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Protein

Cytochrome b559 subunit alpha

Gene

psbE

Organism
Thermosynechococcus vulcanus (Synechococcus vulcanus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This b-type cytochrome is tightly associated with the reaction center of photosystem II (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.UniRule annotation2 Publications

Cofactori

heme bUniRule annotation4 PublicationsNote: With its partner (PsbF) binds heme. PSII binds additional chlorophylls, carotenoids and specific lipids.UniRule annotation4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231Iron (heme axial ligand); shared with beta subunit1 PublicationUniRule annotation2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b559 subunit alphaUniRule annotation
Alternative name(s):
PSII reaction center subunit VUniRule annotation
Gene namesi
Name:psbEUniRule annotation
OrganismiThermosynechococcus vulcanus (Synechococcus vulcanus)
Taxonomic identifieri32053 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2019Cytoplasmic1 PublicationAdd
BLAST
Transmembranei21 – 3515HelicalUniRule annotation1 PublicationAdd
BLAST
Topological domaini36 – 8449LumenalUniRule annotation1 PublicationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 8483Cytochrome b559 subunit alphaPRO_0000200346Add
BLAST

Interactioni

Subunit structurei

Heterodimer of an alpha subunit and a beta subunit. Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.UniRule annotation5 Publications

Protein-protein interaction databases

DIPiDIP-48862N.

Structurei

Secondary structure

1
84
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 145Combined sources
Helixi17 – 3923Combined sources
Helixi42 – 476Combined sources
Beta strandi54 – 563Combined sources
Helixi72 – 8110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IZLX-ray3.70E/P2-84[»]
3A0BX-ray3.70E/e3-84[»]
3A0HX-ray4.00E/e3-84[»]
3WU2X-ray1.90E/e2-84[»]
4IL6X-ray2.10E/e4-83[»]
4UB6X-ray1.95E/e1-84[»]
4UB8X-ray1.95E/e1-84[»]
ProteinModelPortaliP12238.
SMRiP12238. Positions 3-84.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12238.

Family & Domainsi

Sequence similaritiesi

Belongs to the PsbE/PsbF family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_00642. PSII_PsbE.
InterProiIPR006217. PSII_cyt_b559_asu.
IPR006216. PSII_cyt_b559_CS.
IPR013081. PSII_cyt_b559_N.
IPR013082. PSII_cytb559_asu_lum.
[Graphical view]
PfamiPF00283. Cytochrom_B559. 1 hit.
PF00284. Cytochrom_B559a. 1 hit.
[Graphical view]
PIRSFiPIRSF000036. PsbE. 1 hit.
TIGRFAMsiTIGR01332. cyt_b559_alpha. 1 hit.
PROSITEiPS00537. CYTOCHROME_B559. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12238-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGTTGERPF SDIITSVRYW VIHSITIPAL FIAGWLFVST GLAYDVFGTP
60 70 80
RPDSYYAQEQ RSIPLVTDRF EAKQQVETFL EQLK
Length:84
Mass (Da):9,573
Last modified:January 23, 2007 - v3
Checksum:i19C7A074624D937F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086860 Genomic DNA. Translation: BAC53634.1.
PIRiS05030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086860 Genomic DNA. Translation: BAC53634.1.
PIRiS05030.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IZLX-ray3.70E/P2-84[»]
3A0BX-ray3.70E/e3-84[»]
3A0HX-ray4.00E/e3-84[»]
3WU2X-ray1.90E/e2-84[»]
4IL6X-ray2.10E/e4-83[»]
4UB6X-ray1.95E/e1-84[»]
4UB8X-ray1.95E/e1-84[»]
ProteinModelPortaliP12238.
SMRiP12238. Positions 3-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48862N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12238.

Family and domain databases

HAMAPiMF_00642. PSII_PsbE.
InterProiIPR006217. PSII_cyt_b559_asu.
IPR006216. PSII_cyt_b559_CS.
IPR013081. PSII_cyt_b559_N.
IPR013082. PSII_cytb559_asu_lum.
[Graphical view]
PfamiPF00283. Cytochrom_B559. 1 hit.
PF00284. Cytochrom_B559a. 1 hit.
[Graphical view]
PIRSFiPIRSF000036. PsbE. 1 hit.
TIGRFAMsiTIGR01332. cyt_b559_alpha. 1 hit.
PROSITEiPS00537. CYTOCHROME_B559. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Low-molecular-mass polypeptide components of a photosystem II preparation from the thermophilic cyanobacterium Thermosynechococcus vulcanus."
    Kashino Y., Koike H., Yoshio M., Egashira H., Ikeuchi M., Pakrasi H.B., Satoh K.
    Plant Cell Physiol. 43:1366-1373(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8 AND 74-84, COMPOSITION OF PHOTOSYSTEM II, SUBUNIT.
  2. "Identification of psbI and psbL gene products in cyanobacterial photosystem II reaction center preparation."
    Ikeuchi M., Koike H., Inoue Y.
    FEBS Lett. 251:155-160(1989)
    Cited for: PROTEIN SEQUENCE OF 2-34.
  3. "Crystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7-A resolution."
    Kamiya N., Shen J.-R.
    Proc. Natl. Acad. Sci. U.S.A. 100:98-103(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.7 ANGSTROMS) OF 2-84 IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
  4. "Location of chloride and its possible functions in oxygen-evolving photosystem II revealed by X-ray crystallography."
    Kawakami K., Umena Y., Kamiya N., Shen J.R.
    Proc. Natl. Acad. Sci. U.S.A. 106:8567-8572(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.7 ANGSTROMS) OF 3-84 IN PHOTOSYSTEM II, FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
  5. "Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 A."
    Umena Y., Kawakami K., Shen J.R., Kamiya N.
    Nature 473:55-60(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 2-84 IN COMPLEX WITH HEME IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, TOPOLOGY.
  6. "Structure of Sr-substituted photosystem II at 2.1 A resolution and its implications in the mechanism of water oxidation."
    Koua F.H., Umena Y., Kawakami K., Shen J.R.
    Proc. Natl. Acad. Sci. U.S.A. 110:3889-3894(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 4-83 IN PHOTOSYSTEM II, FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPSBE_THEVL
AccessioniPrimary (citable) accession number: P12238
Secondary accession number(s): Q8GI50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: December 9, 2015
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.