.
AB267136 Genomic DNA. Translation: BAF35666.1.
AB267137 Genomic DNA. Translation: BAF35667.1.
AB267138 Genomic DNA. Translation: BAF35668.1.
AB267139 Genomic DNA. Translation: BAF35669.1.
AB267140 Genomic DNA. Translation: BAF35670.1.
AB267141 Genomic DNA. Translation: BAF35671.1.
AB267142 Genomic DNA. Translation: BAF35672.1.
AB267143 Genomic DNA. Translation: BAF35673.1.
AB267144 Genomic DNA. Translation: BAF35674.1.
AB267145 Genomic DNA. Translation: BAF35675.1.
AB267146 Genomic DNA. Translation: BAF35676.1.
AB267147 Genomic DNA. Translation: BAF35677.1.
AB267148 Genomic DNA. Translation: BAF35678.1.
AB267149 Genomic DNA. Translation: BAF35679.1.
AB267150 Genomic DNA. Translation: BAF35680.1.
AB267151 Genomic DNA. Translation: BAF35681.1.
AB267152 Genomic DNA. Translation: BAF35682.1.
AB267153 Genomic DNA. Translation: BAF35683.1.
AB267154 Genomic DNA. Translation: BAF35684.1.
AB267155 Genomic DNA. Translation: BAF35685.1.
AB267156 Genomic DNA. Translation: BAF35686.1.
AB267157 Genomic DNA. Translation: BAF35687.1.
AB267158 Genomic DNA. Translation: BAF35688.1.
AB267159 Genomic DNA. Translation: BAF35689.1.
AB267160 Genomic DNA. Translation: BAF35690.1.
AB267161 Genomic DNA. Translation: BAF35691.1.
AB267162 Genomic DNA. Translation: BAF35692.1.
AB267163 Genomic DNA. Translation: BAF35693.1.
AB267164 Genomic DNA. Translation: BAF35694.1.
AB267165 Genomic DNA. Translation: BAF35695.1.
AB267166 Genomic DNA. Translation: BAF35696.1.
AB267167 Genomic DNA. Translation: BAF35697.1.
AB267168 Genomic DNA. Translation: BAF35698.1.
AB267169 Genomic DNA. Translation: BAF35699.1.
AB267170 Genomic DNA. Translation: BAF35700.1.
AB267171 Genomic DNA. Translation: BAF35701.1.
AB267172 Genomic DNA. Translation: BAF35702.1.
AB267173 Genomic DNA. Translation: BAF35703.1.
AB267174 Genomic DNA. Translation: BAF35704.1.
AB267175 Genomic DNA. Translation: BAF35705.1.
AB267176 Genomic DNA. Translation: BAF35706.1.
AB267177 Genomic DNA. Translation: BAF35707.1.
AB267178 Genomic DNA. Translation: BAF35708.1.
AB267179 Genomic DNA. Translation: BAF35709.1.
AB267180 Genomic DNA. Translation: BAF35710.1.
AB267181 Genomic DNA. Translation: BAF35711.1.
AB267182 Genomic DNA. Translation: BAF35712.1.
AB267183 Genomic DNA. Translation: BAF35713.1.
AB267184 Genomic DNA. Translation: BAF35714.1.
AB267185 Genomic DNA. Translation: BAF35715.1.
AB267186 Genomic DNA. Translation: BAF35716.1.
AB267187 Genomic DNA. Translation: BAF35717.1.
AB267188 Genomic DNA. Translation: BAF35718.1.
AB267189 Genomic DNA. Translation: BAF35719.1.
AB267190 Genomic DNA. Translation: BAF35720.1.
AB267191 Genomic DNA. Translation: BAF35721.1.
AB267192 Genomic DNA. Translation: BAF35722.1.
AB267193 Genomic DNA. Translation: BAF35723.1.
AB267194 Genomic DNA. Translation: BAF35724.1.
AB267195 Genomic DNA. Translation: BAF35725.1.
AB267196 Genomic DNA. Translation: BAF35726.1.
AB267197 Genomic DNA. Translation: BAF35727.1.
AB267198 Genomic DNA. Translation: BAF35728.1.
AB267199 Genomic DNA. Translation: BAF35729.1.
AB267200 Genomic DNA. Translation: BAF35730.1.
AB267201 Genomic DNA. Translation: BAF35731.1.
AB267202 Genomic DNA. Translation: BAF35732.1.
AB267203 Genomic DNA. Translation: BAF35733.1.
AB267204 Genomic DNA. Translation: BAF35734.1.
AB267205 Genomic DNA. Translation: BAF35735.1.
AB267206 Genomic DNA. Translation: BAF35736.1.
AB267207 Genomic DNA. Translation: BAF35737.1.
AB267208 Genomic DNA. Translation: BAF35738.1.
AB267209 Genomic DNA. Translation: BAF35739.1.
AB267210 Genomic DNA. Translation: BAF35740.1.
AB267211 Genomic DNA. Translation: BAF35741.1.
AB267212 Genomic DNA. Translation: BAF35742.1.
AB267213 Genomic DNA. Translation: BAF35743.1.
AB267214 Genomic DNA. Translation: BAF35744.1.
AB267215 Genomic DNA. Translation: BAF35745.1.
AB267216 Genomic DNA. Translation: BAF35746.1.

IPI

IPI00022974.

PIR

SQHUAC. I37432.

RefSeq

NP_002643.1. NM_002652.2.

UniGene

Hs.99949.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ES6	X-ray	3.23	B	29-146	[»]

ProteinModelPortal

P12273.

SMR

P12273. Positions 29-146.

ModBase

Search...

Protein-protein interaction databases

IntAct

P12273. 9 interactions.

STRING

P12273.

PTM databases

PhosphoSite

P12273.

Polymorphism databases

DMDM

134170.

Proteomic databases

PeptideAtlas

P12273.

PRIDE

P12273.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000291009; ENSP00000291009; ENSG00000159763.

GeneID

5304.

KEGG

hsa:5304.

UCSC

uc003wcf.1. human.

Organism-specific databases

CTD

5304.

GeneCards

GC07P142829.

H-InvDB

HIX0007165.

HGNC

HGNC:8993. PIP.

HPA

CAB002661.
HPA009177.

MIM

176720. gene.

neXtProt

NX_P12273.

PharmGKB

PA33326.

GenAtlas

Search...

Phylogenomic databases

GeneTree

ENSGT00390000002099.

HOGENOM

HBG126687.

HOVERGEN

HBG065978.

InParanoid

P12273.

OMA

TELKECM.

OrthoDB

EOG4GQQ69.

PhylomeDB

P12273.

Gene expression databases

ArrayExpress

P12273.

Bgee

P12273.

CleanEx

HS_PIP.

Genevestigator

P12273.

GermOnline

ENSG00000159763. Homo sapiens.

Family and domain databases

InterPro

IPR007990. SV_autoAg.
[Graphical view]

PANTHER

PTHR15096. SV_autoAg. 1 hit.

Pfam

PF05326. SVA. 1 hit.
[Graphical view]

PIRSF

PIRSF002572. PIP-GCDFP-15. 1 hit.

ProDom

PD021604. SV_autoAg. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

NextBio

20502.

SOURCE

Search...

Entry information

Entry name

PIP_HUMAN

Accession

Primary (citable) accession number: P12273
Secondary accession number(s): A0A963 , A0A9C3, A0A9F3, A4D2I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	January 25, 2012
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.