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Protein

Phosphoribosylformylglycinamidine synthase subunit PurS

Gene

purS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation2 Publications

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation1 Publication

Kineticsi

Kcat is 2.49 sec(-1) for FGAM synthase activity (at pH 7.2 and 37 degrees Celsius).

  1. KM=181 µM for ATP (at pH 7.2 and 37 degrees Celsius)1 Publication
  2. KM=507 µM for FGAR (at pH 7.2 and 37 degrees Celsius)1 Publication
  3. KM=1.3 mM for glutamine (at pH 7.2 and 37 degrees Celsius)1 Publication

    Pathwayi: IMP biosynthesis via de novo pathway

    This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS)
    2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
    This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU06460-MONOMER.
    RETL1328306-WGS:GSTH-2288-MONOMER.
    UniPathwayiUPA00074; UER00128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthase subunit PurSUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferase subunit IIIUniRule annotation
    Short name:
    FGAR amidotransferase IIIUniRule annotation
    Short name:
    FGAR-AT IIIUniRule annotation
    Phosphoribosylformylglycinamidine synthase subunit IIIUniRule annotation
    Gene namesi
    Name:purSUniRule annotation
    Synonyms:yexA
    Ordered Locus Names:BSU06460
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene show a purine auxotrophic phenotype and an accumulation of FGAR due to defective FGAM synthetase activity.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 8484Phosphoribosylformylglycinamidine synthase subunit PurSPRO_0000100395Add
    BLAST

    Proteomic databases

    PaxDbiP12049.

    Interactioni

    Subunit structurei

    Homodimer or homotetramer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.1 Publication

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100003648.

    Structurei

    Secondary structure

    1
    84
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 109Combined sources
    Helixi17 – 2812Combined sources
    Beta strandi34 – 4714Combined sources
    Helixi54 – 6411Combined sources
    Turni69 – 713Combined sources
    Beta strandi72 – 798Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1T4AX-ray2.00A/B1-84[»]
    1TWJX-ray2.50A/B/C/D1-84[»]
    ProteinModelPortaliP12049.
    SMRiP12049. Positions 1-80.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP12049.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PurS family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105VR9. Bacteria.
    COG1828. LUCA.
    HOGENOMiHOG000033801.
    InParanoidiP12049.
    KOiK01952.
    OMAiVIENYRF.
    PhylomeDBiP12049.

    Family and domain databases

    Gene3Di3.30.1280.10. 1 hit.
    HAMAPiMF_01926. PurS. 1 hit.
    InterProiIPR003850. PurS.
    [Graphical view]
    PfamiPF02700. PurS. 1 hit.
    [Graphical view]
    ProDomiPD010362. FGAM_PurS. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    TIGRFAMsiTIGR00302. TIGR00302. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P12049-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MYKVKVYVSL KESVLDPQGS AVQHALHSMT YNEVQDVRIG KYMELTIEKS
    60 70 80
    DRDLDVLVKE MCEKLLANTV IEDYRYEVEE VVAQ
    Length:84
    Mass (Da):9,755
    Last modified:October 1, 1989 - v1
    Checksum:iE7CD0BF1BB8874DD
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J02732 Genomic DNA. No translation available.
    AL009126 Genomic DNA. Translation: CAB12466.1.
    PIRiE29326.
    RefSeqiNP_388528.1. NC_000964.3.
    WP_003219409.1. NZ_JNCM01000032.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12466; CAB12466; BSU06460.
    GeneIDi936041.
    KEGGibsu:BSU06460.
    PATRICi18972934. VBIBacSub10457_0681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J02732 Genomic DNA. No translation available.
    AL009126 Genomic DNA. Translation: CAB12466.1.
    PIRiE29326.
    RefSeqiNP_388528.1. NC_000964.3.
    WP_003219409.1. NZ_JNCM01000032.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1T4AX-ray2.00A/B1-84[»]
    1TWJX-ray2.50A/B/C/D1-84[»]
    ProteinModelPortaliP12049.
    SMRiP12049. Positions 1-80.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100003648.

    Proteomic databases

    PaxDbiP12049.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB12466; CAB12466; BSU06460.
    GeneIDi936041.
    KEGGibsu:BSU06460.
    PATRICi18972934. VBIBacSub10457_0681.

    Phylogenomic databases

    eggNOGiENOG4105VR9. Bacteria.
    COG1828. LUCA.
    HOGENOMiHOG000033801.
    InParanoidiP12049.
    KOiK01952.
    OMAiVIENYRF.
    PhylomeDBiP12049.

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00128.
    BioCyciBSUB:BSU06460-MONOMER.
    RETL1328306-WGS:GSTH-2288-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiP12049.

    Family and domain databases

    Gene3Di3.30.1280.10. 1 hit.
    HAMAPiMF_01926. PurS. 1 hit.
    InterProiIPR003850. PurS.
    [Graphical view]
    PfamiPF02700. PurS. 1 hit.
    [Graphical view]
    ProDomiPD010362. FGAM_PurS. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    TIGRFAMsiTIGR00302. TIGR00302. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPURS_BACSU
    AccessioniPrimary (citable) accession number: P12049
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: September 7, 2016
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families
    5. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.