P12246 (SAMP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serum amyloid P-component
Short name=SAP

Gene names

Name:	Apcs
Synonyms:	Ptx2, Sap

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.
Subcellular location	Secreted.
Involvement in disease	SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits.
Sequence similarities	Belongs to the pentaxin family. Contains 1 pentaxin domain.

Ontologies

Keywords
Cellular component	Amyloid Secreted
Domain	Signal
Ligand	Calcium Lectin Metal-binding
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein complex assembly Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular matrix Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara protein complex Inferred from electronic annotation. Source: Compara
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 224	204	Serum amyloid P-component		PRO_0000023542
Regions
	Domain	21 – 224	204	Pentaxin
Sites
	Metal binding	78	1	Calcium 1 By similarity
	Metal binding	79	1	Calcium 1 By similarity
	Metal binding	156	1	Calcium 1 By similarity
	Metal binding	156	1	Calcium 2 By similarity
	Metal binding	157	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	158	1	Calcium 1 By similarity
	Metal binding	158	1	Calcium 2 By similarity
	Metal binding	168	1	Calcium 2 By similarity
Amino acid modifications
	Glycosylation	52	1	N-linked (GlcNAc...) Ref.7
	Disulfide bond	56 ↔ 115		By similarity
Experimental info
	Sequence conflict	151	1	I → V in AAA40092. Ref.3
	Sequence conflict	151	1	I → V in CAA32243. Ref.3
	Sequence conflict	154	1	G → R in CAA68488. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P12246 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: 1CDBC15F45E50DCA	FASTA	224	26,247
	10 20 30 40 50 60 MDKLLLWMFV FTSLLSEAFC QTDLKRKVFV FPRESETDHV KLIPHLEKPL QNFTLCFRTY 70 80 90 100 110 120 SDLSRSQSLF SYSVKGRDNE LLIYKEKVGE YSLYIGQSKV TVRGMEEYLS PVHLCTTWES 130 140 150 160 170 180 SSGIVEFWVN GKPWVKKSLQ REYTVKAPPS IVLGQEQDNY GGGFQRSQSF VGEFSDLYMW 190 200 210 220 DYVLTPQDIL FVYRDSPVNP NILNWQALNY EINGYVVIRP RVWD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the mouse serum amyloid P component gene." Nishiguchi S., Maeda S., Araki S., Shimada K. Biochem. Biophys. Res. Commun. 155:1366-1373(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Characterization of the gene encoding mouse serum amyloid P component. Comparison with genes encoding other pentraxins." Whitehead A.S., Rits M. Biochem. J. 263:25-31(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: CBA/J.
[3]	"Isolation and analysis of murine serum amyloid P component cDNA clones." Mole J.E., Beaulieu B.L., Geheran C.A., Carnazza J.A., Anderson J.K. J. Immunol. 141:3642-3646(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2J. Tissue: Liver.
[4]	"The complete nucleotide and deduced amino acid sequence of mouse serum amyloid P component." Ishikawa N., Shigemoto K., Maruyama N. Nucleic Acids Res. 15:7186-7186(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NZB. Tissue: Liver.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[6]	"Molecular genetics of mouse serum amyloid P component (SAP): cloning and gene mapping." Whitehead A.S., Rits M., Michaelson J. Immunogenetics 28:388-390(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 159-224. Strain: CBA/J. Tissue: Liver.
[7]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-52, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23552 mRNA. Translation: AAA40092.1. M29535 Genomic DNA. Translation: AAA40093.1. X16899 Genomic DNA. Translation: CAA34774.1. X14079 mRNA. Translation: CAA32243.1. Y00426 mRNA. Translation: CAA68488.1. BC061125 mRNA. Translation: AAH61125.1. M23248 mRNA. Translation: AAA40091.1.
IPI	IPI00309214.
PIR	A30528.
RefSeq	NP_035448.2. NM_011318.2.
UniGene	Mm.330510.
3D structure databases
ProteinModelPortal	P12246.
SMR	P12246. Positions 21-223.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000027824.
PTM databases
PhosphoSite	P12246.
Proteomic databases
PaxDb	P12246.
PRIDE	P12246.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000027824; ENSMUSP00000027824; ENSMUSG00000026542.
GeneID	20219.
KEGG	mmu:20219.
Organism-specific databases
CTD	325.
MGI	MGI:98229. Apcs.
Phylogenomic databases
eggNOG	NOG44770.
HOGENOM	HOG000247043.
HOVERGEN	HBG005405.
InParanoid	P12246.
OMA	NPNILNW.
OrthoDB	EOG4R23VS.
Gene expression databases
ArrayExpress	P12246.
Bgee	P12246.
CleanEx	MM_APCS.
Genevestigator	P12246.
GermOnline	ENSMUSG00000026542. Mus musculus.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR001759. Pentaxin. [Graphical view]
Pfam	PF00354. Pentaxin. 1 hit. [Graphical view]
PRINTS	PR00895. PENTAXIN.
SMART	SM00159. PTX. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00289. PENTAXIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	297831.
SOURCE	Search...

Entry information

Entry name

SAMP_MOUSE

Accession

Primary (citable) accession number: P12246

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program