P12040 (PUR3_BACSU) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 92.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Phosphoribosylglycinamide formyltransferase EC=2.1.2.2 Alternative name(s): 5'-phosphoribosylglycinamide transformylase GAR transformylase Short name=GART | ||||
| Gene names |
| ||||
| Organism | Bacillus subtilis | ||||
| Taxonomic identifier | 1423 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 195 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide. |
| Pathway | |
| Sequence similarities | Belongs to the GART family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Molecular function | Transferase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | 'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro |
| Molecular function | methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylglycinamide formyltransferase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 195 | 195 | Phosphoribosylglycinamide formyltransferase | PRO_0000074942 | |||||
Regions | |||||||||
| Region | 12 – 14 | 3 | 5'-phosphoribosylglycinamide binding By similarity | ||||||
| Region | 90 – 93 | 4 | 10-formyltetrahydrofolate binding By similarity | ||||||
Sites | |||||||||
| Active site | 109 | 1 | Proton donor By similarity | ||||||
| Binding site | 65 | 1 | 10-formyltetrahydrofolate By similarity | ||||||
| Binding site | 107 | 1 | 10-formyltetrahydrofolate By similarity | ||||||
| Binding site | 145 | 1 | 10-formyltetrahydrofolate; via amide nitrogen By similarity | ||||||
| Binding site | 174 | 1 | 5'-phosphoribosylglycinamide By similarity | ||||||
| Site | 145 | 1 | Raises pKa of active site His By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 31 | 1 | A → R in AAA22682. Ref.1 | ||||||
| Sequence conflict | 49 | 1 | A → R in AAA22682. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis." Ebbole D.J., Zalkin H. J. Biol. Chem. 262:8274-8287(1987) [PubMed: 3036807] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.  Danchin A.Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168. |
| [3] | "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 31 AND 49. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J02732 Genomic DNA. Translation: AAA22682.1. AL009126 Genomic DNA. Translation: CAB12471.2. |
| PIR | XYBSGF. I29326. |
| RefSeq | NP_388533.2. NC_000964.3. |
3D structure databases | |
| ProteinModelPortal | P12040. |
| SMR | P12040. Positions 2-191. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBACT00000001573; EBBACP00000001573; EBBACG00000001571. |
| GeneID | 936045. |
| GenomeReviews | Gene locus BSU06510 in contig AL009126_GR. |
| KEGG | bsu:BSU06510. |
| NMPDR | fig|224308.1.peg.652. |
| PATRIC | 18972944. VBIBacSub10457_0686. |
Organism-specific databases | |
| GenoList | BSU06510. [Micado] |
Phylogenomic databases | |
| GeneTree | EBGT00050000001190. |
| HOGENOM | HBG649382. |
| PhylomeDB | P12040. |
| ProtClustDB | PRK05647. |
Enzyme and pathway databases | |
| BioCyc | BSUB:BSU06510-MONOMER. |
Family and domain databases | |
| InterPro | IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view] |
| Gene3D | G3DSA:3.40.50.170. Formyl_transf_N. 1 hit. |
| KO | K11175. |
| Pfam | PF00551. Formyl_trans_N. 1 hit. [Graphical view] |
| SUPFAM | SSF53328. formyl_transf. 1 hit. |
| TIGRFAMs | TIGR00639. PurN. 1 hit. |
| PROSITE | PS00373. GART. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUR3_BACSU | ||||||||
| Accession | Primary (citable) accession number: P12040 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Bacillus subtilis Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |