P12043 (PUR5_BACSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 98.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Phosphoribosylformylglycinamidine cyclo-ligase EC=6.3.3.1 Alternative name(s): AIR synthase AIRS Phosphoribosyl-aminoimidazole synthetase | ||||||
| Gene names |
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| Organism | Bacillus subtilis (strain 168) [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 224308 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›  |
Protein attributes
| Sequence length | 346 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741_B |
| Pathway | Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741_B |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the AIR synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | 'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway |
| Cellular_component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 346 | 346 | Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741_B | PRO_0000148197 | |||||
Experimental info | |||||||||
| Sequence conflict | 203 – 204 | 2 | EL → AW in AAA22681. Ref.1 | ||||||
| Sequence conflict | 219 | 1 | E → Q in AAA22681. Ref.1 | ||||||
| Sequence conflict | 222 | 1 | L → V in AAA22681. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis." Ebbole D.J., Zalkin H. J. Biol. Chem. 262:8274-8287(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [3] | "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 203-204; 219 AND 222. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J02732 Genomic DNA. Translation: AAA22681.1. AL009126 Genomic DNA. Translation: CAB12470.2. |
| PIR | AJBSCL. H29326. |
| RefSeq | NP_388532.2. NC_000964.3. |
3D structure databases | |
| ProteinModelPortal | P12043. |
| SMR | P12043. Positions 13-340. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 224308.BSU06500. |
Proteomic databases | |
| PaxDb | P12043. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | CAB12470; CAB12470; BSU06500. |
| GeneID | 936044. |
| KEGG | bsu:BSU06500. |
| PATRIC | 18972942. VBIBacSub10457_0685. |
Organism-specific databases | |
| GenoList | BSU06500. [Micado] |
Phylogenomic databases | |
| eggNOG | COG0150. |
| HOGENOM | HOG000229091. |
| KO | K01933. |
| ProtClustDB | PRK05385. |
Enzyme and pathway databases | |
| BioCyc | BSUB:BSU06500-MONOMER. |
| UniPathway | UPA00074; UER00129. |
Family and domain databases | |
| HAMAP | MF_00741_B. AIRS_B. |
| InterPro | IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view] |
| Pfam | PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view] |
| SUPFAM | SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit. |
| TIGRFAMs | TIGR00878. purM. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | PUR5_BACSU | ||||||||
| Accession | Primary (citable) accession number: P12043 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |