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P12019

- PDE1_DICDI

UniProt

P12019 - PDE1_DICDI

Protein

cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase A

Gene

pdsA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Phosphodiesterase which displays a preference for cAMP over cGMP. Involved in the degradation of extracellular cAMP. Maintains the responsiveness of cells to the chemoattractant cAMP during the aggregation phase of development.1 Publication

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Enzyme regulationi

    Inhibited by dithiotreitol (DTT).1 Publication

    Kineticsi

    cAMP/cGMP selectivity of 3.

    1. KM=0.8 µM for cAMP1 Publication
    2. KM=1.8 µM for cGMP1 Publication

    Vmax=700 pmol/min/mg enzyme with cAMP as substrate1 Publication

    Vmax=490 pmol/min/mg enzyme with cGMP as substrate1 Publication

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: dictyBase
    2. 3',5'-cyclic-GMP phosphodiesterase activity Source: dictyBase
    3. cAMP binding Source: UniProtKB-KW
    4. cGMP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cAMP catabolic process Source: dictyBase
    2. cGMP catabolic process Source: dictyBase
    3. response to catechin Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cAMP-binding, cGMP, cGMP-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase A (EC:3.1.4.35, EC:3.1.4.53)
    Short name:
    PDEase A
    Alternative name(s):
    3',5'-cyclic-nucleotide phosphodiesterase
    Short name:
    3':5'-CNP
    Phosphodiesterase 1
    Short name:
    DdPDE1
    cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 1
    Gene namesi
    Name:pdsA
    Synonyms:pde1, pdeA
    ORF Names:DDB_G0285995
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 4, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0285995. pdsA.

    Subcellular locationi

    Secretedextracellular space 1 Publication. Cell surface 1 Publication

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. extracellular region Source: dictyBase
    3. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Propeptidei24 – 4926Sequence AnalysisPRO_0000023352Add
    BLAST
    Chaini50 – 452403cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase APRO_0000023353Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi101 – 1011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi277 – 2771N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP12019.

    Expressioni

    Inductioni

    Up-regulated by Pseudomonas aeruginosa, PA14 strain infection but not by Pseudomonas aeruginosa, PA01 strain.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0219974.

    Structurei

    3D structure databases

    ProteinModelPortaliP12019.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi28 – 358Poly-Asp

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5212.
    KOiK01120.
    OMAiTINSIST.
    PhylomeDBiP12019.

    Family and domain databases

    Gene3Di3.60.15.10. 2 hits.
    InterProiIPR001279. Beta-lactamas-like.
    IPR024225. cAMP-PdiesteraseII_CS.
    IPR000396. Pdiesterase2.
    [Graphical view]
    PfamiPF02112. PDEase_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000962. Cyc_nuc_PDEase. 1 hit.
    PRINTSiPR00388. PDIESTERASE2.
    SUPFAMiSSF56281. SSF56281. 2 hits.
    PROSITEiPS00607. PDEASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12019-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALNKKLISL LLLIFIILNI VNSHQQEDCD DDDEDIGISA ERSERRSVKN    50
    SNDGSNFYNL NDYYTPENWN YYSGSFATKD CRDASYITIP LGTTGGLDEG 100
    NLSSFLLTKK GSNLFIALDA GTVWQGVRRL TTFKYFNTLF NITYPSWAVL 150
    PEQRTSWFLK NHVMSYFIGH SHLDHVGGLI LVSPEDYLAK NWIDVQPPIN 200
    NGIMGLIRKL GFKPTDFTSS SILQKKTIMG LPSTINSIST NLFNNQVWPN 250
    LPSFGRYQYF SLASGIEYPF TELVPYNATT MSLVANEFPF SVKVKPFELC 300
    HDNLISTSFL FTDSISGEQI AFFSDTGVPS SVACDWEGKI YAVWKQIKID 350
    KLKAIYIETS FPNNTPDSAM FGHLRPRDVM KLMDQLLVQS IQTSPPMTNL 400
    KHVKLIIEHI KPQVAEDPNG WTTQRVIYQQ LKEANNNGVR IIIPNQGDPI 450
    CI 452
    Length:452
    Mass (Da):51,093
    Last modified:February 1, 1996 - v2
    Checksum:iA8F3C190D4603BD1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 692NW → LT in AAA33238. (PubMed:3020155)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02628 mRNA. Translation: AAA68447.1.
    M23449 Genomic DNA. Translation: AAA63168.1.
    AAFI02000083 Genomic DNA. Translation: EAL64439.1.
    M15738 mRNA. Translation: AAA33238.1.
    PIRiA32573. A25346.
    RefSeqiXP_637948.1. XM_632856.1.

    Genome annotation databases

    EnsemblProtistsiDDB0219974; DDB0219974; DDB_G0285995.
    GeneIDi8625393.
    KEGGiddi:DDB_G0285995.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02628 mRNA. Translation: AAA68447.1 .
    M23449 Genomic DNA. Translation: AAA63168.1 .
    AAFI02000083 Genomic DNA. Translation: EAL64439.1 .
    M15738 mRNA. Translation: AAA33238.1 .
    PIRi A32573. A25346.
    RefSeqi XP_637948.1. XM_632856.1.

    3D structure databases

    ProteinModelPortali P12019.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0219974.

    Proteomic databases

    PRIDEi P12019.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0219974 ; DDB0219974 ; DDB_G0285995 .
    GeneIDi 8625393.
    KEGGi ddi:DDB_G0285995.

    Organism-specific databases

    dictyBasei DDB_G0285995. pdsA.

    Phylogenomic databases

    eggNOGi COG5212.
    KOi K01120.
    OMAi TINSIST.
    PhylomeDBi P12019.

    Family and domain databases

    Gene3Di 3.60.15.10. 2 hits.
    InterProi IPR001279. Beta-lactamas-like.
    IPR024225. cAMP-PdiesteraseII_CS.
    IPR000396. Pdiesterase2.
    [Graphical view ]
    Pfami PF02112. PDEase_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000962. Cyc_nuc_PDEase. 1 hit.
    PRINTSi PR00388. PDIESTERASE2.
    SUPFAMi SSF56281. SSF56281. 2 hits.
    PROSITEi PS00607. PDEASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and developmental expression of the cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum."
      Lacombe M.-L., Podgorski G.J., Franke J., Kessin R.H.
      J. Biol. Chem. 261:16811-16817(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The cyclic nucleotide phosphodiesterase gene of Dictyostelium discoideum utilizes alternate promoters and splicing for the synthesis of multiple mRNAs."
      Podgorski G.J., Franke J., Faure M., Kessin R.H.
      Mol. Cell. Biol. 9:3938-3950(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "Isolation of a cDNA encoding a portion of the cyclic nucleotide phosphodiesterase of Dictyostelium discoideum."
      Podgorski G.J., Franke J., Kessin R.H.
      J. Gen. Microbiol. 132:1043-1050(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-69.
    5. "Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium."
      Bosgraaf L., Russcher H., Snippe H., Bader S., Wind J., Van Haastert P.J.M.
      Mol. Biol. Cell 13:3878-3889(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    6. "Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP."
      Bader S., Kortholt A., Van Haastert P.J.M.
      Biochem. J. 402:153-161(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, ENZYME REGULATION.
    7. "Precedence temporal networks to represent temporal relationships in gene expression data."
      Sacchi L., Larizza C., Magni P., Bellazzi R.
      J. Biomed. Inform. 40:761-774(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    8. "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains."
      Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R., Ivens A., Martinez J.L., Escalante R.
      BMC Microbiol. 8:109-109(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPDE1_DICDI
    AccessioniPrimary (citable) accession number: P12019
    Secondary accession number(s): Q54ME9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3