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Protein

Cysteine-rich secretory protein 1

Gene

Crisp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This protein is supposed to help spermatozoa undergo functional maturation while they move from the testis to the ductus deferens.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine-rich secretory protein 1
Alternative name(s):
32 kDa epididymal protein
Acidic epididymal glycoprotein
Short name:
Protein D
Short name:
Protein E
Protein IV
Sialoprotein
Sperm-coating glycoprotein
Short name:
SCP
Gene namesi
Name:Crisp1
Synonyms:Aeg
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi619846. Crisp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 246227Cysteine-rich secretory protein 1PRO_0000006263Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...)Sequence analysis
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence analysis
Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi192 ↔ 199PROSITE-ProRule annotation
Disulfide bondi195 ↔ 204PROSITE-ProRule annotation
Disulfide bondi208 ↔ 241PROSITE-ProRule annotation
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence analysis
Disulfide bondi217 ↔ 235PROSITE-ProRule annotation
Disulfide bondi226 ↔ 239PROSITE-ProRule annotation

Post-translational modificationi

Two major variant protein D and E differ from each other by their carbohydrate side chains.
The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP12020.

Expressioni

Tissue specificityi

Secreted by the epididymal epithelium and then becomes associated with the sperm surface.

Inductioni

By androgens.

Gene expression databases

GenevisibleiP12020. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018162.

Structurei

3D structure databases

ProteinModelPortaliP12020.
SMRiP12020. Positions 192-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 172127SCPAdd
BLAST
Domaini208 – 24134ShKTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CRISP family.Curated
Contains 1 SCP domain.Curated
Contains 1 ShKT domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3017. Eukaryota.
COG2340. LUCA.
GeneTreeiENSGT00760000119004.
HOGENOMiHOG000236338.
HOVERGENiHBG004184.
InParanoidiP12020.
KOiK19919.
OMAiWINEPVY.
OrthoDBiEOG7F7WB4.
PhylomeDBiP12020.
TreeFamiTF316148.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12020-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALMLVLLFL AAVLPPSLLQ DTTDEWDRDL ENLSTTKLSV QEEIINKHNQ
60 70 80 90 100
LRRTVSPSGS DLLRVEWDHD AYVNAQKWAN RCIYNHSPLQ HRTTTLKCGE
110 120 130 140 150
NLFMANYPAS WSSVIQDWYD ESLDFVFGFG PKKVGVKVGH YTQVVWNSTF
160 170 180 190 200
LVACGVAECP DQPLKYFYVC HYCPGGNYVG RLYSPYTEGE PCDSCPGNCE
210 220 230 240
DGLCTNSCEY EDNYSNCGDL KKMVSCDDPL LKEGCRASCF CEDKIH
Length:246
Mass (Da):27,847
Last modified:October 1, 1989 - v1
Checksum:i585207C7CF7CF9D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04643 mRNA. Translation: CAA28304.1.
M31173 mRNA. Translation: AAB59716.1.
PIRiA40918. A24609.
RefSeqiNP_074050.1. NM_022859.1.
UniGeneiRn.10102.

Genome annotation databases

EnsembliENSRNOT00000018163; ENSRNOP00000018162; ENSRNOG00000013496.
GeneIDi64827.
KEGGirno:64827.
UCSCiRGD:619846. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04643 mRNA. Translation: CAA28304.1.
M31173 mRNA. Translation: AAB59716.1.
PIRiA40918. A24609.
RefSeqiNP_074050.1. NM_022859.1.
UniGeneiRn.10102.

3D structure databases

ProteinModelPortaliP12020.
SMRiP12020. Positions 192-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018162.

Proteomic databases

PaxDbiP12020.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018163; ENSRNOP00000018162; ENSRNOG00000013496.
GeneIDi64827.
KEGGirno:64827.
UCSCiRGD:619846. rat.

Organism-specific databases

CTDi167.
RGDi619846. Crisp1.

Phylogenomic databases

eggNOGiKOG3017. Eukaryota.
COG2340. LUCA.
GeneTreeiENSGT00760000119004.
HOGENOMiHOG000236338.
HOVERGENiHBG004184.
InParanoidiP12020.
KOiK19919.
OMAiWINEPVY.
OrthoDBiEOG7F7WB4.
PhylomeDBiP12020.
TreeFamiTF316148.

Miscellaneous databases

NextBioi613738.
PROiP12020.

Gene expression databases

GenevisibleiP12020. RN.

Family and domain databases

Gene3Di3.40.33.10. 1 hit.
InterProiIPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR013871. Cysteine_rich_secretory.
IPR003582. ShKT_dom.
[Graphical view]
PANTHERiPTHR10334. PTHR10334. 1 hit.
PfamiPF00188. CAP. 1 hit.
PF08562. Crisp. 1 hit.
[Graphical view]
PRINTSiPR00837. V5TPXLIKE.
SMARTiSM00198. SCP. 1 hit.
[Graphical view]
SUPFAMiSSF55797. SSF55797. 1 hit.
PROSITEiPS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
PS51670. SHKT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of the cDNA for androgen-dependent sperm-coating glycoproteins secreted by the rat epididymis."
    Brooks D.E., Means A.R., Wright E.J., Singh S.P., Tiver K.K.
    Eur. J. Biochem. 161:13-18(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Epididymis.
  2. "Molecular cloning of complementary deoxyribonucleic acid for an androgen-regulated epididymal protein: sequence homology with metalloproteins."
    Charest N.J., Joseph D.R., Wilson E.M., French F.S.
    Mol. Endocrinol. 2:999-1004(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCRIS1_RAT
AccessioniPrimary (citable) accession number: P12020
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 11, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.