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P12019 (PDE1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase A

Short name=PDEase A
EC=3.1.4.35
EC=3.1.4.53
Alternative name(s):
3',5'-cyclic-nucleotide phosphodiesterase
Short name=3':5'-CNP
Phosphodiesterase 1
Short name=DdPDE1
cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 1
Gene names
Name:pdsA
Synonyms:pde1, pdeA
ORF Names:DDB_G0285995
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphodiesterase which displays a preference for cAMP over cGMP. Involved in the degradation of extracellular cAMP. Maintains the responsiveness of cells to the chemoattractant cAMP during the aggregation phase of development. Ref.5

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Enzyme regulation

Inhibited by dithiotreitol (DTT). Ref.6

Subcellular location

Secretedextracellular space. Cell surface Ref.6.

Induction

Up-regulated by Pseudomonas aeruginosa, PA14 strain infection but not by Pseudomonas aeruginosa, PA01 strain. Ref.6

Sequence similarities

Belongs to the cAMP phosphodiesterase class-II family.

Biophysicochemical properties

Kinetic parameters:

cAMP/cGMP selectivity of 3.

KM=0.8 µM for cAMP Ref.5

KM=1.8 µM for cGMP

Vmax=700 pmol/min/mg enzyme with cAMP as substrate

Vmax=490 pmol/min/mg enzyme with cGMP as substrate

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Propeptide24 – 4926 Potential
PRO_0000023352
Chain50 – 452403cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase A
PRO_0000023353

Regions

Compositional bias28 – 358Poly-Asp

Amino acid modifications

Glycosylation1011N-linked (GlcNAc...) Potential
Glycosylation1411N-linked (GlcNAc...) Potential
Glycosylation2771N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict68 – 692NW → LT in AAA33238. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P12019 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: A8F3C190D4603BD1

FASTA45251,093
        10         20         30         40         50         60 
MALNKKLISL LLLIFIILNI VNSHQQEDCD DDDEDIGISA ERSERRSVKN SNDGSNFYNL 

        70         80         90        100        110        120 
NDYYTPENWN YYSGSFATKD CRDASYITIP LGTTGGLDEG NLSSFLLTKK GSNLFIALDA 

       130        140        150        160        170        180 
GTVWQGVRRL TTFKYFNTLF NITYPSWAVL PEQRTSWFLK NHVMSYFIGH SHLDHVGGLI 

       190        200        210        220        230        240 
LVSPEDYLAK NWIDVQPPIN NGIMGLIRKL GFKPTDFTSS SILQKKTIMG LPSTINSIST 

       250        260        270        280        290        300 
NLFNNQVWPN LPSFGRYQYF SLASGIEYPF TELVPYNATT MSLVANEFPF SVKVKPFELC 

       310        320        330        340        350        360 
HDNLISTSFL FTDSISGEQI AFFSDTGVPS SVACDWEGKI YAVWKQIKID KLKAIYIETS 

       370        380        390        400        410        420 
FPNNTPDSAM FGHLRPRDVM KLMDQLLVQS IQTSPPMTNL KHVKLIIEHI KPQVAEDPNG 

       430        440        450 
WTTQRVIYQQ LKEANNNGVR IIIPNQGDPI CI 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and developmental expression of the cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum."
Lacombe M.-L., Podgorski G.J., Franke J., Kessin R.H.
J. Biol. Chem. 261:16811-16817(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs."
Podgorski G.J., Franke J., Faure M., Kessin R.H.
Mol. Cell. Biol. 9:3938-3950(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"Isolation of a cDNA encoding a portion of the cyclic nucleotide phosphodiesterase of Dictyostelium discoideum."
Podgorski G.J., Franke J., Kessin R.H.
J. Gen. Microbiol. 132:1043-1050(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-69.
[5]"Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium."
Bosgraaf L., Russcher H., Snippe H., Bader S., Wind J., Van Haastert P.J.M.
Mol. Biol. Cell 13:3878-3889(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
[6]"Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP."
Bader S., Kortholt A., Van Haastert P.J.M.
Biochem. J. 402:153-161(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, ENZYME REGULATION.
[7]"Precedence temporal networks to represent temporal relationships in gene expression data."
Sacchi L., Larizza C., Magni P., Bellazzi R.
J. Biomed. Inform. 40:761-774(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[8]"Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains."
Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R., Ivens A., Martinez J.L., Escalante R.
BMC Microbiol. 8:109-109(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02628 mRNA. Translation: AAA68447.1.
M23449 Genomic DNA. Translation: AAA63168.1.
AAFI02000083 Genomic DNA. Translation: EAL64439.1.
M15738 mRNA. Translation: AAA33238.1.
PIRA25346. A32573.
RefSeqXP_637948.1. XM_632856.1.

3D structure databases

ProteinModelPortalP12019.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0219974.

Proteomic databases

PRIDEP12019.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0219974; DDB0219974; DDB_G0285995.
GeneID8625393.
KEGGddi:DDB_G0285995.

Organism-specific databases

dictyBaseDDB_G0285995. pdsA.

Phylogenomic databases

eggNOGCOG5212.
OMATINSIST.
PhylomeDBP12019.

Family and domain databases

Gene3D3.60.15.10. 2 hits.
InterProIPR001279. Beta-lactamas-like.
IPR024225. cAMP-PdiesteraseII_CS.
IPR000396. Pdiesterase2.
[Graphical view]
PfamPF02112. PDEase_II. 1 hit.
[Graphical view]
PIRSFPIRSF000962. Cyc_nuc_PDEase. 1 hit.
PRINTSPR00388. PDIESTERASE2.
SUPFAMSSF56281. SSF56281. 2 hits.
PROSITEPS00607. PDEASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDE1_DICDI
AccessionPrimary (citable) accession number: P12019
Secondary accession number(s): Q54ME9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase