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P12104 (FABPI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fatty acid-binding protein, intestinal
Alternative name(s):
Fatty acid-binding protein 2
Intestinal-type fatty acid-binding protein
Short name=I-FABP
Gene names
Name:FABP2
Synonyms:FABPI
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length132 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

Subcellular location

Cytoplasm.

Tissue specificity

Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. Ref.4

Induction

By EGF. Ref.5

Domain

Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.

Sequence similarities

Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCytoplasm
   Coding sequence diversityPolymorphism
   LigandLipid-binding
   PTMAcetylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdigestion

Traceable author statement Ref.9. Source: ProtInc

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfatty acid binding

Traceable author statement Ref.1. Source: ProtInc

transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 132131Fatty acid-binding protein, intestinal
PRO_0000067328

Sites

Binding site831Fatty acid By similarity
Binding site1071Fatty acid By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Natural variations

Natural variant551A → T in 29% of the population; 2-fold greater affinity for long-chain fatty acids; increased fat oxidation and insulin resistance. Ref.3 Ref.8 Ref.9 Ref.10
Corresponds to variant rs1799883 [ dbSNP | Ensembl ].
VAR_002379

Experimental info

Mutagenesis391L → G: Reduced stability. Ref.6
Mutagenesis641E → G: Localized reduction in stability. Ref.6
Mutagenesis651L → A: Reduced stability. Ref.6
Mutagenesis651L → G: Reduced stability. Ref.6
Mutagenesis671V → G: Localized reduction in stability. Ref.6
Mutagenesis901L → G: Reduced stability. Ref.6
Mutagenesis1231V → G: Reduced stability. Ref.6

Secondary structure

............................ 132
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P12104 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 68330E3D81792CAF

FASTA13215,207
        10         20         30         40         50         60 
MAFDSTWKVD RSENYDKFME KMGVNIVKRK LAAHDNLKLT ITQEGNKFTV KESSAFRNIE 

        70         80         90        100        110        120 
VVFELGVTFN YNLADGTELR GTWSLEGNKL IGKFKRTDNG NELNTVREII GDELVQTYVY 

       130 
EGVEAKRIFK KD 

« Hide

References

« Hide 'large scale' references
[1]"The human and rodent intestinal fatty acid binding protein genes. A comparative analysis of their structure, expression, and linkage relationships."
Sweetser D.A., Birkenmeier E.H., Klisak I.J., Zollman S., Sparkes R.S., Mohandas T., Lusis A.J., Gordon J.I.
J. Biol. Chem. 262:16060-16071(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. expand/collapse author list , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-55.
[4]"Intestinal-type and liver-type fatty acid-binding protein in the intestine. Tissue distribution and clinical utility."
Pelsers M.M.A.L., Namiot Z., Kisielewski W., Namiot A., Januszkiewicz M., Hermens W.T., Glatz J.F.C.
Clin. Biochem. 36:529-535(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells."
Darimont C., Gradoux N., de Pover A.
Am. J. Physiol. 276:G606-G612(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY EGF.
[6]"Consequences of single-site mutations in the intestinal fatty acid binding protein."
Rajabzadeh M., Kao J., Frieden C.
Biochemistry 42:12192-12199(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF LEU-39; GLU-64; LEU-65; VAL-67; LEU-90 AND VAL-123.
[7]"Solution structure of human intestinal fatty acid binding protein: implications for ligand entry and exit."
Zhang F., Luecke C., Baier L.J., Sacchettini J.C., Hamilton J.A.
J. Biomol. NMR 9:213-228(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR.
Tissue: Intestine.
[8]"Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism."
Zhang F., Luecke C., Baier L.J., Sacchettini J.C., Hamilton J.A.
Biochemistry 42:7339-7347(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF VARIANT THR-55.
[9]"An amino acid substitution in the human intestinal fatty acid binding protein is associated with increased fatty acid binding, increased fat oxidation, and insulin resistance."
Baier L.J., Sacchettini J.C., Knowler W.C., Eads J., Paolisso G., Tataranni P.A., Mochizuki H., Bennett P.H., Bogardus C., Prochazka M.
J. Clin. Invest. 95:1281-1287(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT THR-55.
[10]"The association of Ala54Thr variant of intestinal fatty acid binding protein gene with general and regional adipose tissue depots."
Kunsan X., Taisan Z., Weiping J., Duoqi S., Wei D., Jie L., Junxi L., Rong Z.
Chin. Med. Sci. J. 14:46-51(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT THR-55.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M18079 Genomic DNA. Translation: AAA52417.1.
AC092656 Genomic DNA. No translation available.
BC069466 mRNA. Translation: AAH69466.1.
BC069617 mRNA. Translation: AAH69617.1.
BC069625 mRNA. Translation: AAH69625.1.
BC069637 mRNA. Translation: AAH69637.1.
BC111791 mRNA. Translation: AAI11792.1.
PIRFZHUI. A29781.
RefSeqNP_000125.2. NM_000134.3.
UniGeneHs.282265.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KZWNMR-A2-132[»]
1KZXNMR-A2-132[»]
3AKMX-ray1.90A/B/C/D2-132[»]
3IFBNMR-A2-132[»]
ProteinModelPortalP12104.
SMRP12104. Positions 2-132.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid108467. 2 interactions.
IntActP12104. 1 interaction.
STRING9606.ENSP00000274024.

Chemistry

BindingDBP12104.
ChEMBLCHEMBL4879.

PTM databases

PhosphoSiteP12104.

Polymorphism databases

DMDM119805.

Proteomic databases

PaxDbP12104.
PRIDEP12104.

Protocols and materials databases

DNASU2169.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000274024; ENSP00000274024; ENSG00000145384.
GeneID2169.
KEGGhsa:2169.
UCSCuc003icw.3. human.

Organism-specific databases

CTD2169.
GeneCardsGC04M120238.
H-InvDBHIX0004469.
HGNCHGNC:3556. FABP2.
HPACAB047325.
CAB047326.
HPA034607.
MIM134640. gene.
neXtProtNX_P12104.
PharmGKBPA27957.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG295259.
HOGENOMHOG000004829.
HOVERGENHBG005633.
InParanoidP12104.
KOK08751.
OrthoDBEOG7NW6BZ.
PhylomeDBP12104.
TreeFamTF316894.

Gene expression databases

BgeeP12104.
CleanExHS_FABP2.
GenevestigatorP12104.

Family and domain databases

Gene3D2.40.128.20. 1 hit.
InterProIPR012674. Calycin.
IPR011038. Calycin-like.
IPR000463. Fatty_acid-bd.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]
PfamPF00061. Lipocalin. 1 hit.
[Graphical view]
PRINTSPR00178. FATTYACIDBP.
SUPFAMSSF50814. SSF50814. 1 hit.
PROSITEPS00214. FABP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFABP2. human.
EvolutionaryTraceP12104.
GeneWikiFABP2.
GenomeRNAi2169.
NextBio8759.
PROP12104.
SOURCESearch...

Entry information

Entry nameFABPI_HUMAN
AccessionPrimary (citable) accession number: P12104
Secondary accession number(s): Q2NKJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM