P12043 (PUR5_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Synonyms:	ath
Ordered Locus Names:	BSU06500

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_0000148197
Experimental info
	Sequence conflict	203 – 204	2	EL → AW in AAA22681. Ref.1
	Sequence conflict	219	1	E → Q in AAA22681. Ref.1
	Sequence conflict	222	1	L → V in AAA22681. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12043 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 1A9524A69DFC3DA5	FASTA	346	37,052
	10 20 30 40 50 60 MSEAYKNAGV DIEAGYEAVK RMKKHVERTK RLGVMGSLGG FGGMFDLSEL SYQKPVLISG 70 80 90 100 110 120 TDGVGTKLKL AFSMDKHDTI GVDAVAMCVN DVLAQGAEPL FFLDYLAVGK ADPVKIEQIV 130 140 150 160 170 180 QGVAEGCEQS GSALVGGETA EMPGLYTADE YDIAGFSVGV AEKDEIVTGE KIEEGHLLIG 190 200 210 220 230 240 LSSSGLHSNG FSLVRKVLLD DAELDLDTTY EPFERPLGEE LLEPTRIYVK PVLAAVKSGK 250 260 270 280 290 300 IDGMAHVTGG GFIENIPRML PEGLSAEIDH GSWPIPPIFS FLQEYGKLKE EDMFNVFNMG 310 320 330 340 IGFVLAVKEE HLTDVIGTLE SHGEKAYLIG RVKKGEGVTF GGAALS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis." Ebbole D.J., Zalkin H. J. Biol. Chem. 262:8274-8287(1987) [PubMed: 3036807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 203-204; 219 AND 222.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02732 Genomic DNA. Translation: AAA22681.1. AL009126 Genomic DNA. Translation: CAB12470.2.
PIR	AJBSCL. H29326.
RefSeq	NP_388532.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P12043.
SMR	P12043. Positions 13-340.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000001849; EBBACP00000001849; EBBACG00000001846.
GeneID	936044.
GenomeReviews	Gene locus BSU06500 in contig AL009126_GR.
KEGG	bsu:BSU06500.
NMPDR	fig|224308.1.peg.651.
PATRIC	18972942. VBIBacSub10457_0685.
Organism-specific databases
GenoList	BSU06500. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002660.
HOGENOM	HBG531222.
PhylomeDB	P12043.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	BSUB:BSU06500-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_BACSU

Accession

Primary (citable) accession number: P12043

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 7, 2009
Last modified:	January 25, 2012
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program