P12236 (ADT3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADP/ATP translocase 3

Alternative name(s):
ADP,ATP carrier protein 3
ADP,ATP carrier protein, isoform T2
Short name=ANT 2
Adenine nucleotide translocator 3
Short name=ANT 3
Solute carrier family 25 member 6

Gene names

Name:	SLC25A6
Synonyms:	ANT3
ORF Names:	CDABP0051

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. Ref.6
Subunit structure	Found in a complex with ARL2, ARL2BP and SLC25A6 By similarity. Homodimer. Interacts with influenza A virus PB1-F2 protein and HIV-1 Vpr. Ref.7 Ref.8
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein. Note: The complex formed with ARL2BP, ARL2 and SLC25A6 is expressed in mitochondria By similarity.
Miscellaneous	The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue By similarity. The gene coding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.
Sequence similarities	Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification] Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Apoptosis Host-virus interaction Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Transmembrane Transmembrane helix
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	active induction of host immune response by virus Traceable author statement. Source: Reactome apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cellular protein metabolic process Traceable author statement. Source: Reactome energy reserve metabolic process Traceable author statement. Source: Reactome protein targeting to mitochondrion Traceable author statement. Source: Reactome regulation of insulin secretion Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome viral infectious cycle Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane presequence translocase complex Traceable author statement Ref.1. Source: UniProtKB
Molecular_function	ATP:ADP antiporter activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 298	297	ADP/ATP translocase 3		PRO_0000090584
Regions
	Transmembrane	5 – 39	35	Helical; Name=1; By similarity
	Transmembrane	75 – 100	26	Helical; Name=2; By similarity
	Transmembrane	109 – 143	35	Helical; Name=3; By similarity
	Transmembrane	176 – 202	27	Helical; Name=4; By similarity
	Transmembrane	207 – 241	35	Helical; Name=5; By similarity
	Transmembrane	273 – 298	26	Helical; Name=6; By similarity
	Repeat	6 – 98	93	Solcar 1
	Repeat	111 – 201	91	Solcar 2
	Repeat	212 – 297	86	Solcar 3
	Motif	235 – 240	6	Substrate recognition By similarity
Sites
	Binding site	80	1	Nucleotide By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylthreonine Ref.4
	Modified residue	23	1	N6-acetyllysine Ref.10
	Modified residue	105	1	N6-acetyllysine Ref.10
	Modified residue	268	1	N6-acetyllysine Ref.10
Natural variations
	Natural variant	242	1	S → F. Ref.2 Corresponds to variant rs17851235 [ dbSNP | Ensembl ].		VAR_054819
Experimental info
	Sequence conflict	105 – 108	4	KHTQ → RHA in AAA36750. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P12236 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 18534E9F0E49672F	FASTA	298	32,866
	10 20 30 40 50 60 MTEQAISFAK DFLAGGIAAA ISKTAVAPIE RVKLLLQVQH ASKQIAADKQ YKGIVDCIVR 70 80 90 100 110 120 IPKEQGVLSF WRGNLANVIR YFPTQALNFA FKDKYKQIFL GGVDKHTQFW RYFAGNLASG 130 140 150 160 170 180 GAAGATSLCF VYPLDFARTR LAADVGKSGT EREFRGLGDC LVKITKSDGI RGLYQGFSVS 190 200 210 220 230 240 VQGIIIYRAA YFGVYDTAKG MLPDPKNTHI VVSWMIAQTV TAVAGVVSYP FDTVRRRMMM 250 260 270 280 290 QSGRKGADIM YTGTVDCWRK IFRDEGGKAF FKGAWSNVLR GMGGAFVLVL YDELKKVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequences of two expressed nuclear genes for human mitochondrial ADP/ATP translocase." Cozens A.L., Runswick M.J., Walker J.E. J. Mol. Biol. 206:261-280(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Pediatric leukemia cDNA sequencing project." Zhou J., Yu W., Tang H., Mei G., Tsang Y.T.M., Bouck J., Gibbs R.A., Margolin J.F. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT PHE-242. Tissue: Leukemia.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Cervix, Eye and Lung.
[4]	Bienvenut W.V. Submitted (JUL-2004) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-10, ACETYLATION AT THR-2, MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[5]	"Two distinct genes for ADP/ATP translocase are expressed at the mRNA level in adult human liver." Houldsworth J., Attardi G. Proc. Natl. Acad. Sci. U.S.A. 85:377-381(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 36-298. Tissue: Liver.
[6]	"Study of PTPC composition during apoptosis for identification of viral protein target." Verrier F., Mignotte B., Jan G., Brenner C. Ann. N. Y. Acad. Sci. 1010:126-142(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN APOPTOSIS.
[7]	"Mitochondrial membrane permeabilization by HIV-1 Vpr." Deniaud A., Brenner C., Kroemer G. Mitochondrion 4:223-233(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HIV-1 VPR.
[8]	"Influenza virus PB1-F2 protein induces cell death through mitochondrial ANT3 and VDAC1." Zamarin D., Garcia-Sastre A., Xiao X., Wang R., Palese P. PLoS Pathog. 1:40-54(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH INFLUENZA A VIRUS PB1-F2.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[10]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-23; LYS-105 AND LYS-268, MASS SPECTROMETRY.
[11]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY007135 mRNA. Translation: AAG01998.1. BC007295 mRNA. Translation: AAH07295.1. BC007850 mRNA. Translation: AAH07850.1. BC008737 mRNA. Translation: AAH08737.1. BC008935 mRNA. Translation: AAH08935.1. BC014775 mRNA. Translation: AAH14775.1. BC031912 mRNA. Translation: AAH31912.1. J03592 mRNA. Translation: AAA36750.1.
IPI	IPI00291467.
PIR	S03894.
RefSeq	NP_001627.2. NM_001636.3.
UniGene	Hs.350927.
3D structure databases
ProteinModelPortal	P12236.
ModBase	Search...
Protein-protein interaction databases
IntAct	P12236. 35 interactions.
MINT	MINT-1154461.
STRING	9606.ENSP00000370808.
PTM databases
PhosphoSite	P12236.
Polymorphism databases
DMDM	113463.
Proteomic databases
PaxDb	P12236.
PeptideAtlas	P12236.
PRIDE	P12236.
Protocols and materials databases
DNASU	293.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000381401; ENSP00000370808; ENSG00000169100.
GeneID	293.
KEGG	hsa:293.
UCSC	uc004cpt.3. human.
Organism-specific databases
CTD	293.
GeneCards	GC0XM001505.
H-InvDB	HIX0034763.
HGNC	HGNC:10992. SLC25A6.
MIM	300151. gene. 403000. gene.
neXtProt	NX_P12236.
PharmGKB	PA35868.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG238123.
HOVERGEN	HBG108348.
InParanoid	P12236.
KO	K05863.
OMA	FNISCAG.
OrthoDB	EOG49CQ86.
PhylomeDB	P12236.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism. REACT_116125. Disease. REACT_17015. Metabolism of proteins.
Gene expression databases
ArrayExpress	P12236.
Bgee	P12236.
CleanEx	HS_SLC25A6.
Genevestigator	P12236.
GermOnline	ENSG00000169100. Homo sapiens.
Family and domain databases
Gene3D	1.50.40.10. 1 hit.
InterPro	IPR002113. Aden_trnslctor. IPR002067. Mit_carrier. IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
PRINTS	PR00927. ADPTRNSLCASE. PR00926. MITOCARRIER.
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00720. Clodronate.
GenomeRNAi	293.
NextBio	1195.
SOURCE	Search...

Entry information

Entry name

ADT3_HUMAN

Accession

Primary (citable) accession number: P12236
Secondary accession number(s): Q96C49

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 156 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.