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Protein

Keratin, type II cytoskeletal 3

Gene

KRT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural molecule activity Source: UniProtKB

GO - Biological processi

  • epithelial cell differentiation Source: UniProtKB
  • intermediate filament cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172855-MONOMER.
ReactomeiR-HSA-6805567. Keratinization.
R-HSA-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type II cytoskeletal 3
Alternative name(s):
65 kDa cytokeratin
Cytokeratin-3
Short name:
CK-3
Keratin-3
Short name:
K3
Type-II keratin Kb3
Gene namesi
Name:KRT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:6440. KRT3.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • intermediate filament Source: UniProtKB
  • keratin filament Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Involvement in diseasei

Corneal dystrophy, Meesmann (MECD)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
See also OMIM:122100
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_031327503R → P in MECD. 1 PublicationCorresponds to variant rs60410063dbSNPEnsembl.1
Natural variantiVAR_003868509E → K in MECD. 1 PublicationCorresponds to variant rs57872071dbSNPEnsembl.1

Keywords - Diseasei

Corneal dystrophy, Disease mutation

Organism-specific databases

DisGeNETi3850.
MalaCardsiKRT3.
MIMi122100. phenotype.
OpenTargetsiENSG00000186442.
Orphaneti98954. Meesmann corneal dystrophy.
PharmGKBiPA30228.

Polymorphism and mutation databases

BioMutaiKRT3.
DMDMi313104225.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000637161 – 628Keratin, type II cytoskeletal 3Add BLAST628

Proteomic databases

PaxDbiP12035.
PeptideAtlasiP12035.
PRIDEiP12035.

PTM databases

iPTMnetiP12035.
PhosphoSitePlusiP12035.
SwissPalmiP12035.

Expressioni

Tissue specificityi

Cornea specific.

Gene expression databases

BgeeiENSG00000186442.
CleanExiHS_KRT3.
GenevisibleiP12035. HS.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

Protein-protein interaction databases

BioGridi110048. 16 interactors.
IntActiP12035. 28 interactors.
STRINGi9606.ENSP00000413479.

Structurei

3D structure databases

ProteinModelPortaliP12035.
SMRiP12035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 197HeadAdd BLAST197
Regioni198 – 509RodAdd BLAST312
Regioni198 – 233Coil 1AAdd BLAST36
Regioni234 – 254Linker 1Add BLAST21
Regioni255 – 346Coil 1BAdd BLAST92
Regioni347 – 370Linker 12Add BLAST24
Regioni371 – 509Coil 2Add BLAST139
Regioni510 – 628TailAdd BLAST119

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi10 – 169Gly-richAdd BLAST160
Compositional biasi536 – 612Gly-richAdd BLAST77

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IKBD. Eukaryota.
ENOG4111UGN. LUCA.
GeneTreeiENSGT00760000118796.
HOGENOMiHOG000230976.
HOVERGENiHBG013015.
InParanoidiP12035.
KOiK07605.
OMAiLFENHIN.
OrthoDBiEOG091G09KR.
PhylomeDBiP12035.
TreeFamiTF332742.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 3 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 2 hits.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12035-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRQASKTSG GGSQGFSGRS AVVSGSSRMS CVAHSGGAGG GAYGFRSGAG
60 70 80 90 100
GFGSRSLYNL GGNKSISISV AAGGSRAGGF GGGRSSCAFA GGYGGGFGSG
110 120 130 140 150
YGGGFGGGFG GGRGMGGGFG GAGGFGGAGG FGGAGGFGGP GGFGGSGGFG
160 170 180 190 200
GPGSLGSPGG FGPGGFPGGI QEVTINQSLL QPLNVEIDPQ IGQVKAQERE
210 220 230 240 250
QIKTLNNKFA SFIDKVRFLE QQNKVLETKW NLLQQQGTSS ISGTNNLEPL
260 270 280 290 300
FENHINYLRS YLDNILGERG RLDSELKNME DLVEDFKKKY EDEINKRTAA
310 320 330 340 350
ENEFVTLKKD VDSAYMNKVE LQAKVDALID EIDFLRTLYD AELSQMQSHI
360 370 380 390 400
SDTSVVLSMD NNRSLDLDSI IAEVRAQYED IAQRSKAEAE ALYQTKLGEL
410 420 430 440 450
QTTAGRHGDD LRNTKSEIIE LNRMIQRLRA EIEGVKKQNA NLQTAIAEAE
460 470 480 490 500
QHGEMALKDA NAKLQELQAA LQQAKDDLAR LLRDYQELMN VKLALDVEIA
510 520 530 540 550
TYRKLLEGEE YRMSGECPSA VSISVVSSST TSASAGGYGG GYGGGMGGGL
560 570 580 590 600
GGGFSAGGGS GSGFGRGGGG GIGGGFGGGS SGFSGGSGFG SISGARYGVS
610 620
GGGFSSASNR GGSIKFSQSS QSSQRYSR
Length:628
Mass (Da):64,417
Last modified:November 30, 2010 - v3
Checksum:i65FDB8CAA7F3C960
GO

