P12064 (EDN2_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endothelin-2
Short name=ET-2

Alternative name(s):
Preproendothelin-2
Short name=PPET2

Gene names

Name:

EDN2

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the endothelin/sarafotoxin family.
Sequence caution	The sequence CAA40354.1 differs from that shown. Reason:

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Vasoactive Vasoconstrictor
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	artery smooth muscle contraction Inferred from electronic annotation. Source: Compara calcium-mediated signaling Inferred from electronic annotation. Source: Compara cytokine-mediated signaling pathway Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara inositol phosphate-mediated signaling Inferred from electronic annotation. Source: Compara macrophage activation Inferred from electronic annotation. Source: Compara macrophage chemotaxis Inferred from electronic annotation. Source: Compara neutrophil chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of heart rate Inferred from electronic annotation. Source: Compara positive regulation of leukocyte chemotaxis Inferred from electronic annotation. Source: Compara positive regulation of prostaglandin-endoperoxide synthase activity Inferred from electronic annotation. Source: Compara positive regulation of smooth muscle contraction Inferred from electronic annotation. Source: Compara prostaglandin biosynthetic process Inferred from electronic annotation. Source: Compara regulation of systemic arterial blood pressure by endothelin Inferred from electronic annotation. Source: Compara regulation of vasoconstriction Inferred from electronic annotation. Source: InterPro vein smooth muscle contraction Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 46	22			PRO_0000008084
	Peptide	49 – 69	21	Endothelin-2		PRO_0000008085
	Propeptide	70 – 178	109			PRO_0000008086
Regions
	Region	96 – 111	16	Endothelin-like
Sites
	Site	69 – 70	2	Cleavage; by KEL By similarity
Amino acid modifications
	Disulfide bond	49 ↔ 63		By similarity
	Disulfide bond	51 ↔ 59		By similarity
Experimental info
	Sequence conflict	19 – 20	2	VA → AH in CAA40354. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12064 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 5BE799BA3B9BB8AA	FASTA	178	19,723
	10 20 30 40 50 60 MPAPGVHHPN TASPFLKTVA AGKGQVAAAP EHPAPSARAR GSHLRPRRCS CSSWLDKECV 70 80 90 100 110 120 YFCHLDIIWV NTPGQTAPYG LGNPPRRRRR SLPKRCECSS GGDPACATFC HRRPWAEAVV 130 140 150 160 170 VPGSRSPADV FQAGRTWTSA GELLRQLRNI SAAKIRFPRR PQEAGRQLRP THPRRRKR

References

[1]	"Cloning of dog preproendothelin-2 cDNA and quantitative method of the gene expression by real-time PCR." Uchide T. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Canine endothelin-2: cDNA sequence for the mature peptide." Itoh Y., Kimura C., Onda H., Fujino M. Nucleic Acids Res. 17:5389-5389(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-74. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB089264 mRNA. Translation: BAC56713.1. X57038 Genomic DNA. Translation: CAA40354.1. Sequence problems.
PIR	S29563.
RefSeq	NP_001003002.1. NM_001003002.1.
UniGene	Cfa.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	9615.ENSCAFP00000003751.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCAFT00000045204; ENSCAFP00000038061; ENSCAFG00000002579.
GeneID	403508.
KEGG	cfa:403508.
Organism-specific databases
CTD	1907.
Phylogenomic databases
eggNOG	NOG43933.
GeneTree	ENSGT00530000063310.
HOVERGEN	HBG051441.
KO	K16367.
Family and domain databases
InterPro	IPR020475. Bibrotoxin/Sarafotoxin-D. IPR019764. Endothelin_toxin_CS. IPR001928. Endothln-like_toxin. IPR015658. ET-2. [Graphical view]
PANTHER	PTHR13874:SF4. PTHR13874:SF4. 1 hit.
Pfam	PF00322. Endothelin. 1 hit. [Graphical view]
PRINTS	PR00365. ENDOTHELIN.
SMART	SM00272. END. 2 hits. [Graphical view]
PROSITE	PS00270. ENDOTHELIN. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	20817022.

Entry information

Entry name

EDN2_CANFA

Accession

Primary (citable) accession number: P12064
Secondary accession number(s): Q867C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 19, 2004
Last modified:	April 3, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program