P12105 (CO3A1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Collagen alpha-1(III) chain

Gene names

Name:

COL3A1

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1262 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Collagen type III occurs in most soft connective tissues along with type I collagen.
Subunit structure	Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity.
Domain	The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function By similarity.
Post-translational modification	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sequence similarities	Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Cellular component	Extracellular matrix Secreted
Domain	Collagen Repeat Signal
Ligand	Calcium Metal-binding
PTM	Disulfide bond Glycoprotein Hydroxylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	collagen Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	extracellular matrix structural constituent Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 150	127	N-terminal propeptide By similarity		PRO_0000005737
	Chain	151 – 1017	867	Collagen alpha-1(III) chain		PRO_0000005738
	Propeptide	1018 – 1262	245	C-terminal propeptide By similarity		PRO_0000005739
Regions
	Domain	29 – 88	60	VWFC
	Domain	1028 – 1262	235	Fibrillar collagen NC1
	Region	145 – 164	20	Nonhelical region (N-terminal)
	Region	165 – 994	830	Triple-helical region
	Region	995 – 1003	9	Nonhelical region (C-terminal)
Sites
	Metal binding	1076	1	Calcium By similarity
	Metal binding	1078	1	Calcium By similarity
	Metal binding	1079	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	1081	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	1084	1	Calcium By similarity
Amino acid modifications
	Modified residue	262	1	5-hydroxylysine By similarity
	Modified residue	283	1	5-hydroxylysine By similarity
	Modified residue	859	1	5-hydroxylysine By similarity
	Glycosylation	1163	1	N-linked (GlcNAc...) Potential
	Disulfide bond	994		Interchain By similarity
	Disulfide bond	995		Interchain By similarity
	Disulfide bond	1058 ↔ 1090		By similarity
	Disulfide bond	1064		Interchain (with C-1285) By similarity
	Disulfide bond	1081		Interchain (with C-1268) By similarity
	Disulfide bond	1098 ↔ 1260		By similarity
	Disulfide bond	1168 ↔ 1213		By similarity
Experimental info
	Sequence conflict	96	1	E → K Ref.2
	Sequence conflict	881	1	G → R in CAA23689. Ref.3
	Sequence conflict	884	1	G → V in CAA23689. Ref.3
	Sequence conflict	984	1	L → V in CAA23690. Ref.3
	Sequence conflict	1132	1	F → S in AAA18519. Ref.4
	Non-adjacent residues	886 – 887	2
	Non-adjacent residues	922 – 923	2

Sequences

	Sequence		Length	Mass (Da)
	P12105 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 96ABE7B2E9DEB43D	FASTA	1,262	121,249
	10 20 30 40 50 60 MMSFVQKVSL FILAVFQPSV ILAQQDALGG CTHLGQEYAD RDVWKPEPCQ ICVCDSGSVL 70 80 90 100 110 120 CDDIICDDQE LDCPNPEIPL GECCPVCPQT TPQPTELPYT QGPKGDPGSP GSPGRTGAPG 130 140 150 160 170 180 PPGQPGSPGA PGPPGICQSC PSISGGSFSP QYDSYDVKAG SVGMGYPPQP ISGFPGPPGP 190 200 210 220 230 240 SGPPGPPGHA GPPGSNGYQG PPGEPGQPGP SGPPGPAGMI GPAGPPGKDG EPGRPGRNGD 250 260 270 280 290 300 RGIPGLPGHK GHPGMPGMPG MKGARGFDGK DGAKGDSGAP GPKGEAGQPG ANGSPGQPGP 310 320 330 340 350 360 GGPTGERGRP GNPGGPGAHG KDGAPGTAGP LGPPGPPGTA GFPGSPGFKG EAGPPGPAGA 370 380 390 400 410 420 SGNPGERGEP GPQGQAGPPG PQGPPGRAGS PGGKGEMGPS GIPGGPGPPG GRGLPGPPGT 430 440 450 460 470 480 SGNPGAKGTP GEPGKNGAKG DPGPKGERGE NGTPGARGPP GEEGKRGANG EPGQNGVPGT 490 500 510 520 530 540 PGERGSPGFR GLPGSNGLPG EKGPAGERGS PGPPGPSGPA GDRGQDGGPG LPGMRGLPGI 550 560 570 580 590 600 PGSPGSDGKP GPPGNQGEPG RSGPPGPAGP RGQPGVMGFP GPKGNEGAPG KNGERGPGGP 610 620 630 640 650 660 PGTPGPAGKN GDVGLPGPPG PAGPAGDRGE PGPSGSPGLQ GLPGGPGPAG ENGKPGEPGP 670 680 690 700 710 720 KGDIGGPGFP GPKGENGIPG ERGPQGPPGP TGARGGPGPA GSEGAKGPPG PPGAPGGTGL 730 740 750 760 770 780 PGLQGMPGER GASGSPGPKG DKGEPGGKGA DGLPGARGER GNVGPIGPPG PAGPPGDKGE 790 800 810 820 830 840 TGPAGAPGPA GSRGGPGERG EQGLPGPAGF PGAPGQNGEP GGKGERGPPG LRGEAGPPGA 850 860 870 880 890 900 AGPQGGPGAP GPPGPQGVKG ERGSPGGPGA AGFPGARGPP GPPGNNGDRG ESGPPGVPGP 910 920 930 940 950 960 PGHPGPAGNN GAPGKAGERG FQGPLGPQGA IGSPGASGAR GPPGPAGPPG KDGRGGYPGP 970 980 990 1000 1010 1020 IGPPGPRGNR GESGPAGPPG QPGLPGPSGP PGPCCGGGVA SLGAGEKGPV GYGYEYRDEP 1030 1040 1050 1060 1070 1080 KENEINLGEI MSSMKSINNQ IENILSPDGS RKNPARNCRD LKFCHPELKS GEYWIDPNQG 1090 1100 1110 1120 1130 1140 CKMDAIKVYC NMETGETCLS ANPATVPRKN WWTTESSGKK HVWFGESMKG GFQFSYGDPD 1150 1160 1170 1180 1190 1200 LPEDVSEVQL AFLRILSSRA SQNITYHCKN SIAYMNQASG NVKKALKLMS SVETDIKAEG 1210 1220 1230 1240 1250 1260 NSKYMYAVLE DGCTKHTGEW GKTVFEYRTR KTMRLPVVDI APIDIGGPDQ EFGVDVGPVC FL

References

[1]	"An alternative transcript of the chick type III collagen gene that does not encode type III collagen." Nah H.-D., Niu Z., Adams S.L. J. Biol. Chem. 269:16443-16448(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-886. Tissue: Kidney.
[2]	"Conservation of the sizes for one but not another class of exons in two chick collagen genes." Yamada Y., Liau G., Mudryj M., Obici S., de Crombrugghe B. Nature 310:333-337(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 29-96; 332-397; 431-484; 503-535 AND 869-976.
[3]	"Isolation and characterization of a genomic clone encoding chick alpha-1 type III collagen." Yamada Y., Mudryj M., Sullivan M., de Crombrugghe B. J. Biol. Chem. 258:2758-2761(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 869-886 AND 977-994.
[4]	"A conserved nucleotide sequence, coding for a segment of the C-propeptide, is found at the same location in different collagen genes." Yamada Y., Kuhn K., de Crombrugghe B. Nucleic Acids Res. 11:2733-2744(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 977-1262.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U07973 mRNA. Translation: AAA83407.1. X00822, X00823 Genomic DNA. Translation: CAB52686.1. X00826, X00825 Genomic DNA. Translation: CAA25397.1. Sequence problems. X00827 Genomic DNA. Translation: CAA25398.1. X00828 Genomic DNA. Translation: CAA25399.1. X00830 Genomic DNA. Translation: CAA25401.1. X00831 Genomic DNA. Translation: CAA25402.1. K02302 Genomic DNA. Translation: AAD15299.1. K02301 Genomic DNA. Translation: AAD15298.1. V00391 Genomic DNA. Translation: CAA23689.1. V00392 Genomic DNA. Translation: CAA23690.1. M36662 Unassigned DNA. Translation: AAA18519.1. Sequence problems.
IPI	IPI00590578.
PIR	A05269. I50694.
UniGene	Gga.42140.
3D structure databases
ProteinModelPortal	P12105.
ModBase	Search...
Proteomic databases
PaxDb	P12105.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOVERGEN	HBG004933.
Family and domain databases
InterPro	IPR008160. Collagen. IPR000885. Fib_collagen_C. IPR001007. VWF_C. [Graphical view]
Pfam	PF01410. COLFI. 1 hit. PF01391. Collagen. 7 hits. PF00093. VWC. 1 hit. [Graphical view]
ProDom	PD002078. Fib_collagen_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00038. COLFI. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
PROSITE	PS51461. NC1_FIB. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CO3A1_CHICK

Accession

Primary (citable) accession number: P12105
Secondary accession number(s): P79758 , P79759, Q90790, Q90791, Q90794, Q92029

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	August 29, 2001
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program