Sequence cautioni

The sequence CAF31522 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162G → A in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti175I → T in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti184N → K in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti187I → T in CAA28991 (PubMed:2439698).Curated1
Sequence conflicti298T → Y in CAA28993 (PubMed:2439698).Curated1
Sequence conflicti397L → M in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti409D → G in CAF31522 (PubMed:15737194).Curated1
Sequence conflicti448E → Q in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti511Y → YS in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti562S → I in CAA28996 (PubMed:2439698).Curated1
Sequence conflicti580S → T in CAA28996 (PubMed:2439698).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06129744G → A.Corresponds to variant rs28721426dbSNPEnsembl.1
Natural variantiVAR_056023375R → G.1 PublicationCorresponds to variant rs3887954dbSNPEnsembl.1
Natural variantiVAR_031327503R → P in MECD. 1 PublicationCorresponds to variant rs60410063dbSNPEnsembl.1
Natural variantiVAR_003868509E → K in MECD. 1 PublicationCorresponds to variant rs57872071dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05418 Genomic DNA. Translation: CAA28991.1.
X05419 Genomic DNA. Translation: CAA28992.1.
X05420 Genomic DNA. Translation: CAA28993.1.
X05420 Genomic DNA. Translation: CAA28994.1.
X05420 Genomic DNA. Translation: CAA28995.1.
X05421 Genomic DNA. Translation: CAA28996.1.
AC107016 Genomic DNA. No translation available.
AJ628418 mRNA. Translation: CAF31522.1. Different initiation.
CCDSiCCDS44895.1.
PIRiA29666.
RefSeqiNP_476429.2. NM_057088.2.
UniGeneiHs.680652.

Genome annotation databases

EnsembliENST00000417996; ENSP00000413479; ENSG00000186442.
GeneIDi3850.
KEGGihsa:3850.
UCSCiuc001say.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Human Intermediate Filament Mutation Database
Wikipedia

Keratin-3 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05418 Genomic DNA. Translation: CAA28991.1.
X05419 Genomic DNA. Translation: CAA28992.1.
X05420 Genomic DNA. Translation: CAA28993.1.
X05420 Genomic DNA. Translation: CAA28994.1.
X05420 Genomic DNA. Translation: CAA28995.1.
X05421 Genomic DNA. Translation: CAA28996.1.
AC107016 Genomic DNA. No translation available.
AJ628418 mRNA. Translation: CAF31522.1. Different initiation.
CCDSiCCDS44895.1.
PIRiA29666.
RefSeqiNP_476429.2. NM_057088.2.
UniGeneiHs.680652.

3D structure databases

ProteinModelPortaliP12035.
SMRiP12035.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110048. 16 interactors.
IntActiP12035. 28 interactors.
STRINGi9606.ENSP00000413479.

PTM databases

iPTMnetiP12035.
PhosphoSitePlusiP12035.
SwissPalmiP12035.

Polymorphism and mutation databases

BioMutaiKRT3.
DMDMi313104225.

Proteomic databases

PaxDbiP12035.
PeptideAtlasiP12035.
PRIDEiP12035.

Protocols and materials databases

DNASUi3850.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000417996; ENSP00000413479; ENSG00000186442.
GeneIDi3850.
KEGGihsa:3850.
UCSCiuc001say.4. human.

Organism-specific databases

CTDi3850.
DisGeNETi3850.
GeneCardsiKRT3.
H-InvDBHIX0036742.
HGNCiHGNC:6440. KRT3.
MalaCardsiKRT3.
MIMi122100. phenotype.
148043. gene.
neXtProtiNX_P12035.
OpenTargetsiENSG00000186442.
Orphaneti98954. Meesmann corneal dystrophy.
PharmGKBiPA30228.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKBD. Eukaryota.
ENOG4111UGN. LUCA.
GeneTreeiENSGT00760000118796.
HOGENOMiHOG000230976.
HOVERGENiHBG013015.
InParanoidiP12035.
KOiK07605.
OMAiLFENHIN.
OrthoDBiEOG091G09KR.
PhylomeDBiP12035.
TreeFamiTF332742.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000172855-MONOMER.
ReactomeiR-HSA-6805567. Keratinization.
R-HSA-6809371. Formation of the cornified envelope.

Miscellaneous databases

GeneWikiiKeratin_3.
GenomeRNAii3850.
PROiP12035.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186442.
CleanExiHS_KRT3.
GenevisibleiP12035. HS.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR032444. Keratin_2_head.
IPR003054. Keratin_II.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 3 hits.
PfamiPF00038. Filament. 1 hit.
PF16208. Keratin_2_head. 2 hits.
[Graphical view]
PRINTSiPR01276. TYPE2KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK2C3_HUMAN
AccessioniPrimary (citable) accession number: P12035
Secondary accession number(s): A6NIS2, Q701L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 30, 2010
Last modified: November 30, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.