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Protein

Neurofilament heavy polypeptide

Gene

NEFH

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Neurofilament heavy polypeptide
Short name:
NF-H
Alternative name(s):
200 kDa neurofilament protein
Neurofilament triplet H protein
Gene namesi
Name:NEFH
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›142›142Neurofilament heavy polypeptidePRO_0000063802Add
BLAST

Post-translational modificationi

There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PeptideAtlasiP12037.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 7449Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000230977.

Family and domain databases

InterProiIPR001664. IF.
IPR033183. NF-H.
[Graphical view]
PANTHERiPTHR23214:SF1. PTHR23214:SF1. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P12037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGAVLRLGA ARGQLRLEQE HLLEDIAHVR QRLDDEARQR QEAEAAARAL
60 70 80 90 100
ARFAQEAEAA RVELQKKAQA LQEECGYLRR HHQEEAQAEA RDALKCDVTS
110 120 130 140
ALREIRAQLE GHAVQSTLQQ EEWFRVRLDR LSEAAKVNTD AM
Length:142
Mass (Da):16,204
Last modified:October 1, 1989 - v1
Checksum:iF19117D0F2DCFD8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei142 – 1421

Sequence databases

PIRiC22702.

Cross-referencesi

Sequence databases

PIRiC22702.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PeptideAtlasiP12037.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOGENOMiHOG000230977.

Family and domain databases

InterProiIPR001664. IF.
IPR033183. NF-H.
[Graphical view]
PANTHERiPTHR23214:SF1. PTHR23214:SF1. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Protein-chemical characterization of NF-H, the largest mammalian neurofilament component; intermediate filament-type sequences followed by a unique carboxy-terminal extension."
    Geisler N., Fischer S., Vandekerckhove J., van Damme J., Plessmann U., Weber K.
    EMBO J. 4:57-63(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.

Entry informationi

Entry nameiNFH_PIG
AccessioniPrimary (citable) accession number: P12037
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: July 6, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.