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Protein

Fatty acid-binding protein, intestinal

Gene

FABP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei83 – 831Fatty acidBy similarity
Binding sitei107 – 1071Fatty acidBy similarity

GO - Molecular functioni

  • fatty acid binding Source: ProtInc
  • transporter activity Source: InterPro

GO - Biological processi

  • digestion Source: ProtInc
  • small molecule metabolic process Source: Reactome
  • triglyceride catabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiREACT_494. Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid-binding protein, intestinal
Alternative name(s):
Fatty acid-binding protein 2
Intestinal-type fatty acid-binding protein
Short name:
I-FABP
Gene namesi
Name:FABP2
Synonyms:FABPI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:3556. FABP2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi39 – 391L → G: Reduced stability. 1 Publication
Mutagenesisi64 – 641E → G: Localized reduction in stability. 1 Publication
Mutagenesisi65 – 651L → A: Reduced stability. 1 Publication
Mutagenesisi65 – 651L → G: Reduced stability. 1 Publication
Mutagenesisi67 – 671V → G: Localized reduction in stability. 1 Publication
Mutagenesisi90 – 901L → G: Reduced stability. 1 Publication
Mutagenesisi123 – 1231V → G: Reduced stability. 1 Publication

Organism-specific databases

PharmGKBiPA27957.

Chemistry

DrugBankiDB00783. Estradiol.
DB01050. Ibuprofen.
DB01138. Sulfinpyrazone.

Polymorphism and mutation databases

BioMutaiFABP2.
DMDMi119805.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 132131Fatty acid-binding protein, intestinalPRO_0000067328Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP12104.
PRIDEiP12104.

PTM databases

PhosphoSiteiP12104.

Expressioni

Tissue specificityi

Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum.1 Publication

Inductioni

By EGF.1 Publication

Gene expression databases

BgeeiP12104.
CleanExiHS_FABP2.
GenevestigatoriP12104.

Organism-specific databases

HPAiCAB047325.
CAB047326.
HPA034607.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
AGR2O959943EBI-3905109,EBI-712648

Protein-protein interaction databases

BioGridi108467. 3 interactions.
IntActiP12104. 2 interactions.
STRINGi9606.ENSP00000274024.

Structurei

Secondary structure

1
132
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 1410Combined sources
Helixi15 – 228Combined sources
Helixi26 – 327Combined sources
Turni33 – 353Combined sources
Beta strandi38 – 447Combined sources
Beta strandi47 – 537Combined sources
Beta strandi58 – 647Combined sources
Beta strandi69 – 724Combined sources
Beta strandi74 – 763Combined sources
Beta strandi78 – 869Combined sources
Beta strandi89 – 968Combined sources
Turni97 – 993Combined sources
Beta strandi102 – 1109Combined sources
Beta strandi113 – 1208Combined sources
Beta strandi123 – 1319Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KZWNMR-A2-132[»]
1KZXNMR-A2-132[»]
2MJINMR-A2-132[»]
3AKMX-ray1.90A/B/C/D2-132[»]
3IFBNMR-A2-132[»]
ProteinModelPortaliP12104.
SMRiP12104. Positions 2-132.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12104.

Family & Domainsi

Domaini

Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG295259.
HOGENOMiHOG000004829.
HOVERGENiHBG005633.
InParanoidiP12104.
KOiK08751.
OrthoDBiEOG7NW6BZ.
PhylomeDBiP12104.
TreeFamiTF316894.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR000463. Fatty_acid-bd.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00178. FATTYACIDBP.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00214. FABP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12104-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFDSTWKVD RSENYDKFME KMGVNIVKRK LAAHDNLKLT ITQEGNKFTV
60 70 80 90 100
KESSAFRNIE VVFELGVTFN YNLADGTELR GTWSLEGNKL IGKFKRTDNG
110 120 130
NELNTVREII GDELVQTYVY EGVEAKRIFK KD
Length:132
Mass (Da):15,207
Last modified:January 23, 2007 - v2
Checksum:i68330E3D81792CAF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551A → T Found in 29% of the population; associated with increased plasma insulin concentration, increased fat oxidation and insulin resistance; 2-fold greater affinity for long-chain fatty acids. 4 Publications
Corresponds to variant rs1799883 [ dbSNP | Ensembl ].
VAR_002379

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18079 Genomic DNA. Translation: AAA52417.1.
AC092656 Genomic DNA. No translation available.
BC069466 mRNA. Translation: AAH69466.1.
BC069617 mRNA. Translation: AAH69617.1.
BC069625 mRNA. Translation: AAH69625.1.
BC069637 mRNA. Translation: AAH69637.1.
BC111791 mRNA. Translation: AAI11792.1.
CCDSiCCDS3712.1.
PIRiA29781. FZHUI.
RefSeqiNP_000125.2. NM_000134.3.
UniGeneiHs.282265.

