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P12035

- K2C3_HUMAN

UniProt

P12035 - K2C3_HUMAN

Protein

Keratin, type II cytoskeletal 3

Gene

KRT3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 3 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    GO - Molecular functioni

    1. structural molecule activity Source: UniProtKB

    GO - Biological processi

    1. epithelial cell differentiation Source: UniProtKB
    2. intermediate filament cytoskeleton organization Source: UniProtKB

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Keratin, type II cytoskeletal 3
    Alternative name(s):
    65 kDa cytokeratin
    Cytokeratin-3
    Short name:
    CK-3
    Keratin-3
    Short name:
    K3
    Type-II keratin Kb3
    Gene namesi
    Name:KRT3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:6440. KRT3.

    Subcellular locationi

    GO - Cellular componenti

    1. intermediate filament Source: UniProtKB
    2. keratin filament Source: UniProtKB

    Keywords - Cellular componenti

    Intermediate filament, Keratin

    Pathology & Biotechi

    Involvement in diseasei

    Corneal dystrophy, Meesmann (MECD) [MIM:122100]: An autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.2 Publications
    Note: The disease is caused by mutations affecting the gene represented in this entry.
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti503 – 5031R → P in MECD. 1 Publication
    Corresponds to variant rs60410063 [ dbSNP | Ensembl ].
    VAR_031327
    Natural varianti509 – 5091E → K in MECD. 1 Publication
    Corresponds to variant rs57872071 [ dbSNP | Ensembl ].
    VAR_003868

    Keywords - Diseasei

    Corneal dystrophy, Disease mutation

    Organism-specific databases

    MIMi122100. phenotype.
    Orphaneti98954. Meesmann corneal dystrophy.
    PharmGKBiPA30228.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 628628Keratin, type II cytoskeletal 3PRO_0000063716Add
    BLAST

    Proteomic databases

    MaxQBiP12035.
    PaxDbiP12035.
    PRIDEiP12035.

    PTM databases

    PhosphoSiteiP12035.

    Expressioni

    Tissue specificityi

    Cornea specific.

    Gene expression databases

    ArrayExpressiP12035.
    BgeeiP12035.
    CleanExiHS_KRT3.
    GenevestigatoriP12035.

    Interactioni

    Subunit structurei

    Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

    Protein-protein interaction databases

    BioGridi110048. 14 interactions.
    IntActiP12035. 3 interactions.
    STRINGi9606.ENSP00000413479.

    Structurei

    3D structure databases

    ProteinModelPortaliP12035.
    SMRiP12035. Positions 198-339, 366-508.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 197197HeadAdd
    BLAST
    Regioni198 – 509312RodAdd
    BLAST
    Regioni198 – 23336Coil 1AAdd
    BLAST
    Regioni234 – 25421Linker 1Add
    BLAST
    Regioni255 – 34692Coil 1BAdd
    BLAST
    Regioni347 – 37024Linker 12Add
    BLAST
    Regioni371 – 509139Coil 2Add
    BLAST
    Regioni510 – 628119TailAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi10 – 169160Gly-richAdd
    BLAST
    Compositional biasi536 – 61277Gly-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the intermediate filament family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG146968.
    HOGENOMiHOG000230976.
    HOVERGENiHBG013015.
    InParanoidiP12035.
    KOiK07605.
    OrthoDBiEOG7FV3Q8.
    PhylomeDBiP12035.
    TreeFamiTF332742.

