Reviewed, UniProtKB/Swiss-Prot P12124 (NU1C_ORYSJ)

Names and origin

Protein names

Recommended name:
    NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic
    EC=1.6.5.-
Alternative name(s):
    NAD(P)H dehydrogenase subunit 1
      Short name=NDH subunit 1
    NADH-plastoquinone oxidoreductase subunit 1

Gene names

Name:	ndhA
ORF Names:	Nip174

Encoded on

Plastid; Chloroplast

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01350
Subunit structure	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 1 family.
RNA editing	Edited at positions 158, 188 and 357. By similarity. HAMAP MF_01350

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Thylakoid
Coding sequence diversity	RNA editing
Domain	Transmembrane
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic HAMAP MF_01350		PRO_0000117514
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	128 – 148	21	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	202 – 222	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	335 – 355	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P12124-1 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 8C459851391B8266	FASTA	362	40,468
	10 20 30 40 50 60 MIIDRVQVEA INSFSNLELL KEVYGLIWIL PILTLLLGIT IEVLVIVWLE REISASIQQR 70 80 90 100 110 120 IGPEYAGPLG LLQAIADGTK LLFKEDILPS RGDIPLFSIG PSIAVISILL SFLVIPLGYR 130 140 150 160 170 180 FVLADLSIGV FLWIAISSIA PIGLLMAGYS SNNKYSFLGG LRAAAQSISY EIPLTFCVLA 190 200 210 220 230 240 ISLLSNSLST VDIVEAQSKY GFFGWNLWRQ PIGFLVFLIS SLAECERLPF DLPEAEEELV 250 260 270 280 290 300 AGYQTEYSGI KYGLFYLVSY LNLLVSSLFV TVLYLGGWNL SIPYISFFGF FQMNKMVGIL 310 320 330 340 350 360 EMTMSIFITL TKAYLFLFIS ITIRWTLPRM RMDQLLNLGW KFLLPISLGN LLLTTSFQLV SL

References

[1]

"The complete sequence of the rice (Oryza sativa) chloroplast genome: intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals." Hiratsuka J., Shimada H., Whittier R., Ishibashi T., Sakamoto M., Mori M., Kondo C., Honji Y., Sun C.-R., Meng B.-Y., Li Y.-Q., Kanno A., Nishizawa Y., Hirai A., Shinozaki K., Sugiura M. Mol. Gen. Genet. 217:185-194(1989) [PubMed: 2770692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"A comparison of rice chloroplast genomes." Tang J., Xia H., Cao M., Zhang X., Zeng W., Hu S., Tong W., Wang J., Wang J., Yu J., Yang H., Zhu L. Plant Physiol. 135:412-420(2004) [PubMed: 15122023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15901 Genomic DNA. Translation: CAA33910.1. AY522330 Genomic DNA. No translation available.
PIR	DERZN1. JQ0293.
RefSeq	NP_039449.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3131395.
KEGG	osa:3131395.
Organism-specific databases
Gramene	P12124.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NU1C_ORYSJ

Accession

Primary (citable) accession number: P12124

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	June 27, 2006
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)