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Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Bifunctional purine biosynthesis protein PurH (purH)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase
Biological processPurine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU06520-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Synonyms:purHJ
Ordered Locus Names:BSU06520
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001920701 – 512Bifunctional purine biosynthesis protein PurHAdd BLAST512

Proteomic databases

PaxDbiP12048.
PRIDEiP12048.

Interactioni

Protein-protein interaction databases

IntActiP12048. 1 interactor.
MINTiP12048.
STRINGi224308.Bsubs1_010100003683.

Structurei

3D structure databases

ProteinModelPortaliP12048.
SMRiP12048.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 146MGS-likePROSITE-ProRule annotationAdd BLAST146

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105DC1. Bacteria.
COG0138. LUCA.
HOGENOMiHOG000230372.
InParanoidiP12048.
KOiK00602.
OMAiDLLFAWK.
PhylomeDBiP12048.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH. 1 hit.
InterProiView protein in InterPro
IPR024051. AICAR_Tfase_dup_dom_sf.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
IPR036914. MGS-like_dom_sf.
IPR002695. PurH-like.
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiView protein in Pfam
PF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiView protein in SMART
SM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.
PROSITEiView protein in PROSITE
PS51855. MGS. 1 hit.

Sequencei

Sequence statusi: Complete.

P12048-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKRALISV SDKTNLVPFV KELTELGVEV ISTGGTKKLL QENGVDVIGI
60 70 80 90 100
SEVTGFPEIM DGRLKTLHPN IHGGLLAVRG NEEHMAQINE HGIQPIDLVV
110 120 130 140 150
VNLYPFKETI SKEDVTYEEA IENIDIGGPG MLRAASKNHQ DVTVIVDPAD
160 170 180 190 200
YSPVLNQIKE EGSVSLQKKR ELAAKVFRHT AAYDALIADY LTNVVGEKEP
210 220 230 240 250
EQFTVTFEKK QSLRYGENPH QEATFYQTAL PVKGSIAQAE QLHGKELSYN
260 270 280 290 300
NIKDADAAVQ IVREFTEPAA VAVKHMNPCG VGTGKTIAEA FDRAFEADKT
310 320 330 340 350
SIFGGIIALN REVDKATAEA LHNIFLEIII APSFSQEALD VLTAKKNLRL
360 370 380 390 400
LTLDVSAAVQ KEKQLTSVQG GLLIQDLDMH GFDDAEISIP TKREPNEQEW
410 420 430 440 450
EDLKLAWKVV KHVKSNAIVL AKDNMTVGVG AGQMNRVGSA KIAIEQAGEK
460 470 480 490 500
AKGSALGSDA YFPMPDTVEE AAKAGVTAII QPGGSIRDED SIKKADEYGI
510
AMVFTGIRHF KH
Length:512
Mass (Da):55,753
Last modified:July 7, 2009 - v2
Checksum:i832CE152D9CEC358
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti351L → V in AAA22683 (PubMed:3036807).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02732 Genomic DNA. Translation: AAA22683.1.
AL009126 Genomic DNA. Translation: CAB12472.2.
AF011544 Genomic DNA. Translation: AAB72185.1.
PIRiA29183. DTBSPH.
RefSeqiNP_388534.2. NC_000964.3.
WP_003244516.1. NZ_JNCM01000032.1.

Genome annotation databases

EnsemblBacteriaiCAB12472; CAB12472; BSU06520.
GeneIDi936053.
KEGGibsu:BSU06520.
PATRICifig|224308.179.peg.708.

Similar proteinsi

Entry informationi

Entry nameiPUR9_BACSU
AccessioniPrimary (citable) accession number: P12048
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 7, 2009
Last modified: March 28, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome