Reviewed, UniProtKB/Swiss-Prot P12260 (F13A_BOVIN)

Names and origin

Protein names

Recommended name:
    Coagulation factor XIII A chain
      Short name=Coagulation factor XIIIa
    EC=2.3.2.13
Alternative name(s):
    Protein-glutamine gamma-glutamyltransferase A chain
    Transglutaminase A chain

Gene names

Name:	F13A1
Synonyms:	F13A

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	198 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Tetramer of two A chains and two B chains.
Subcellular location	Cytoplasm. Secreted. Note: Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
Post-translational modification	The activation peptide is released by thrombin.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Cytoplasm Secreted
Ligand	Calcium
Molecular function	Acyltransferase Transferase
PTM	Acetylation Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Propeptide	2 – 38	37	Activation peptide		PRO_0000033645
	Chain	39 – ›198	›160	Coagulation factor XIII A chain		PRO_0000351119
Sites
	Site	38 – 39	2	Cleavage; by thrombin; to produce active factor XIII-A
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
Experimental info
	Sequence conflict	25	1	N → D AA sequence Ref.2
	Non-terminal residue	198	1

Sequences

	Sequence		Length	Mass (Da)
	P12260-1 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: A47F7B33469B89A2	FASTA	198	22,745
	10 20 30 40 50 60 MSESSGTAFG GRRAIPPNTS NAAENDPPTV ELQGLVPRGF NPQDYLNVTN VHLFKERWDS 70 80 90 100 110 120 NKVDHHTDKY SNDKLIVRRG QSFYIQIDFN RPYDPTRDLF RVEYVIGLYP QENKGTYIPV 130 140 150 160 170 180 PLVSELQSGK WGAKVVMRED RSVRLSVQSS ADCIVGKFRM YVAVWTPYGV IRTSRNPETD 190 TYILFNPWCE EDAVYLEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	"Amino acid sequence of the peptide released from bovine factor XIII following activation by thrombin." Nakamura S., Iwanaga S., Suzuki T., Mikuni Y., Konishi K. Biochem. Biophys. Res. Commun. 58:250-256(1974) [PubMed: 4831071] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-38.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BT025350 mRNA. Translation: ABF57306.1.
IPI	IPI00697215.
PIR	A10933.
UniGene	Bt.19195
3D structure databases
HSSP	HSSP built from PDB template 1GGY based on UniProtKB P00488.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000007268. Bos taurus. [Contig view]
Phylogenomic databases
HOVERGEN	P12260.
Enzyme and pathway databases
BRENDA	2.3.2.13. 251.
Family and domain databases
InterPro	IPR013783. Ig-like_fold. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00868. Transglut_N. 1 hit. [Graphical view]
PROSITE	PS00547. TRANSGLUTAMINASES. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F13A_BOVIN

Accession

Primary (citable) accession number: P12260
Secondary accession number(s): Q1JPK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	September 23, 2008
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)