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P12045 (PURK_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N5-carboxyaminoimidazole ribonucleotide synthase
Short name=N5-CAIR synthase
EC=6.3.4.18
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetase
Gene names
Name:purK
Ordered Locus Names:BSU06430
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) By similarity.

Catalytic activity

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the purK/purT family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 380380N5-carboxyaminoimidazole ribonucleotide synthase
PRO_0000074994

Regions

Domain112 – 298187ATP-grasp
Nucleotide binding183 – 1864ATP By similarity
Nucleotide binding268 – 2692ATP By similarity

Sites

Binding site1081ATP By similarity
Binding site1481ATP By similarity
Binding site1591ATP By similarity
Binding site1911ATP By similarity

Experimental info

Sequence conflict2271Missing in AAA22675. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P12045 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: 744FC91C68C07A83

FASTA38042,320
        10         20         30         40         50         60 
MSKQIIYPGA VIGIIGGGQL GKMMAVSAKQ MGYKVAVVDP VKDSPCGQVA DVEITAHYND 

        70         80         90        100        110        120 
REAIRKLAEI SDIITYEFEN IDYDALHWLK DHAYLPQGSE LLLITQNRET EKKAIQSAGC 

       130        140        150        160        170        180 
EVAPYSIVKT KNELKQAVQE LRLPAVLKTC RGGYDGKGQF VIKEEAQMEQ AAALLEHGTC 

       190        200        210        220        230        240 
ILESWVSFKM ELSVIVVRSV NGEISTFPTA ENIHHNNILF QSIVPARVEK GIQQKAADLA 

       250        260        270        280        290        300 
VKLADELNLV GPLAVEMFLT EDGELLVNEL APRPHNSGHY TLDLCETSQF EQHIRAVCGL 

       310        320        330        340        350        360 
PLGKTDLLKP GMMVNLLGDE VKLVEEDPEL LKEAKLYIYG KHEIKKGRKM GHITFMKQPE 

       370        380 
DEWIQEITNK WMNRDGGQAE

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis."
Ebbole D.J., Zalkin H.
J. Biol. Chem. 262:8274-8287(1987) [PubMed: 3036807] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 227.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J02732 Genomic DNA. Translation: AAA22675.1.
AL009126 Genomic DNA. Translation: CAB12463.2.
PIRDCBSPK. B29326.
RefSeqNP_388525.2. NC_000964.3.

3D structure databases

ProteinModelPortalP12045.
SMRP12045. Positions 6-378.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000000282; EBBACP00000000282; EBBACG00000000282.
GeneID936039.
GenomeReviewsGene locus BSU06430 in contig AL009126_GR.
KEGGbsu:BSU06430.
NMPDRfig|224308.1.peg.644.
PATRIC18972928. VBIBacSub10457_0678.

Organism-specific databases

GenoListBSU06430. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000001330.
HOGENOMHBG516369.
PhylomeDBP12045.
ProtClustDBPRK06019.

Enzyme and pathway databases

BioCycBSUB:BSU06430-MONOMER.

Family and domain databases

InterProIPR006115. 6PGDH_NADP-bd.
IPR005875. AIR_COase_ATPase-su.
IPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01589.
PfamPF02222. ATP-grasp. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMsTIGR01161. PurK. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePURK_BACSU
AccessionPrimary (citable) accession number: P12045
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 7, 2009
Last modified: January 25, 2012
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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