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Protein

cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase A

Gene

pdsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphodiesterase which displays a preference for cAMP over cGMP. Involved in the degradation of extracellular cAMP. Maintains the responsiveness of cells to the chemoattractant cAMP during the aggregation phase of development.1 Publication

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Enzyme regulationi

Inhibited by dithiotreitol (DTT).1 Publication

Kineticsi

cAMP/cGMP selectivity of 3.
  1. KM=0.8 µM for cAMP1 Publication
  2. KM=1.8 µM for cGMP1 Publication
  1. Vmax=700 pmol/min/mg enzyme with cAMP as substrate1 Publication
  2. Vmax=490 pmol/min/mg enzyme with cGMP as substrate1 Publication

GO - Molecular functioni

  • 3',5'-cyclic-AMP phosphodiesterase activity Source: dictyBase
  • 3',5'-cyclic-GMP phosphodiesterase activity Source: dictyBase
  • cAMP binding Source: dictyBase
  • cGMP binding Source: UniProtKB-KW

GO - Biological processi

  • aggregation involved in sorocarp development Source: dictyBase
  • cAMP catabolic process Source: dictyBase
  • cGMP catabolic process Source: dictyBase
  • negative regulation of cAMP biosynthetic process Source: GO_Central
  • negative regulation of cAMP-dependent protein kinase activity Source: GO_Central
  • negative regulation of glucose mediated signaling pathway Source: GO_Central
  • regulation of cAMP-mediated signaling Source: GO_Central
  • response to cAMP Source: dictyBase
  • response to catechin Source: dictyBase
  • response to differentiation-inducing factor 1 Source: dictyBase
  • response to dithiothreitol Source: dictyBase
  • slug development involved in sorocarp development Source: dictyBase

Keywordsi

Molecular functionHydrolase
LigandcAMP, cAMP-binding, cGMP, cGMP-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SABIO-RKiP12019

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase A (EC:3.1.4.35, EC:3.1.4.53)
Short name:
PDEase A
Alternative name(s):
3',5'-cyclic-nucleotide phosphodiesterase
Short name:
3':5'-CNP
Phosphodiesterase 1
Short name:
DdPDE1
cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 1
Gene namesi
Name:pdsA
Synonyms:pde1, pdeA
ORF Names:DDB_G0285995
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 4, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0285995 pdsA

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: dictyBase
  • extracellular region Source: dictyBase
  • extracellular space Source: UniProtKB-SubCell
  • plasma membrane Source: dictyBase

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000002335224 – 49Sequence analysisAdd BLAST26
ChainiPRO_000002335350 – 452cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase AAdd BLAST403

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi101N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi277N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP12019

Expressioni

Inductioni

Up-regulated by Pseudomonas aeruginosa, PA14 strain infection but not by Pseudomonas aeruginosa, PA01 strain.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0219974

Structurei

3D structure databases

ProteinModelPortaliP12019
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi28 – 35Poly-Asp8

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5212 LUCA
InParanoidiP12019
KOiK01120
OMAiCHDNLIS
PhylomeDBiP12019

Family and domain databases

CDDicd07735 class_II_PDE_MBL-fold, 1 hit
Gene3Di3.60.15.10, 1 hit
InterProiView protein in InterPro
IPR024225 cAMP-PdiesteraseII_CS
IPR000396 Pdiesterase2
IPR036866 RibonucZ/Hydroxyglut_hydro
PANTHERiPTHR28283 PTHR28283, 1 hit
PfamiView protein in Pfam
PF02112 PDEase_II, 1 hit
PIRSFiPIRSF000962 Cyc_nuc_PDEase, 1 hit
PRINTSiPR00388 PDIESTERASE2
SUPFAMiSSF56281 SSF56281, 2 hits
PROSITEiView protein in PROSITE
PS00607 PDEASE_II, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNKKLISL LLLIFIILNI VNSHQQEDCD DDDEDIGISA ERSERRSVKN
60 70 80 90 100
SNDGSNFYNL NDYYTPENWN YYSGSFATKD CRDASYITIP LGTTGGLDEG
110 120 130 140 150
NLSSFLLTKK GSNLFIALDA GTVWQGVRRL TTFKYFNTLF NITYPSWAVL
160 170 180 190 200
PEQRTSWFLK NHVMSYFIGH SHLDHVGGLI LVSPEDYLAK NWIDVQPPIN
210 220 230 240 250
NGIMGLIRKL GFKPTDFTSS SILQKKTIMG LPSTINSIST NLFNNQVWPN
260 270 280 290 300
LPSFGRYQYF SLASGIEYPF TELVPYNATT MSLVANEFPF SVKVKPFELC
310 320 330 340 350
HDNLISTSFL FTDSISGEQI AFFSDTGVPS SVACDWEGKI YAVWKQIKID
360 370 380 390 400
KLKAIYIETS FPNNTPDSAM FGHLRPRDVM KLMDQLLVQS IQTSPPMTNL
410 420 430 440 450
KHVKLIIEHI KPQVAEDPNG WTTQRVIYQQ LKEANNNGVR IIIPNQGDPI

CI
Length:452
Mass (Da):51,093
Last modified:February 1, 1996 - v2
Checksum:iA8F3C190D4603BD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68 – 69NW → LT in AAA33238 (PubMed:3020155).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02628 mRNA Translation: AAA68447.1
M23449 Genomic DNA Translation: AAA63168.1
AAFI02000083 Genomic DNA Translation: EAL64439.1
M15738 mRNA Translation: AAA33238.1
PIRiA32573 A25346
RefSeqiXP_637948.1, XM_632856.1

Genome annotation databases

EnsemblProtistsiEAL64439; EAL64439; DDB_G0285995
GeneIDi8625393
KEGGiddi:DDB_G0285995

Similar proteinsi

Entry informationi

Entry nameiPDE1_DICDI
AccessioniPrimary (citable) accession number: P12019
Secondary accession number(s): Q54ME9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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