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Protein

Phosphoribosylformylglycinamidine cyclo-ligase

Gene

purM

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU06500-MONOMER
UniPathwayiUPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:purM
Synonyms:ath
Ordered Locus Names:BSU06500
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001481971 – 346Phosphoribosylformylglycinamidine cyclo-ligaseAdd BLAST346

Proteomic databases

PaxDbiP12043
PRIDEiP12043

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100003673

Structurei

3D structure databases

ProteinModelPortaliP12043
SMRiP12043
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AIR synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105CXB Bacteria
COG0150 LUCA
HOGENOMiHOG000229091
InParanoidiP12043
KOiK01933
OMAiNHCVNDI
PhylomeDBiP12043

Family and domain databases

CDDicd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
InterProiView protein in InterPro
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
SUPFAMiSSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00878 purM, 1 hit

Sequencei

Sequence statusi: Complete.

P12043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAYKNAGV DIEAGYEAVK RMKKHVERTK RLGVMGSLGG FGGMFDLSEL
60 70 80 90 100
SYQKPVLISG TDGVGTKLKL AFSMDKHDTI GVDAVAMCVN DVLAQGAEPL
110 120 130 140 150
FFLDYLAVGK ADPVKIEQIV QGVAEGCEQS GSALVGGETA EMPGLYTADE
160 170 180 190 200
YDIAGFSVGV AEKDEIVTGE KIEEGHLLIG LSSSGLHSNG FSLVRKVLLD
210 220 230 240 250
DAELDLDTTY EPFERPLGEE LLEPTRIYVK PVLAAVKSGK IDGMAHVTGG
260 270 280 290 300
GFIENIPRML PEGLSAEIDH GSWPIPPIFS FLQEYGKLKE EDMFNVFNMG
310 320 330 340
IGFVLAVKEE HLTDVIGTLE SHGEKAYLIG RVKKGEGVTF GGAALS
Length:346
Mass (Da):37,052
Last modified:July 7, 2009 - v2
Checksum:i1A9524A69DFC3DA5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203 – 204EL → AW in AAA22681 (PubMed:3036807).Curated2
Sequence conflicti219E → Q in AAA22681 (PubMed:3036807).Curated1
Sequence conflicti222L → V in AAA22681 (PubMed:3036807).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02732 Genomic DNA Translation: AAA22681.1
AL009126 Genomic DNA Translation: CAB12470.2
PIRiH29326 AJBSCL
RefSeqiNP_388532.2, NC_000964.3
WP_003242485.1, NZ_JNCM01000032.1

Genome annotation databases

EnsemblBacteriaiCAB12470; CAB12470; BSU06500
GeneIDi936044
KEGGibsu:BSU06500
PATRICifig|224308.179.peg.706

Entry informationi

Entry nameiPUR5_BACSU
AccessioniPrimary (citable) accession number: P12043
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 7, 2009
Last modified: May 23, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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