P12081 (SYHC_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine--tRNA ligase, cytoplasmic
EC=6.1.1.21

Alternative name(s):
Histidyl-tRNA synthetase
Short name=HisRS

Gene names

Name:	HARS
Synonyms:	HRS

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His).
Subcellular location	Cytoplasm.
Tissue specificity	Brain, heart, liver and kidney.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.
Sequence caution	The sequence CAA28956.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	ATP binding Non-traceable author statement. Source: UniProtKB histidine-tRNA ligase activity Non-traceable author statement Ref.1Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 509

508

Histidine--tRNA ligase, cytoplasmic

PRO_0000136332

Regions

Domain

3 – 59

57

WHEP-TRS

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.6

Modified residue

443

1

N6-acetyllysine Ref.7

Natural variations

Natural variant

399

1

A → V.
Corresponds to variant rs34732372 [ dbSNP | Ensembl ].

VAR_061908

Experimental info

Sequence conflict

6

1

A → P in CAA28956. Ref.2

Sequence conflict

165

1

R → G in CAA28956. Ref.2

Sequence conflict

181

1

N → Q in CAA28956. Ref.2

Sequence conflict

186

1

I → N in CAA28956. Ref.2

Sequence conflict

191

1

C → S in CAA28956. Ref.2

Sequence conflict

206

1

D → N in CAA28956. Ref.2

Sequence conflict

223

1

I → V in CAA28956. Ref.2

Sequence conflict

227

1

S → P in CAA28956. Ref.2

Sequence conflict

284

1

L → V in CAA28956. Ref.2

Sequence conflict

347

1

A → E in CAA28956. Ref.2

Sequence conflict

373 – 374

2

KG → QR in CAA28956. Ref.2

Sequence conflict

493

1

D → E in CAA28956. Ref.2

Secondary structure

1

.

.

.

.

.

509

Helix Strand Turn

Details...

Helix

2 – 23

22

Helix

28 – 45

18

Sequences

	Sequence		Length	Mass (Da)
	P12081 [UniParc]. Last modified May 1, 1992. Version 2. Checksum: 65D8BB71CE79B1FF	FASTA	509	57,411
	10 20 30 40 50 60 MAERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK 70 80 90 100 110 120 GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK ETLMGKYGED SKLIYDLKDQ 130 140 150 160 170 180 GGELLSLRYD LTVPFARYLA MNKLTNIKRY HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG 190 200 210 220 230 240 NFDPMIPDAE CLKIMCEILS SLQIGDFLVK VNDRRILDGM FAICGVSDSK FRTICSSVDK 250 260 270 280 290 300 LDKVSWEEVK NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG 310 320 330 340 350 360 DLKLLFEYLT LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQTPAQAGEE PLGVGSVAAG 370 380 390 400 410 420 GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL EEKIRTTETQ VLVASAQKKL 430 440 450 460 470 480 LEERLKLVSE LWDAGIKAEL LYKKNPKLLN QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS 490 500 VTSREEVDVR REDLVEEIKR RTGQPLCIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human histidyl-tRNA synthetase: recognition of amino acid signature regions in class 2a aminoacyl-tRNA synthetases." Raben N., Borriello F., Amin J., Horwitz R., Fraser D., Plotz P. Nucleic Acids Res. 20:1075-1081(1992) [PubMed: 1549469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation, structure and expression of mammalian genes for histidyl-tRNA synthetase." Tsui F.W.L., Siminovitch L. Nucleic Acids Res. 15:3349-3367(1987) [PubMed: 3554142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Uterus.
[4]	"Transcriptional analyses of the gene region that encodes human histidyl-tRNA synthetase: identification of a novel bidirectional regulatory element." Tsui H.W., Mok S., de Souza L., Martin A., Tsui F.W.L. Gene 131:201-208(1993) [PubMed: 8406012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
[5]	"A novel gene oriented in a head-to-head configuration with the human histidyl-tRNA synthetase (HRS) gene encodes an mRNA that predicts a polypeptide homologous to HRS." O'Hanlon T.P., Raben N., Miller F.W. Biochem. Biophys. Res. Commun. 210:556-566(1995) [PubMed: 7755634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-443, MASS SPECTROMETRY.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Solution structures of the WHEP-TRS domain of human histidyl-tRNA synthetase." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2005) to the PDB data bank Cited for: STRUCTURE BY NMR OF 1-60.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z11518 mRNA. Translation: CAA77607.1.
X05345 mRNA. Translation: CAA28956.1. Frameshift.
BC011807 mRNA. Translation: AAH11807.1.
BC080514 mRNA. Translation: AAH80514.1.
M96646 Genomic DNA. Translation: AAA58668.1.
U18936 Genomic DNA. Translation: AAA73973.1.

IPI

IPI00021808.

PIR

SYHUHT. I37559.

RefSeq

NP_002100.2. NM_002109.3.

UniGene

Hs.528050.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1X59	NMR	-	A	1-60	[»]

ProteinModelPortal

P12081.

SMR

P12081. Positions 1-502.

ModBase

Search...

Protein-protein interaction databases

IntAct

P12081. 1 interaction.

STRING

P12081.

PTM databases

PhosphoSite

P12081.

Polymorphism databases

DMDM

135123.

Proteomic databases

PeptideAtlas

P12081.

PRIDE

P12081.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000307633; ENSP00000304668; ENSG00000170445.

GeneID

3035.

KEGG

hsa:3035.

NMPDR

fig|9606.3.peg.25876.

UCSC

uc003lgu.1. human.

Organism-specific databases

CTD

3035.

GeneCards

GC05M140034.

H-InvDB

HIX0005239.

HGNC

HGNC:4816. HARS.

HPA

HPA036539.

MIM

142810. gene.

neXtProt

NX_P12081.

PharmGKB

PA29191.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG19383.

GeneTree

ENSGT00390000005922.

HOGENOM

HBG616575.

HOVERGEN

HBG002731.

InParanoid

P12081.

OMA

LVSELWD.

PhylomeDB

P12081.

Enzyme and pathway databases

BRENDA

6.1.1.21. 2681.

Reactome

REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

P12081.

Bgee

P12081.

CleanEx

HS_HARS.

Genevestigator

P12081.

GermOnline

ENSG00000170445. Homo sapiens.

Family and domain databases

InterPro

IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR015807. His-tRNA-synth_IIa_subgr.
IPR004516. His-tRNA_synth_IIA.
IPR009068. S15_NS1_RNA-bd.
IPR000738. WHEP-TRS.
[Graphical view]

Gene3D

G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
G3DSA:1.10.287.10. S15_NS1_RNA_bd. 1 hit.

KO

K01892.

PANTHER

PTHR11476. His-tRNA_synth. 1 hit.

Pfam

PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]

PIRSF

PIRSF001549. His-tRNA_synth. 1 hit.

SMART

SM00991. WHEP-TRS. 1 hit.
[Graphical view]

SUPFAM

SSF52954. Anticodon_bd. 1 hit.
SSF47060. S15/NS1_bind. 1 hit.

TIGRFAMs

TIGR00442. HisS. 1 hit.

PROSITE

PS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00117. L-Histidine.

NextBio

12014.

SOURCE

Search...

Entry information

Entry name

SYHC_HUMAN

Accession

Primary (citable) accession number: P12081

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	May 1, 1992
Last modified:	January 25, 2012
This is version 128 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.