    Family and domain databases

    InterProiIPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR003054. Keratin_II.
    [Graphical view]
    PANTHERiPTHR23239. PTHR23239. 1 hit.
    PfamiPF00038. Filament. 1 hit.
    [Graphical view]
    PRINTSiPR01276. TYPE2KERATIN.
    PROSITEiPS00226. IF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12035-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRQASKTSG GGSQGFSGRS AVVSGSSRMS CVAHSGGAGG GAYGFRSGAG    50
    GFGSRSLYNL GGNKSISISV AAGGSRAGGF GGGRSSCAFA GGYGGGFGSG 100
    YGGGFGGGFG GGRGMGGGFG GAGGFGGAGG FGGAGGFGGP GGFGGSGGFG 150
    GPGSLGSPGG FGPGGFPGGI QEVTINQSLL QPLNVEIDPQ IGQVKAQERE 200
    QIKTLNNKFA SFIDKVRFLE QQNKVLETKW NLLQQQGTSS ISGTNNLEPL 250
    FENHINYLRS YLDNILGERG RLDSELKNME DLVEDFKKKY EDEINKRTAA 300
    ENEFVTLKKD VDSAYMNKVE LQAKVDALID EIDFLRTLYD AELSQMQSHI 350
    SDTSVVLSMD NNRSLDLDSI IAEVRAQYED IAQRSKAEAE ALYQTKLGEL 400
    QTTAGRHGDD LRNTKSEIIE LNRMIQRLRA EIEGVKKQNA NLQTAIAEAE 450
    QHGEMALKDA NAKLQELQAA LQQAKDDLAR LLRDYQELMN VKLALDVEIA 500
    TYRKLLEGEE YRMSGECPSA VSISVVSSST TSASAGGYGG GYGGGMGGGL 550
    GGGFSAGGGS GSGFGRGGGG GIGGGFGGGS SGFSGGSGFG SISGARYGVS 600
    GGGFSSASNR GGSIKFSQSS QSSQRYSR 628
    Length:628
    Mass (Da):64,417
    Last modified:November 30, 2010 - v3
    Checksum:i65FDB8CAA7F3C960
    GO

    Sequence cautioni

    The sequence CAF31522.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti162 – 1621G → A in CAA28991. (PubMed:2439698)Curated
    Sequence conflicti175 – 1751I → T in CAA28991. (PubMed:2439698)Curated
    Sequence conflicti184 – 1841N → K in CAA28991. (PubMed:2439698)Curated
    Sequence conflicti187 – 1871I → T in CAA28991. (PubMed:2439698)Curated
    Sequence conflicti298 – 2981T → Y in CAA28993. (PubMed:2439698)Curated
    Sequence conflicti397 – 3971L → M in CAA28996. (PubMed:2439698)Curated
    Sequence conflicti409 – 4091D → G in CAF31522. (PubMed:15737194)Curated
    Sequence conflicti448 – 4481E → Q in CAA28996. (PubMed:2439698)Curated
    Sequence conflicti511 – 5111Y → YS in CAA28996. (PubMed:2439698)Curated
    Sequence conflicti562 – 5621S → I in CAA28996. (PubMed:2439698)Curated
    Sequence conflicti580 – 5801S → T in CAA28996. (PubMed:2439698)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti44 – 441G → A.
    Corresponds to variant rs28721426 [ dbSNP | Ensembl ].
    VAR_061297
    Natural varianti375 – 3751R → G.1 Publication
    Corresponds to variant rs3887954 [ dbSNP | Ensembl ].
    VAR_056023
    Natural varianti503 – 5031R → P in MECD. 1 Publication
    Corresponds to variant rs60410063 [ dbSNP | Ensembl ].
    VAR_031327
    Natural varianti509 – 5091E → K in MECD. 1 Publication
    Corresponds to variant rs57872071 [ dbSNP | Ensembl ].
    VAR_003868

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05418 Genomic DNA. Translation: CAA28991.1.
    X05419 Genomic DNA. Translation: CAA28992.1.
    X05420 Genomic DNA. Translation: CAA28993.1.
    X05420 Genomic DNA. Translation: CAA28994.1.
    X05420 Genomic DNA. Translation: CAA28995.1.
    X05421 Genomic DNA. Translation: CAA28996.1.
    AC107016 Genomic DNA. No translation available.
    AJ628418 mRNA. Translation: CAF31522.1. Different initiation.
    CCDSiCCDS44895.1.
    PIRiA29666.
    RefSeqiNP_476429.2. NM_057088.2.
    UniGeneiHs.680652.

    Genome annotation databases

    EnsembliENST00000417996; ENSP00000413479; ENSG00000186442.
    GeneIDi3850.
    KEGGihsa:3850.
    UCSCiuc001say.3. human.

    Polymorphism databases

    DMDMi313104225.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Human Intermediate Filament Mutation Database
    Wikipedia

    Keratin-3 entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05418 Genomic DNA. Translation: CAA28991.1 .
    X05419 Genomic DNA. Translation: CAA28992.1 .
    X05420 Genomic DNA. Translation: CAA28993.1 .
    X05420 Genomic DNA. Translation: CAA28994.1 .
    X05420 Genomic DNA. Translation: CAA28995.1 .
    X05421 Genomic DNA. Translation: CAA28996.1 .
    AC107016 Genomic DNA. No translation available.
    AJ628418 mRNA. Translation: CAF31522.1 . Different initiation.
    CCDSi CCDS44895.1.
    PIRi A29666.
    RefSeqi NP_476429.2. NM_057088.2.
    UniGenei Hs.680652.

    3D structure databases

    ProteinModelPortali P12035.
    SMRi P12035. Positions 198-339, 366-508.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110048. 14 interactions.
    IntActi P12035. 3 interactions.
    STRINGi 9606.ENSP00000413479.

    PTM databases

    PhosphoSitei P12035.

    Polymorphism databases

    DMDMi 313104225.

    Proteomic databases

    MaxQBi P12035.
    PaxDbi P12035.
    PRIDEi P12035.

    Protocols and materials databases

    DNASUi 3850.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000417996 ; ENSP00000413479 ; ENSG00000186442 .
    GeneIDi 3850.
    KEGGi hsa:3850.
    UCSCi uc001say.3. human.

    Organism-specific databases

    CTDi 3850.
    GeneCardsi GC12M053183.
    H-InvDB HIX0036742.
    HGNCi HGNC:6440. KRT3.
    MIMi 122100. phenotype.
    148043. gene.
    neXtProti NX_P12035.
    Orphaneti 98954. Meesmann corneal dystrophy.
    PharmGKBi PA30228.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG146968.
    HOGENOMi HOG000230976.
    HOVERGENi HBG013015.
    InParanoidi P12035.
    KOi K07605.
    OrthoDBi EOG7FV3Q8.
    PhylomeDBi P12035.
    TreeFami TF332742.

    Miscellaneous databases

    GeneWikii Keratin_3.
    GenomeRNAii 3850.
    NextBioi 15149.
    PROi P12035.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P12035.
    Bgeei P12035.
    CleanExi HS_KRT3.
    Genevestigatori P12035.

    Family and domain databases

    InterProi IPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR003054. Keratin_II.
    [Graphical view ]
    PANTHERi PTHR23239. PTHR23239. 1 hit.
    Pfami PF00038. Filament. 1 hit.
    [Graphical view ]
    PRINTSi PR01276. TYPE2KERATIN.
    PROSITEi PS00226. IF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of keratin genes: different protein domains evolve by different pathways."
      Klinge E.M., Sylvestre Y.R., Freedberg I.M., Blumenberg M.
      J. Mol. Evol. 24:319-329(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-375.
    2. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Characterization of new members of the human type II keratin gene family and a general evaluation of the keratin gene domain on chromosome 12q13.13."
      Rogers M.A., Edler L., Winter H., Langbein L., Beckmann I., Schweizer J.
      J. Invest. Dermatol. 124:536-544(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 16-628.
      Tissue: Eye.
    4. Cited for: VARIANT MECD LYS-509.
    5. "Novel mutations in the helix termination motif of keratin 3 and keratin 12 in 2 Taiwanese families with Meesmann corneal dystrophy."
      Chen Y.T., Tseng S.H., Chao S.C.
      Cornea 24:928-932(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANT MECD PRO-503.

    Entry informationi

    Entry nameiK2C3_HUMAN
    AccessioniPrimary (citable) accession number: P12035
    Secondary accession number(s): A6NIS2, Q701L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 142 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3