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P12043 (PUR5_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine cyclo-ligase

EC=6.3.3.1
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene names
Name:purM
Synonyms:ath
Ordered Locus Names:BSU06500
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesBacillaceaeBacillus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B

Subcellular location

Cytoplasm By similarity HAMAP MF_00741_B.

Sequence similarities

Belongs to the AIR synthase family.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine cyclo-ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B
PRO_0000148197

Experimental info

Sequence conflict203 – 2042EL → AW in AAA22681. Ref.1
Sequence conflict2191E → Q in AAA22681. Ref.1
Sequence conflict2221L → V in AAA22681. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P12043 [UniParc].

Last modified July 7, 2009. Version 2.
Checksum: 1A9524A69DFC3DA5

FASTA34637,052
        10         20         30         40         50         60 
MSEAYKNAGV DIEAGYEAVK RMKKHVERTK RLGVMGSLGG FGGMFDLSEL SYQKPVLISG 

        70         80         90        100        110        120 
TDGVGTKLKL AFSMDKHDTI GVDAVAMCVN DVLAQGAEPL FFLDYLAVGK ADPVKIEQIV 

       130        140        150        160        170        180 
QGVAEGCEQS GSALVGGETA EMPGLYTADE YDIAGFSVGV AEKDEIVTGE KIEEGHLLIG 

       190        200        210        220        230        240 
LSSSGLHSNG FSLVRKVLLD DAELDLDTTY EPFERPLGEE LLEPTRIYVK PVLAAVKSGK 

       250        260        270        280        290        300 
IDGMAHVTGG GFIENIPRML PEGLSAEIDH GSWPIPPIFS FLQEYGKLKE EDMFNVFNMG 

       310        320        330        340 
IGFVLAVKEE HLTDVIGTLE SHGEKAYLIG RVKKGEGVTF GGAALS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis."
Ebbole D.J., Zalkin H.
J. Biol. Chem. 262:8274-8287(1987) [PubMed: 3036807] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 203-204; 219 AND 222.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02732 Genomic DNA. Translation: AAA22681.1.
AL009126 Genomic DNA. Translation: CAB12470.2.
PIRAJBSCL. H29326.
RefSeqNP_388532.2. NC_000964.3.

3D structure databases

ProteinModelPortalP12043.
SMRP12043. Positions 13-340.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000001849; EBBACP00000001849; EBBACG00000001846.
GeneID936044.
GenomeReviewsGene locus BSU06500 in contig AL009126_GR.
KEGGbsu:BSU06500.
NMPDRfig|224308.1.peg.651.
PATRIC18972942. VBIBacSub10457_0685.

Organism-specific databases

GenoListBSU06500. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000002660.
HOGENOMHBG531222.
PhylomeDBP12043.
ProtClustDBPRK05385.

Enzyme and pathway databases

BioCycBSUB:BSU06500-MONOMER.

Family and domain databases

HAMAPMF_00741_B. AIRS_B.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
[Graphical view]
KOK01933.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 1 hit.
SSF55326. PurM_N-like. 1 hit.
TIGRFAMsTIGR00878. PurM. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR5_BACSU
AccessionPrimary (citable) accession number: P12043
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 7, 2009
Last modified: January 25, 2012
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families