P12068 (LYSC2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lysozyme C-2 EC=3.2.1.17 Alternative name(s): 1,4-beta-N-acetylmuramidase C
Organism	Sus scrofa (Pig) [Reference proteome]
Taxonomic identifier	9823 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Subcellular location	Secreted.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW digestion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.2
	Chain	19 – 146	128	Lysozyme C-2		PRO_0000018479
Sites
	Active site	53	1	By similarity
	Active site	69	1	By similarity
Amino acid modifications
	Disulfide bond	24 ↔ 144		By similarity
	Disulfide bond	48 ↔ 132		By similarity
	Disulfide bond	81 ↔ 97		By similarity
	Disulfide bond	93 ↔ 111		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12068 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: C2986F5CAAAF49F4	FASTA	146	16,484
	10 20 30 40 50 60 MKTLLVLALL LLSVSVQAKV YDRCEFARIL KKSGMDGYRG VSLANWVCLA KWESDFNTKA 70 80 90 100 110 120 INHNVGSTDY GIFQINSRYW CNDGKTPKAV NACHISCKVL LDDDLSQDIE CAKRVVRDPL 130 140 GVKAWVAWRA HCQNKDVSQY IRGCKL

References

[1]	"Evolution of stomach lysozyme: the pig lysozyme gene." Yu M., Irwin D.M. Mol. Phylogenet. Evol. 5:298-308(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Episodic evolution in the stomach lysozymes of ruminants." Jolles J., Jolles P., Bowman B.H., Prager E.M., Stewart C.-B., Wilson A.C. J. Mol. Evol. 28:528-535(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-146. Tissue: Stomach.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U28757 Genomic DNA. Translation: AAB16862.1.
RefSeq	NP_999557.2. NM_214392.2.
UniGene	Ssc.670.
3D structure databases
ProteinModelPortal	P12068.
SMR	P12068. Positions 19-144.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000000530; ENSSSCP00000000522; ENSSSCG00000000492.
GeneID	100157211.
KEGG	ssc:100157211.
Organism-specific databases
CTD	4069.
Phylogenomic databases
GeneTree	ENSGT00550000074398.
HOVERGEN	HBG052297.
KO	K13915.
OMA	CNALLQD.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. IPR023346. Lysozyme-like_dom. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC2_PIG

Accession

Primary (citable) accession number: P12068

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program