Genome annotation databases

EnsembliENST00000274024; ENSP00000274024; ENSG00000145384.
GeneIDi2169.
KEGGihsa:2169.
UCSCiuc003icw.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18079 Genomic DNA. Translation: AAA52417.1.
AC092656 Genomic DNA. No translation available.
BC069466 mRNA. Translation: AAH69466.1.
BC069617 mRNA. Translation: AAH69617.1.
BC069625 mRNA. Translation: AAH69625.1.
BC069637 mRNA. Translation: AAH69637.1.
BC111791 mRNA. Translation: AAI11792.1.
CCDSiCCDS3712.1.
PIRiA29781. FZHUI.
RefSeqiNP_000125.2. NM_000134.3.
UniGeneiHs.282265.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KZWNMR-A2-132[»]
1KZXNMR-A2-132[»]
2MJINMR-A2-132[»]
3AKMX-ray1.90A/B/C/D2-132[»]
3IFBNMR-A2-132[»]
ProteinModelPortaliP12104.
SMRiP12104. Positions 2-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108467. 3 interactions.
IntActiP12104. 2 interactions.
STRINGi9606.ENSP00000274024.

Chemistry

BindingDBiP12104.
ChEMBLiCHEMBL4879.
DrugBankiDB00783. Estradiol.
DB01050. Ibuprofen.
DB01138. Sulfinpyrazone.

PTM databases

PhosphoSiteiP12104.

Polymorphism and mutation databases

BioMutaiFABP2.
DMDMi119805.

Proteomic databases

PaxDbiP12104.
PRIDEiP12104.

Protocols and materials databases

DNASUi2169.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000274024; ENSP00000274024; ENSG00000145384.
GeneIDi2169.
KEGGihsa:2169.
UCSCiuc003icw.3. human.

Organism-specific databases

CTDi2169.
GeneCardsiGC04M120238.
H-InvDBHIX0004469.
HGNCiHGNC:3556. FABP2.
HPAiCAB047325.
CAB047326.
HPA034607.
MIMi134640. gene.
neXtProtiNX_P12104.
PharmGKBiPA27957.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG295259.
HOGENOMiHOG000004829.
HOVERGENiHBG005633.
InParanoidiP12104.
KOiK08751.
OrthoDBiEOG7NW6BZ.
PhylomeDBiP12104.
TreeFamiTF316894.

Enzyme and pathway databases

ReactomeiREACT_494. Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis.

Miscellaneous databases

ChiTaRSiFABP2. human.
EvolutionaryTraceiP12104.
GeneWikiiFABP2.
GenomeRNAii2169.
NextBioi8759.
PROiP12104.
SOURCEiSearch...

Gene expression databases

BgeeiP12104.
CleanExiHS_FABP2.
GenevestigatoriP12104.

Family and domain databases

Gene3Di2.40.128.20. 1 hit.
InterProiIPR012674. Calycin.
IPR011038. Calycin-like.
IPR000463. Fatty_acid-bd.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamiPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSiPR00178. FATTYACIDBP.
SUPFAMiSSF50814. SSF50814. 1 hit.
PROSITEiPS00214. FABP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The human and rodent intestinal fatty acid binding protein genes. A comparative analysis of their structure, expression, and linkage relationships."
    Sweetser D.A., Birkenmeier E.H., Klisak I.J., Zollman S., Sparkes R.S., Mohandas T., Lusis A.J., Gordon J.I.
    J. Biol. Chem. 262:16060-16071(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-55.
  4. "Intestinal-type and liver-type fatty acid-binding protein in the intestine. Tissue distribution and clinical utility."
    Pelsers M.M.A.L., Namiot Z., Kisielewski W., Namiot A., Januszkiewicz M., Hermens W.T., Glatz J.F.C.
    Clin. Biochem. 36:529-535(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells."
    Darimont C., Gradoux N., de Pover A.
    Am. J. Physiol. 276:G606-G612(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY EGF.
  6. "Consequences of single-site mutations in the intestinal fatty acid binding protein."
    Rajabzadeh M., Kao J., Frieden C.
    Biochemistry 42:12192-12199(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LEU-39; GLU-64; LEU-65; VAL-67; LEU-90 AND VAL-123.
  7. "Solution structure of human intestinal fatty acid binding protein: implications for ligand entry and exit."
    Zhang F., Luecke C., Baier L.J., Sacchettini J.C., Hamilton J.A.
    J. Biomol. NMR 9:213-228(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
    Tissue: Intestine.
  8. "Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism."
    Zhang F., Luecke C., Baier L.J., Sacchettini J.C., Hamilton J.A.
    Biochemistry 42:7339-7347(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF VARIANT THR-55.
  9. "An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increased fat oxidation, and insulin resistance."
    Baier L.J., Sacchettini J.C., Knowler W.C., Eads J., Paolisso G., Tataranni P.A., Mochizuki H., Bennett P.H., Bogardus C., Prochazka M.
    J. Clin. Invest. 95:1281-1287(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT THR-55.
  10. "The association of Ala54Thr variant of intestinal fatty acid binding protein gene with general and regional adipose tissue depots."
    Kunsan X., Taisan Z., Weiping J., Duoqi S., Wei D., Jie L., Junxi L., Rong Z.
    Chin. Med. Sci. J. 14:46-51(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT THR-55.

Entry informationi

Entry nameiFABPI_HUMAN
AccessioniPrimary (citable) accession number: P12104
Secondary accession number(s): Q2NKJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: May 27, 2015
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.