Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P12070

- XYLA_ARTS7

UniProt

P12070 - XYLA_ARTS7

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Xylose isomerase

Gene

xylA

Organism
Arthrobacter sp. (strain NRRL B3728)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-xylopyranose = D-xylulose.

Cofactori

Mg2+Note: Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 5411 Publication
Active sitei57 – 5711 Publication
Metal bindingi181 – 1811Magnesium 1
Metal bindingi217 – 2171Magnesium 1
Metal bindingi217 – 2171Magnesium 2
Metal bindingi220 – 2201Magnesium 2
Metal bindingi245 – 2451Magnesium 1
Metal bindingi255 – 2551Magnesium 2
Metal bindingi257 – 2571Magnesium 2
Metal bindingi293 – 2931Magnesium 1

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. xylose isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-xylose metabolic process Source: UniProtKB-HAMAP
  2. pentose-phosphate shunt Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Pentose shunt, Xylose metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

SABIO-RKP12070.

Names & Taxonomyi

Protein namesi
Recommended name:
Xylose isomerase (EC:5.3.1.5)
Gene namesi
Name:xylA
OrganismiArthrobacter sp. (strain NRRL B3728)
Taxonomic identifieri1669 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeMicrococcaceaeArthrobacter

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 395394Xylose isomerasePRO_0000195760Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
395
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Beta strandi11 – 144Combined sources
Helixi15 – 184Combined sources
Helixi36 – 4611Combined sources
Beta strandi50 – 545Combined sources
Helixi55 – 584Combined sources
Helixi65 – 8218Combined sources
Beta strandi88 – 903Combined sources
Beta strandi94 – 963Combined sources
Helixi97 – 993Combined sources
Helixi109 – 12820Combined sources
Beta strandi132 – 1365Combined sources
Beta strandi142 – 1454Combined sources
Helixi146 – 1483Combined sources
Helixi151 – 17222Combined sources
Beta strandi177 – 1804Combined sources
Beta strandi184 – 19310Combined sources
Helixi196 – 2038Combined sources
Helixi209 – 2113Combined sources
Beta strandi212 – 2143Combined sources
Helixi218 – 2225Combined sources
Turni223 – 2253Combined sources
Helixi228 – 23710Combined sources
Beta strandi251 – 2544Combined sources
Helixi265 – 27713Combined sources
Beta strandi290 – 2923Combined sources
Helixi302 – 32827Combined sources
Helixi330 – 3389Combined sources
Turni339 – 3424Combined sources
Helixi343 – 3453Combined sources
Helixi355 – 3606Combined sources
Turni362 – 3676Combined sources
Helixi370 – 3734Combined sources
Helixi380 – 39112Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DIDX-ray2.50A/B2-395[»]
1DIEX-ray2.50A/B2-395[»]
1XLAX-ray2.30A/B2-395[»]
1XLBX-ray2.50A/B2-395[»]
1XLCX-ray2.50A/B2-395[»]
1XLDX-ray2.50A/B2-395[»]
1XLEX-ray2.50A/B2-395[»]
1XLFX-ray2.50A/B2-395[»]
1XLGX-ray2.50A/B2-395[»]
1XLHX-ray2.50A/B2-395[»]
1XLIX-ray2.50A/B2-395[»]
1XLJX-ray2.50A/B2-395[»]
1XLKX-ray2.50A/B2-395[»]
1XLLX-ray2.50A/B2-395[»]
1XLMX-ray2.40A/B2-395[»]
4XIAX-ray2.30A/B3-395[»]
5XIAX-ray2.50A/B3-395[»]
ProteinModelPortaliP12070.
SMRiP12070. Positions 3-395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12070.

Family & Domainsi

Sequence similaritiesi

Belongs to the xylose isomerase family.Curated

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00455. Xylose_isom_A.
InterProiIPR013022. Xyl_isomerase-like_TIM-brl.
IPR013453. XylA_actinobac.
IPR001998. Xylose_isomerase.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PRINTSiPR00688. XYLOSISMRASE.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR02631. xylA_Arthro. 1 hit.
PROSITEiPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12070-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVQPTPADH FTFGLWTVGW TGADPFGVAT RKNLDPVEAV HKLAELGAYG
60 70 80 90 100
ITFHDNDLIP FDATEAEREK ILGDFNQALK DTGLKVPMVT TNLFSHPVFK
110 120 130 140 150
DGGFTSNDRS IRRFALAKVL HNIDLAAEMG AETFVMWGGR EGSEYDGSKD
160 170 180 190 200
LAAALDRMRE GVDTAAGYIK DKGYNLRIAL EPKPNEPRGD IFLPTVGHGL
210 220 230 240 250
AFIEQLEHGD IVGLNPETGH EQMAGLNFTH GIAQALWAEK LFHIDLNGQR
260 270 280 290 300
GIKYDQDLVF GHGDLTSAFF TVDLLENGFP NGGPKYTGPR HFDYKPSRTD
310 320 330 340 350
GYDGVWDSAK ANMSMYLLLK ERALAFRADP EVQEAMKTSG VFELGETTLN
360 370 380 390
AGESAADLMN DSASFAGFDA EAAAERNFAF IRLNQLAIEH LLGSR
Length:395
Mass (Da):43,292
Last modified:January 23, 2007 - v3
Checksum:iEA7A21FC47726369
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59466 Genomic DNA. Translation: CAA42073.1.
PIRiS16214.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59466 Genomic DNA. Translation: CAA42073.1 .
PIRi S16214.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DID X-ray 2.50 A/B 2-395 [» ]
1DIE X-ray 2.50 A/B 2-395 [» ]
1XLA X-ray 2.30 A/B 2-395 [» ]
1XLB X-ray 2.50 A/B 2-395 [» ]
1XLC X-ray 2.50 A/B 2-395 [» ]
1XLD X-ray 2.50 A/B 2-395 [» ]
1XLE X-ray 2.50 A/B 2-395 [» ]
1XLF X-ray 2.50 A/B 2-395 [» ]
1XLG X-ray 2.50 A/B 2-395 [» ]
1XLH X-ray 2.50 A/B 2-395 [» ]
1XLI X-ray 2.50 A/B 2-395 [» ]
1XLJ X-ray 2.50 A/B 2-395 [» ]
1XLK X-ray 2.50 A/B 2-395 [» ]
1XLL X-ray 2.50 A/B 2-395 [» ]
1XLM X-ray 2.40 A/B 2-395 [» ]
4XIA X-ray 2.30 A/B 3-395 [» ]
5XIA X-ray 2.50 A/B 3-395 [» ]
ProteinModelPortali P12070.
SMRi P12070. Positions 3-395.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P12070.

Miscellaneous databases

EvolutionaryTracei P12070.

Family and domain databases

Gene3Di 3.20.20.150. 1 hit.
HAMAPi MF_00455. Xylose_isom_A.
InterProi IPR013022. Xyl_isomerase-like_TIM-brl.
IPR013453. XylA_actinobac.
IPR001998. Xylose_isomerase.
[Graphical view ]
Pfami PF01261. AP_endonuc_2. 1 hit.
[Graphical view ]
PRINTSi PR00688. XYLOSISMRASE.
SUPFAMi SSF51658. SSF51658. 1 hit.
TIGRFAMsi TIGR02631. xylA_Arthro. 1 hit.
PROSITEi PS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "D-xylose (D-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Gene cloning, sequence and expression."
    Loviny-Anderton T., Shaw P.C., Shin M.K., Hartley B.S.
    Biochem. J. 277:263-271(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "D-xylose (D-glucose) isomerase from Arthrobacter strain N.R.R.L. B3728. Purification and properties."
    Smith C.A., Rangarajan M., Hartley B.S.
    Biochem. J. 277:255-261(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21, CHARACTERIZATION.
  3. "Mechanism for aldose-ketose interconversion by D-xylose isomerase involving ring opening followed by a 1,2-hydride shift."
    Collyer C.A., Henrick K., Blow D.M.
    J. Mol. Biol. 212:211-235(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVE SITES HIS-54 AND ASP-57.
  4. "Structures of D-xylose isomerase from Arthrobacter strain B3728 containing the inhibitors xylitol and D-sorbitol at 2.5-A and 2.3-A resolution, respectively."
    Henrick K., Collyer C.A., Blow D.M.
    J. Mol. Biol. 208:129-157(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  5. "Comparison of backbone structures of glucose isomerase from Streptomyces and Arthrobacter."
    Henrick K., Blow D.M., Carell H.L., Glusker J.P.
    Protein Eng. 1:467-469(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: COMPARISON OF X-RAY STRUCTURES.

Entry informationi

Entry nameiXYLA_ARTS7
AccessioniPrimary (citable) accession number: P12070
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3