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Protein

Trypsin inhibitor 2

Gene
N/A
Organism
Ecballium elaterium (Jumping cucumber) (Momordica elaterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits trypsin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei4 – 52Reactive bond

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI07.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin inhibitor 2
Alternative name(s):
EETI-II
Trypsin inhibitor II
OrganismiEcballium elaterium (Jumping cucumber) (Momordica elaterium)
Taxonomic identifieri3679 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBryonieaeEcballium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 3030Trypsin inhibitor 2PRO_0000044381Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi2 ↔ 19
Disulfide bondi9 ↔ 21
Disulfide bondi15 ↔ 27

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
30
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Helixi12 – 143Combined sources
Beta strandi25 – 284Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H9HX-ray1.50I1-30[»]
1H9IX-ray1.90I1-30[»]
1W7ZX-ray1.67A/B/C/D/E/F/G/H1-30[»]
2C4BX-ray1.30A/B16-29[»]
2ETINMR-A1-28[»]
2IT7NMR-A1-28[»]
2LETNMR-A1-28[»]
ProteinModelPortaliP12071.
SMRiP12071. Positions 2-29.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12071.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamiPF00299. Squash. 1 hit.
[Graphical view]
PRINTSiPR00293. SQUASHINHBTR.
SUPFAMiSSF57027. SSF57027. 1 hit.
PROSITEiPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30
GCPRILMRCK QDSDCLAGCV CGPNGFCGSP
Length:30
Mass (Da):3,089
Last modified:June 1, 1994 - v2
Checksum:iBEE08D405B4AE17D
GO

Sequence databases

PIRiJQ1958.

Cross-referencesi

Sequence databases

PIRiJQ1958.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H9HX-ray1.50I1-30[»]
1H9IX-ray1.90I1-30[»]
1W7ZX-ray1.67A/B/C/D/E/F/G/H1-30[»]
2C4BX-ray1.30A/B16-29[»]
2ETINMR-A1-28[»]
2IT7NMR-A1-28[»]
2LETNMR-A1-28[»]
ProteinModelPortaliP12071.
SMRiP12071. Positions 2-29.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI07.003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP12071.

Family and domain databases

InterProiIPR000737. Prot_inh_squash.
IPR011052. Proteinase_amylase_inhib_dom.
[Graphical view]
PfamiPF00299. Squash. 1 hit.
[Graphical view]
PRINTSiPR00293. SQUASHINHBTR.
SUPFAMiSSF57027. SSF57027. 1 hit.
PROSITEiPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Protease inhibitors from Ecballium elaterium seeds."
    Favel A., Mattras H., Coletti-Previero M.-A., Zwilling R., Robinson E.A., Castro B.
    Int. J. Pept. Protein Res. 33:202-208(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Seed.
  2. "Use of restrained molecular dynamics in water to determine three-dimensional protein structure: prediction of the three-dimensional structure of Ecballium elaterium trypsin inhibitor II."
    Chiche L., Gaboriaud C., Heitz A., Mornon J.-P., Castro B., Kollman P.A.
    Proteins 6:405-417(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  3. "Folding of the squash trypsin inhibitor EETI II. Evidence of native and non-native local structural preferences in a linear analogue."
    Heitz A., Chiche L., Castro B.
    Eur. J. Biochem. 233:837-846(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF MUTANT WITH CYS REPLACED BY SER.

Entry informationi

Entry nameiITR2_ECBEL
AccessioniPrimary (citable) accession number: P12071
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: June 1, 1994
Last modified: December 9, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.