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Protein

Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic

Gene

accD

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the acetyl coenzyme A carboxylase (ACC) complex. Biotin carboxylase (BC) catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO2 group is transferred by the transcarboxylase to acetyl-CoA to form malonyl-CoA.UniRule annotation

Miscellaneous

The protein in tobacco runs as 53 kDa; thus this may not be the correct start site.

Catalytic activityi

ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: malonyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes malonyl-CoA from acetyl-CoA.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD), Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD)
This subpathway is part of the pathway malonyl-CoA biosynthesis, which is itself part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes malonyl-CoA from acetyl-CoA, the pathway malonyl-CoA biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi230ZincUniRule annotation1
Metal bindingi233ZincUniRule annotation1
Metal bindingi249ZincUniRule annotation1
Metal bindingi252ZincUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri230 – 252C4-typeUniRule annotationAdd BLAST23

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00655; UER00711.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplasticUniRule annotation (EC:6.4.1.2UniRule annotation)
Short name:
ACCase subunit betaUniRule annotation
Short name:
Acetyl-CoA carboxylase carboxyltransferase subunit betaUniRule annotation
Gene namesi
Name:accDUniRule annotation
Synonyms:ycf11, zfpA
Encoded oniPlastid; Chloroplast
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

  • Plastidchloroplast stroma UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Biotechnological usei

Overexpression of the accD operon increases the specific activity of acetyl-coenzyme A carboxylase, increases leaf longevity and seed production. The accD operon consists of accD, psaI, ycf4, cemA, and petA in this order.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001997961 – 495Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplasticAdd BLAST495

Expressioni

Tissue specificityi

RNA expressed in leaf, root and stem; the least expression occurs in stems.1 Publication

Interactioni

Subunit structurei

Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein, biotin carboxylase and 2 subunits each of ACCase subunit alpha and ACCase plastid-coded subunit beta (accD).By similarity

Structurei

3D structure databases

SMRiP12219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini226 – 495CoA carboxyltransferase N-terminalPROSITE-ProRule annotationAdd BLAST270

Sequence similaritiesi

Belongs to the AccD/PCCB family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri230 – 252C4-typeUniRule annotationAdd BLAST23

Keywords - Domaini

Zinc-finger

Family and domain databases

HAMAPiMF_01395. AcetylCoA_CT_beta. 1 hit.
InterProiView protein in InterPro
IPR034733. AcCoA_carboxyl.
IPR000438. Acetyl_CoA_COase_Trfase_b_su.
IPR029045. ClpP/crotonase-like_dom.
IPR011762. COA_CT_N.
PfamiView protein in Pfam
PF01039. Carboxyl_trans. 1 hit.
PRINTSiPR01070. ACCCTRFRASEB.
SUPFAMiSSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR00515. accD. 1 hit.
PROSITEiView protein in PROSITE
PS50980. COA_CT_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

P12219-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERWWFNSML FKKEFERRCG LNKSMGSLGP IENTNEDPNR KVKNIHSWRN
60 70 80 90 100
RDNSSCSNVD YLFGVKDIRN FISDDTFLVS DRNGDSYSIY FDIENHIFEI
110 120 130 140 150
DNDHSFLSEL ESSFYSYRNS NYRNNGFRGE DPYYNSYMYD TQYSWNNHIN
160 170 180 190 200
SCIDSYLQSQ ICIDTSIISG SENYGDSYIY RAVCGGESRN SSENEGSSRR
210 220 230 240 250
TRTKGSDLTI RESSNDLEVT QKYRHLWVQC ENCYGLNYKK FLKSKMNICE
260 270 280 290 300
QCGYHLKMSS SDRIELLIDP GTWDPMDEDM VSLDPIEFHS EEEPYKDRID
310 320 330 340 350
SYQRKTGLTE AVQTGIGQLN GIPVAIGVMD FQFMGGSMGS VVGEKITRLI
360 370 380 390 400
EYAANQILPL IIVCASGGAR MQEGSLSLMQ MAKISSALYD YQLNKKLFYV
410 420 430 440 450
SILTSPTTGG VTASFGMLGD IIIAEPNAYI AFAGKRVIEQ TLNKTVPEGS
460 470 480 490
QAAEYLFQKG LFDLIVPRNL LKSVLSELFK LHAFFPLNQK SSKIK
Length:495
Mass (Da):56,459
Last modified:January 20, 2009 - v2
Checksum:iBC468ABFFDCAF24E
GO

Sequence cautioni

The sequence CAA77362 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z00044 Genomic DNA. Translation: CAA77362.1. Different initiation.
PIRiA05196.
RefSeqiNP_054508.1. NC_001879.2.

Genome annotation databases

GeneIDi800510.
KEGGinta:800510.

Similar proteinsi

Entry informationi

Entry nameiACCD_TOBAC
AccessioniPrimary (citable) accession number: P12219
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 20, 2009
Last modified: May 10, 2017
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families