P12066 (LYSC_AXIAX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lysozyme C-1/C-2 EC=3.2.1.17 Alternative name(s): 1,4-beta-N-acetylmuramidase C
Organism	Axis axis (Axis deer) (Chital)
Taxonomic identifier	30531 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Cervidae › Cervinae › Axis

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides. The sequence of isozyme 1 is shown.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Biological process	Digestion
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW digestion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 129	129	Lysozyme C-1/C-2		PRO_0000208843
Sites
	Active site	35	1	By similarity
	Active site	53	1	By similarity
Amino acid modifications
	Disulfide bond	6 ↔ 127		By similarity
	Disulfide bond	30 ↔ 115		By similarity
	Disulfide bond	65 ↔ 81		By similarity
	Disulfide bond	77 ↔ 95		By similarity
Natural variations
	Natural variant	66	1	D → N in isozyme 2.
	Natural variant	88	1	N → D in isozyme 2.
	Natural variant	90	1	D → A in isozyme 2.
	Natural variant	94	1	T → A in isozyme 2.
	Natural variant	117	1	G → H in isozyme 2.

Sequences

	Sequence		Length	Mass (Da)
	P12066 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: A0AA48E69C2EB383	FASTA	129	14,446
	10 20 30 40 50 60 KVFERCELAR TLKELGLDGY KGVSLANWLC LTKWESSYNT KATNYNPGSE STDYGIFQIN 70 80 90 100 110 120 SKWWCDDGKT PNAVDGCHVA CSELMENNID KAVTCAKQIV REQGITAWVA WKSHCRGHDV SSYVEGCTL

References

[1]	"Episodic evolution in the stomach lysozymes of ruminants." Jolles J., Jolles P., Bowman B.H., Prager E.M., Stewart C.-B., Wilson A.C. J. Mol. Evol. 28:528-535(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Stomach.
[2]	"Concerted evolution of ruminant stomach lysozymes. Characterization of lysozyme cDNA clones from sheep and deer." Irwin D.M., Wilson A.C. J. Biol. Chem. 265:4944-4952(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOZYMES 1 AND 2).

Cross-references

Sequence databases
EMBL GenBank DDBJ	M32499 mRNA. Translation: AAA30344.1. M32500 mRNA. Translation: AAA30345.1.
PIR	G35558. H35558.
3D structure databases
ProteinModelPortal	P12066.
SMR	P12066. Positions 1-129.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG052297.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR019799. Glyco_hydro_22_CS. IPR000974. Glyco_hydro_22_lys. IPR023346. Lysozyme-like_dom. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. 1 hit. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC_AXIAX

Accession

Primary (citable) accession number: P12066

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program