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P12032 (TIMP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metalloproteinase inhibitor 1
Alternative name(s):
Collagenase inhibitor 16C8 fibroblast
Erythroid-potentiating activity
Short name=EPA
TPA-S1
TPA-induced protein
Tissue inhibitor of metalloproteinases 1
Short name=TIMP-1
Gene names
Name:Timp1
Synonyms:Timp, Timp-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length205 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors.

Subcellular location

Secreted.

Tissue specificity

Found in fetal and adult tissues. Highest levels are found in bone. Also found in lung, ovary and uterus.

Developmental stage

Present in unfertilized eggs and at the zygote and cleavage stages. Levels increase at the blastocyst stage and with endoderm differentiation. Ref.5

Induction

Regulated by tumor promoters and mitogens through protein kinase C. Also induced by viruses.

Post-translational modification

The activity of TIMP1 is dependent on the presence of disulfide bonds.

Sequence similarities

Belongs to the protease inhibitor I35 (TIMP) family. [View classification]

Contains 1 NTR domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 205181Metalloproteinase inhibitor 1
PRO_0000034325

Regions

Domain25 – 148124NTR
Region25 – 295Involved in metalloproteinase-binding By similarity
Region91 – 922Involved in metalloproteinase-binding By similarity

Sites

Metal binding251Zinc; via amino nitrogen and carbonyl oxygen; shared with metalloproteinase partner By similarity

Amino acid modifications

Glycosylation541N-linked (GlcNAc...) Potential
Glycosylation1021N-linked (GlcNAc...) Potential
Disulfide bond25 ↔ 94 By similarity
Disulfide bond27 ↔ 123 By similarity
Disulfide bond37 ↔ 148 By similarity
Disulfide bond151 ↔ 197 By similarity
Disulfide bond156 ↔ 161 By similarity
Disulfide bond169 ↔ 189 By similarity

Experimental info

Sequence conflict521E → R in AAB42179. Ref.1
Sequence conflict661M → MM in AAB42179. Ref.1
Sequence conflict117 – 1182NL → KF in AAB42179. Ref.1
Sequence conflict1211S → N in AAB42179. Ref.1
Sequence conflict1391A → V Ref.1
Sequence conflict1431T → KN Ref.1
Sequence conflict1941P → L Ref.1
Sequence conflict1941P → L Ref.6

Sequences

Sequence LengthMass (Da)Tools
P12032 [UniParc].

Last modified May 1, 1991. Version 2.
Checksum: FACA952D49A50FD7

FASTA20522,628
        10         20         30         40         50         60 
MMAPFASLAS GILLLLSLIA SSKACSCAPP HPQTAFCNSD LVIRAKFMGS PEINETTLYQ 

        70         80         90        100        110        120 
RYKIKMTKML KGFKAVGNAA DIRYAYTPVM ESLCGYAHKS QNRSEEFLIT GRLRNGNLHI 

       130        140        150        160        170        180 
SACSFLVPWR TLSPAQQRAF SKTYSAGCGV CTVFPCLSIP CKLESDTHCL WTDQVLVGSE 

       190        200 
DYQSRHFACL PRNPGLCTWR SLGAR 

« Hide

References

« Hide 'large scale' references
[1]"Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mouse."
Gewert D.R., Coulombe B., Castelino M., Skup D., Williams B.R.G.
EMBO J. 6:651-657(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A growth-responsive gene (16C8) in normal mouse fibroblasts homologous to a human collagenase inhibitor with erythroid-potentiating activity: evidence for inducible and constitutive transcripts."
Edwards D.R., Waterhouse P., Holman M.L., Denhardt D.T.
Nucleic Acids Res. 14:8863-8878(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Fibroblast.
[3]"Molecular cloning of gene sequences regulated by tumor promoters and mitogens through protein kinase C."
Johnson M.D., Housey G.M., Kirschmeier P.T., Weinstein I.B.
Mol. Cell. Biol. 7:2821-2829(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland and Osteoblast.
[5]"Genes for extracellular-matrix-degrading metalloproteinases and their inhibitor, TIMP, are expressed during early mammalian development."
Brenner C.A., Adler R.R., Rappolee D.A., Pedersen R.A., Werb Z.
Genes Dev. 3:848-859(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE, DEVELOPMENTAL STAGE.
Tissue: Embryo.
[6]"Molecular cloning of partial cDNA copies of two distinct mouse IFN-beta mRNAs."
Skup D., Windass J.D., Sor F.S., George H., Williams B.R., Fukuhara H., de Maeyer-Guignard J., de Maeyer E.
Nucleic Acids Res. 10:3069-3084(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 168-205.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M28312 expand/collapse EMBL AC list , M28308, M28309, M28310, M28311 Genomic DNA. Translation: AAB42179.1.
X04684 mRNA. Translation: CAA28387.1.
M17243 mRNA. Translation: AAA40471.1.
BC008107 mRNA. Translation: AAH08107.1.
BC034260 mRNA. Translation: AAH34260.1.
BC051260 mRNA. Translation: AAH51260.1.
V00755 mRNA. Translation: CAA24132.1.
PIRA26106. A26917.
RefSeqNP_001037849.1. NM_001044384.1.
NP_035723.2. NM_011593.2.
UniGeneMm.8245.

3D structure databases

ProteinModelPortalP12032.
SMRP12032. Positions 25-202.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996416.
STRING10090.ENSMUSP00000110999.

Protein family/group databases

MEROPSI35.001.

PTM databases

PhosphoSiteP12032.

Proteomic databases

PaxDbP12032.
PRIDEP12032.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009530; ENSMUSP00000009530; ENSMUSG00000001131.
ENSMUST00000115342; ENSMUSP00000110999; ENSMUSG00000001131.
GeneID21857.
KEGGmmu:21857.
UCSCuc009sty.1. mouse.

Organism-specific databases

CTD7076.
MGIMGI:98752. Timp1.

Phylogenomic databases

eggNOGNOG259409.
GeneTreeENSGT00390000004555.
HOGENOMHOG000285981.
HOVERGENHBG068749.
InParanoidP12032.
KOK16451.
OMASSIPCKL.
OrthoDBEOG79GT74.
PhylomeDBP12032.
TreeFamTF317409.

Gene expression databases

BgeeP12032.
CleanExMM_TIMP1.
GenevestigatorP12032.

Family and domain databases

Gene3D3.90.370.10. 1 hit.
InterProIPR001134. Netrin_domain.
IPR001820. Prot_inh_TIMP.
IPR008993. TIMP-like_OB-fold.
IPR015611. TIMP1.
IPR027465. TIMP_C_dom.
[Graphical view]
PANTHERPTHR11844. PTHR11844. 1 hit.
PTHR11844:SF5. PTHR11844:SF5. 1 hit.
PfamPF00965. TIMP. 1 hit.
[Graphical view]
SMARTSM00206. NTR. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
PROSITEPS50189. NTR. 1 hit.
PS00288. TIMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301350.
PROP12032.
SOURCESearch...

Entry information

Entry nameTIMP1_MOUSE
AccessionPrimary (citable) accession number: P12032
Secondary accession number(s): P20064, Q61720
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 1991
Last modified: April 16, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot