Reviewed, UniProtKB/Swiss-Prot P12044 (PUR6_BACSU)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase catalytic subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purE
Ordered Locus Names:	BSU06420

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This subunit can alone transform AIR to CAIR, but in association with purK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Phosphoribosylaminoimidazole carboxylase catalytic subunit		PRO_0000074969
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	14	1	Substrate By similarity
	Binding site	41	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12044-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 9257EDF0253B4666	FASTA	162	17,174
	10 20 30 40 50 60 MQPLVGIIMG STSDWETMKH ACDILDELNV PYEKKVVSAH RTPDFMFEYA ETARERGIKV 70 80 90 100 110 120 IIAGAGGAAH LPGMTAAKTT LPVIGVPVQS KALNGMDSLL SIVQMPGGVP VATTSIGKAG 130 140 150 160 AVNAGLLAAQ ILSAFDEDLA RKLDERRENT KQTVLESSDQ LV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis." Ebbole D.J., Zalkin H. J. Biol. Chem. 262:8274-8287(1987) [PubMed: 3036807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	Borriss R., Porwollik S., Schroeter R., Hahstedt C., Mueller C. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22. Strain: 168.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02732 Genomic DNA. Translation: AAA22674.1. AL009126 Genomic DNA. Translation: CAB12462.1. U51115 Genomic DNA. Translation: AAB62318.1.
PIR	DEBSPE. A29326.
RefSeq	NP_388524.1.
3D structure databases
HSSP	HSSP built from PDB template 1O4V based on UniProtKB Q9WYS7.
SMR	P12044. Positions 2-159.
ModBase	Search...
Genome annotation databases
GeneID	939481.
GenomeReviews	Gene locus BSU06420 in contig AL009126_GR.
KEGG	bsu:BSU06420.
NMPDR	fig|224308.1.peg.643.
Organism-specific databases
SubtiList	BG10700. purE. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P12044.
OMA	GVVMGSS.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0643-MON.
BRENDA	4.1.1.21. 150.
Family and domain databases
InterPro	IPR000031. AIR_COase_core. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
PANTHER	PTHR23046. AIR_carboxyl. 1 hit.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
ProDom	PD002193. AIR_carboxyl. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR6_BACSU

Accession

Primary (citable) accession number: P12044

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	November 3, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)