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Protein

Beta-glucuronidase

Gene

Gusb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the degradation of dermatan and keratan sulfates.

Catalytic activityi

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

Enzyme regulationi

Inhibited by L-aspartic acid.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei447Proton donorBy similarity1

GO - Molecular functioni

  • beta-glucuronidase activity Source: MGI
  • hydrolase activity Source: MGI
  • protein domain specific binding Source: MGI
  • receptor binding Source: MGI

GO - Biological processi

  • carbohydrate metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-2024096. HS-GAG degradation.
R-MMU-2160916. Hyaluronan uptake and degradation.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucuronidase (EC:3.2.1.31)
Gene namesi
Name:Gusb
Synonyms:Gus, Gus-s
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:95872. Gusb.

Subcellular locationi

  • Lysosome 1 Publication
  • Endoplasmic reticulum 1 Publication

  • Note: A small proportion is found in the endoplasmic reticulum.

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • lysosome Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000001216223 – 648Beta-glucuronidaseAdd BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Glycosylationi416N-linked (GlcNAc...)Sequence analysis1
Glycosylationi591N-linked (GlcNAc...)Sequence analysis1
Glycosylationi627N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP12265.
PaxDbiP12265.
PeptideAtlasiP12265.
PRIDEiP12265.

PTM databases

PhosphoSitePlusiP12265.
SwissPalmiP12265.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025534.
CleanExiMM_GUSB.
ExpressionAtlasiP12265. baseline and differential.
GenevisibleiP12265. MM.

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

Protein-protein interaction databases

IntActiP12265. 2 interactors.
MINTiMINT-4089235.
STRINGi10090.ENSMUSP00000026613.

Structurei

3D structure databases

ProteinModelPortaliP12265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2024. Eukaryota.
COG3250. LUCA.
GeneTreeiENSGT00390000001752.
HOGENOMiHOG000120896.
HOVERGENiHBG004843.
InParanoidiP12265.
KOiK01195.
OMAiINFDFFN.
OrthoDBiEOG091G02MO.
TreeFamiTF300685.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12265-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLKWSACWV ALGQLLCSCA LALKGGMLFP KESPSRELKA LDGLWHFRAD
60 70 80 90 100
LSNNRLQGFE QQWYRQPLRE SGPVLDMPVP SSFNDITQEA ALRDFIGWVW
110 120 130 140 150
YEREAILPRR WTQDTDMRVV LRINSAHYYA VVWVNGIHVV EHEGGHLPFE
160 170 180 190 200
ADISKLVQSG PLTTCRITIA INNTLTPHTL PPGTIVYKTD TSMYPKGYFV
210 220 230 240 250
QDTSFDFFNY AGLHRSVVLY TTPTTYIDDI TVITNVEQDI GLVTYWISVQ
260 270 280 290 300
GSEHFQLEVQ LLDEGGKVVA HGTGNQGQLQ VPSANLWWPY LMHEHPAYMY
310 320 330 340 350
SLEVKVTTTE SVTDYYTLPI GIRTVAVTKS KFLINGKPFY FQGVNKHEDS
360 370 380 390 400
DIRGKGFDWP LLVKDFNLLR WLGANSFRTS HYPYSEEVLQ LCDRYGIVVI
410 420 430 440 450
DECPGVGIVL PQSFGNESLR HHLEVMEELV RRDKNHPAVV MWSVANEPSS
460 470 480 490 500
ALKPAAYYFK TLITHTKALD LTRPVTFVSN AKYDADLGAP YVDVICVNSY
510 520 530 540 550
FSWYHDYGHL EVIQPQLNSQ FENWYKTHQK PIIQSEYGAD AIPGIHEDPP
560 570 580 590 600
RMFSEEYQKA VLENYHSVLD QKRKEYVVGE LIWNFADFMT NQSPLRVIGN
610 620 630 640
KKGIFTRQRQ PKTSAFILRE RYWRIANETG GHGSGPRTQC FGSRPFTF
Length:648
Mass (Da):74,195
Last modified:October 3, 2012 - v2
Checksum:iCCD8F84C3CD6C498
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265G → D in AAA37696 (PubMed:3397060).Curated1
Sequence conflicti265G → D in AAA98623 (PubMed:3196706).Curated1
Sequence conflicti265G → D in AAA63309 (PubMed:2779578).Curated1
Sequence conflicti265G → D in AAA37697 (PubMed:2835664).Curated1
Sequence conflicti320I → V in AAA37696 (PubMed:3397060).Curated1
Sequence conflicti320I → V in AAA98623 (PubMed:3196706).Curated1
Sequence conflicti320I → V in AAA37697 (PubMed:2835664).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti87T → I in strain: C3H/HeJ. 1
Natural varianti233I → T in allele GUS-SA. 1
Natural varianti428E → K in allele GUS-SA. 1
Natural varianti616F → L in allele GUS-SA. 1
Natural varianti642G → R in allele W26; reduced retention in the endoplasmic reticulum. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03047 mRNA. Translation: AAA37696.1.
J02836 Genomic DNA. Translation: AAA98623.1.
M63836 mRNA. Translation: AAA63309.1.
M28540 mRNA. Translation: AAA63307.1.
M28541 mRNA. Translation: AAA63308.1.
M19279 mRNA. Translation: AAA37697.1.
AK136519 mRNA. Translation: BAE23021.1.
AK150048 mRNA. Translation: BAE29265.1.
AK159526 mRNA. Translation: BAE35155.1.
AK162436 mRNA. Translation: BAE36917.1.
AC161345 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19485.1.
BC071226 mRNA. Translation: AAH71226.1.
CCDSiCCDS19705.1.
PIRiA32576.
RefSeqiNP_034498.1. NM_010368.1.
UniGeneiMm.3317.

Genome annotation databases

EnsembliENSMUST00000026613; ENSMUSP00000026613; ENSMUSG00000025534.
GeneIDi110006.
KEGGimmu:110006.
UCSCiuc008ztt.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03047 mRNA. Translation: AAA37696.1.
J02836 Genomic DNA. Translation: AAA98623.1.
M63836 mRNA. Translation: AAA63309.1.
M28540 mRNA. Translation: AAA63307.1.
M28541 mRNA. Translation: AAA63308.1.
M19279 mRNA. Translation: AAA37697.1.
AK136519 mRNA. Translation: BAE23021.1.
AK150048 mRNA. Translation: BAE29265.1.
AK159526 mRNA. Translation: BAE35155.1.
AK162436 mRNA. Translation: BAE36917.1.
AC161345 Genomic DNA. No translation available.
CH466529 Genomic DNA. Translation: EDL19485.1.
BC071226 mRNA. Translation: AAH71226.1.
CCDSiCCDS19705.1.
PIRiA32576.
RefSeqiNP_034498.1. NM_010368.1.
UniGeneiMm.3317.

3D structure databases

ProteinModelPortaliP12265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP12265. 2 interactors.
MINTiMINT-4089235.
STRINGi10090.ENSMUSP00000026613.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

PTM databases

PhosphoSitePlusiP12265.
SwissPalmiP12265.

Proteomic databases

EPDiP12265.
PaxDbiP12265.
PeptideAtlasiP12265.
PRIDEiP12265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026613; ENSMUSP00000026613; ENSMUSG00000025534.
GeneIDi110006.
KEGGimmu:110006.
UCSCiuc008ztt.1. mouse.

Organism-specific databases

CTDi2990.
MGIiMGI:95872. Gusb.

Phylogenomic databases

eggNOGiKOG2024. Eukaryota.
COG3250. LUCA.
GeneTreeiENSGT00390000001752.
HOGENOMiHOG000120896.
HOVERGENiHBG004843.
InParanoidiP12265.
KOiK01195.
OMAiINFDFFN.
OrthoDBiEOG091G02MO.
TreeFamiTF300685.

Enzyme and pathway databases

ReactomeiR-MMU-2024096. HS-GAG degradation.
R-MMU-2160916. Hyaluronan uptake and degradation.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiGusb. mouse.
PROiP12265.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025534.
CleanExiMM_GUSB.
ExpressionAtlasiP12265. baseline and differential.
GenevisibleiP12265. MM.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR006103. Glyco_hydro_2_cat.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SUPFAMiSSF49303. SSF49303. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGLR_MOUSE
AccessioniPrimary (citable) accession number: P12265
Secondary accession number(s): Q61601
, Q64473, Q64474, Q6IR10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 3, 2012
Last modified: November 30, 2016
This is version 156 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.