P12042 (PURL_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	BSU06480

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 742	742	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_0000100440
Regions
	Nucleotide binding	111 – 122	12	ATP Potential
Experimental info
	Sequence conflict	513	1	F → L in AAA22679. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12042 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: 58F527FD16271557	FASTA	742	80,325
	10 20 30 40 50 60 MSLLLEPSKE QIKEEKLYQQ MGVSDDEFAL IESILGRLPN YTEIGIFSVM WSEHCSYKNS 70 80 90 100 110 120 KPILRKFPTS GERVLQGPGE GAGIVDIGDN QAVVFKIESH NHPSALEPYQ GAATGVGGII 130 140 150 160 170 180 RDVFSMGARP IAVLNSLRFG ELTSPRVKYL FEEVVAGIAG YGNCIGIPTV GGEVQFDSSY 190 200 210 220 230 240 EGNPLVNAMC VGLINHEDIK KGQAKGVGNT VMYVGAKTGR DGIHGATFAS EEMSDSSEEK 250 260 270 280 290 300 RSAVQVGDPF MEKLLLEACL EVIQCDALVG IQDMGAAGLT SSSAEMASKA GSGIEMNLDL 310 320 330 340 350 360 IPQRETGMTA YEMMLSESQE RMLLVIERGR EQEIIDIFDK YDLEAVSVGH VTDDKMLRLT 370 380 390 400 410 420 HKGEVVCELP VDALAEEAPV YHKPSQEPAY YREFLETDVP APQIEDANEM LKALLQQPTI 430 440 450 460 470 480 ASKEWVYDQY DYMVRTNTVV APGSDAGVLR IRGTKKALAM TTDCNARYLY LDPEVGGKIA 490 500 510 520 530 540 VAEAARNIIC SGAEPLAVTD NLNFGNPEKP EIFWQIEKAA DGISEACNVL STPVIGGNVS 550 560 570 580 590 600 LYNESNGTAI YPTPVIGMVG LIEDTAHITT QHFKQAGDLV YVIGETKPEF AGSELQKMTE 610 620 630 640 650 660 GRIYGKAPQI DLDVELSRQK ALLDAIKKGF VQSAHDVSEG GLGVAIAESV MTTENLGANV 670 680 690 700 710 720 TVEGEAALLF SESQSRFVVS VKKEHQAAFE ATVKDAVHIG EVTADGILAI QNQDGQQMIH 730 740 AQTKELERVW KGAIPCLLKS KA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis." Ebbole D.J., Zalkin H. J. Biol. Chem. 262:8274-8287(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 513.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02732 Genomic DNA. Translation: AAA22679.1. AL009126 Genomic DNA. Translation: CAB12468.2.
PIR	SYBS2G. G29326.
RefSeq	NP_388530.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P12042.
SMR	P12042. Positions 15-739.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU06480.
Proteomic databases
PaxDb	P12042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB12468; CAB12468; BSU06480.
GeneID	939233.
KEGG	bsu:BSU06480.
PATRIC	18972938. VBIBacSub10457_0683.
Organism-specific databases
GenoList	BSU06480. [Micado]
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	BSUB:BSU06480-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_BACSU

Accession

Primary (citable) accession number: P12042

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program